Bacillus atrophaeus for prevention and control of cotton boll blight, and microbial agent thereof

A technology of Bacillus atrophicus and microbial inoculum, which is applied to the application field of cotton boll blight prevention and control, can solve problems such as few researches on the biological control of cotton boll blight, and achieve the effects of difficult drug resistance, low cost and good control effect.

Active Publication Date: 2012-10-24
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few studies on the biological control of cotton boll blight

Method used

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  • Bacillus atrophaeus for prevention and control of cotton boll blight, and microbial agent thereof
  • Bacillus atrophaeus for prevention and control of cotton boll blight, and microbial agent thereof
  • Bacillus atrophaeus for prevention and control of cotton boll blight, and microbial agent thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation of HMB22922 microbial bacterial agent is carried out according to the following steps:

[0046] (1) Strain activation: The bacterial strain HMB22922 (Bacillus atrophaeus) strain HMB22922 stored at -40°C has been preserved in the General Microorganism Center of the China Committee for the Collection of Microbial Cultures on December 20, 2011, and the preservation number is CGMCC No.5614) is activated (30°C) on LB plate medium (its composition and weight ratio are: tryptone 1g yeast extract 0.5g, sodium chloride 1g, agar powder 15g, water 1000mL), pick Take a single colony on LB slant medium (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar powder 15g, water 1000mL), culture at 30°C for 24-36 hours; the activated bacterial strain was obtained;

[0047] (2) Preparation of seed liquid: make LB liquid medium according to the conventional method (its composition and weight ratio are: tryptone 1g, yeast extract 0....

Embodiment 2

[0050] Example 2 Antagonism Test of Bacillus atrophaeus HMB22922 to Phytophthora phytophthora

[0051] (1) Test time and place: In early August 2010, it was carried out in the Plant Disease Biological Control Laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences.

[0052] (2) Test method:

[0053] (1) Source of cotton boll Phytophthora bacterium: Phytophthora 5-1 was collected from cotton bolls in Hejie Village, Hejie Town, Xianxian County, Cangzhou City, Hebei Province, and was isolated and purified by the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences, Hebei Agricultural University The Plant Pathology Department of the Institute of Plant Protection identified it as Phytophthora boehmeriae, and the pathogenicity test showed strong pathogenicity.

[0054] (2) Tablet confrontation experiment:

[0055] First, Phytophthora 5-1 is activated on the PDA plate, and after 5 days of cultivation, in the e...

Embodiment 3

[0059] Example 3 Comparative Test of Bacillus atrophaeus HMB22922 on the Control of Cotton Boll Blight

[0060] (1) Test drug:

[0061] (1) Microbial agent: the HMB22922 liquid preparation prepared in Example 1 was diluted 50 times with water.

[0062] (2) Chemical agent: aluminum triethylphosphonate wettable powder (80%) (Zhejiang Jiahua Chemical Co., Ltd.), diluted 800 times with water.

[0063] (3) Blank control: clear water.

[0064] (2) The time and place of the experiment: in late August 2010, it was carried out in the artificial climate chamber of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences.

[0065] (3) Test method: collect healthy bolls from the middle and upper parts of cotton plants in the test field (about 30 days after boll formation), require fresh bract leaves, and disinfect the surface with 75% alcohol after bringing them back to the laboratory, and let them dry naturally. Spray respectively on the surface of cotton ...

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Abstract

The present invention discloses a strain of Bacillus atrophaeus HMB22922 for prevention and control of cotton boll blight, wherein the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 20, 2011, and the preservation number is CGMCC No.5614. The present invention further discloses a microbial agent produced by using the strain, and a preparationmethod thereof. Active ingredients of the microbial agent are HMB22922 thallus and extracellular metabolites thereof. The HMB22922 strain of the present invention provides special prevention and control effects for cotton boll blight, wherein the prevention and control efficiency is high, and the average prevention and control efficiency is more than 80%. The microbial agent of the present invention is safe for human and animals, and does not cause environmental pollution. Drug resistance is not easily generated when the strain and the microbial agent are adopted to prevent and control the cotton boll blight. In addition, the preparation method for the microbial agent of the present invention has characteristics of simpleness, low cost, and easy use.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to a bacillus atrophaeus for preventing and treating cotton boll blight, a microbial agent containing the bacillus atrophaeus, and their application in preventing and treating cotton boll blight. Background technique [0002] Cotton boll blight is a serious cotton disease caused by Phytophthora boehmeriae sawada. It not only directly damages cotton bolls, but also provides infection conditions for other pathogens. The methods to prevent and control cotton boll blight are mainly cultivation measures such as pushing plants and ridges, picking rotten bolls early, and chemical agent control. At present, the economic benefits of growing cotton in my country are relatively low, and the labor costs are high. Farmers are unwilling to invest too much energy in pushing the plants together; picking rotten bolls early can only keep part of the yield, which is not enough to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
Inventor 鹿秀云马平李社增郭庆港李宝庆
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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