The invention relates to a zooplankton sampling device capable of convenient observation and belongs to the field of marine exploration and development. The device comprises an upper cover plate, a frame, a first wall surface, a rotating door, a rotating disk, a second wall surface, a third wall surface, a fourth wall surface, a biological storage bucket, a pressing plate, a pressurization motor,a temperature control device, a rotating motor, a lower cover plate, a base, a bearing, and a handle, wherein the lower end of the frame is clamped in the rotating disk; the hand grips the handle to rotate the frame to enable the frame to rotate along with the rotating disk, so that biological samples can be observed in all directions; a manipulator of a submersible clamps the whole sampling device without touching the biological samples, so that the biological samples can not be damaged, and subsequent experimental studies are facilitated; and soft films are attached to the rotating door, thebiological storage bucket, the first wall surface, the second wall surface, the third wall surface and the fourth wall surface, so that the biological samples can be in soft contact with the samplingdevice, and the mechanical damage of the biological samples is avoided. While the biological samples are collected, seawater in sampling environments is retained, and the subsequent studies are facilitated.