50 results about "Extracellular metabolite" patented technology

The invention discloses an agrobacterium vitis strain AE206. The strain is preserved in the China General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms, and has a preserving registration number of CGMCC No.2408. The invention also discloses a microbial preparation and a preparation method thereof. The active ingredients of the preparation are AE206 thalli and extracellular metabolite of the AE206 thalli. The agrobacterium vitis AE206 has good prevention and treatment effect on grape crown gall, has wide antimicrobial spectrum, and has good prevention effect on peach crown gall and cherry crown gall. Moreover, the microbial preparation belongs to a biological agent, and has the characteristics of non-pollution, non public nuisance, low cost and so on.

Relating to the field of microorganism, the invention discloses a polypeptide and a lactobacillus plantarum extracellular metabolite, application thereof, a method for inducing lactobacillus plantarum to produce bacteriocin and an identification method. The invention specifically relates to a polypeptide shown as SEQ ID NO:1, a lactobacillus plantarum extracellular metabolite containing the polypeptide and a preparation method, application of the polypeptide and / or lactobacillus plantarum extracellular metabolite in inducing lactobacillus plantarum to produce bacteriocin, a method for inducing lactobacillus plantarum to produce bacteriocin and an identification method of bacteriocin-producing lactobacillus plantarum. The polypeptide and / or lactobacillus plantarum extracellular metabolite can induce lactobacillus plantarum to express bacteriocin, the expression levels of the four genes papA, papB, papC gene and papD related to bacteriocin production in a lactobacillus plantarum gene are respectively increased by 9.41, 6.75, 7.94 and 6.63 times, thus indicating that adding of the polypeptide and / or lactobacillus plantarum extracellular metabolite can induce the expression of related genes in lactobacillus plantarum.

The invention relates to a process which is capable of inducing cucumbers to resist powdery mildew metabolites separated from trichoderma harzianum with lecular modification, which takes advantages of gene integration technique of restriction endonuclease mediated, constructs transformant of trichoderma harzianum by preparing strain protoplast of trichoderma harzianum and extracting plasmid, then the transformant is cultivated and fermented, extracellular metabolites of trichoderma harzianum transformant capable of systematic performance to induce cucumbers to resist powdery mildew are selected from the fermentation liquid. The extracellular metabolites of trichoderma harzianum transformant of the invention can overcome the weakness that living trichoderma harzianum is not stable in induced resistance effect. The invention not only has remarkable control effect to powdery mildew, but also facilitates growth of cucumber seedlings, and provides an efficient process for rapidly and highly efficiently selecting novel microbial pesticide guide compound from trichoderma harzianum for preparation of green molecular pesticide.

Disclosed are hair care compositions containing partially purified extracellular metabolite preparation from strains of Bacillus coagulans. Specifically, the uses of compositions containing extracellular metabolite preparation from a strain of Bacillus coagulans for increasing hair growth, inhibition of 5α-reductase and proliferation of follicle dermal papilla cells and in the management of androgenic alopecia, are disclosed.

The invention discloses a Bacillus subtilis strain HMB20428 for preventing and treating cucumber ampho disease, and the Bacillus subtilis strain HMB20428 is preserved in the China General Microbiological Culture Collection Center on December 20, 2011, and the preserving registration number is CGMCC No.5616. The invention further discloses a microbial agent prepared by the strain and a preparationmethod thereof. The active ingredients of the microbial agent are Bacillus subtilis HMB20428 thalli and extracellular metabolite of the Bacillus subtilis HMB20428 thalli. The strain HMB20428 has strong specificity for the cucumber ampho disease; the control effect is high; and the average control effect can reach above 85%; through the method provided by the invention for preventing and treating the cucumber ampho disease, drug resistance is difficult to generate; the microbial agent is safe to human and livestock, and is free of environment pollution; and the preparation method is simple, the cost is low, and the microbial agent is convenient to use.

The invention discloses a Bacillus pumilus strain HMB22277 for controlling cottonbollblight which is preserved in the China General Microbiological Culture Collection Center on December 20, 2011 with the preservation number of CGMCC NO. 5615. The invention further discloses a microorganism bacterium agent produced by using the microbe and a preparation method thereof. The active component of the microorganism bacterium agent is Bacillus pumilus HMB22277 thalli and its extracellular metabolite. According to the invention, the Bacillus pumilus HMB22277 has strong targeted performance on cottonbollblight with high control effect, wherein the average control effect is higher than 77%; secondly the microorganism bacterium agent of the invention is safe for human and animals and has no environmental pollution and doesn't easily develop drug resistance; in addition in addition, the preparation method of the microorganism bacterium agent is simple, the cost is low and the application is convenient.

The invention discloses an agrobacterium vitis strain AE206. The strain is preserved in the China General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms, and has a preserving registration number of CGMCC No.2408. The invention also discloses a microbial preparation and a preparation method thereof. The active ingredients of the preparation are AE206 thalli and extracellular metabolite of the AE206 thalli. The agrobacterium vitis AE206 has good prevention and treatment effect on grape crown gall, has wide antimicrobial spectrum, and has good prevention effect on peach crown gall and cherry crown gall. Moreover, the microbial preparation belongs to a biological agent, and has the characteristics of non-pollution, non public nuisance, low cost and so on.

The invention relates to a microecological preparation used for preventing and treating apostichopus japonicus putrid skin syndrome and a preparation method of the microecological preparation and belongs to the field of aquatic microorganisms. Active ingredients of the microecological preparation are thalli of bacillus megatherium H1 and extracellular metabolites of the bacillus megatherium H1; the bacillus megatherium strain is separated from intestinal tract excrement of apostichopus japonicus which is healthily cultivated in Penglai, Shandong, detection of safety, bacteriostatic activity and toxicity and artificial antagonistic vibrio splendidus experiments verify that the bacillus megatherium H1 is antagonistic to vibrio splendidus, young apostichopus japonicus is effectively protected, the bacillus megatherium H1 can be used for preventing and treating apostichopus japonicus 'putrid skin syndrome' caused by vibrio splendidus and survival rate of an apostichopus japonicus seedling protecting stage is increased.

Relating to the field of microorganism, the invention discloses a polypeptide and a lactobacillus plantarum extracellular metabolite, application thereof, a method for inducing lactobacillus plantarum to produce bacteriocin and an identification method. The invention specifically relates to a polypeptide shown as SEQ ID NO:1, a lactobacillus plantarum extracellular metabolite containing the polypeptide and a preparation method, application of the polypeptide and / or lactobacillus plantarum extracellular metabolite in inducing lactobacillus plantarum to produce bacteriocin, a method for inducing lactobacillus plantarum to produce bacteriocin and an identification method of bacteriocin-producing lactobacillus plantarum. The polypeptide and / or lactobacillus plantarum extracellular metabolite can induce lactobacillus plantarum to express bacteriocin, the expression levels of the four genes papA, papB, papC gene and papD related to bacteriocin production in a lactobacillus plantarum gene are respectively increased by 9.41, 6.75, 7.94 and 6.63 times, thus indicating that adding of the polypeptide and / or lactobacillus plantarum extracellular metabolite can induce the expression of related genes in lactobacillus plantarum.

Disclosed is the use of partially purified extracellular metabolite isolated from Bacillus coagulans MTCC 5856 to prevent skin aging. More specifically the invention discloses the anti-collagenase, anti-elastase, anti-glycation activity and enhancement of TGF-β, epidermal growth factor and hyaluronic acid expression in human dermal fibroblasts, of extracellular metabolites isolated from Bacillus coagulans MTCC 5856.

The invention provides a Pseudomonas fluorescens XF10 with the preservation number being CGMCC No.13703. The bacterial liquid, the extracellular metabolites and the volatile products of the Pseudomonas fluorescens can effectively inhibit the tobacco black shank. The Pseudomonas fluorescens screened in the invention has high activity and is stable, and the fermentation broth, the extracellular metabolites and the volatile products of the Pseudomonas fluorescens can effectively kill Phytophthora parasitica var nicotianae; a root irrigation manner is adopted, so the operating process is simple; and the Pseudomonas fluorescens has no pollution, and has no harms to the environment.

The invention mainly relates to the technical field of textiles, and discloses a processing method of waste acrylic cloth. The processing method comprises the following steps: sorting, removing impurities, decoloring, sterilizing, melting at a high temperature, reinforcing fibers, spinning and shaping. The method is simple, the obtained renewable acrylic fibers are high in strength, the decoloring rate is up to 85.7 percent, no toxic reagent is contained used in the preparation process, and health and environmental protection can be realized; the waste acrylic cloth is cleaned after being sorted, and impurities are removed, so that the subsequent treatment is facilitated; and the primarily selected cloth is arranged in a decoloring pool, a fermentation solution and zymophyte are added to firstly perform the fermentation at a high temperature and to promote the proliferation of the bacteria, various microorganisms perform the metabolism, a great amount of extracellular metabolites are generated, the combination capacity of the dye and cloth is reduced, the decoloration is facilitated, and then the fermentation solution is replaced to perform the low-temperature fermentation, so that the decoloration effect of the waste acrylic fibers is obviously improved, the application range of the renewable acrylic fibers is enlarged, and the safety and health of the renewable acrylic fibers can be improved.