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Tissue culture and rapid propagation method for epimedium wushanense

A technology of Epimedium and Huoshan group, which is applied in the field of tissue culture and rapid propagation of Epimedium wushanense, can solve the problems of low natural reproduction rate, low germination rate of reproduction and reduction of wild resources in wild Epimedium wushanense, and achieve sustainable utilization , low reproductive germination rate, and the effect of protecting wild resources

Inactive Publication Date: 2015-06-03
黎有辉
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the low natural reproduction rate of wild Epimedium wushanense, slow growth and people's predatory digging, the wild resources are decreasing day by day, which is far from meeting the needs of economic development and clinical medicine in domestic and foreign markets. Although artificial cultivation has been carried out and established Standardized planting bases, but the cultivation of seedlings in production is mainly through traditional methods such as division propagation and rhizome propagation. Due to the tradition of viruses from generation to generation, the quality declines and the yield is low.
In addition, the seeds of Epimedium are poorly developed, have the characteristics of deep dormancy, low reproductive germination rate, and slow growth, which seriously restricts the reproductive coefficient and breeding cycle of its sexual reproduction.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Disinfection of explants: Take healthy Epimedium wushanense buds as explants, scrape off the bud marks, add detergent and bleach for 30 minutes, then rinse under tap water for 30 minutes, then move to the ultra-clean workbench, use Soak in 75% alcohol for 30s, then use 0.1% HgCl 2 Disinfect for 4 minutes, peel off the outer layer of scales after washing with sterile water, and then use 0.1% HgCl 2 Soak for 2 minutes, rinse the explants with sterile water until there is no foam, place them on sterile filter paper to blot the surface moisture, and peel off the bud tip tissue for later use.

[0018] (2) Adventitious bud induction: peel off the outer protective layer of the sterilized buds, and inoculate the exposed green bud tips into the induction medium for adventitious bud induction culture. After inoculation, it is placed in the light for 13 hours a day, the light intensity is 1000lx, the culture temperature is 25°C, and the relative air humidity is 75%. After 15...

Embodiment 2

[0023] (1) Disinfection of explants: Take healthy Wushan Epimedium buds as explants, scrape off the bud marks, add detergent and bleach for 45 minutes, then rinse under tap water for 35 minutes, then move to the ultra-clean workbench. Soak in 75% alcohol for 50s, then use 0.1% HgCl 2 Disinfect for 6 minutes, peel off the outer scales after washing with sterile water, and then use 0.1% HgCl 2 Soak for 4 minutes, rinse the explants with sterile water until there is no foam, place them on sterile filter paper to blot the surface moisture, and peel off the bud tip tissue for later use.

[0024] (2) Adventitious bud induction: peel off the outer protective layer of the sterilized buds, and inoculate the exposed green bud tips into the induction medium for adventitious bud induction culture. After inoculation, it is placed in the light for 14 hours a day, the light intensity is 1500lx, the culture temperature is 27°C, and the relative air humidity is 75%. After 15 days of cultivat...

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Abstract

The invention discloses a tissue culture and rapid propagation method for epimedium wushanense. The epimedium wushanense belongs to an epimedium brevicomium herbaceous perennial herb plant, and is a conventional tonifying traditional Chinese medicine of China. Artificial cultivation is developed and a standardized planting base is built, but cultivated seedlings on production are mainly propagated through conventional modes of division propagation, rhizome propagation and the like, and the quality is reduced due to successive-generation transmission of viruses. An epimedium wushanense tender shoot is taken as an explant, an epimedium wushanense in-vitro replanting plant is successfully obtained by adventive bud induction culture, multiplication culture, rooting culture, acclimatization and transplantation, and the like, an epimedium wushanense tissue culture and rapid propagation system is established, and a large number of seedlings can be obtained within a short time. The tissue culture and rapid propagation method is of great significance in protecting epimedium wushanense wild resources, guaranteeing a medicine source and realizing sustainable utilization of epimedium wushanense.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of Epimedium wushanense. Background technique [0002] Wushan Epimedium ( Epimedium wushanense ) is a perennial herbaceous medicinal plant of Epimedium genus Berberidaceae. It is a traditional tonic Chinese medicine in my country and one of the most widely used and oldest Chinese medicines in China. Epimedium Wushan is rich in isopentenyl-substituted flavonoids, which have the functions of nourishing kidney yang, strengthening muscles and bones, and dispelling rheumatism. It is used for the treatment of diseases such as kidney yang deficiency, impotence and nocturnal emission, flaccidity of muscles and bones, rheumatic arthralgia, numbness and spasm, menopausal dizziness and other diseases. In recent years, studies have found that Epimedium also has the effects of anti-aging, improving immune fu...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 黎有辉
Owner 黎有辉
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