586 results about "Epimedium elatum" patented technology

The invention belongs to the field of medicines, and particularly relates to a traditional Chinese medicine compound for treating chronic obstructive pulmonary disease in a stable phase and application thereof. The traditional Chinese medicine composition is prepared from herba epimedii, rhizoma curculiginis, radix astragali, radix salviae miltiorrhizae, lumbricus, rhizoma dioscoreae nipponicae, radix ophiopogonis, radix scutellariae, radix adenophorae, rhizoma zingiberis, cortex mori radicis, rhizoma pinelliae, rhizoma coptidis and semen ginkgo, can play the main effects of tonifying lung and kidney, reducing phlegm and removing blood stasis, and is applied to the stable phase of the chronic obstructive pulmonary disease; the traditional Chinese medicine composition has a good clinical application effect in treatment of chronic obstructive pulmonary diseases, especially lung-kidney qi deficiency syndrome and phlegm-blood stasis syndrome.

The invention provides a male product delay spray and a preparation process thereof, and relates to the field of delay sprays. The preparation process of the male product delayed spray comprises the following steps: S1, preparing water and ethanol, and uniformly stirring and mixing; s2, extracting fructus cnidii, herba epimedii, flos caryophylli, pericarpium zanthoxyli, herba cistanche, herba cynomorii and piper nigrum respectively; s3, obtaining a corresponding extract according to the step S2; s4, putting the obtained extract into water and an ethanol dissolving solution; s5, increasing the stirring speed, heating, and fully dissolving and mixing; s6, stopping stirring and cooling to room temperature; and S7, performing filling. According to the invention, the plant extract is adopted as an effective component, and the special pepper kava extract is selected, so that the irritation can be obviously reduced, the delay effect can be obviously improved, and the effects of reducing the skin sensitivity of the penis and improving the sexual intercourse time can be achieved by applying the plant extract to the skin surface of the male penis.

The present invention relates to the technical field of new drug indications, and specifically discloses a non-filling breast augmentation external skin-rejuvenating oil preparation and its preparation method and application. The preparation method of the external skin-rejuvenating oil preparation is as follows: (1) preparing a skin-rejuvenating oil alcohol extract freeze-dried powder: taking astragalus, angelica, epimedium, ginkgo, green peel, frankincense, rhubarb, chicken gizzard, scutellaria, coptis, phellodendron and borneol in a certain weight ratio and adding them together into a container, and then adding an ethanol solution with a volume fraction of 40%-70% to the container, soaking, flash extraction, filtrate concentration, freeze drying to obtain; (2) preparing a skin-rejuvenating oil preparation: dissolving the skin-rejuvenating oil alcohol extract freeze-dried powder and egg yolk lecithin in edible vegetable oil to obtain an oil phase; then dripping the oil phase into the water phase obtained by dissolving Tween 60 and glycerol in ultrapure water, stirring and high-speed shearing, high-pressure homogenization, and obtaining. The external skin-rejuvenating oil preparation of the present invention has the effect of promoting breast fullness and uprightness in a short period of time.

The invention discloses a traditional Chinese medicine composition for treating constipation and a preparation method thereof, the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10-20g of radix aconiti lateralis preparata, 5-15g of fructus evodiae, 20-30g of herba epimedii, 35-45g of radix astragali, 10-20g of ginseng, 5-15g of rhizoma atractylodis macrocephalae, 20-35g of semen coicis, 50-60g of fructus aurantii immaturus, 10-20g of betel nut and 5-15g of honey-fried licorice root. Comprising the following steps: (1) accurately weighing the raw materials in parts by weight, respectively pulverizing, sieving with a 200-mesh sieve, and mixing to obtain mixed medicinal powder for later use; and 2) adding the mixed medicine powder into a full-automatic traditional Chinese medicine watered pill machine, adding cooked millet accounting for 1-5% of the total amount of the mixed medicine powder, starting the full-automatic traditional Chinese medicine watered pill machine, and processing to obtain watered pills.

The invention relates to the technical field of disease monitoring, in particular to a disease monitoring method and system in the epimedium growth process, and the method comprises the steps: deploying a multi-source collection device in an epimedium planting area, and obtaining plant images in real time according to a preset time interval, the plant images including RGB images and multispectral images; performing image preprocessing on the acquired RGB image to obtain a standard plant image; acquiring plant growth time, performing morphological analysis on the standard plant image to extract growth characteristic parameters, and inputting the growth characteristic parameters and the growth time into a pre-constructed plant growth stage prediction model to acquire the epimedium growth stage. According to the disease monitoring method for the epimedium growth process provided by the invention, by fusing RGB and multispectral images, accurately extracting key features such as diseased leaves and areas, colors and shapes of the diseased leaves, and inputting a disease prediction model in a growth stage, intelligent discrimination of epimedium disease types and severity levels can be realized.

The invention discloses an icaritin carbamate prodrug as well as a preparation method and application thereof, belongs to the field of pharmacy, and particularly relates to an icaritin carbamate prodrug as shown in a formula 3-N or 7-N and pharmaceutically acceptable salts thereof as well as a preparation method and application of the icaritin carbamate prodrug. Specifically, a main metabolic site (3-site or 7-site hydroxyl) of a natural flavone compound icaritin is subjected to structural modification through a chemical synthesis method, and an appropriate group is introduced into the site to form the icaritin carbamate prodrug. Amines adopted by the carbamate are aliphatic amines. Due to existence of a carbamate structure, II-phase metabolism of icaritin in SD rats is reduced, compared with icaritin raw material medicine, the 3-N-01 prodrug has the advantages that oral bioavailability is remarkably improved, the 3-N-01 prodrug is the optimal prodrug, clinical medication is expected to be capable of reducing administration dosage and improving patient compliance, and the 3-N-01 prodrug can be applied to preparation of the SD rats. And an effective strategy is expected to be provided for improving the oral bioavailability of phenolic drugs. # imgabs0 #

The invention relates to the technical field of pharmaceutical analysis, and particularly provides a characteristic chromatogram of epimedium villosum, a characteristic chromatogram construction method and an identification method. According to the method, an epimedium villosum medicinal material is pretreated, and the characteristic chromatogram of the epimedium villosum is identified by means of a chromatographic analysis technology; the epimedium villosum and the planted epimedium villosum are distinguished through the relative peak area and the characteristic peak of the chromatographic peak, so that the technical problem that the wild epimedium villosum and the planted epimedium villosum cannot be distinguished by the existing methods such as appearance identification, thin-layer identification and DNA identification is solved, and the correct source of the wild epimedium villosum is effectively ensured; the method has the advantages of simple identification method and accurate result.

The invention provides a soilless culture high-yield planting method for herba epimedii, and belongs to the technical field of plant planting. The method comprises the following steps that under the condition that illumination is smaller than or equal to 20000 lux, a soilless culture substrate is used for artificially planting herba epimedii, according to the substrate humidity requirement and the root growth condition of the herba epimedii, an irrigation scheme is set, the feed-discharge ratio in different growth periods is adjusted, meanwhile, the water and fertilizer formula proportion is adjusted by combining detection of the plant nutrient content and detection of the feed-discharge ratio, and then the herba epimedii is planted. The survival rate and the technical yield of epimedium are improved. Compared with the prior art, the method has the advantages that the survival rate can be increased by 20%, the seedling recovery time is shortened by more than 15 days, the number of branches can be increased by 30% and the size of leaves can be increased by 20% within the same time, and the yield is increased by 50% from a field to an object cultivation technology by integrating the superior yields.

The invention discloses a traditional Chinese medicine composition for treating triple-negative breast cancer pulmonary metastasis, which is prepared from the following raw material medicines in parts by weight: 3-5 parts of herba epimedii, 3-5 parts of pseudobulbus cremastrae seu pleiones, 5-15 parts of rhizoma curcumae, 2-8 parts of nidus vespae and 5-15 parts of fructus akebiae. The traditional Chinese medicine composition disclosed by the invention has the effects of removing blood stasis and removing stasis, soothing liver and regulating qi, tonifying kidney and strengthening bone, and removing stasis and detoxifying, and can improve the disease-free survival rate of a metastatic breast cancer patient after operation for 2 years, delay the recurrence and metastasis of the postoperative breast cancer, enhance the immunity of the patient and improve the clinical symptoms of the patient. In addition, the invention further discloses a preparation method and application of the traditional Chinese medicine composition.

The invention discloses an anti-aging herba epimedii extract. The anti-aging herba epimedii extract is prepared by performing microbial fermentation on herba epimedii powder. The invention further discloses a preparation method of the anti-aging herba epimedii extract and application of the anti-aging herba epimedii extract in preparation of anti-aging drugs, food and health care products. According to the invention, through special plant lactobacillus and a fermentation process, efficient conversion of main glycoside components in herba epimedii is realized, the contents of icariin and epimedin C are reduced, the contents of secondary glycoside I and secondary glycoside II are remarkably increased, and new icaritin is generated. The herba epimedii extract disclosed by the invention has the effects of resisting aging and prolonging the service life.

The preparation method comprises the following steps: crushing epimedium sagittatum to prepare paste, suspending with water, extracting with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol respectively to obtain extractum of each part, carrying out gradient elution separation by using silica gel column chromatography, and concentrating to obtain the isopentenyl flavone compound extracted from epimedium sagittatum, thereby obtaining the isopentenyl flavone compound extracted from epimedium sagittatum. Combining the similar parts according to a thin layer chromatography (TLC) color development result to obtain a plurality of polar segments; carrying out chromatography and gradient elution on the target polar section to obtain a plurality of components, and then combining the similar components through thin-layer chromatography (TLC) dot plate absorption to obtain a plurality of sub-components; a target component is eluted and purified, fractions with different retention times are collected and dried, and two compounds are respectively obtained, have a remarkable inhibition effect on the cell viability of human breast cancer cells MCF-7, have anti-breast cancer activity, can be applied to preparation of drugs for treating breast cancer, and have huge social and economic benefits.

The invention discloses a traditional Chinese medicine compound for preventing or treating osteoporosis and a preparation method, and relates to the field of traditional Chinese medicine, and the traditional Chinese medicine compound is prepared from the following raw materials in parts by weight: 20-30 parts of salted eucommia ulmoides, 15-20 parts of roasted herba epimedii, 15-20 parts of rhizoma drynariae, 15-30 parts of roasted astragalus membranaceus, 12-20 parts of angelica sinensis, 12-20 parts of fructus lycii and 6-9 parts of liquorice. The traditional Chinese medicine compound has a good effect of treating osteoporosis.

The invention belongs to the field of veterinary drugs, and particularly relates to a medicine for improving immunity of bred animals. The medicine provided by the invention comprises the following active ingredients in parts by weight: 10-20 parts of withered grass 37 peptide and extracts of the following traditional Chinese medicinal materials: 15-30 parts of wintercreeper, 15-30 parts of astragalus membranaceus, 15-30 parts of spirulina, 10-20 parts of silybum marianum and 10-20 parts of herba epimedii. Wherein the withered grass 37 peptide is a biological active peptide with an anti-infection function, is composed of a plurality of amino acids and has broad-spectrum antibacterial activity. The medicine provided by the invention has the functions of inhibiting and sterilizing, regulating immunity and regulating body functions so as to improve the immunity of bred animals, thereby turning pathogenesis and ensuring the health of the bred animals. A curative effect experiment shows that the medicine can improve the growth performance of bred animals and effectively reduce breeding loss, has the advantages of being environmentally friendly, safe and capable of preventing diseases, and has a good market prospect.

The invention relates to the technical field of biology, in particular to an SSR molecular marker primer group for identifying the variety of herba epimedii, a kit and application. The core primers of the SSR molecular marker disclosed by the invention comprise 823, 840, 841, 842, 854 and 866, and the primers can be used for identifying the herba epimedii arrow leaf No.1, the herba epimedii arrow leaf No.2 and the herba epimedii soft hair No.1. The SSR molecule can be used for researching the genetic diversity, genetic relationship and genetic distance of epimedium, and respectively establishing molecular identity cards of three new epimedium varieties, namely epimedium arrow leaf No.1, epimedium arrow leaf No.2 and epimedium soft hair No.1, so that a foundation is laid for genetic map construction, excellent character marking and the like; meanwhile, a scientific basis is provided for constructing a molecular breeding system of a new variety (line) of herba epimedii in the later period.

The invention discloses a traditional Chinese medicine composition for treating male asthenospermia and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine compositions. The traditional Chinese medicine composition comprises prepared rehmannia root, semen cuscutae, raspberry, fructus lycii, schisandra chinensis, semen plantaginis, astragalus membranaceus, Chinese yam, cistanche, herba epimedii and deer-horn glue, the traditional Chinese medicine composition is decocted into decoction or added into boiled honey to be prepared into pills, and the medicine for treating male asthenospermia is obtained. The composition has the advantages of small and precise dosage, small side effect, high safety and low cost. Experimental results show that the traditional Chinese medicine composition can be effectively applied to treatment of male asthenozoospermia due to male kidney essence deficiency, the total effective rate reaches up to 91.67% or above, and the traditional Chinese medicine composition has obvious advantages in improvement of clinical traditional Chinese medicine symptoms compared with western medicines. The invention provides a novel pharmaceutical composition for treating male asthenospermia, and the pharmaceutical composition has practical clinical significance and popularization value.

The preparation method of the flavone enantiomer compound comprises the following steps: crushing epimedium sagittatum to prepare paste, suspending with water, extracting with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol respectively to obtain extractum of each part, carrying out gradient elution separation by using silica gel column chromatography, and concentrating to obtain the flavone enantiomer compound extracted from epimedium sagittatum. Combining the similar parts according to a thin layer chromatography (TLC) color development result to obtain a plurality of polar segments; carrying out chromatography and gradient elution on the target polar section to obtain a plurality of components, and then combining the similar components through thin-layer chromatography (TLC) dot plate absorption to obtain a plurality of sub-components; a target component is eluted and purified, fractions with different retention times are collected and dried, and four compounds are obtained respectively, have a remarkable inhibition effect on the cell viability of human breast cancer cells MCF-7, have anti-breast cancer activity, can be applied to preparation of drugs for treating breast cancer, and have huge social and economic benefits.

The invention relates to the technical field of functional food production through microbial fermentation, in particular to a herba epimedii enzymolysis probiotic fermentation refined extract and a preparation method thereof.The preparation method comprises the following steps that herba epimedii and beta-cyclodextrin are mixed, an obtained mixed solution is subjected to enzymolysis treatment through pectinase, cellulase and glycosidase and then serves as a solid-state fermentation substrate, and the herba epimedii enzymolysis probiotic fermentation refined extract is obtained; adding mixed bacteria of lactobacillus casei, bifidobacterium lactis and lactobacillus gasseri, performing solid-state fermentation, and performing vacuum freeze drying to obtain a herba epimedii probiotic fermentation refined extract; the herba epimedii probiotic fermentation refined extract is prepared by adopting an enzymolysis fermentation method in cooperation with a beta-cyclodextrin inclusion technology, the extraction efficiency, the solubility, the biological activity, the stability and other performance of effective components in herba epimedii are improved, the preparation method is simple, and operation is easy and convenient; the used materials are edible, green and safe; the process saves energy consumption, has good environmental benefits, greatly saves manpower and material resources, and can be applied to industrial large-scale production.

The preparation method comprises the following steps: (1) soaking male silkworm moths in white spirit, extracting, and filtering to obtain a male silkworm moth extracting solution; (2) performing percolation extraction on herba epimedii, semen cuscutae, fructus lycii, cordyceps sinensis, herba cistanche, rhodiola rosea, radix astragali, radix rehmanniae praeparata and stigma croci with white spirit to obtain percolate; (3) combining the male silkworm moth extracting solution and the percolating solution to obtain a medicine extracting solution; and (4) filtering the medicine extracting solution by using a 0.22-1 mu m polypropylene filter membrane, standing for 30-60 days, then filtering by using a 0.22-1 mu m polyethersulfone filter membrane, mixing with rock candy liquid, adjusting the alcohol content to (39 + / -2) degrees, and filtering by using a 0.22-1 mu m polyethersulfone filter membrane to obtain the traditional Chinese medicine wine. The medicinal liquor prepared by the preparation method disclosed by the invention is high in stability, and the problem that precipitates are generated by an original production process is effectively solved.

The preparation method of the isopentenyl flavone enantiomer compound comprises the following steps: crushing epimedium sagittatum to prepare paste, suspending with water, respectively extracting with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol to obtain extractum of each part, carrying out gradient elution separation by using silica gel column chromatography, and concentrating to obtain the isopentenyl flavone enantiomer compound, namely the isopentenyl flavone enantiomer compound. Combining the similar parts according to a thin layer chromatography (TLC) color development result to obtain a plurality of polar segments; carrying out chromatography and gradient elution on the target polar section to obtain a plurality of components, and then combining the similar components through thin-layer chromatography (TLC) dot plate absorption to obtain a plurality of sub-components; a target component is eluted and purified, fractions with different retention times are collected and dried, and four compounds are obtained respectively, have a remarkable inhibition effect on the cell viability of human breast cancer cells MCF-7, have anti-breast cancer activity, can be applied to preparation of drugs for treating breast cancer, and have huge social and economic benefits.

The invention relates to the technical field of traditional Chinese medicine effective component extraction, and provides an efficient extraction and purification process and application of icariin in plateau herba epimedii, the efficient extraction and purification process of icariin in plateau herba epimedii comprises the following steps: S1, epimedium leaves are crushed and sieved to obtain epimedium powder; s2, adding an ethanol solution into the herba epimedii powder, then adding a compound enzyme, performing ultrasonic treatment, inactivating the enzyme, performing suction filtration, and collecting filtrate to obtain an icariin crude extract; s3, diluting the crude icariin extract, adjusting the pH value to 5-6, carrying out chromatographic purification and elution, and collecting the eluate; s4, performing vacuum concentration on the eluent, and performing spray drying to obtain a pure icariin product; the compound enzyme comprises cellulase, pectinase and alpha-mannosidase in a mass ratio of 3: 1: (0.2-1.0). By means of the technical scheme, the problem that in the prior art, the extraction rate of icariin in plateau herba epimedii is low is solved.

The invention relates to a method for extracting two tocopherol derivatives A and B from epimedium sagittatum and application of the two tocopherol derivatives A and B, which can effectively solve the problem of preparing the tocopherol derivatives A and B from the epimedium sagittatum and realizing the application of the tocopherol derivatives A and B in preparing anti-breast tumor drugs, and the method comprises the following steps: crushing the overground part of the epimedium sagittatum, carrying out reflux extraction with ethanol, carrying out reduced pressure recovery to obtain an extract, extracting, and carrying out chromatographic separation to obtain the two tocopherol derivatives A and B; and carrying out gradient elution, reduced-pressure recovery, thin-layer point plate detection, merging, purification, collection of fractions within retention time, and drying to obtain the product. The raw materials are rich, the preparation method is easy to operate, the obtained product is good in quality and good in use effect, the pharmaceutical composition can be effectively used for treating breast cancer, application in preparation of anti-breast cancer drugs is achieved, the medicinal value and commercial value of epimedium sagittatum are developed, and the pharmaceutical composition has remarkable economic and social benefits.

The invention provides a traditional Chinese medicine composition with the effects of warming kidney, supporting yang, promoting blood circulation and relieving pain. The traditional Chinese medicine composition is prepared from the following components: agaric, ligusticum wallichii, radix paeoniae rubra, radix achyranthis bidentatae, dragon's blood, cassia twig, eucommia ulmoides (charcoal), herba pyrolae, radix puerariae, rhizoma corydalis, oyster, radix salviae miltiorrhizae, herba epimedii, fructus psoraleae, trogopterus dung and honey-fried licorice root. The radix salviae miltiorrhizae, the herba epimedii, the fructus psoraleae and the trogopterus dung are added in the formula, so that the effects of nourishing blood, promoting blood circulation, dispersing blood stasis, relieving pain, strengthening muscles and bones, warming and invigorating kidney yang, and promoting blood circulation to relieve pain are improved, and the drug effect is remarkably improved by warming and invigorating, removing blood stasis, promoting blood circulation and relieving pain and blending the medicines with the honey-fried licorice root. Pharmacodynamic experiments prove that the traditional Chinese medicine composition prepared by the invention is remarkable in efficacy, high in bioavailability and stable and reliable in quality, can be prepared into different dosage forms according to the requirements of patients, and is convenient to carry and easy to take.

The invention discloses a method for improving the quality of epimedium seeds and application thereof, and the method comprises the following steps: S1, carrying out fixed-plant and fixed-point observation in a homogeneous garden, recording and observing the flowering habit and synchronism of epimedium delavayi, including the flowering period time and pollination insect species, and observing the flower characteristics; stigma pollination is determined through pollen activity detection, and a basis is provided for subsequent artificial isolated pollination; s2, performing artificial isolation pollination in the flowering phase of the epimedium villosum plants, and using an isolation closing device to prevent cross-pollination. According to the method for improving the quality of the herba epimedii seeds and the application of the method, the flowering rule of the herba epimedii is determined through homogeneous garden transplanting experiments, the pollen viability is dynamically monitored in combination with a TTC staining method, an artificial isolation pollination device and an operation process are designed, and the quality of the herba epimedii seeds is improved. The method has the following technical effects that the seed purity is improved, wherein the seed purity after artificial isolation pollination reaches 90% or above, and the genetic background consistency is remarkably improved.

The invention discloses a preparation method of granules for treating breast and thyroid nodules and canceration, which comprises the following steps: S1, raw material preparation: preparing 199g of codonopsis pilosula, 332g of astragalus membranaceus, 149g of glossy privet fruit, 100g of herba epimedii, 166g of selfheal, 100g of uniflower swisscentaury root, 100g of rhizoma pinellinae praeparata, 100g of curcuma zedoary, 199g of salvia chinensis, 332g of selaginella doederleinii hieron, 100g of curcuma aromatica and 149g of cortex albiziae according to a mass ratio; s2, adding water to decoct the twelve medicines twice, adding 8 times by mass of water for the first time, extracting for 2.0 hours, and filtering; s3, adding 6 times by mass of water for the second time, extracting for 1.5 hours, filtering, and combining filtrates; s4, concentrating under reduced pressure to obtain clear paste with the relative density of about 1.05-1.10 (60 DEG C); and S5, performing vacuum drying, crushing into fine powder, adding dextrin and 10g of stevioside, uniformly mixing, granulating with 75% by volume of ethanol, drying, preparing into 1000g, and packaging, thereby obtaining the product.

The invention relates to the field of health care products, and particularly discloses a composition containing active peptides and application of the composition in improving male functions. The composition is prepared from 0.5 to 1.0 part of oyster peptide, 1.8 to 2.8 parts of ginseng peptide, 0.5 to 1.0 part of fructus perillae peptide, 1 to 3 parts of cordyceps militaris peptide, 2 to 4 parts of penis et testis cervi peptide, 0.5 to 1.5 parts of cordyceps sinensis extract, 3 to 7 parts of total flavonoids of herba epimedii, 1 to 3 parts of cycloastragenol, 1 to 3 parts of fructus lycii extract, 2 to 6 parts of herba dendrobii extract, 1.5 to 2.5 parts of herba cynomorii extract and 0.05 to 0.15 part of glycine. The composition disclosed by the invention has the effects of tonifying kidney qi, nourishing essence and blood, tonifying Yang, tonifying spleen and replenishing qi, strengthening and consolidating body resistance, resisting fatigue and the like, can also improve the sperm motility and improve the reproductive injury condition of an organism, and has positive influence on the fertility and sexual function of males.

The invention relates to the technical field of traditional Chinese medicines, and discloses a traditional Chinese medicine composition for resisting stem cell aging as well as a preparation method and application thereof. The traditional Chinese medicine composition comprises 30 parts of astragalus membranaceus, 15 parts of fructus psoraleae, 15 parts of codonopsis pilosula, 15 parts of roasted rhizoma atractylodis macrocephalae, 10 parts of eucommia ulmoides, 15 parts of herba epimedii, 15 parts of glossy privet fruit, 15 parts of Chinese yam, 15 parts of radix paeoniae alba, 15 parts of gynostemma pentaphylla, 6 parts of pseudo-ginseng powder, 20 parts of sculellaria barbata, 15 parts of poria cocos, 10 parts of radix bupleuri, 30 parts of raw oyster shell, 15 parts of vinegar turtle shell, 15 parts of rhizoma pinellinae praeparata, 15 parts of eclipta, 6 parts of pericarpium citri reticulatae and 6 parts of liquorice. The preparation method comprises water extraction and concentration to prepare powder. The composition can be used for inhibiting the aging of the mesenchymal stem cells and improving the cell activity, and can be combined with the mesenchymal stem cells to treat degenerative bone diseases including osteoporosis and femoral head necrosis.

A method for extracting flavonoid compounds from epimedium sagittatum comprises the following steps: crushing epimedium sagittatum, carrying out reflux extraction, carrying out reduced pressure recovery to obtain an extract, suspending with distilled water, and extracting with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol at room temperature to respectively obtain each part; the dichloromethane part is separated, eluted and combined, and components Fr.A, Fr.B, Fr.C, Fr.D, Fr.E, Fr.F, Fr.G and Fr.H are obtained; eluting the component Fr.G, and combining to obtain components Fr.G1,..., Fr.G17 and Fr.G18; eluting the component Fr.G17, and combining the eluted component Fr.G17 to obtain components Fr.G 17.1,..., Fr.G 17.15,..., Fr.G 17.19; eluting a component Fr.G 17.15, recovering under reduced pressure, and merging to obtain components Fr.G 17.15. 1,..., Fr.G 17.15. 9, Fr.G 17.15. 10,..., Fr.G 17.15. 12; the preparation method comprises the following steps: carrying out semi-preparative HPLC (High Performance Liquid Chromatography) purification on the component Fr.G 17.15. 10 to obtain a compound Epimesatine P; the preparation method comprises the following steps: carrying out semi-preparative HPLC (High Performance Liquid Chromatography) purification on a component Fr.G 17.15. 9, and collecting components with different retention times, so as to respectively obtain the compounds Epimesatine Q, Epimesatine R and Epimesatine S. The preparation method comprises the following steps of: preparing the compound Fr.G 17.15. 9; the flavonoid compound can be effectively extracted from epimedium sagittatum, can be effectively used for treating breast cancer, can be applied to preparation of anti-breast cancer drugs, and has remarkable economic and social benefits.

The invention discloses a radix astragali, rhizoma polygonati and ligustrum lucidum ait traditional Chinese medicine preparation for treating myelosuppression as well as a preparation method and a detection method thereof. The preparation is prepared from 50-150 parts of radix astragali, 50-150 parts of wine-processed rhizoma polygonati, 25-75 parts of wine-processed ligustrum lucidum ait, 50-150 parts of blanched rhizoma drynariae, 50-150 parts of radix trichosanthis and 25-75 parts of herba epimedii. The optimal extraction process and refining process are screened out based on effective components and pharmacological activity through a large number of tests, and the optimal preparation process of the Shuanghuangliangwhitening oral liquid and other traditional Chinese medicine preparations is obtained through stability investigation and verification. According to the invention, a thin layer chromatography is adopted, the four medicinal materials of astragalus membranaceus, glossy privet fruit, rhizoma drynariae and herba epimedii are respectively subjected to thin layer chromatography research, and methodology research shows that the method is stable and feasible. The invention establishes a content determination method for astragaloside, calycosin-7-glucoside, specnuezhenide and icariin, establishes a quality standard, and has important significance for ensuring the clinical curative effect and safety of astragaloside, calycosin-7-glucoside, specnuezhenide and icariin.

The invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to a kidney-tonifying and liver-nourishing compound preparation and a preparation method thereof. The compound preparation comprises 5-15 parts of roasted herba epimedii, 5-15 parts of fructus cnidii, 50-70 parts of roasted radix astragali and 10-30 parts of pulp of dogwood fruit. The traditional Chinese medicine composition has remarkable curative effects on various diseases and chronic strain caused by deficiency of liver and kidney and deficiency of blood and essence, such as dizziness, blurred vision, fatigue, swelling and pain of waist, back and limbs, dry mouth and eyes, limb ache and weakness, irregular menstruation, incapability of holding weight or hemiplegia and the like, and has a very good application prospect.

The invention relates to the technical field of herba epimedii planting, in particular to an optimized herba epimedii planting method adjusted by utilizing environmental characteristics, which comprises the following steps: summarizing physiological characteristics, required soil conditions, greenhouse environment, fertilizer requirement rules and management characteristics of herba epimedii, and uploading the summarized physiological characteristics, required soil conditions, greenhouse environment, fertilizer requirement rules and management characteristics to a control system; selecting a planting base, and detecting and analyzing a planting environment index of planting soil; preparation before planting, wherein mature herba epimedii seeds are stored in sand; a shade shed is built on the planting base, and water and fertilizer integrated drip irrigation equipment and an environment monitoring system are arranged in the shade shed; seedling raising and sowing are conducted, the composition of planting soil is adjusted according to the planting environment index of the planting base, and a proper planting environment is established through water and fertilizer integrated drip irrigation equipment and an environment monitoring system; the herba epimedii seeds stored in the sand are sown on the seedbed; transplanting is conducted; and field management. According to the herba epimedii optimized planting method adjusted through the environmental characteristics, healthy growth of herba epimedii is guaranteed, and the quality of herba epimedii is improved.