17 results about "Extracellular metabolite" patented technology

The invention discloses Mucor abundans and application of the Mucor abundans, the strain number of the Mucor abundans is JSAFC 2484, the Mucor abundans is preserved in the China General Microbiological Culture Collection Center on May 6, 2025, and the preservation number is CGMCC No. 41979. The Mucor abundans can be applied to the field of biological engineering. The biocontrol bacterium Mucor abundans JSAFC 2484 provided by the invention can effectively inhibit the growth of Fusarium equiseti, the inhibition rate of plate confrontation culture reaches 68.52%, meanwhile, extracellular metabolites secreted by the biocontrol bacterium Mucor abundans JSAFC 2484 can also significantly reduce the growth of the Fusarium equiseti, when the concentration of the extracellular metabolites of the JSAFC 2484 is 10 mL / 100 mL, the inhibition rate of the Fusarium equiseti reaches 76.35%, and when the concentration of the extracellular metabolites of the JSAFC 2484 is 100 mL / 100 mL, the inhibition rate of the Fusarium equiseti reaches 79.05%. Meanwhile, the plant growth promoting effect is achieved on pepper plants.

The invention discloses mucor oosperm and application of the mucor oosperm, the bacterial strain number of the mucor oosperm is JSAFC 2483, the mucor oosperm is preserved in the China General Microbiological Culture Collection Center on May 6, 2025, and the preservation number is CGMCC No.41980. The mucor oosperm has the advantages that the mucor oosperm can be used for preparing the mucor oosperm; the biocontrol bacterium Mucor moelleni JSAFC 2483 provided by the invention can effectively inhibit the growth of Fusarium equiseti, the inhibition rate of plate confrontation culture reaches 54.90%, meanwhile, extracellular metabolites secreted by the biocontrol bacterium Mucor moelleni JSAFC 2483 can also significantly reduce the growth of the Fusarium equiseti, when the concentration of the extracellular metabolites of the JSAFC 2483 is 10 mL / 100 mL, the inhibition rate of the Fusarium equiseti reaches 53.98%, and when the concentration of the extracellular metabolites of the JSAFC 2483 is 100 mL / 100 mL, the inhibition rate of the Fusarium equiseti reaches 56.64%. Meanwhile, the plant growth promoting effect is achieved on pepper plants.

The invention belongs to the technical field of microorganisms, discloses a strain of pseudomonas putida and application of the strain of pseudomonas putida as fish probiotics, and particularly discloses a strain of pseudomonas putida. The invention provides a symbiotic pseudomonas putida CD separated from fish, and the strain has relatively strong fat utilization capability, can generate a plurality of beneficial extracellular metabolites, can promote metabolism of fish lipids, carbohydrates and proteins and weight gain, and also can improve the iron content and hemoglobin content in fish tissues. The pseudomonas putida strain CD disclosed by the invention has good biological safety, and has a wide development prospect in the aspect of application as fish probiotics.

The invention discloses a bacillus safensis strain and application thereof in aquaculture, and relates to the technical field of aquaculture disease prevention and control, the obtained bacillus safensis strain ZY-1 forms spores under a certain fermentation condition, can be prepared into powder for preservation, has a long shelf life, has the bacteriostatic activity retention rate of more than or equal to 85% after being stored in a dark place for 12 months at 4-25 DEG C, and has the antibacterial activity of more than or equal to 7.2%. The production, transportation and storage cost is saved, main bacteriostatic effect factors of the bacillus safensis ZY-1 strain exist in extracellular metabolites, the bacillus safensis ZY-1 strain is stable in performance and resistant to high temperature, acid and alkali, ultraviolet rays, protease and the like, and fermentation supernatant of the bacillus safensis ZY-1 strain can be directly applied or prepared into spray-dried powder and is suitable for various aquaculture application scenes such as pond splashing and feed mixing and feeding. The metabolite spray-dried powder has a remarkable killing effect on high-pathogenicity vibrios VpAHPND and VpTPD, the minimum inhibitory concentrations of metabolite spray-dried powder respectively reach 23.44-46.88 mu g / ml and 46.88-93.75 mu g / ml, and the metabolite spray-dried powder can be used for preventing and controlling prawn infectious hepatopancreas necrosis and prawn transparent larva diseases induced by the metabolite spray-dried powder and also has a certain killing function on pathogens of other aquatic animals.

This invention provides a method for preparing a high-salinity zinc oxide-resistant nanofluid mediated by Halomonas sp. and its application in enhancing oil recovery. Specifically, it includes: a Halomonas sp. strain CDH-2, with accession number CCTCC NO: M 2025786, which can rapidly grow in high-concentration salt solutions and produce salt-resistant extracellular metabolites. Fermenting and culturing CDH-2 under high-salt conditions yields a fermentation broth with a surface tension below 30 mN / m. Mixing and culturing the obtained fermentation broth with a metal precursor solution yields a high-salinity-resistant metal nanofluid. The resulting metal nanofluid maintains good stable dispersibility in high-salinity water and effectively reduces oil-water interfacial tension. It alters the wettability of oil-wetting surfaces, emulsifies and disperses crude oil, and reduces crude oil viscosity. Therefore, it can be used as an enhanced oil recovery agent in high-salt reservoirs and is widely applied in oilfield flooding, well huff and puff, and heavy oil viscosity reduction.

The application discloses application of Enterobacter kobei in controlling and resisting evolution of soil bacteria antibiotic resistance. Enterobacter kobei LB1 is preserved in the China General Microbiological Culture Collection Center on May 15, 2026, and has a preservation number of CGMCC No. 38684 and a preservation address of No. 1, Xibaixili, Chaoyang District, Beijing, China Institute of Microbiology. Enterobacter kobei LB1 and extracellular metabolites have the ability of controlling and resisting evolution of soil bacteria antibiotic resistance. The application verifies the inhibiting effect of the resistance mutant strain of the control agent through a soil microcosm experiment, realizes effective intervention on the evolution of soil microbial resistance, and has the advantages of green ecology, safety, high efficiency, long-term control and the like, and provides a new technical way for environmental antibiotic resistance pollution treatment.

The invention relates to the field of microorganisms, and provides a plant lactobacillus FVPGZP2101 and application thereof, the plant lactobacillus FVPGZP2101 is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 2, 2024, and the preservation number is CGMCC No.32871. The invention also provides a preparation method of the plant lactobacillus FVPGZP2101. The new strain of the plant lactobacillus obtained by screening has the advantages of strong growth ability, high acid production efficiency, strong acid resistance, capability of degrading nitrite, tryptamine, tyramine, spermidine and spermine, no generation of biogenic amine, no degradation of pectin, antibacterial activity on pathogenic bacteria, oxidation resistance of extracellular metabolites, and no pollution to the environment. According to the present invention, the new strain has advantages of high yield, key volatile substance production and non-volatile substance production, and the fermented food prepared by using the new strain has characteristics of low salt content, rich lactic acid bacteria content, nutrition, health and unique flavor; a food fermentation process developed by utilizing the new strain disclosed by the invention is high in fermentation efficiency, short in production period and high in application value.

This invention discloses a species of *Mucor* and its applications, wherein the *Mucor* ( Mucor moelleri The strain number, JSAFC 2483, was deposited on May 6, 2025, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 41980. The biocontrol bacteria provided by this invention... Mucor moelleri JSAFC 2483 can effectively inhibit Fusarium equisetifolium ( Fusarium equiseti The growth of *Fusarium equisetifolium* was inhibited by 54.90% in plate confrontation culture, and its secreted extracellular metabolites also significantly reduced the growth of *Fusarium equisetifolium*. When the concentration of JSAFC 2483 extracellular metabolites was 10 mL / 100 mL, it significantly reduced the growth of *Fusarium equisetifolium*. F. equiseti The inhibition rate reached 53.98%. When the extracellular metabolite concentration of JSAFC 2483 was 100 mL / 100 mL, it had a significant inhibitory effect on... F. equiseti The inhibition rate reached 56.64%. At the same time, it promoted the growth of pepper plants.

The application belongs to the technical field of microorganisms, discloses a Pseudomonas putida and application of the Pseudomonas putida as fish probiotics, and specifically discloses a Pseudomonas putida Pseudomonas putida CD, the strain has strong fat utilization capacity, can produce various beneficial extracellular metabolites, can promote fish lipid, carbohydrate and protein metabolism and weight gain, and can also improve the iron content and hemoglobin content in fish tissues. The Pseudomonas putida strain CD has good biological safety and has broad development prospects in the application as fish probiotics.

The invention provides a biological purification method for removing aluminum impurities in a solution by using penicillium fermentation liquor, which is particularly applied to a rare earth leaching solution. The aluminum-resistant penicillium is enriched and screened from rare earth mining area soil, fermentation liquor is prepared and mixed with a rare earth leaching solution according to a certain liquid-liquid ratio to react, aluminum is promoted to be removed from the leaching solution in the forms of complexing / precipitating / adsorbing and the like within 5 hours under the mild conditions of 25-32 DEG C and pH of 3.4-5.5, and meanwhile it is guaranteed that the loss of rare earth elements is smaller than 5%. Organic acids such as oxalic acid and citric acid and extracellular metabolites generated in the fermentation liquor can be subjected to complexation, adsorption or precipitation reaction with Al < 3 + >, so that the aluminum impurities are efficiently removed. The biological purification method for removing the aluminum impurities in the solution by using the penicillium fermentation liquor is simple and convenient to operate, does not need a large number of chemical precipitators, is suitable for removing and purifying the aluminum impurities in the rare earth hydrometallurgy process, and has remarkable economic and environmental benefits.

The present invention cultivates Chlorella and prepares its extracellular metabolites, which are then used to promote plant growth and / or improve plant stress resistance. Chlorella extracellular metabolites promote lateral root elongation and cold resistance of Arabidopsis thaliana and Nicotiana benthamiana. Chlorella extracellular metabolites fermented at high cell density can promote the growth of rapeseed and vegetables such as tomatoes, cucumbers, Chinese cabbage, and lettuce when diluted 500 to 1000 times. Experiments also found that the yield of rapeseed treated with Chlorella extracellular metabolites was increased, and the vitamin C and protein content in the vegetables increased. This shows that Chlorella extracellular metabolites can not only enhance the cold resistance (stress resistance) of vegetables, but also increase the yield and nutritional value of leaf vegetables, providing a new technical research and development direction for the cultivation of high-quality organic vegetables. The present invention also provides a resource processing method for the supernatant after harvesting Chlorella culture and a preparation method for a natural plant stimulant.

The invention discloses an algicidal bacterial agent as well as a preparation method and application thereof, and relates to the technical field of environmental microorganisms and water treatment. The active component of the algicidal bacterial agent comprises extracellular metabolites of bacillus licheniformis. According to the invention, the bacillus licheniformis with algae-lysing capability is obtained through separation. An extracellular metabolite of the strain is a core active component, and experiments prove that the strain has an efficient algae-lysing effect on various common algae such as microcystis aeruginosa, chlorella and coleus elegans, and breaks through the technical bottleneck of narrow algae-lysing spectrum of a single strain. A novel algicidal bacterial agent is developed on the basis of the bacillus licheniformis, the active ingredients of the algicidal bacterial agent are stable in property under conventional storage conditions, and the algicidal bacterial agent has high efficiency and environmental friendliness. The method provides stable and reliable technical support for algae control in a complex water body environment, and has a wide application prospect.

System for the microbial synthesis of bioactive silver nanoparticles for anticancer applications, including: • a microbial culture vessel for cultivating bacterial strains that produce extracellular metabolites; • a separation unit for obtaining a cell-free culture filtrate from the microbial culture; • a reaction chamber for mixing the cell-free filtrate with a silver precursor solution; • a nanoparticle formation module for the production of silver nanoparticles by reduction of silver ions using microbial metabolites in the filtrate; wherein the produced silver nanoparticles exhibit cytotoxic activity against cancer cells.

A method for predicting the metabolic state of a cell culture of cells of a specific cell type includes providing a metabolic model of a cell of the specific cell type, and performing at each of a plurality of points in time during cultivation of the cell culture, receiving measured concentrations of a plurality of extracellular metabolites and a measured cell density in the culture medium; inputting the received measurements as input parameter values to a trained machine learning program logic-MLP; predicting extracellular fluxes of the extracellular metabolites at a future point in time by the MLP; performing metabolic flux analysis to calculate the intracellular fluxes at the future point in time based on the predicted extracellular fluxes and the stoichiometric equations of the metabolic model.

The invention relates to carthamus tinctorius acid and application of carthamus tinctorius acid in antibacterial substances in the technical field of microbial metabolite application, the carthamus tinctorius acid is derived from a co-culture fermentation extracellular metabolite of rosa roxburghii endophytic fungus Epicoccum laussicola GUCC 191049.1 and helminthosporium bombycis, the molecular formula of the carthamus tinctorius acid is C15H20O5, and the carthamus tinctorius acid is shown in the description. According to the scheme, a new natural candidate substance is provided for solving the problems of drug resistance and environmental risk of an existing chemical antibacterial agent.