Biological storage agent for edible fungi, preparation method of biological storage agent and edible fungi preservative paper

An edible fungus and storage agent technology, applied in the fields of food preservation, food science, fruit and vegetable preservation, etc., can solve the problems of incomplete removal of bacterial cells, reduce metabolic activity, affect edible value, etc., and achieve increased adsorption and binding capacity, Prolonged shelf life and easy handling

Active Publication Date: 2014-03-26
河北绿茵生化科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent CN200910209787.1 (application date October 29, 2009) discloses a biological preservative for peaches. Although it contains metabolites of Bacillus subtilis and Tween, it is directly sprayed on the surface of peaches for preservation, but the The patented method cannot completely remove the bacterial cells only by centrifuging at 4500 rpm for 30 min, so there is still a risk of direct use of Bacillus subtilis and its spores
The second is that edible fungi belong to the respiratory climacteric type. After picking, they still carry out strong metabolic activities and respiration, which can easily lead to elongation, discoloration, softening, and corruption of the stipe, which affects the edible value. When storing, attention should be paid to reducing its own metabolic activities.
However, its application in the field of biological preservation has not been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Obtaining extracellular metabolites of Bacillus subtilis BSD-2:

[0039] (1) Activation of strains: Activate the preserved strains on the culture medium plate, and pick single colonies to multiply them for later use.

[0040] (2) Fermentation broth preparation: prepare starch bean cake powder medium according to the conventional method, insert the activated BSD-2 strain in step (1) after sterilization, shaker speed 200 rpm, temperature (30±1) ℃, culture for 2~ On day 3, the metabolites of BSD-2 were secreted in the extracellular medium during the culture process.

[0041](3) Separation of metabolites: centrifuge the liquid culture medium in step (2) to remove the part of Bacillus subtilis and solid matter in the culture medium, and filter it with a microporous membrane (0.22 μm), the obtained filtrate contains metabolites product.

[0042] (4) Take 1 g of the obtained filtrate containing metabolites and dilute it with 99 grams of water so that the mass percentage of t...

Embodiment 2

[0045] Preparation of edible mushroom storage agent.

[0046] Bacillus subtilis BSD-2 metabolite 3%, add water to supplement to 100%.

Embodiment 3

[0048] Preparation of edible mushroom storage agent.

[0049] (1) Bacillus subtilis BSD-2 metabolites 1%, starch 3%, chitosan 1.5%, the rest is supplemented by water to 100%, and placed in a stirred reactor.

[0050] (2) Stir at 500 rpm for 30-50 min at 80 °C until evenly stirred.

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PUM

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Abstract

The invention relates to a biological storage agent for edible fungi, and in particular relates to the biological storage agent for edible fungi, a preparation method of the biological storage agent and a piece of edible fungi preservative paper. The biological storage agent comprises extracellular metabolite containing bacillus subtilis BSD-2 which is preserved in Chinese Common Microbe Bacterial Preservation Administration Center with the preservation No. of CGMCC No.8440, and the preservative paper with excellent preservation effect is developed. The biological storage agent can be applied to storage of the edible fungi, so as to obviously reduce brown stain of the edible fungi, and prolong the preservation time for 8-15 days at low temperature. The biological storage agent is free from other chemical components and pollution, is environment-friendly and safe, and further suitable for postharvest preservation and freshness of other vegetables and fruit, and can be widely popularized and applied.

Description

technical field [0001] The invention relates to a biological storage agent for edible fungi, in particular to a biological storage agent for edible fungi containing extracellular metabolites of Bacillus subtilis, belonging to the technical field of food storage. Background technique [0002] In recent years, the production of edible fungi in my country has developed rapidly, and it has become a major producer and exporter of edible fungi in the world. Edible fungus products have become one of the main commodities for exporting agricultural and sideline products in my country. Freshly picked edible mushroom bodies are rich in nutrients and are highly susceptible to infection by microorganisms, causing physiological diseases of the mushroom body and deterioration and corruption. In addition, it is easy to cause damage during storage and transportation, causing discoloration, deterioration or rot, which cannot meet the requirements of sales and processing, and brings huge econo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/155
Inventor 程辉彩张丽萍张根伟董玉兰李书生尹淑丽习彦花崔冠慧张飞燕段普凡
Owner 河北绿茵生化科技有限公司
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