Konjak in vitro rapid breeding method
A konjac potato and fast technology is applied in the field of rapid in vitro propagation of konjac potato, can solve the problems of weak ability, plant toxicity, large interference, etc., and achieves the effects of increasing the population number, overcoming variation, and slowing the reproduction speed.
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[0033] 1. Introduction
[0034] In December 1996, 5 konjac potato plants from Yunnan Xishuangbanna Tropical Botanical Garden were introduced from Yunnan Province. The leaves were cut to leave rhizomes and roots brought back. They were placed in a shade shelter with a shading degree of 85% and planted in pond mud: peat soil. : Coarse sand=6:3:1 in the matrix, keep the relative humidity of the air at 90%.
[0035] 2. Obtaining sterile explants
[0036] The aseptic seedlings of seeds can be obtained through indoor and outdoor aseptic sowing:
[0037] Seeds were collected from 1997 to 2000 for germination experiments. The berries were harvested and sown in August each year after they had matured (based on their dryness on the pavilion), and the germination rate was counted 60 days after sowing.
[0038] Indoor sowing method: immediately rinse the seeds from the mother plant with tap water, treat with 70% alcohol for 12s, rinse with sterile water for 5 times, then disinfect with 0.1% m...
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