331 results about "Epimedium elatum" patented technology

The invention relates to the technical field of disease monitoring, in particular to a disease monitoring method and system in the epimedium growth process, and the method comprises the steps: deploying a multi-source collection device in an epimedium planting area, and obtaining plant images in real time according to a preset time interval, the plant images including RGB images and multispectral images; performing image preprocessing on the acquired RGB image to obtain a standard plant image; acquiring plant growth time, performing morphological analysis on the standard plant image to extract growth characteristic parameters, and inputting the growth characteristic parameters and the growth time into a pre-constructed plant growth stage prediction model to acquire the epimedium growth stage. According to the disease monitoring method for the epimedium growth process provided by the invention, by fusing RGB and multispectral images, accurately extracting key features such as diseased leaves and areas, colors and shapes of the diseased leaves, and inputting a disease prediction model in a growth stage, intelligent discrimination of epimedium disease types and severity levels can be realized.

The invention discloses an anti-aging herba epimedii extract. The anti-aging herba epimedii extract is prepared by performing microbial fermentation on herba epimedii powder. The invention further discloses a preparation method of the anti-aging herba epimedii extract and application of the anti-aging herba epimedii extract in preparation of anti-aging drugs, food and health care products. According to the invention, through special plant lactobacillus and a fermentation process, efficient conversion of main glycoside components in herba epimedii is realized, the contents of icariin and epimedin C are reduced, the contents of secondary glycoside I and secondary glycoside II are remarkably increased, and new icaritin is generated. The herba epimedii extract disclosed by the invention has the effects of resisting aging and prolonging the service life.

The invention relates to the technical field of biology, in particular to an SSR molecular marker primer group for identifying the variety of herba epimedii, a kit and application. The core primers of the SSR molecular marker disclosed by the invention comprise 823, 840, 841, 842, 854 and 866, and the primers can be used for identifying the herba epimedii arrow leaf No.1, the herba epimedii arrow leaf No.2 and the herba epimedii soft hair No.1. The SSR molecule can be used for researching the genetic diversity, genetic relationship and genetic distance of epimedium, and respectively establishing molecular identity cards of three new epimedium varieties, namely epimedium arrow leaf No.1, epimedium arrow leaf No.2 and epimedium soft hair No.1, so that a foundation is laid for genetic map construction, excellent character marking and the like; meanwhile, a scientific basis is provided for constructing a molecular breeding system of a new variety (line) of herba epimedii in the later period.

The invention relates to the technical field of traditional Chinese medicine effective component extraction, and provides an efficient extraction and purification process and application of icariin in plateau herba epimedii, the efficient extraction and purification process of icariin in plateau herba epimedii comprises the following steps: S1, epimedium leaves are crushed and sieved to obtain epimedium powder; s2, adding an ethanol solution into the herba epimedii powder, then adding a compound enzyme, performing ultrasonic treatment, inactivating the enzyme, performing suction filtration, and collecting filtrate to obtain an icariin crude extract; s3, diluting the crude icariin extract, adjusting the pH value to 5-6, carrying out chromatographic purification and elution, and collecting the eluate; s4, performing vacuum concentration on the eluent, and performing spray drying to obtain a pure icariin product; the compound enzyme comprises cellulase, pectinase and alpha-mannosidase in a mass ratio of 3: 1: (0.2-1.0). By means of the technical scheme, the problem that in the prior art, the extraction rate of icariin in plateau herba epimedii is low is solved.

The invention discloses a method for improving the quality of epimedium seeds and application thereof, and the method comprises the following steps: S1, carrying out fixed-plant and fixed-point observation in a homogeneous garden, recording and observing the flowering habit and synchronism of epimedium delavayi, including the flowering period time and pollination insect species, and observing the flower characteristics; stigma pollination is determined through pollen activity detection, and a basis is provided for subsequent artificial isolated pollination; s2, performing artificial isolation pollination in the flowering phase of the epimedium villosum plants, and using an isolation closing device to prevent cross-pollination. According to the method for improving the quality of the herba epimedii seeds and the application of the method, the flowering rule of the herba epimedii is determined through homogeneous garden transplanting experiments, the pollen viability is dynamically monitored in combination with a TTC staining method, an artificial isolation pollination device and an operation process are designed, and the quality of the herba epimedii seeds is improved. The method has the following technical effects that the seed purity is improved, wherein the seed purity after artificial isolation pollination reaches 90% or above, and the genetic background consistency is remarkably improved.

The invention discloses a preparation method of granules for treating breast and thyroid nodules and canceration, which comprises the following steps: S1, raw material preparation: preparing 199g of codonopsis pilosula, 332g of astragalus membranaceus, 149g of glossy privet fruit, 100g of herba epimedii, 166g of selfheal, 100g of uniflower swisscentaury root, 100g of rhizoma pinellinae praeparata, 100g of curcuma zedoary, 199g of salvia chinensis, 332g of selaginella doederleinii hieron, 100g of curcuma aromatica and 149g of cortex albiziae according to a mass ratio; s2, adding water to decoct the twelve medicines twice, adding 8 times by mass of water for the first time, extracting for 2.0 hours, and filtering; s3, adding 6 times by mass of water for the second time, extracting for 1.5 hours, filtering, and combining filtrates; s4, concentrating under reduced pressure to obtain clear paste with the relative density of about 1.05-1.10 (60 DEG C); and S5, performing vacuum drying, crushing into fine powder, adding dextrin and 10g of stevioside, uniformly mixing, granulating with 75% by volume of ethanol, drying, preparing into 1000g, and packaging, thereby obtaining the product.

The invention discloses a radix astragali, rhizoma polygonati and ligustrum lucidum ait traditional Chinese medicine preparation for treating myelosuppression as well as a preparation method and a detection method thereof. The preparation is prepared from 50-150 parts of radix astragali, 50-150 parts of wine-processed rhizoma polygonati, 25-75 parts of wine-processed ligustrum lucidum ait, 50-150 parts of blanched rhizoma drynariae, 50-150 parts of radix trichosanthis and 25-75 parts of herba epimedii. The optimal extraction process and refining process are screened out based on effective components and pharmacological activity through a large number of tests, and the optimal preparation process of the Shuanghuangliangwhitening oral liquid and other traditional Chinese medicine preparations is obtained through stability investigation and verification. According to the invention, a thin layer chromatography is adopted, the four medicinal materials of astragalus membranaceus, glossy privet fruit, rhizoma drynariae and herba epimedii are respectively subjected to thin layer chromatography research, and methodology research shows that the method is stable and feasible. The invention establishes a content determination method for astragaloside, calycosin-7-glucoside, specnuezhenide and icariin, establishes a quality standard, and has important significance for ensuring the clinical curative effect and safety of astragaloside, calycosin-7-glucoside, specnuezhenide and icariin.

The invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to a kidney-tonifying and liver-nourishing compound preparation and a preparation method thereof. The compound preparation comprises 5-15 parts of roasted herba epimedii, 5-15 parts of fructus cnidii, 50-70 parts of roasted radix astragali and 10-30 parts of pulp of dogwood fruit. The traditional Chinese medicine composition has remarkable curative effects on various diseases and chronic strain caused by deficiency of liver and kidney and deficiency of blood and essence, such as dizziness, blurred vision, fatigue, swelling and pain of waist, back and limbs, dry mouth and eyes, limb ache and weakness, irregular menstruation, incapability of holding weight or hemiplegia and the like, and has a very good application prospect.

The invention belongs to the technical field of quality control of traditional Chinese medicinal materials, and particularly relates to a quality control method of a herba epimedii medicinal material. The HPLC characteristic chromatogram containing nine characteristic peaks is established, the ratio of the peak area of the characteristic peaks to the total area is more than 70%, the characteristic chromatogram of the herba epimedii medicinal material is constructed, and true and false identification is carried out from the perspective of multiple indexes. During quality evaluation, the peak areas of the nine characteristic peaks are used as input information, the similarity between the sample and the high-quality sample is evaluated from the perspective of multi-dimensional regression analysis, the internal quality of the herba epimedii medicinal material is reflected more scientifically and comprehensively, and the method has better comprehensiveness and integrity compared with traditional indexes.

The invention belongs to the field of pharmaceutical composition preparation, and particularly relates to a traditional Chinese medicine composition for invigorating kidney and strengthening yang and a preparation method thereof, the traditional Chinese medicine composition is composed of the following raw materials by weight: 50 g of epimedium, 50 g of cistanche, 50 g of cordyceps sinensis, 50 g of cynomorium songaricum, and 50 g of eucommia ulmoides. The weight ratio of the herba epimedii to the cistanche to the cordyceps sinensis to the cynomorium songaricum to the eucommia ulmoides is the same and is 1: 1: 1: 1: 1. Therefore, through the raw material combination design that the specific medicinal parts of the raw materials are defined and the weight ratio of the herba epimedii to the herba cistanche to the cordyceps sinensis to the cynomorium songaricum to the eucommia ulmoides is 1: 1, the core effective components of the raw materials are distributed in a balanced proportion, and the effect of synergistically constructing a kidney-tonifying essence-strengthening body-strengthening stable effect system is achieved; therefore, the problems that in the background technology, an existing kidney-tonifying and yang-strengthening traditional Chinese medicine preparation is lack of accuracy in raw material proportioning and fuzzy in medicinal part, so that a preparation effect system is unstable, and the requirements of most people with insufficiency of kidney yang cannot be met are solved.

The invention discloses a method for characterizing volatile components in a traditional Chinese medicine compound containing herba epimedii based on a gas chromatography-mass spectrometry technology. The method comprises the following steps: (1) preparing a traditional Chinese medicine compound test solution containing herba epimedii; and (2) detecting the volatile components in the traditional Chinese medicine compound test solution by applying a gas chromatograph-triple quadrupole-tandem mass spectrometer to the test solution. The gas chromatography-mass spectrometry technology provides an effective way for quality evaluation and quality control of the traditional Chinese medicine compound containing the herba epimedii, and has important guiding significance for development of the traditional Chinese medicine compound containing the herba epimedii.

This invention relates to the field of traditional Chinese medicine preparation technology, and discloses a Qingfu Chaijin granule, its preparation method, and its application. The Qingfu Chaijin granule comprises the following traditional Chinese medicine raw materials in parts by weight: Bupleurum chinense 8-12 parts, Cyperus rotundus 8-12 parts, Citrus reticulata peel 8-12 parts, Salvia miltiorrhiza 12-18 parts, Curcuma longa 8-12 parts, Angelica sinensis 8-12 parts, Ligusticum chuanxiong 5-8 parts, Foeniculum vulgare 8-12 parts, Amethyst 25-35 parts, Artemisia argyi 5-8 parts, Cistanche deserticola 8-12 parts, Epimedium brevicornu 8-12 parts, and Rehmannia glutinosa 8-12 parts. The invention also discloses the application of the aforementioned Qingfuchaijin granules in the preparation of drugs for relieving or treating dysmenorrhea caused by qi stagnation and blood stasis. By scientifically optimizing the compatibility ratio of Qingfuchaijin granules and combining it with a reasonable preparation process design, the resulting Qingfuchaijin granules have been significantly improved in terms of stability, user experience, and performance. The use of dry granulation or spray drying-dry granulation combined technology effectively reduces the initial moisture content of the granules, significantly reduces the hygroscopicity of the granules, and improves the storage stability of the product.

The invention provides a daphne giraldii seed composite treatment solution with epimedium polysaccharide as a main effective component and application, and belongs to the technical field of plant seed treatment, the daphne giraldii seed composite treatment solution comprises 100-300 mg / L of gibberellin, 0.1-100 mg / L of epimedium polysaccharide, 15-35 mg / L of 6-benzylamino adenine, 50-150 mg / L of oligosaccharide, 1-30 mg / L of benzothiadiazole, 20-60 mg / L of a bactericide and 0-1 mL / L of a surfactant. According to the application, the daphne giraldii seeds are treated with the daphne giraldii seed composite treating fluid. According to the composite treatment liquid and the treatment method, the germination rate and the germination potential of the seeds can be remarkably improved, mildewing in the stratification period is effectively inhibited, the growth potential of seedlings is enhanced, a standardized and repeatable operation process is provided, and the composite treatment liquid and the treatment method are suitable for large-scale production.

The invention relates to the fields of environmental protection, fire protection and new materials, in particular to a nano-silver self-assembled efficient disinfectant synthesized by a water extract of epimedium stems. The method comprises the following steps: weighing 10 g of epimedium stem powder, putting the epimedium stem powder into a beaker, then adding 100 mL of purified water into the beaker, putting the beaker on an electrothermal furnace, heating until boiling, then heating for 30 min, after a solid-liquid mixture is cooled, filtering impurities by using gauze, centrifuging for 10 min at a speed of 4000 r / min, retaining supernate, and fixing the volume to 100 mL; 0.17 g of silver nitrate powder is weighed and added into a beaker, then 100 mL of purified water is added for dissolution, and a silver nitrate solution is prepared; adding 20mL of the water extracting solution of the epimedium stems after constant volume into the beaker; mixing the solution, and stirring for 2 hours by using a magnetic stirrer; and the temperature is 60 DEG C to obtain the herba epimedii water extract-nano-silver self-assembled solution.

The invention provides a compound traditional Chinese medicine bag for enhancing kidney meridian and a device and method based on warm stimulation and synergistic point penetration administration. The compound traditional Chinese medicine bag comprises the following components: 5-8 parts of herba epimedii, 3-5 parts of ginseng, 3-5 parts of cistanche, 4-8 parts of rhizoma polygonati, 3-5 parts of cornu cervi pantotrichum, 3-8 parts of moxa and 3-6 parts of ligusticum wallichii. The device comprises a flexible pad cover and a compound traditional Chinese medicine bag, a containing cavity is formed in the flexible pad cover, a temperature control system is arranged in the containing cavity, and bandages are arranged on the two sides of the flexible pad cover. The compound traditional Chinese medicine bag is in close contact with the Taixi acupoint and the Kunlun acupoint through the bandage and the flexible pad outer sleeve, the temperature of the compound traditional Chinese medicine bag is controlled through the temperature control system, the Taixi acupoint and the Kunlun acupoint are accurately stimulated, kidney tonifying and refreshing medicine components are synchronously released, and the synergistic effect of acupoint stimulation and point penetration administration is achieved. Through clinical verification, the method has remarkable advantages in the aspects of improving the sub-health state, boosting vigor and improving the working efficiency.

The invention provides a traditional Chinese medicine composition for nourishing liver and kidney and benefiting essence and blood as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine compositions. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 1.5 to 2.5 parts of oyster peptide, 1.5 to 2.5 parts of sea cucumber peptide, 25 to 35 parts of herba cistanche, 15 to 25 parts of rhizoma polygonati, 10 to 20 parts of fructus lycii, 8 to 12 parts of cortex eucommiae, 8 to 12 parts of fructus rubi, 10 to 20 parts of radix astragali seu hedysari, 20 to 30 parts of herba epimedii, 4 to 6 parts of radix panacis quinquefolii, 1.5 to 2.5 parts of penis et testis cervi, 8 to 12 parts of flos lonicerae, 5 to 8 parts of silkworm chrysalis, 15 to 20 parts of fructus mori, 4 to 6 parts of herba taraxaci, 5 to 8 parts of eurycoma longifolia and 1.5 to 2.5 parts of carpinacol. The traditional Chinese medicine composition disclosed by the invention is reasonable in compatibility, has the effects of tonifying kidney, strengthening essence and nourishing liver, and can be used for treating sexual hypofunction syndromes such as insufficiency of kidney yang, impotence, spermatorrhea and premature ejaculation. The whole prescription is scientific in compatibility, all medicines are mutually coordinated, and both symptoms and root causes are treated.

The invention discloses a traditional Chinese medicine external application preparation for sciatica and femoral head injury and auxiliary treatment equipment. Taking the following materials: tonics: 10g of red ginseng, 10g of codonopsis pilosula, 10g of rhizoma polygonati, 10g of liquorice, 10g of angelica sinensis, 10g of turtle glue, 10g of radix rehmanniae, 10g of dogwood, 10g of cornu cervi pantotrichum, 10g of cistanche, 10g of morinda officinalis, 10g of herba epimedii, 10g of fructus psoraleae, 10g of semen cuscutae, 10g of eucommia ulmoides, 10g of teasel root, 10g of rhizoma drynariae, 10g of fructus alpiniae oxyphyllae, 10g of radix rehmanniae, 10g of turtle glue, 10g of rhizoma anemarrhenae, 10g of dogwood, 10g of parasitic The invention relates to the technical field of traditional Chinese medicine preparations, and has the beneficial effects that through the synergistic effect of compound optimization and intelligent equipment, the comprehensive treatment effects of efficient transdermal absorption, precise targeted treatment, safety and no side effect are realized, and the core problems of poor permeability, single curative effect, insufficient adaptability and the like of a traditional external treatment method are solved; and a safe, efficient and convenient solution is provided for sciatica and femoral head injury.

The invention relates to the technical field of medicinal liquor preparation, in particular to medicinal liquor for treating gynecological diseases, which is prepared from the following raw materials in parts by weight: 1000g of herba epimedii, 100g of tender cornu cervi pantotrichum, 500g of Dong'e donkey-hide gelatin, 150g of female silkworm moth, 120g of actinolitum brevifolium, 200ml of honey and 20kg of 50-degree white spirit. The formula is reasonable, all the raw materials have a synergistic effect, the medicinal liquor product is provided for relieving related gynecological symptoms, the preparation process is simple, complex equipment is not needed, the raw materials are easy to obtain, the medicinal liquor is suitable for home making or small-scale production, use is convenient, the drinking dosage is definite, the taste is milder due to addition of honey, and the medicinal liquor is easy to accept; effective components in the raw materials can be effectively dissolved out, the components can be fully dissolved out through a sealed soaking mode, and the use effect of the medicinal liquor is improved.

A new cultivar of Epimedium plant named ‘Little Bo Peep’ characterized by its compact dense habit and semi-evergreen tri-parted foliage with ovate leaflets that develop as light chartreuse with bronze-burgundy blush in the cooler spring and become medium green in the summer. Medium-sized flowers appear on panicles above the foliage starting in late spring with spurs having a light pinkish-purple tips and medium rose-colored centers, and pinkish-purple sepals. ‘Little Bo Peep’ is useful in the garden landscape as a specimen, en mass, or in containers.

The invention discloses a flavonoid enantiomer compound extracted from Epimedium sagittatum and its preparation method and application. The preparation method comprises the following steps: crushing Epimedium sagittatum to prepare a paste, suspending the paste with water, extracting the paste with petroleum ether, dichloromethane, ethyl acetate and n-butanol respectively to obtain extracts of each part, performing gradient elution separation by silica gel column chromatography, combining similar parts according to the color development results of thin layer chromatography (TLC) to obtain multiple polar segments; chromatographing the target polar segment and performing gradient elution to obtain multiple components, and then combining similar components by thin layer chromatography (TLC) spot plate absorption to obtain multiple sub-components; eluting and purifying the target sub-components, collecting fractions with different retention times, and drying to obtain four compounds respectively. The four compounds have a significant inhibitory effect on the cell viability of human breast cancer MCF-7 cells and have anti-breast cancer activity. The four compounds can be used in the preparation of drugs for treating breast cancer, with huge social and economic benefits.

The invention relates to the technical field of pharmaceutical preparations, in particular to a traditional Chinese medicine composition for treating knee osteoarthritis, a preparation as well as a preparation method and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following medicinal materials in parts by weight: 10 to 15 parts of herba epimedii, 10 to 15 parts of fructus corni, 15 to 25 parts of rhizoma dioscoreae nipponicae, 15 to 30 parts of rhizoma smilacis glabrae, 10 to 15 parts of radix gentianae macrophyllae, 3 to 6 parts of ground beeltle, 10 to 15 parts of spina gleditsiae, 10 to 15 parts of radix achyranthis bidentatae, 10 to 15 parts of cortex eucommiae, 3 to 6 parts of radix notoginseng, 10 to 15 parts of rhizoma drynariae, 10 to 15 parts of radix paeoniae alba, 15 to 30 parts of semen coicis and 3 to 6 parts of liquorice root. The traditional Chinese medicine compound provided by the invention integrates tonifying, blood activating, dampness eliminating and collateral dredging medicines, and avoids the limitation of pure tonifying or evil eliminating, so that the traditional Chinese medicine compound is more in line with the characteristics of KOA deficiency in root and excess in superficies. The traditional Chinese medicine composition is especially suitable for patients with complex knee osteoarthritis with symptoms such as deficiency of liver and kidney, rheumatic arthralgia and stagnation of blood stasis. In addition, through multi-target treatment, the formula gives consideration to both symptoms and root causes, not only aims at local symptoms of KOA, but also adjusts the overall function.

A preparation method of the wine comprises the following steps: 1) weighing the following raw materials in parts by weight: 18-22 parts of raw radix astragali, 13-17 parts of rhizoma drynariae, 13-17 parts of dog bone, 1-5 parts of semen strychni, 1 part of ginseng, 13-17 parts of herba epimedii, 13-17 parts of caulis spatholobi, 3-7 parts of gecko, 1 part of radix salviae miltiorrhizae, 3-7 parts of rhizoma corydalis, 1-5 parts of asarum heterotropoides, 1 part of gynostemma pentaphylla and 3-7 parts of chili; and 2) processing the raw materials into powder, placing the powder in a pottery clay jar, soaking the powder in 50-degree grain white spirit for more than half a year, performing high-temperature fermentation in summer, leaching the liquid medicine, and removing medicine residues to obtain the finished product of the fermented wine for rheumatic bone impediment. The traditional Chinese medicine has the advantages that the traditional Chinese medicine is reasonable in raw material design and convenient to prepare, has a good effect on treating various arthritis such as rheumatism, ankylosing spondylitis, rheumatic arthritis, lumbar disc herniation and hyperostosis, and has a very good popularization prospect and a very good medical economic value.

The invention discloses a preparation for improving male erectile function and sperm quality. The health-care food is characterized by being prepared from the following raw materials in parts by weight: 30-50 parts of herba epimedii extract, 20-40 parts of herba cistanche extract, 15-30 parts of fructus lycii extract, 18-35 parts of semen cuscutae extract, 10-25 parts of herba cynomorii extract, 8-20 parts of maca powder, 6-18 parts of raspberry extract, 0.5-2 parts of lycopene, 1-3 parts of L-carnitine and 0.3-1 part of vitamin B family (vitamin B1, vitamin B2, vitamin B6 and vitamin B12 are mixed according to the mass ratio of 1: 1: 2: 0.5). The herba epimedii extract is selected as a main component, the content of icariin is regulated to be not lower than 60%, the high-content icariin can more effectively promote sex hormone secretion, enhance sexual desire and improve erectile function, the total content of echinacoside and verbascoside in the herba cistanche extract is not lower than 45%, and the herba epimedii extract and the herba cistanche extract have the effect of promoting sex hormone secretion, enhancing sexual desire and improving erectile function. The two components have the effects of tonifying kidney yang and benefiting essence and blood, and can enhance kidney functions.

The invention belongs to the field of new traditional Chinese medicine development, and particularly relates to a traditional Chinese medicine composition composed of herba epimedii, uncaria, radix scrophulariae, rhizoma alismatis and radix cyathulae, an extraction method of the traditional Chinese medicine composition and application of the traditional Chinese medicine composition to treatment of blood pressure circadian rhythm disorder of hypertension. The pharmaceutical composition comprises the following raw material medicines: herba epimedii, uncaria, radix scrophulariae, rhizoma alismatis and radix cyathulae. The traditional Chinese medicine composition provided by the invention is suitable for hypertension disease blood pressure circadian rhythm disorder. According to the pathogenesis characteristics of hypertension, the invention provides the novel traditional Chinese medicine composition for treating blood pressure circadian rhythm disorder of hypertension and the preparation method thereof, and the compatibility effect of mutual promotion and mutual assistance is exerted according to the compatibility principle of monarch, minister, assistant and guide of traditional Chinese herbal medicines. The traditional Chinese medicine composition disclosed by the invention can effectively reduce systolic pressure and diastolic pressure of a hypertensive patient in 24 hours and control morning and night hypertension, and is high in safety. The traditional Chinese medicine composition provides a theoretical and clinical basis and a novel prescription strategy for clinically preventing and treating blood pressure circadian rhythm disorder of hypertension in traditional Chinese medicine.

The invention discloses a traditional Chinese medicine composition for treating sarcopenia by regulating a CXCR4 / GPX4 axis to inhibit ferroptosis and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 5-15 parts of eucommia ulmoides, 5-15 parts of herba epimedii, 5-15 parts of fructus psoraleae and 5-15 parts of semen cuscutae. The traditional Chinese medicine composition disclosed by the invention has the traditional effects of tonifying kidney, strengthening yang and strengthening tendons and bones, and is proved to be capable of promoting GPX4 expression and inhibiting ferroptosis of muscle cells by up-regulating CXCR4 expression, so that muscle strength is effectively enhanced, proliferation and differentiation of muscle satellite cells are promoted, oxidative stress is reduced, and the traditional Chinese medicine composition is used for preventing or treating sarcopenia.

The invention relates to the technical field of traditional Chinese medicine, in particular to a traditional Chinese medicine composition for treating interstitial lung disease with fibrosis and a preparation method and application thereof.The traditional Chinese medicine composition is prepared from, by weight, 5-15 parts of ginseng, 8-15 parts of radix asparagi, 10-20 parts of radix rehmanniae recen, 15-25 parts of astragalus membranaceus, 5-15 parts of schisandra chinensis, 15-25 parts of honey-fried radix asteris, 15-25 parts of honey-fried flos farfarae, 8-15 parts of curcuma zedoary, 8-15 parts of peach kernels and 8-15 parts of herba epimedii. , 15-30 parts of semen lepidii, 10-20 parts of fructus liquidambaris, and 10-20 parts of semen sinapis. The traditional Chinese medicine composition provided by the invention has the effects of strengthening vitality, reducing phlegm, activating blood, removing blood stasis and dredging collaterals, and can improve clinical symptoms of patients with interstitial lung diseases and fibrosis, improve life quality and delay disease progression.

The invention discloses a method for determining total flavonoids in herba epimedii formula granules and application of the method. The method comprises the following steps: acquiring a characteristic chromatogram of a herba epimedii formula granule test solution; collecting peak areas of chromatographic peaks in the characteristic chromatogram, and summing; the chromatographic peaks comprise a peak 8 and a peak 5; calculating the content of total flavonoids according to a mathematical model Y = aX + b, wherein X is the sum of peak areas, Y is the total flavonoid content, a is larger than or equal to 0.90 and smaller than or equal to 1.20, and b is larger than or equal to 10.0 and smaller than or equal to 11.0. According to the method, the specific chromatogram of the herba epimedii formula granules is qualitatively determined, meanwhile, the content of the total flavonoids in the herba epimedii formula granules can be quantitatively determined by determining the sum of the peak areas of several representative chromatographic peaks in the specific chromatogram, a new method is provided for quality control of the herba epimedii formula granules, and the method is high in efficiency and low in cost.

This invention relates to the field of plant tissue culture and the breeding of superior varieties of traditional Chinese medicinal materials. This invention discloses a method for rapid propagation of Epimedium inflorescences via tissue culture, comprising the following steps: S1, collecting newly sprouted flower inflorescences of Epimedium in early spring, disinfecting them, and setting them aside; S2, cutting the disinfected inflorescences into 1.1-1.2 cm segments, inoculating them into callus induction medium, and culturing them under conditions of 1200-1300 lux light intensity, 12 h / day photoperiod, and 24℃. This invention ensures uniform seedling quality and stable traits through targeted screening of effective components and monitoring of genetic stability. The optimized culture medium ratios at each stage result in a high proliferation coefficient, robust rooting, and a short propagation cycle, enabling efficient year-round propagation. Standardized seedling hardening and transplanting lead to a high survival rate. This method is technically stable and highly applicable, enabling the mass production of high-flavonoid glycoside, genetically stable, high-quality seedlings, fundamentally solving problems such as germplasm degradation, low propagation coefficient, and uneven quality, providing strong support for the breeding and industrialization of superior varieties of Epimedium.

The invention discloses a traditional Chinese medicine composition for treating obesity phlegm-dampness obstructing spleen syndrome. Comprising the following raw material medicines in parts by weight: 18-25 parts of astragalus membranaceus, 11-15 parts of bighead atractylodes rhizome (fried), 11-15 parts of poria cocos, 8-12 parts of rhizoma alismatis, 3-5 parts of coptis chinensis (fried with wine), 4-6 parts of rheum officinale (fried with wine), 8-12 parts of semen cassiae (fried), 7-12 parts of radix bupleuri (fried with vinegar), 11-15 parts of hyperforin perforatum, 13-18 parts of oriental wormwood, 10-15 parts of salvia miltiorrhiza (fried with wine), 7-9 parts of curcuma zedoary (fried with vinegar), 7-10 parts of polygonum cuspidatum, 14-20 parts of herba epimedii (fried), 6-8 parts of rhizoma zingiberis and 10-15 parts of glossy privet fruit (steamed with wine). The traditional Chinese medicine composition is prepared from 14-20 parts of prepared rehmannia roots, 18-25 parts of hawthorn fruits, 10-15 parts of lotus leaves, 8-12 parts of mulberry twigs, 7-9 parts of baikal skullcap roots (roasted with wine), 8-12 parts of polygala roots, 10-15 parts of kudzuvine roots and 7-9 parts of licorice roots (roasted). The traditional Chinese medicine composition is researched and developed on the basis of complex pathogenesis and pathogenesis of the obesity phlegm-dampness obstructing spleen syndrome, and has an expected effect of treating the obesity phlegm-dampness obstructing spleen syndrome.

Isoprenyl flavonoid compounds extracted from Epimedium sagittatum and preparation method and application thereof, the preparation method is: crushing Epimedium sagittatum to paste, suspending with water, extracting with petroleum ether, dichloromethane, ethyl acetate and n-butanol respectively to obtain various parts of extract, using silica gel column chromatography to separate by gradient elution, according to the TLC coloration result, similar parts are combined to obtain multiple polarity sections; chromatograph the target polarity section, gradient elution, obtain multiple components, then combine similar components by TLC point plate absorption to obtain multiple component groups; elute and purify the target component group, collect the flow fraction with different retention time, dry, and obtain two compounds respectively, which have significant inhibitory effect on the cell viability of human breast cancer cell MCF-7, have anti-breast cancer activity, and can be applied to prepare breast cancer treatment drugs, with huge social and economic benefits.