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Method for Cultivating Maize Allopolyploid by Utilizing Unreduced Gamete Characteristics of Fried Grass

A technology of allopolyploidy and rubbing grass, which is applied in the field of plant distant hybridization, can solve the problems of difficult traits of close relatives of maize, difficulty in producing breakthrough varieties, and narrow genetic basis

Active Publication Date: 2016-05-25
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the narrow genetic basis in current maize breeding, it is difficult to produce breakthrough varieties, and the reproductive isolation between maize relatives makes it difficult to use the excellent traits of maize relatives in maize breeding. The purpose of the invention is to provide a method for cultivating maize allopolyploid using the unreduced gamete characteristics of Trichograss

Method used

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  • Method for Cultivating Maize Allopolyploid by Utilizing Unreduced Gamete Characteristics of Fried Grass
  • Method for Cultivating Maize Allopolyploid by Utilizing Unreduced Gamete Characteristics of Fried Grass
  • Method for Cultivating Maize Allopolyploid by Utilizing Unreduced Gamete Characteristics of Fried Grass

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] Example 1. Cultivation of maize allogenous heptaploids using MTF-1 and the unreduced gamete characteristics of Trichograss

[0103](1) In Huihe Village Farm, Wenjiang District, Chengdu, Sichuan Province, from the end of March, the corn inbred line Mo17 (Zeamays, 2n=20) was planted in 10 batches with 10 days between each batch and 70 plants in each batch , the spacing between plants and rows is 35cm×60cm; in early April, MTF-1 (TripsazeacreammaizeT. Su Yuegui, Master Thesis of Sichuan Agricultural University, 2009. Publicly available from Sichuan Agricultural University) (see figure 1 ) are planted separately in the field, with a row spacing of 2m×2m, a total of 50 plants;

[0104] (2) At the silking stage, before the MTF-1 ear is silked, put the MTF-1 ear in a kraft paper bag, and after the ear is silked, cut the silk to 1 cm, and use Mo17 as the male parent to MTF- 1 Ear pollination, repeat pollination at least 3 times for each ear, mark on the kraft paper bag after e...

Embodiment 2

[0109] Example 2. Cultivation of maize allo-octoploid using MTF-1 and the unreduced gamete characteristics of Trichograss

[0110] Proceed as follows:

[0111] (1) In Huihe Village Farm, Wenjiang District, Chengdu, Sichuan Province, from late March, tetraploid perennial maize 9475 (Zeaperennis9475, 2n=40) was planted in 7 batches (provided free of charge by the Mexico International Maize and Wheat Improvement Center), The interval between each batch is 7 days, and each batch has 10 plants. The tetraploid perennial maize is planted on a single plant, and the distance between plants and rows is 2m×2m; .Su Yuegui, Master Thesis of Sichuan Agricultural University, 2009. The public can obtain it from Sichuan Agricultural University) and planted separately in the field with a row spacing of 2m×2m, a total of 50 plants;

[0112] (2) At the silking stage, before the MTF-1 ear is silked, put the MTF-1 ear in a kraft paper bag, and cut the silk to 2 cm after the ear is silked, and use ...

Embodiment 3

[0117] Example 3, Comparison Test of Determination of Ear and Tassel Characters of Maize Allo-octoploid MTFF-1 and Maize Allohexaploid MTF-1

[0118] Proceed as follows:

[0119] (1) In April 2012, maize allo-octoploid MTFF-1 (Example 2) and maize allohexaploid MTF-1 (provided by Sichuan Agricultural University) were planted on plastic flowers by means of split propagation Pots (caliber 40cm x height 33cm), each material planted 10 pots.

[0120] (2) Determination period and the measured morphological traits

[0121] After the plants entered the tasseling and silking stage, three pots of maize allo-octoploid MTFF-1 and maize allohexaploid MTF-1 were randomly selected, and the filament length, ear length, and inflorescence were measured with a ruler and an Olympus SZX16 fluorescent stereoscope. Long, main stem long floret anther length, main stem short floret anther length, main stem long floret length, main stem short floret length; count the number of spikes on the main ste...

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Abstract

The invention discloses a method for cultivating a corn allopolyploid by using an unreduced gamete characteristic of tripsacum dactyloides, belonging to the field of corn distance hybridization. The method comprises the steps of: hybridizing an MTF-1 (metal-responsive transcription factor) as a female parent with corn or tetraploid perennation corn as a male parent, and then selecting a filial generation plant which is capable of overwintering, namely a new corn allopolyploid, wherein the tiller number of the filial generation plant is more than 15 and the chromosome number of the filial generation plant is a sum of the chromosome number of a female parent and the chromosome number of the male parent. According to the method provided by the invention, the dysgenesis of corn affinis species is overcome and a good inheritance basis is provided for breeding ground-breaking corn species by using the bred corn allopolyploid as a bridge material transfer character. In addition, the method provided by the invention provides a model to breed allopolyploids of other species. The corn allopolyploid bred by the method provided by the invention is perennial, adopts vegetative propagation, and provides a material to corn allopolyploid origin and evolution research and allopolyploid breeding. The method provided by the invention is simple, is short in time, high in efficiency and small in workload.

Description

technical field [0001] The invention belongs to the field of plant distant hybridization methods, and in particular relates to a method for cultivating maize allopolyploids by utilizing the unreduced gamete characteristics of Trichograss grass. Background technique [0002] Polyploidy refers to organisms with three or more chromosome sets in cells, which can be divided into two categories: autopolyploid and allopolyploid. Polyploidy in plants is very common. Many important cultivated crops such as wheat, cotton, peanut, tobacco, sweet potato, potato, etc., and many fruit trees and vegetables are polyploid. Other crops such as corn (Gaut, B.S., etc., Proceeding of the National Academy of Sciences. 1997.94 (13): 6809-6814), soybean (Shoemaker, R. et al., Genetics, 1996, 144 (1): 329-338) and other ancestors also experienced polyploidization during evolution. In the plant kingdom, compared with diploids, polyploids have the effect of increasing cell volume, often showing chara...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/08C12Q1/68
Inventor 唐祈林荣廷昭程明军苏月贵郑名敏李华雄吴元奇周树峰曹墨菊李志龙蒋伟
Owner SICHUAN AGRI UNIV
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