Artificial insemination method for increasing conception rate of cows
A conception rate and cow technology, applied in medical science, veterinary equipment, animal delivery, etc., can solve the problems of narrow application range and low conception rate, and achieve the effect of wide range, large coverage and improved breeding efficiency
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Embodiment 1
[0046] Embodiment 1 provides a kind of artificial insemination method that improves cow conception rate, and this method comprises the steps:
[0047] (1) Prepare materials, reagents, and medicines: artificial insemination equipment and equipment, long arm gloves, disposable syringes, normal saline, cloprostinol sodium for injection (Ningbo Sansheng Pharmaceutical Co., Ltd., 0.2mg / bottle), injection Gonadotropin-releasing hormone (Ningbo Sansheng Pharmaceutical Co., Ltd., 100 μg / bottle) and frozen semen (Simmental cattle, packed in thin tubes) were used.
[0048] (2) Cattle herd selection and health check: 15 Simmental cattle were selected that were healthy, with medium to upper-medium body condition, 60-90 days postpartum, and whose reproductive organs returned to normal.
[0049](3) Exogenous hormone treatment and artificial insemination method: 15 cows were intramuscularly injected with GnRH at 7:00-8:00 am on the 0th day, 100μg / head; Perform rectal examination and perform...
Embodiment 2
[0053] Embodiment 2 provides a kind of artificial insemination method that improves cow conception rate, and this method comprises the steps:
[0054] (1) Prepare materials, reagents and medicines: the same as in Example 1 (sperm Holstein cattle).
[0055] (2) Cattle selection and health check: select 16 Holstein cows that are healthy, with medium to medium body condition, 60-90 days postpartum, and whose reproductive organs have returned to normal.
[0056] (3) Exogenous hormone treatment and artificial insemination method: 7:00-8:00 am on the 0th day, 16 cows were intramuscularly injected with GnRH, 100 μg / head; on the 4th day, 2 cows were in estrus, and rectal examination was performed After that, artificial insemination was performed on the morning of the 5th day; at 7:00-8:00 am on the 7th day, the remaining 14 cows were intramuscularly injected with PGF 2α , 0.4mg / head; on the 8th day, 1 cow was in estrus, and after the rectal examination, artificial insemination was pe...
Embodiment 3
[0060] Embodiment 3 provides a kind of artificial insemination method that improves cow conception rate, and this method comprises the steps:
[0061] (1) Prepare materials, reagents, and medicines: the same as in Example 1.
[0062] (2) Cattle herd selection and health check: 12 healthy, medium-to-medium-upper fat condition, 16-20-month-old, unmated Simmental cows were selected.
[0063] (3) Exogenous hormone treatment and artificial insemination method: 7:00-8:00 am on the 0th day, 12 cows were intramuscularly injected with GnRH, 100 μg / head; on the 3rd day, 1 cow was in estrus, and rectal examination was performed After that, artificial insemination was performed on the morning of the fourth day; at 7:00-8:00 am on the seventh day, the remaining 11 cows were intramuscularly injected with PGF 2α , 0.4mg / head; 7:00-8:00 am on the 9th day, 11 cows were intramuscularly injected with GnRH, 100μg / head; the rectal examination began at 7:00 am on the 10th day, and 7 cows had matur...
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