26 results about "Plant pathology" patented technology

The present application relates to the technical field of plant pathology, and particularly relates to a method for artificially inducing large sporulation of Ascochyta aspidii, comprising the following steps: S1, strain activation: inoculating Ascochyta aspidii into PDA culture medium, and culturing under light at 20 DEG C for 7 days; S2, sporulation induction: inoculating the activated Ascochyta aspidii into an optimized sporulation culture medium, culturing under near-ultraviolet light at 20 DEG C for 14 days, and obtaining a large amount of spores, wherein the components of the optimized sporulation culture medium include 200 mL / L V8 juice, 0.3 g / L CaCO3, 1 g / L glucose, 1 g / L potassium nitrate, and 15-20 g / L agar. The present application establishes a rapid, efficient and simple in-vitro sporulation method of Ascochyta aspidii, which can effectively solve the technical problem of difficult sporulation under conventional culture conditions in a laboratory, and provides sufficient experimental materials for in-depth analysis of the pathogenic mechanism.

The application belongs to the technical field of molecular plant pathology, and particularly relates to a peanut disease-resistant gene AhNHL24 and application thereof. Through whole genome identification and bioinformatics analysis, 45 NHL family genes (AhNHL) are identified in peanuts, and gene expression analysis shows that most of the AhNHL genes can respond to the induction of biological stress and abiotic stress. Gene disease resistance identification finds that transient overexpression of AhNHL14, AhNHL24 and AhNHL31 can significantly improve the resistance of tobacco and peanuts to Pseudomonas solanacearum, and the disease resistance of AhNHL24 is the strongest. Through transgenic tobacco heterologous overexpression of AhNHL24 and inoculation experiments, it is shown that the peanut and tobacco plants overexpressing AhNHL24 not only have significantly enhanced resistance to Pseudomonas solanacearum, but also have significantly enhanced resistance to Sclerotium rofsii. The application provides an important basis for the cultivation of plant (peanut and tobacco, etc.) broad-spectrum disease-resistant varieties.

The invention discloses a common wild rice OrLRR1 gene and application thereof in improving rice blast resistance of rice, and belongs to the technical field of genetic engineering and phytopathology. The nucleotide sequence of the oryza vulgaris OrLRR1 gene is as shown in SEQ ID NO: 1. It is found that the OrLRR1 gene plays an important role in rice blast resistance of rice. The deletion of the OrLRR1 gene reduces the resistance of the rice to the magnaporthe oryzae, and the over-expression of the OrLRR1 gene can improve the resistance of the rice to the magnaporthe oryzae. The invention provides an application of an OrLRR1 gene or an encoding protein thereof in improving the resistance of rice to rice blast, and a method for cultivating rice blast-resistant rice by regulating and controlling the expression of OrLRR1. The invention provides a new gene resource and a theoretical basis for cultivating a new rice variety with high rice blast resistance, and has important application value.

The utility model relates to the technical field of plant culture, and discloses a novel plant pathology culture dish which comprises a first culture dish, a placing plate is arranged on the bottom wall of the first culture dish, connecting plates are fixedly connected to the left side and the right side of the placing plate, and a fixing plate is arranged on the bottom wall of the placing plate. Sliding grooves are formed in the left side and the right side of the fixing plate correspondingly, a rotating button is rotationally connected to the left side of the connecting plate, moving rods are fixedly connected to the upper end and the lower end of the outer wall of the rotating button correspondingly, a ball bearing is fixedly connected to the other end of each moving rod, and the ball bearings are fixedly connected to the left side of the outer wall of the connecting plate. According to the laboratory culture dish, the effect of fixing the placing plate is achieved, the fixing plate cannot deviate and collapse backwards, the fixing rods have the effect of fixing the telescopic rods, and the problem that the utilization rate of the space in the laboratory is low due to the fact that the culture dish occupies more vertical space in the limited space of the laboratory is solved.

The utility model relates to the technical field of plant pathology research, and discloses a pressure-adjustable cassava leaf germ inoculator which comprises a box body and a gun body placed in the box body, the inner wall of the gun body is fixedly connected with a first clamping block and a second clamping block to fix an injector, and the inner wall of the gun body is fixedly connected with a second sliding rail and a second electric push rod. A first sliding rail is fixedly connected to the bottom end of the gun body, a supporting frame is slidably connected into the first sliding rail, the first electric push rod drives the supporting frame to move along the first sliding rail, a pressure sensor and a soft cushion are fixedly connected to the outer wall of the supporting frame, and the soft cushion corresponds to an injection opening of the injector. The second electric push rod is matched with the second sliding rail to achieve automatic quantitative inoculation, the pressure sensor and the soft cushion are matched with the first electric push rod to achieve pressure closed-loop adjustment, cassava leaves are effectively prevented from being damaged, and inoculation efficiency is remarkably improved.

The invention relates to the technical field of biology, in particular to plant agrobacterium tumefaciens, application of the plant agrobacterium tumefaciens, a plant efficient infection preparation and an infection method. The strain of the plant agrobacterium tumefaciens is Agrobacterium tumefaciens MT-1, the strain is preserved in the Guangdong Microbial Culture Collection Center on October 22, 2025, and the preservation number is GDMCC No: 67146. The strain can efficiently infect plants. The agrobacterium tumefaciens MT-1 has a remarkable dip-dyeing effect on plants, particularly Chinese rose plants, can induce formation of cancer tumors in a short time and realizes efficient infection, so that the agrobacterium tumefaciens MT-1 has a good application value in efficient infection of the plants, and has a wide application prospect. A powerful experimental basis is provided for subsequent plant pathology research, related gene research and the like.

The application discloses a banana fusarium wilt secreted protein FoXYG1 and application thereof, and belongs to the technical field of molecular plant pathology. The secreted protein FoXYG1 can induce plant defense response, including causing plant cell hypersensitive necrosis, inducing callose accumulation and up-regulated expression of a plant immune-related pathway marker gene, activating SA, JA, ET and HR pathway-mediated defense pathways to induce plant immune response, and can significantly improve the resistance of plants to fusarium oxysporum and / or fusarium oxysporum. A new way is provided for improving plant disease resistance and reducing disease occurrence, and thus has a wide application prospect in the green development of modern agriculture.

The application belongs to the cross field of agricultural information technology and plant pathology, and provides a detection method for resistance of Chinese rose to powdery mildew based on beta-alanine, which comprises the following steps: step 1, measuring the content of beta-alanine in Chinese rose leaves; step 2, establishing an analysis model between beta-alanine and Chinese rose powdery mildew; and step 3, establishing a resistance grading model to output different levels of resistance; the application can accurately quantify the resistance of Chinese rose to powdery mildew by measuring the content of beta-alanine in Chinese rose leaves and establishing an analysis model between beta-alanine and Chinese rose powdery mildew.

The invention belongs to the technical field of molecular plant pathology, and particularly relates to a peanut disease-resistant gene AhNHL24 and application thereof. Through whole genome identification and bioinformatics analysis, 45 NHL family genes (AhNHL) are totally identified in peanuts, and gene expression analysis shows that most AhNHL genes can respond to induction of biological stress and abiotic stress. The gene disease resistance identification finds that the instantaneous overexpression of AhNHL14, AhNHL24 and AhNHL31 can significantly improve the resistance of tobacco and peanut to ralstonia solanacearum, and the disease resistance of AhNHL24 is the strongest. The transgenic tobacco heterologous overexpression AhNHL24 and inoculation experiments show that not only is the resistance of peanut and tobacco plants for overexpression AhNHL24 obviously enhanced to ralstonia solanacearum, but also the resistance to sclerotium rolfsii is also obviously enhanced. The invention provides an important basis for cultivation of broad-spectrum disease-resistant varieties of plants (peanuts, tobaccos and the like).

The utility model relates to the technical field of plant pathology, and discloses a plant pathogen pathogenic gene target screening device which comprises a screening assembly, and a first fixing assembly used for preventing the screening assembly from shaking is arranged on the upper side of the screening assembly. According to the plant pathogen pathogenic gene target screening device, the conical flask is clamped and fixed through the first fixing assembly and the second fixing assembly, so that the relative position between the conical flask and the fixing table is fixed, the phenomenon that a culture medium is leaked due to displacement, toppling or violent shaking of the conical flask when a shaking table oscillates is avoided, and meanwhile, the screening efficiency is improved. Beneficial bacteria and pathogenic bacteria are completely separated in space, thallus mixing is fundamentally avoided, the premise is created for single strain research, active substances secreted by the beneficial bacteria in the conical flask are continuously and directionally conveyed to the pathogenic bacteria environment on the basis of physical isolation, dynamic intervention is achieved, and the screening efficiency is improved. And a stable and controllable special model is provided for experimental operation.

The invention discloses an anti-streptomycin gene as well as an encoding protein and application thereof, and belongs to the technical field of phytopathology. The invention discloses a novel streptomycin resistance gene aphh (3) derived from tomato canker pathogen. The gene is located on a newly found plasmid pCM3 of tomato canker pathogen, and is used for coding a protein APH (3) belonging to an aminoglycoside antibiotic phosphotransferase family. The functions of the gene are verified through tests of gene knockout, function complementation, heterologous expression and the like, meanwhile, mass spectrometric analysis proves that APH (3) can catalyze streptomycin molecules to generate phosphorylation reaction to generate streptomycin monophosphate, so that the inhibitory activity on bacteria is lost, and a strain carrying the gene has remarkable resistance to streptomycin. The streptomycin resistance gene aphh (3) can be widely applied to the fields of pathogenic bacterium resistance mechanism research, resistance strain screening, molecular marker selection and the like, and has important scientific research and application values.

The invention discloses a magnaporthe oryzae inoculation device and method, and relates to the technical field of plant pathology research instruments.The magnaporthe oryzae inoculation device comprises an inoculation box, the top of the inoculation box is fixedly connected with a water tank used for keeping the humidity of the humid environment in the inoculation box, and two inoculation assemblies used for inoculation are arranged outside the inoculation box; the bottom of the inoculation box is fixedly connected with a control box; and an observation window for observing the growth condition of rice after internal inoculation is arranged on the outer wall of the inoculation box. Through the opposite-direction rotation system of the flow guide plate and the culture tank, the inclined plane design of the flow guide plate and the inclined plane design of the circular baffle and the flow guide plate, uniform coverage of rice leaves by liquid medicine, improvement of inoculation efficiency and liquid medicine utilization rate, integration of light supplement, humidity control and waste liquid separation functions, optimization of experimental conditions and reduction of operation complexity are realized; the growth condition of the rice can be recorded without opening the device through the observation window and the transparent circular baffle, and environmental interference is reduced.

The present application relates to the technical field of plant pathology and agricultural science research, more particularly to a rice seedling blight inducing device and method, wherein the device comprises a box body, a box cover, a first measuring cup and a second measuring cup, the box cover is detachably connected to the top of the box body, the box body is a transparent structure, the box cover is provided with air holes, the bottom of the box body is provided with a drainage hole, the side wall of the box body is provided with a scale, the capacity of the first measuring cup is greater than that of the second measuring cup, and the first measuring cup can be inverted to cover the second measuring cup to form a closed germination cavity. The present application has the advantages of simple structure and facilitating operation standardization; through the sandwich type matrix structure of "bottom moisture layer + seed layer + top covering layer", combined with the two-stage culture mode of indoor constant temperature disease induction and outdoor natural adversity induction, the disease incidence is significantly improved, and disease identification is facilitated.

This invention discloses a pathogen-induced promoter Mig6p and its application in improving disease resistance in rice, belonging to the fields of plant pathology and genetic engineering. This invention screened a rice promoter Mig6p (nucleotide sequence shown in SEQ ID NO.1) induced by multiple pathogens; Mig6p exhibits very low basal expression activity and is not induced by various abiotic stresses. Furthermore, a dominant-negative regulatory mutant of the Mig6p promoter-induced lesion gene Lrd6-6, Lrd6-6, was constructed. E315Q The expression vector was obtained by transforming rice to obtain Mig6p:Lrd6-6 E315Q Rice, verified, Mig6p:Lrd6-6 E315Q The broad-spectrum disease resistance of rice was significantly improved, while agronomic traits were not significantly affected. This invention provides important guidance for improving crop disease resistance using lesion-like genes and also provides a reference for improving other similar traits.

The invention relates to the technical field of agricultural microbiology and phytopathology, in particular to application of rhodopseudomonas palustris in prevention and treatment of rice blast, by spraying a bacterial suspension of the strain with the concentration of 1 * 10 < 8 > CFU / mL to leaf surfaces of rice in the three-leaf one-core stage, a rice linolenic acid metabolic pathway can be regulated and controlled, and the rice blast resistance is improved. The accumulation of an endogenous disease-resistant metabolite 12-oxygen-plant dienoic acid is obviously induced. The OPDA inhibits the magnaporthe oryzae through a dual mechanism, the area of a plate inhibition zone reaches 94.5% + / -2% when the concentration is 50 micrograms / mL, the spore germination inhibition rate reaches 97.3% + / -1.2% (repeating for three times), and a rice jasmonic acid defense system can be activated. The disease-resistant molecular mechanism is defined, the control effect is equivalent to that of chemical pesticides, and a new strategy is provided for developing efficient and stable microbial-source rice blast-resistant preparations.

The invention discloses a simple and rapid rubber tree powdery mildew vacuole observation method, and belongs to the technical field of phytopathology and microbiological microscopic observation. The invention discloses a simple and rapid method for observing vacuoles of powdery mildew of a rubber tree. The vacuoles of powdery mildew are observed by using cytochemical staining and a microscopic technology. The form of the vacuoles treated by the method is not influenced, the form and the number of the vacuoles are easy to microscopically observe, and a foundation is laid for developing invasion of the powdery mildew of the rubber trees, responding to a drought stress mechanism and the functions of the vacuoles; technical support is provided for studying the growth and development state and metabolic activity of the bacterium, regulating the transport process of cell environment substances and the like.

This invention discloses a streptomycin resistance gene, its encoded protein, and its applications, belonging to the field of plant pathology technology. This invention discovers a novel streptomycin resistance gene derived from *Tomato Canker*. aph(3) This gene is located on a newly discovered plasmid pCM3 of *Tomato Canker*, encoding a protein APH(3) belonging to the aminoglycoside antibiotic phosphotransferase family. This invention verified the function of this gene through gene knockout, functional complementation, and heterologous expression experiments. Simultaneously, mass spectrometry analysis demonstrated that APH(3) catalyzes the phosphorylation of streptomycin molecules, generating streptomycin monophosphate, thereby eliminating its inhibitory activity against bacteria. Strains carrying this gene exhibit significant resistance to streptomycin. This invention relates to a streptomycin resistance gene. aph(3) It can be widely used in the study of pathogen resistance mechanisms, screening of resistant strains, and selection of molecular markers, and has important scientific research and application value.

The utility model relates to the technical field of experimental equipment, and discloses a low-pollution phytopathology agar puncher which comprises a fixed cylinder, the inner wall of the fixed cylinder is connected with a sliding cylinder in a sliding mode, the upper surface of the sliding cylinder is fixedly connected with a sliding piece, the inner wall of the sliding cylinder is fixedly connected with a fixed column, and the fixed column is fixedly connected with the sliding piece. A connecting rod is rotatably connected to the outer wall of the fixing column, a clamping block is fixedly connected to the lower surface of the connecting rod, a first spring is fixedly connected to the outer wall of the clamping block, the outer wall of the clamping block is slidably connected to the interior of the sliding barrel, the outer wall of the clamping block is slidably connected to the inner wall of the fixing barrel, and a punching assembly is arranged on the lower surface of the fixing barrel. The punching assembly is used for punching. According to the utility model, the sliding barrel is driven by the sliding sheet, the fixed column is driven by the sliding barrel, the connecting rod is driven by the fixed column, and the clamping block is driven by the connecting rod, so that cleaning and sterilization treatment can be carried out more easily, the sterile state in the next use is ensured, and the accuracy of the experiment is further ensured.

This invention discloses a qPCR primer-probe combination, kit, and method for specifically detecting 16SrII group areca etiolated phytoplasma, belonging to the interdisciplinary field of molecular biology and plant pathology. The primer-probe combination includes a forward primer qTuf-F4-2 (SEQ ID NO:1), a reverse primer qTuf-R4 (SEQ ID NO:2), and a fluorescent probe qTuf-P4-1 (SEQ ID NO:3), targeting 16SrII group phytoplasma. tuf This gene conserved region demonstrates high specificity for 16SrII group areca yellowing phytoplasma in qPCR reactions, exhibiting no cross-reactivity with 16SrI group, 16SrXXXII group phytoplasma, areca genome, common pathogens, or endophytes. The limit of detection is 14.35 copies / μL, and the sensitivity meets the requirements for detecting low-load samples. The coefficient of variation for intra- and inter-group repeatability is less than 1%, indicating good stability. The kit and detection method developed based on this combination are simple to operate, highly sensitive, and provide stable results, making them suitable for the accurate diagnosis, pathogen typing, and field epidemic monitoring of areca yellowing disease, providing key technical support for the scientific control of 16SrII group phytoplasma.

The invention belongs to the technical field of molecular plant pathology, and particularly relates to a peanut bacterial wilt resistant gene AhPR10-54 and application thereof. According to the invention, through whole genome identification and bioinformatics analysis, 883 PR family genes (AhPR) are totally identified in peanuts, and the genes are distributed in 16 different subfamilies; further gene expression analysis shows that most AhPR genes can respond to induction of biological stress and abiotic stress; then 15 candidate genes are screened out for bacterial wilt inoculation treatment and plant hormone induction, and results show that AhPR10-54 and the like have significant up-regulation expression characteristics in disease-resistant peanut varieties. The AhPR10-54 gene is used for disease resistance identification, and the result shows that transient overexpression of AhPR-54 can induce hypersensitivity of plants and significantly improve the resistance of tobacco to ralstonia solanacearum. The invention provides an important basis for the cultivation of broad-spectrum disease-resistant varieties of plants.

The invention relates to the technical field of microbial genetic transformation and phytopathology, in particular to a fusarium solani fluorescence labeled strain and application thereof. The bacterial strain is classified and named as Fusarium solani (Fss9-YFP), and the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) NO: 42172. The fusarium solani fluorescence labeled strain is obtained by integrating a YFP fluorescent protein expression cassette sequence driven by a TEF-1alpha promoter into a protoplast of a wild type fusarium solani Fss1 strain. The method has the advantages that the fluorescently labeled strain Fusarium solani (Fss9-YFP) is stable in inheritance and good in fluorescence expression, and after the Fusarium solani (Fss9-YFP) is inoculated to soybean seedlings, germination and infection ways of spores are directly observed by adopting a microscope, so that distinguishing of disease-resistant soybean varieties, real-time tracing of soybean fusarium root rot, analysis of disease-resistant mechanisms and the like are facilitated.

This invention belongs to the fields of plant pathology and agricultural microbiology, specifically relating to a method for promoting efficient sporulation of *Anthracnose citrinum*, the obtained *Anthracnose citrinum* spore suspension, and its applications. The method involves inoculating *Anthracnose citrinum* mycelial cakes onto a high-concentration carrot agar medium, culturing them in a spore induction chamber under controlled temperature, humidity, and ventilation conditions until the mycelium covers the plate, then marking the medium with multiple parallel horizontal and vertical lines, adding glycerol aqueous solution to adjust the surface water activity to the range of 0.93–0.97, and applying a controlled spectral induction using alternating blue light and UV-A. Finally, a high-concentration spore suspension is obtained through elution. This invention significantly improves the sporulation yield and spore germination rate per plate while significantly reducing batch-to-batch variation. It is suitable for constructing standardized artificial infection models of *Anthracnose citrinum*, and can be used for pesticide efficacy evaluation, disease-resistant variety screening, and fruit storage and preservation research.

The invention relates to the technical field of phytopathology, in particular to an LAMP (loop-mediated isothermal amplification) composition for detecting ustilaginoidea virens toxin, a method and application. The detection method comprises the following steps: by taking DNA of rice seeds as a template, carrying out loop-mediated isothermal amplification on the DNA of the rice seeds by utilizing the first primer group, the second primer group or the third primer group to obtain an amplification result, and determining whether the rice seeds to be detected contain ustilaginoidea virens toxins or not. The method disclosed by the invention can be used for carrying out mass and rapid detection on toxins in a sample.

The invention belongs to the technical field of phytopathology and agricultural microorganisms, and particularly relates to a method for promoting efficient spore production of colletotrichum gloeosporioides, an obtained colletotrichum gloeosporioides spore suspension and application of the colletotrichum gloeosporioides spore suspension. The method comprises the following steps: inoculating a high-concentration carrot agar culture medium with a colletotrichum gloeosporioides cake, culturing in a spore induction cavity with controllable temperature, humidity and ventilation conditions until hyphae cover a flat plate, carrying out grid scribing according to a plurality of horizontal and vertical parallel lines, dropwise adding a glycerol aqueous solution to adjust the surface water activity of the culture medium to be 0.93-0.97, and culturing at the temperature of 20-30 DEG C to obtain the colletotrichum gloeosporioides. Controllable spectrum induction with blue light and UV-A alternating combination is applied, and finally high-concentration spore suspension is obtained through elution. According to the method, the single-plate sporulation quantity and the spore germination rate are remarkably improved, meanwhile, the batch-to-batch variation is remarkably reduced, and the method is suitable for constructing a standardized artificial infection model of the fruit anthracnose and is used for pesticide effect evaluation, disease-resistant variety screening and related research of fruit storage and preservation.

The application relates to the technical field of artificial intelligence, and discloses a method and device for identifying plant pathological types based on big data, which comprises the following steps: collecting a plant image of a preset plant, generating a labeled image of the plant image; performing image correction on the labeled image to obtain a corrected image of the labeled image; generating structural features of the preset plant according to the corrected image; performing image classification on the corrected image according to the structural features to obtain a classified image of the corrected image, generating a feature database of the classified image; acquiring a real-time image of a target plant, and generating a pathological type of the target plant according to the real-time image, a preset similarity algorithm and the feature database. The application can improve the identification efficiency of plant pathological types.

The application discloses an open environment-oriented single-stage crop leaf anomaly identification method, and belongs to the technical field of computer vision and plant pathology, and the method comprises the following steps: S1, data acquisition and preprocessing; S2, based on the data set of step S1, data enhancement is carried out; S3, a single-stage training model is constructed; S4, model training and reasoning are carried out.The application adopts the above-mentioned open environment-oriented single-stage crop leaf anomaly identification method, which not only reduces the difficulty of obtaining training data, simplifies the implementation process, improves the accuracy and efficiency of disease spot identification, but also integrates leaf segmentation and disease spot identification in the same deep learning framework, provides a practical and efficient tool for large-scale agricultural monitoring and plant pathology research, has good generalization ability and adaptability in processing leaf images of different crop types and various environmental conditions, and has wide application prospect and great practical value.