62 results about "Perennation" patented technology

The invention is applicable to the technical field of biotechnology and genetic engineering, and provides an agrobacterium rhizogenes mediated genetic transformation method for hairy roots of blueberries. According to the method, a blueberry perennial stem segment is used as a receptor material for infection, and rapid genetic transformation of the blueberry stem segment is realized through an agrobacterium tumefaciens-mediated method. The method ingeniously bypasses the lengthy process of differentiating the explants to form somatic embryos, and compared with a traditional leaf disc method genetic transformation system, the method has the advantage that the genetic transformation period is remarkably shortened. And the method can be used for mass infection. Even in a non-sterile environment, the method can realize high-efficiency transformation of the gene, and shows the characteristics of simplicity and high efficiency. Therefore, the invention not only provides a new way for genetic transformation of the blueberries, but also provides valuable reference for genetic transformation research and gene function research of the blueberries.

The invention discloses a gene DobHLH14 for regulating and controlling DoSUS2 expression under drought stress to increase the content of dendrobium officinale polysaccharides and application of the gene DobHLH14, and belongs to the technical field of plant genetic engineering breeding, the nucleotide sequence of the gene DobHLH14 is shown as SEQ ID No: 4, and the coded amino acid sequence is shown as SEQ ID No: 5. The DobHLH14 is applied to the improvement of the polysaccharide content of the plant or the breeding of the drought-tolerant transgenic plant. The homologous and heterologous overexpression results of the dendrobium officinale show that the polysaccharide content and drought resistance of the transgenic plant are obviously improved by regulating the expression of the DoSUS2 gene through the DobHLH14 transcription factor of the dendrobium officinale under drought stress, and the method is an excellent method for breeding perennial herbaceous plants with high polysaccharide and drought resistance.

The invention discloses an acid contaminated soil carbon emission reduction method based on a coupled ecological system, which comprises the following steps: adding rice straw biochar into acid contaminated soil, planting plants which are perennial ryegrass, and after the plants are cultivated to a mature period, strengthening the photosynthesis capability of the plants and the carbon sequestration capability of the soil at the same time, so as to improve the carbon sequestration capability of the acid contaminated soil. The negative increase of the CO2 emission cumulant and the remediation of the acidic polluted soil are realized. According to the method, the emission cumulant of CO2 in the acid polluted soil is greatly reduced, the total absorption amount of CO2 reaches up to 193.41 g / m < 2 >-304.41 g / m < 2 >, and the method has the advantages that raw materials are low in cost and easy to obtain, operation is easy, the operation cost is low, secondary pollution is avoided, and the method is suitable for large-scale use and has wide application prospects in the field of carbon emission reduction of the acid polluted soil (such as heavy metal pollution).

The invention belongs to the technical field of ryegrass plant tillering regulation and control methods, and discloses a ryegrass LpZAT12 gene for regulating and controlling plant tillering and application, and the nucleotide sequence of the LpZAT12 gene is as shown in SEQ ID NO: 1; the amino acid sequence is shown as SEQ ID NO: 2; and the nucleotide sequence of the corresponding promoter is as shown in SEQ ID NO: 3. Transgenic ryegrass obtained through a perennial ryegrass genetic transformation system is subjected to phenotypic analysis, and the result shows that the gene inhibits tillering of perennial ryegrass. The discovery of the LpZAT12 gene provides a new gene resource for cultivating a new perennial ryegrass variety with strong tillering ability. The overexpression of the gene can reduce the management cost of the lawn and prolong the management period; knockout of the gene can promote plant tillering, increase plant biomass and improve industrial benefits, and application of the gene has important production practice significance.

The invention provides a method for planting hemerocallis citrina baroni in a cotton sand land, and belongs to the technical field of hemerocallis citrina baroni planting.The method comprises the steps that S1, the cotton sand land is pretreated, organic fertilizer is spread on the surface of the flat land, primary rotary tillage is conducted, and the cotton sand land is leveled; s2, secondary treatment of the cotton sand land: spreading an inorganic fertilizer on the surface of the flat land, performing secondary rotary tillage, and flattening the cotton sand land; s3, ditching and ridging to form furrows; s4, tillering seedlings, selecting perennial daylily clumpy roots without plant diseases and insect pests, tillering 1 / 3-1 / 4 of the daylily clumpy roots as seedlings, and planting the seedlings into the furrows; s5, increasing water and supplementing fertilizer, introducing water and fertilizer into the furrows, or spreading compound fertilizer at the bottoms of the furrows, and then introducing irrigation water. The furrow planting mode of the daylily has good water-saving and soil moisture preservation effects, the yield per mu is increased by about 100-150 kg, the yield per mu is increased by about 25%-37%, the economic benefit is increased, meanwhile, the survival rate of the daylily can be increased, and root division of the daylily root system in the following years is facilitated.

The invention relates to the technical field of plant grafting, in particular to a method for improving the grafting survival rate of malania oleifera, which comprises the following steps: S1, scion preparation: treating branches of excellent perennial wild malania oleifera plants in the field, conveying the branches to an inoculation field, and disinfecting to obtain disinfected branches; s2, stock preparation; s3, grafting: preparing a scion by using the disinfected branch, treating a stock, tightening the stock and a grafting film for tongue grafting of the scion, and smearing a healing agent on a cut of the cut stock and the upper end of the grafting film; s4, management after grafting. Compared with the prior art, the method has the advantages that superior plant branches and artificially cultivated stocks are subjected to tongue grafting, and compared with bud grafting, the yield time of grafted seedlings is shortened; and then the branch of the malania oleifera superior plant is treated immediately when the scion is collected, so that the problems that the activity of the branch is reduced in the transportation process and the formation of the callus of the stock and scion cambium is slow are solved, the grafting survival rate is increased, and the grafted seedling obtaining time is shortened.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a genetic transformation method of perennial elymus nutans. According to the genetic transformation method of elymus nutans, mature embryos serve as explants for inducing callus formation, the method has the advantages that material obtaining is convenient, operation is simple and easy, the supply problem of explant material obtaining is solved, and meanwhile the physiological state of the mature embryos is consistent, so that the repeatability of experiments is good; in addition, the infection liquid contains the acetosyringone, and the acetosyringone (AS, Acetosyringone) can promote the growth of agrobacterium tumefaciens and enhance the activity of the agrobacterium tumefaciens, so that the conversion efficiency is improved; the browning phenomenon of the calluses is effectively inhibited through sterilization treatment, the normal growth of the calluses can be recovered, the transgenic elymus nutans plants are obtained through the technical scheme, and the research of researchers on the gene functions of the elymus nutans can be greatly promoted.

The invention discloses a method for recovering vegetation of a mildly-degraded Coilin grassland. The method comprises the following steps: S1, investigating plant community characteristics and biomass; s2, determining the quality of the forage grass in the plant community; s3, collecting a soil sample; s4, reseeding: grass seeds are reseeded on the mildly degraded Coilin grassland, the reseeded grass seeds are agropyron perennioides and adsura adsura, and the mass ratio of the agropyron perennioides to the adsura adsura is (6-8): (2.5-3.5); the reseeding amount is 10-20 kg / hm < 2 >; s5, fertilization: applying a fertilizer bag, the fertilizer bag being a nitrogen-phosphorus-potassium mixed fertilizer, the nitrogen-phosphorus-potassium mixed fertilizer comprising a nitrogen fertilizer, a phosphorus fertilizer and a potash fertilizer, according to the plant community conditions of the mildly degraded Coilsein grassland, through reseeding and reasonable fertilization, the monoennial and biennial plants, perennial gramineae, hybrid grass and perennial leguminous plants of the Coilsein grassland can all grow, the yield of the Coilsein grassland is increased, and the yield of the Coilsein grassland is increased. And the pH value of the soil tends to be neutral, so that the mildly degraded Coilin grassland vegetation is recovered.

The new and distinct hardy perennial Heliopsis plant named ‘Rays for Days’ with dense, rounded, extensively-branched habit, strong, tall, upright stems that resist lodging. The foliage is medium green and broadly ovate to narrowly deltoid. Freely-flowering double inflorescences in a capitulum with multiple rows of golden-yellow, overlapping, ray florets beginning in early July and continuing for about 6 weeks. The new plant is useful in the landscape en masse, as an accent, in containers or as cut flowers.

The invention discloses a method for creating offspring of buckwheat interspecific hybridization. According to the invention, the immature embryo can be successfully obtained through artificial hybridization by taking wild buckwheat as a female parent and tartary buckwheat as a male parent; in order to solve the problems that the induction rate of an immature embryo on a callus induction culture medium is low and the differentiation rate is low when the callus is subjected to differentiation culture in a differentiation culture medium, the callus induction culture medium and the differentiation culture medium are optimized and screened; the obtained callus induction culture medium and the obtained differentiation culture medium remarkably improve the callus induction rate and the differentiation rate during differentiation culture, regenerated plants are obtained at high frequency, colchicine doubling is performed on the regenerated plants on the basis, and a doubled interspecific hybridization new germplasm is obtained by combining morphological and cytological identification. The method is applied to breeding of high-yield stress-resistant, high-flavone, perennial and other breakthrough new varieties or serves as a hybrid bridge parent, and the excellent characters of stress resistance, high flavone, perennial and the like of wild buckwheat are introduced into buckwheat cultivation.

The present application relates to a kind of controllable release herbicide composition and its preparation method, the controllable release herbicide composition includes active ingredient 1-60 parts, 10-30 parts of load agent with porous structure, 10-20 parts of water-absorbing expansion material and 40-60 parts of solvent;Wherein, at least part of the active ingredient and at least part of the water-absorbing expansion material are located inside and surface of the load agent.The herbicide composition of the present application can achieve the effect of controllable slow release, improve the storage effect of herbicide composition under dry and wet conditions, improve the use efficiency of herbicide composition, improve the foliage concentration of vegetation, reduce the invalid degradation of active ingredient, improve the killing effect of herbicide composition on tuberous plants, perennial herbaceous plants and other various plants.

The invention provides a grass checkered sand barrier with a seed bank and a planting method and application of the grass checkered sand barrier, and belongs to the technical field of environmental governance. The planting method of the grass checkered sand-protecting barrier comprises the following steps: mixing and weaving the overground part of a drought-resistant and barren-resistant grass shrub perennial plant, a xerophyte cuttage plant branch and rice straw straws into a grass-protecting barrier, and planting the grass checkered sand-protecting barrier; the drought-tolerant and barren-tolerant grass shrub perennial plants are selected from at least two of ladywood, lespedeza floriflora, lespedeza tenuifolia and leymus chinensis at the upper part of a mowing field in a fruit maturation period; the xerophyte cutting plant is selected from one or more of yellow willow, salix psammophila and tamarix chinensis. In the grass checkered sand barrier, the rice straw plays a role in protection, the cutting branches have a cutting survival function, seeds carried by perennial plants can germinate and grow plants in good time under the shielding of the grass checkered sand barrier, vegetation in sand or desert areas can be recovered, the vegetation coverage and density are improved, and the survival rate of the perennial plants is increased. The vegetation coverage can be improved by more than 30% compared with that of an exposed plot after planting for 2 years.

The invention discloses a method for preventing and treating an invasive plant phytolacca oryzae by using diversity of local plants, and belongs to the technical field of comprehensive treatment of invasive plants. The method for preventing and treating the invasive plant pokeberry root by using the diversity of the local plants comprises the following steps: S1, cutting the overground part of the pokeberry root, and cleaning the underground perennial root system of the pokeberry root; s2, covering the soil surface of the invasion area with a new soil layer which is not invaded by phytolacca petiolata; s3, selecting various common local plant seeds, and sowing the seeds in the area treated in the step S2. The method provided by the invention is simple and clear, and comprises four steps of cutting, crushing, covering and planting, so that the aim of blocking the invasive plant phytolacca pendula by the biological diversity is fulfilled.

The invention belongs to the technical field of gene engineering, and discloses application of a dehydrin gene in plant drought resistance. The dehydrin gene is separated and extracted from perennial ryegrass and is specifically an LpDHN1 gene, and the nucleotide sequence of the dehydrin gene is shown as SEQ ID NO: 1. The dehydrin gene positively regulates the drought resistance of the plant, and the drought resistance of the plant can be improved by overexpressing the dehydrin gene. After the LpDHN1 gene is heterologously expressed in arabidopsis thaliana, the survival rate of a transgenic plant under drought stress is remarkably improved, and the ion permeability and malondialdehyde content of leaf cells of the transgenic plant under the drought stress condition are remarkably reduced. The invention provides a novel germplasm material for cultivation of drought-resistant plant varieties and improvement of drought-resistant characters of plants.

Provided are a device for processing tubers or snails and a method for processing tubers or snails. The device for processing tubers or snails according to the present invention processes a tuber A or a snail associated with a perennial weed buried in the soil of a paddy field, and has: a digging part 10 for scooping up the tuber A or the snail together with the soil; a rotary member 13 having claw parts that rotate above the digging part 10; a conveyor part 20 for roughly separating, from the soil, the tuber A or the snail scooped up by the digging part 10; and a roller part 30 for crushing the tuber A or the snail conveyed from the conveyor part 20. By forming the digging part 10 into a curved surface shape along a rotation track of the rotary 13, the amount of mud conveyed to the conveyor part 20 can be controlled to facilitate separation of the tuber A or the snail from the mud, thereby allowing rapid and efficient processing of the tuber or the snail.

The invention discloses a method for ecologically restoring moderately degenerated Coilsein grassland. The method comprises the following steps: S1, investigating the degeneration condition of the Coilsein grassland; s2, investigating plant community characteristics and biomass; s3, determining the quality of the forage grass in the plant community; s4, collecting a soil sample; s5, reseeding, wherein the ratio of the agropyron crocea to the astragalus adsurgens is (2.5-3.5): 1; according to the method, appropriate grass seeds and fertilizer bags are screened out according to the plant community conditions of the moderately-degenerated Coerquin grassland, reseeding and fertilization are carried out, and finally the moderately-degenerated Coerquin grassland monoennial plants, biennial plants, perennial gramineae, hybrid grass and perennial leguminous plants all grow, so that the yield of the Coerquin grassland is increased, the yield of the Coerquin grassland is increased, and the yield of the Coerquin grassland is increased. The pH value of the soil tends to be neutral, the total nitrogen content of the soil is increased through fertilization, and moderately degenerated Coilin grassland vegetation is recovered.

The present invention discloses a switchgrass transcription factor gene PvC3H12 for improving plant cold tolerance and its application, belonging to the technical field of genetic engineering. The CDS sequence of the switchgrass transcription factor gene PvC3H12 is as shown in SEQ ID NO.7. This gene is localized in the nucleus and exhibits obvious transcriptional activation activity in yeast, indicating that PvC3H12 is a transcriptional activator. Further research found that overexpression of PvC3H12 significantly improved the cold tolerance of switchgrass. The acquisition of the switchgrass transcription factor gene PvC3H12 of the present invention is expected to improve the cold tolerance of multiple perennial warm-season plants and provide a theoretical basis for cultivating plants with high cold tolerance.

The new and distinct hardy perennial Nepeta plant named ‘Lemon Purrfection’ has a dense, compressed mound, with compact habit and arching to horizontal stems. The new plant begins flowers in late spring and continues and repeats until autumn frost without deadheading. The flowers are bluish-lavender with purple spots and persistent mauve calyces just above the complementing bright chartreuse-yellow, cordate, fragrant foliage. The new plant is useful in the landscape en masse, as an accent, or in containers to bring color and fragrance to a garden and attract butterflies and hummingbirds.

‘NARUO1920’ is a new plant of perennial Zoysia grass plant which is distinguishable from other Zoysia plants know to the Inventor by having a very short leaf length, fine leaves, and high creeping ability.

The invention relates to the field of agricultural pesticides, in particular to a herbicide composition and application thereof, and the herbicide composition comprises the following components in percentage by mass: 5-70% of a weeding drug, 1-27% of a dispersing agent, 0.5-50% of modified sodium bentonite and 5-28% of a wetting agent. The herbicide composition prepared by the invention is relatively good in intersolubility, the components stably coexist, the weeding spectrum is expanded, and the herbicide composition has a good synergistic effect on preventing and treating annual and perennial gramineae and broadleaf weeds.

This invention belongs to the field of microbial technology and discloses a synthetic microbial community for enhancing biological nitrogen fixation in karst grasslands, its construction method, and its application. The synthetic microbial community includes... Sinorhizobium americanum , Sionrhizobium sp. as well as Bacillus cereus Wherein: the Sinorhizobium americanum The accession number is GDMCC NO: 67796; Sionrhizobium sp. The accession number is GDMCC NO: 67797; Bacillus cereus The accession number is GDMCC NO: 67798. For leguminous forage grasses that require repeated sowing, the seeds are treated with a synthetic microbial community before being sown in the field; for perennial leguminous forage grasses that have already been planted, the synthetic microbial community is diluted with water and sprayed onto the surface of adjacent leguminous forage grasses. This invention screens out suitable and efficient root nodule endophytic bacteria in karst areas and constructs a synthetic microbial community to enhance the biological nitrogen fixation of leguminous forage grasses. It clarifies the technical details of the practical application of the synthetic microbial community in the field. The application of this technology can significantly improve the biological nitrogen fixation rate of leguminous forage grasses, significantly reduce the amount of nitrogen fertilizer used in forage grasses, and significantly increase forage grass yield.

The application provides a method for ecological restoration of a degenerated alpine meadow of Stellera chamaejasme, and the method is as follows: arranging a sunshade net to inhibit photosynthesis of Stellera chamaejasme, improve photosynthesis of constructive species with low light saturation point, and reduce water evaporation on the ground, so as to create favorable competition conditions for shallow-rooted constructive species; removing seeds before fruit drop through fruiting cutting to prevent sexual reproduction and spread of Stellera chamaejasme; mixing annual and perennial plants to form a competitive relationship with Stellera chamaejasme for light and water resources, and to attack Stellera chamaejasme from top and bottom; and appropriately rotating grazing to moderately interfere with the ecological system and maintain stability of the ecological system, so as to achieve the purpose of restoration of the alpine meadow. The method is based on the botanical characteristics of Stellera chamaejasme, does not introduce exogenous chemical toxic substances in the implementation process, and has low implementation cost, so it is hopeful to be applied on a large scale in restoration of the degenerated alpine meadow of Stellera chamaejasme.

The invention provides a method for constructing a perennial plant and application of the perennial plant. The invention discloses a new function of a gene, the gene is an FLC-like MADS-box gene and participates in regulation and control of perennial development of plants, and an annual plant is changed into a perennial plant. According to the method, the perennial plants can be obtained within a short time, and compared with a traditional mutagenesis method, the workload is small, the operability is good, and the method can be directionally completed through a single gene or multiple genes. Compared with a hybridization method, the method disclosed by the invention is small in workload and is not influenced by hybridization incompatibility among species. And perennial plants can be obtained in a short time.

The application discloses a kind of perennial wild peanuts as grass fire dragon fruit cultivation method, S1.selects ground, ground and ridge;S2.punching post, planting pitaya seedling;S3.transplanting wild peanuts: wild peanuts branch according to travel distance 20~25cm, plant distance 15~20cm in the strip ditch of pitaya garden;S4.water and fertilizer management;S5.mowing: when wild peanuts flower basically withers, leaf color presents old green as a whole, that is, the aboveground part of wild peanuts is mowed with mower and directly returned to field, underground part rhizome is retained and respectively in March, June, September and November every year, a total of 4 times mowing.The application adopts perennial rhizome area group wild peanuts A.glabrata (PI262801) intercropping cultivation pitaya, and the pitaya garden benefits for many years after grafting planting once, can inhibit weed growth, compared with other green manure, wild peanuts have good effect on deep soil improvement, and the effect is remarkable.

The invention provides a wild flower combination for resisting alien plant invasion and application thereof, and belongs to the professional field of landscape gardens and ecology. The wild flower combination comprises the following flower species: laces, common spilanthes, lythrum salicaria, pennisetum alopecuroides, perennial salvia, rhododendron simsii, sanguisorba officinalis, polypogon fugax, salvia plebeian, roegneria erecta, linaria lingua, salvia palustris, echinacea purpurea, sessileflower desmodium, corn poppy, cirsium setosum, hispid arthraxon, duchesnea indica, potentilla serrata, selfheal, dandelion and herba violae. By considering the reasonable configuration of plants with different heights, the phenological difference of perennial root plants and annual and biennial plants and the competitive relationship between the planted plants and weeds, a high-density vegetation area is created, ecological design is performed from three dimensions of space, time and functions, the alien plant invasion resistance of the wild flower combination community is remarkably enhanced, and the survival rate of the wild flower combination community is increased. Meanwhile, the biodiversity protection and the landscape effect improvement are both considered.

The invention discloses a vegetation reconstruction structure and method for a damaged side slope in a high-cold and high-altitude area. The method comprises the following steps: S1, trimming the surface of the side slope; s2, a first hanging net is laid on the trimmed slope surface; s3, a greening base material mixture is sprayed and sown on the first hanging net; s4, laying a second hanging net on the greening base material mixture; s5, a greening base material mixture containing grass seeds is sprayed and seeded on the second hanging net; s6, covering non-woven fabric after spray seeding. According to the method, vegetation reconstruction and restoration of the damaged slope in the high-cold and high-altitude area can be achieved, the restoration effect is natural, and the method can adapt to the special geographical environment of the high-cold and high-altitude area and coordinate with regional geodetic landscapes; the spray-seeding plants adopt elymus nutans, siberian wildrye and annual ryegrass, are creative combination test results of grass seeds, can well adapt to the local environment, are good in germination and growth performance, enhance the stability and anti-jamming capability of an ecological system, adopt the combination of annual and perennial grass seeds, give consideration to both the short-term effect and the long-term effect, and are suitable for large-scale popularization and application. And forward succession of plant communities is facilitated.

The ecological pile device with the animal and plant habitat building function comprises ecological pile units, a conical head is integrally formed at the bottom of a pile body, a pile tip is arranged at the bottom end of the conical head, a limiting ridge is integrally formed at the top of the pile head, an ecological planting groove is formed in the middle of the top of the limiting ridge, and the ecological planting groove is formed in the middle of the top of the limiting ridge. Herbaceous plants are planted in the ecological planting grooves, every two adjacent sets of ecological pile units are connected and assembled through stabilizing pieces, pile bodies are subjected to thread treatment, the insertion convenience of the pile bodies is improved, meanwhile, a soil body and the ecological pile units which are used in practice are firmer, the planting grooves are formed in the tops of the ecological pile units, and the ecological pile units are more convenient to use. Perennial herbaceous plants can be planted, the two adjacent ecological pile units are fixed through the stabilizing pieces, the mounting stability of the ecological pile units is improved, meanwhile, the uniformity of mounting of the multiple ecological pile units can be achieved, and the adjacent ecological pile units have the supporting effect.

A new and distinct variety of peony plant with light pink flowers with a creamy yellow shade; a medium-sized perennial herbaceous shrub with vigorous yet compact growth that is floriferous due to numerous secondary buds and that propagates very easily by root clump divisions, is disclosed.

A central connecting element with an internal thread for fixing a clip-on, laterally equipped plant support bracket by means of an Allen screw, which is axially slidable on a vertical rod and can be locked in place. (See drawing 1)

To provide a new technology that enables reduction of the time required for perennial plants to reach flowering.SOLUTION: The method for promoting flowering comprises a step of grafting in which a portion including a growth point of a juvenile plant body of a perennial plant that has not formed a flower bud is used as a scion 1, and a plant that has formed a flower bud is used as a rootstock 2, wherein the juvenile plant body used as the scion 1 and the plant used as the rootstock 2 are plants belonging to the same family.SELECTED DRAWING: Figure 2