Soil bacilli for preventing and controlling fruit tree crown gall and inocula thereof and preparation method

A technology of Agrobacterium and microbial inoculum, applied in the field of agricultural microorganisms

Inactive Publication Date: 2009-06-10
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are some non-pathogenic Agrobacterium (Agrobac terium), Pseudomonas (Pseudomonas), Bacillus (Bacillus) and Rahnella (Rahnella) research reports on the prevention and treatment of grape root cancer at home and abroad, but , there is no report on the application in production

Method used

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  • Soil bacilli for preventing and controlling fruit tree crown gall and inocula thereof and preparation method
  • Soil bacilli for preventing and controlling fruit tree crown gall and inocula thereof and preparation method
  • Soil bacilli for preventing and controlling fruit tree crown gall and inocula thereof and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The preparation of AE206 fermented liquid comprises the steps:

[0050] (1) Strain activation: Streak the AE206 strain stored at -20°C on the LB plate medium and culture it at 28°C for 24 hours, then pick a single colony and culture it on the LB slant medium for 24 hours at 28°C ,spare.

[0051] (2) Shake flask cultivation: according to 0.03% by weight, the AE206 bacterial classification obtained in step (1) is placed in culture fluid (the composition and weight percentage of culture fluid are: sucrose 0.8%, peptone 0.5%, beef extract 0.5% , yeast powder 0.1%, MgSO 4 0.05%, the rest is water), cultured on a shaker at 28°C and a pH value of 7.0 for 2 days to obtain AE206 bacterial liquid;

[0052] (3) Place the AE206 bacterium solution obtained in step (1) in the seed culture solution according to 5% volume ratio (the composition and weight percentage of the seed culture solution are: 2.0% sucrose, 0.5% sodium glutamate, 0.5% yeast extract, (NH 4 ) 2 SO 4 0.3%, K...

Embodiment 2

[0055] The preparation of AE206 liquid preparation comprises the following steps:

[0056] Get the AE206 fermented liquid prepared by Example 1 and place it in the container, then add xanthan gum and methylcellulose in the AE206 fermented liquid according to the ratio of xanthan gum 0.3g / L, methylcellulose 0.8g / L, mix Evenly.

Embodiment 3

[0058] The preparation of AE206 solid preparation comprises the following steps:

[0059] The AE206 fermentation broth prepared in Example 1 is mixed with the solid filler according to the ratio of parts by weight of 1:4 (the solid filler is mixed by peat soil, xanthan gum and methylcellulose in the following weight percentages: peat soil 99.4%, xanthan gum 0.3%, methyl cellulose 0.3%) were mixed, then shaded and air-dried to make the water content of the mixture 20%, that is, a solid preparation was made.

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Abstract

The invention discloses an agrobacterium vitis strain AE206. The strain is preserved in the China General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms, and has a preserving registration number of CGMCC No.2408. The invention also discloses a microbial preparation and a preparation method thereof. The active ingredients of the preparation are AE206 thalli and extracellular metabolite of the AE206 thalli. The agrobacterium vitis AE206 has good prevention and treatment effect on grape crown gall, has wide antimicrobial spectrum, and has good prevention effect on peach crown gall and cherry crown gall. Moreover, the microbial preparation belongs to a biological agent, and has the characteristics of non-pollution, non public nuisance, low cost and so on.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to an Agrobacterium for preventing and treating root cancer of fruit trees, a microbial agent produced by the Agrobacterium, a preparation method and an application. Background technique [0002] Root cancer of fruit trees such as grapes, cherries, and peaches occurs to varying degrees in orchards and nurseries in many regions of my country, among which the disease occurs in Liaoning, Inner Mongolia, Beijing, Hebei, Henan, Shandong, Shanxi, Hubei, Zhejiang, and Shanghai. serious. The incidence rate of some nurseries and orchards is above 60%, which seriously affects the quality of seedlings, resulting in a significant reduction in the rate of seedlings and plants. Even if the plants are mature, the growth is weak, and the fruit yield and quality are significantly reduced, with a loss of 30-70%. In severe cases, even the gardens will be destroyed and the harvest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/01
Inventor 李金云王慧敏陶万强王建辉王金利郭岩彬
Owner CHINA AGRI UNIV
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