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Method for cultivating corn-tripsacum monosomic addition line by using corn allopolyploid

A technology of allopolyploidy and rubbing grass, which is applied in the fields of botanical equipment and methods, applications, plant gene improvement, etc., can solve the problems of low success rate of distant hybridization and difficulty in using germplasm of closely related species, and achieve work Small amount, simple method, good stability

Active Publication Date: 2017-06-13
SICHUAN AGRI UNIV
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Problems solved by technology

[0006] Aiming at the problems that the success rate of distant hybridization between corn and its closely related species is low, and the germplasm of its close related species is difficult to use, the purpose of the present invention is to provide corn allopolyploids for cultivating corn-trichograss monomer additional lines the use of

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  • Method for cultivating corn-tripsacum monosomic addition line by using corn allopolyploid
  • Method for cultivating corn-tripsacum monosomic addition line by using corn allopolyploid
  • Method for cultivating corn-tripsacum monosomic addition line by using corn allopolyploid

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Embodiment 1

[0046] Embodiment 1: creation of corn-friction grain monomer addition system

[0047] Proceed as follows:

[0048] (1), MTF-1 (Tripsazea creammaizeT., 2n=76) (see figure 1 ) were planted in flower pots (the size of the flower pots was 40 cm in diameter and 35 cm in height); from the end of March, 10 batches were planted in each batch of corn inbred line Mo17 (Zea mays L., 2n=20) at intervals of 3 days, 20 plants were planted in each batch.

[0049] (2) Bagging the MTF-1 ears before silking, and cutting the MTF-1 filaments to 2-3 cm after the MTF-1 ears were silked, and then pollinating MTF-1 with Mo17 as the male parent , repeat pollination for each ear at least 3 times, mark on the bag after each pollination, harvest the seeds when they are mature, and get F 1 generation.

[0050] (3), April 2013, the F obtained in step (2) 1 The generation seeds are planted in the nutrient bowl of the artificial climate box, and germinated under the condition of 28 ℃ and humidity of 70%...

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Abstract

The invention belongs to the field of distant hybridization breeding methods for plants, and discloses a method for cultivating a corn-tripsacum monosomic addition line by using a corn allopolyploid. The method comprises the following steps: hybridizing the corn allohexaploid (Tripsazea creammaize T.; 2n=76) serving as a nonrecurrent parent with a corn (Zea mays L.) serving as a recurrent parent, then carrying out distinguishing and selection of the number of chromosomes and chromosome compositions on a fructification progeny, and finally obtaining the corn-tripsacum monosomic addition line with total 21 chromosomes including 20 corn chromosomes and 1 tripsacum chromosome. According to the method, an effective way for introducing high-quality germplasm of tripsacum into the corn is supplied; furthermore, the monosomic addition line supplies good materials for researches on a gene function; the monosomic addition line is high in stability and can be stably transmitted to the progeny. The method is simple, short in time and high in efficiency.

Description

technical field [0001] The invention belongs to the field of plant distant hybridization breeding methods, and in particular relates to a method for cultivating maize-trichomonad monosomy addition lines by using maize allopolyploids. Background technique [0002] The wild relatives of crops have many excellent genes and characteristics that can be used by cultivated crops, such as resistance to diseases and insect pests, stress resistance, high quality, etc., but due to reproductive isolation, these excellent genes and characteristics are difficult to be utilized by cultivated crops. In order to introduce these exogenous genes into cultivated crops, breeding a series of addition lines, substitution lines or translocation lines is an important means to enrich crop genetic resources. The allologous addition line refers to the aneuploid material in which the chromosome of the foreign species is added to the original genome of the species. According to the number and type of ad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 唐祈林王红林李晓锋李杨赵艳丽李静孙汝龙谢孟林严旭何如钰马行云吴元奇周树峰
Owner SICHUAN AGRI UNIV
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