Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Biosynthesis method for improving yield of epothilone B

A technology for epothilone and biosynthesis is applied in the biosynthesis of industrialized production to improve the yield of epothilone B, and in the field of biosynthesis of epothilone, it can solve the problem of high production cost and low yield of epothilone B and other problems to achieve the effect of shortening the production cycle, increasing growth and increasing productivity

Active Publication Date: 2012-07-18
HUBEI HONCH PHARMA
View PDF1 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem of high production cost and low yield of epothilone B in the prior art, and provide a kind of epothilone with high yield, which can adapt to industrial production and can effectively reduce production cost. Biosynthetic method, the yield of epothilone B in the active product produced by the expansion and fermentation of this method can reach as high as 30-45%, which has a good industrial production prospect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biosynthesis method for improving yield of epothilone B
  • Biosynthesis method for improving yield of epothilone B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: a kind of biosynthetic method of improving the productive rate of epothilone B of the present invention, the synthesis step comprises:

[0032] (1), produce starting bacterial classification, select the strong and vigorous starting myxobacterial bacterial classification as production bacterial classification by 3000rpm centrifugal shake flask bacterial liquid;

[0033] (2), solid slant expansion culture: its slant medium components are calculated by mass%, glucose 0.5%, peptone 0.5%, starch 1%, sodium chloride 0.5%, gibberellin 0.002% and agar powder 2%, The balance is water; sterilize at 120°C for 30 minutes, and after 24 hours, spread the well-grown production starting strains on a solid slant medium, and cultivate them at 30°C±2°C and relative humidity of 55% for 8 days;

[0034] (3) Inoculate the well-growing strains into the shake flask expansion medium sterilized at 120°C for 30 minutes. The components of the shake flask expansion medium are starch 5...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a biosynthesis method for improving yield of epothilone B. The biosynthesis method comprises the following steps of: selecting robust strains for producing the epothilone B by using a centrifugal process, inoculating the strains into a sterilized seed medium, performing shaking culture at 30DEG C on a shaker, and fermenting; adding a precursor inductor phenylalanine into a fermentation medium; harvesting a culture solution after culturing at 30DEG C for 10 to 11 days; and collecting, adsorbing and saturating by using macroporous resin, eluting by using ethyl acetate, separating and purifying, performing vacuum concentration, extracting, crystalizing at low temperature, and performing vacuum drying. The purity of the epothilone B can be effectively improved by a liquid preparation column, and is over 99.5 percent; and methanol is adopted for extraction and separation, so that the epothilone B is crystallized in the methanol, and the finished product reaches thepharmaceutical grade. By the method, the yield of the epothilone B is greatly improved and is 30-45 percent, and the method has a good industrial production prospect.

Description

technical field [0001] The invention relates to a biosynthesis method of epothilones, in particular to a biosynthesis method for increasing the yield of epothilone B in industrialized production, and belongs to the field of fermentation and cultivation of antibiotic microorganisms. Background technique [0002] Epothilone B is a new member of macrolide antibiotics, and epothilone is a class of antibiotics with unique structure and unique biological activity. Its molecular structure is as follows: [0003] [0004] Epothilone A [0005] Epothilone B [0006] Among them, epothilone B is a macrolide compound produced by myxobacteria (Pyracystis cellulosus). Epothilone B not only has anti-tumor effects, but also has good antagonistic and therapeutic effects on skin diseases caused by fungal infections. Epothilone B has low toxicity and few side effects, and has good application prospects. However, the current method for preparing epothilone B by fermentation has high produ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P17/18C07D493/04C12R1/01
Inventor 陈庆源何福彪张文凯
Owner HUBEI HONCH PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products