8 results about "Extracellular metabolite" patented technology

This invention provides a method for preparing a high-salinity zinc oxide-resistant nanofluid mediated by Halomonas sp. and its application in enhancing oil recovery. Specifically, it includes: a Halomonas sp. strain CDH-2, with accession number CCTCC NO: M 2025786, which can rapidly grow in high-concentration salt solutions and produce salt-resistant extracellular metabolites. Fermenting and culturing CDH-2 under high-salt conditions yields a fermentation broth with a surface tension below 30 mN / m. Mixing and culturing the obtained fermentation broth with a metal precursor solution yields a high-salinity-resistant metal nanofluid. The resulting metal nanofluid maintains good stable dispersibility in high-salinity water and effectively reduces oil-water interfacial tension. It alters the wettability of oil-wetting surfaces, emulsifies and disperses crude oil, and reduces crude oil viscosity. Therefore, it can be used as an enhanced oil recovery agent in high-salt reservoirs and is widely applied in oilfield flooding, well huff and puff, and heavy oil viscosity reduction.

The application discloses application of Enterobacter kobei in controlling and resisting evolution of soil bacteria antibiotic resistance. Enterobacter kobei LB1 is preserved in the China General Microbiological Culture Collection Center on May 15, 2026, and has a preservation number of CGMCC No. 38684 and a preservation address of No. 1, Xibaixili, Chaoyang District, Beijing, China Institute of Microbiology. Enterobacter kobei LB1 and extracellular metabolites have the ability of controlling and resisting evolution of soil bacteria antibiotic resistance. The application verifies the inhibiting effect of the resistance mutant strain of the control agent through a soil microcosm experiment, realizes effective intervention on the evolution of soil microbial resistance, and has the advantages of green ecology, safety, high efficiency, long-term control and the like, and provides a new technical way for environmental antibiotic resistance pollution treatment.

This invention discloses a species of *Mucor* and its applications, wherein the *Mucor* ( Mucor moelleri The strain number, JSAFC 2483, was deposited on May 6, 2025, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 41980. The biocontrol bacteria provided by this invention... Mucor moelleri JSAFC 2483 can effectively inhibit Fusarium equisetifolium ( Fusarium equiseti The growth of *Fusarium equisetifolium* was inhibited by 54.90% in plate confrontation culture, and its secreted extracellular metabolites also significantly reduced the growth of *Fusarium equisetifolium*. When the concentration of JSAFC 2483 extracellular metabolites was 10 mL / 100 mL, it significantly reduced the growth of *Fusarium equisetifolium*. F. equiseti The inhibition rate reached 53.98%. When the extracellular metabolite concentration of JSAFC 2483 was 100 mL / 100 mL, it had a significant inhibitory effect on... F. equiseti The inhibition rate reached 56.64%. At the same time, it promoted the growth of pepper plants.

The invention provides a biological purification method for removing aluminum impurities in a solution by using penicillium fermentation liquor, which is particularly applied to a rare earth leaching solution. The aluminum-resistant penicillium is enriched and screened from rare earth mining area soil, fermentation liquor is prepared and mixed with a rare earth leaching solution according to a certain liquid-liquid ratio to react, aluminum is promoted to be removed from the leaching solution in the forms of complexing / precipitating / adsorbing and the like within 5 hours under the mild conditions of 25-32 DEG C and pH of 3.4-5.5, and meanwhile it is guaranteed that the loss of rare earth elements is smaller than 5%. Organic acids such as oxalic acid and citric acid and extracellular metabolites generated in the fermentation liquor can be subjected to complexation, adsorption or precipitation reaction with Al < 3 + >, so that the aluminum impurities are efficiently removed. The biological purification method for removing the aluminum impurities in the solution by using the penicillium fermentation liquor is simple and convenient to operate, does not need a large number of chemical precipitators, is suitable for removing and purifying the aluminum impurities in the rare earth hydrometallurgy process, and has remarkable economic and environmental benefits.

The invention discloses an algicidal bacterial agent as well as a preparation method and application thereof, and relates to the technical field of environmental microorganisms and water treatment. The active component of the algicidal bacterial agent comprises extracellular metabolites of bacillus licheniformis. According to the invention, the bacillus licheniformis with algae-lysing capability is obtained through separation. An extracellular metabolite of the strain is a core active component, and experiments prove that the strain has an efficient algae-lysing effect on various common algae such as microcystis aeruginosa, chlorella and coleus elegans, and breaks through the technical bottleneck of narrow algae-lysing spectrum of a single strain. A novel algicidal bacterial agent is developed on the basis of the bacillus licheniformis, the active ingredients of the algicidal bacterial agent are stable in property under conventional storage conditions, and the algicidal bacterial agent has high efficiency and environmental friendliness. The method provides stable and reliable technical support for algae control in a complex water body environment, and has a wide application prospect.

System for the microbial synthesis of bioactive silver nanoparticles for anticancer applications, including: • a microbial culture vessel for cultivating bacterial strains that produce extracellular metabolites; • a separation unit for obtaining a cell-free culture filtrate from the microbial culture; • a reaction chamber for mixing the cell-free filtrate with a silver precursor solution; • a nanoparticle formation module for the production of silver nanoparticles by reduction of silver ions using microbial metabolites in the filtrate; wherein the produced silver nanoparticles exhibit cytotoxic activity against cancer cells.

A method for predicting the metabolic state of a cell culture of cells of a specific cell type includes providing a metabolic model of a cell of the specific cell type, and performing at each of a plurality of points in time during cultivation of the cell culture, receiving measured concentrations of a plurality of extracellular metabolites and a measured cell density in the culture medium; inputting the received measurements as input parameter values to a trained machine learning program logic-MLP; predicting extracellular fluxes of the extracellular metabolites at a future point in time by the MLP; performing metabolic flux analysis to calculate the intracellular fluxes at the future point in time based on the predicted extracellular fluxes and the stoichiometric equations of the metabolic model.