41 results about "Plant pathology" patented technology

The invention relates to the crossing field of agricultural disease monitoring and plant pathology, in particular to an alfalfa root rot prediction method based on pathogenic bacteria classification and data fusion. The method comprises the following steps: separating and purifying alfalfa root rot pathogenic bacteria and identifying types to construct a pathogenic bacteria database; carrying out an indoor artificial contamination test, and collecting hyperspectral data and binocular depth camera point cloud structure characteristics at different disease stages; in combination with multispectral / hyperspectral and point cloud data of a field unmanned aerial vehicle, multi-temporal information of healthy and diseased plants is tracked and marked. After vegetation index screening and principal component analysis dimensionality reduction, a multi-classification model is used for training, spectrum-structure feature generality of indoor and outdoor diseased plants is mined, and an early prediction model is constructed. According to the method, pathological diagnosis, spectral remote sensing and machine learning technologies are fused, cross-scale correlation analysis of pathogenic bacteria classification and field phenotypic characteristics is achieved, technical support is provided for accurate early warning of alfalfa root rot in the early stage, and the problems of traditional detection lagging and data isolation are solved.

The invention discloses a sesame fusarium wilt identification method based on a molecular marker, and relates to the technical field of phytopathology, the identification method comprises the following steps: S100, RNA extraction and purification: extracting total DNA and protein pollution RNA from a sesame sample to be detected, purifying the extracted RNA by using an efficient RNA extraction kit, removing existing impurities and inhibitors, and determining the content of the protein pollution RNA in the sesame sample to be detected; according to the method, specific long-chain non-coding RNA is adopted as a molecular marker, the molecular marker has high specificity and can accurately distinguish the sesame wilt from other diseases and healthy plants, the probability of misjudgment can be greatly reduced through data analysis, and the detection accuracy is improved. The specific long-chain non-coding RNA has the advantages that the specific long-chain non-coding RNA can be used for detecting the sesame fusarium wilt, reliable guarantee is provided for accurate identification of the sesame fusarium wilt, meanwhile, the specific long-chain non-coding RNA has good stability under different environmental conditions, and detection of a large number of samples can be completed within a short time through PCR amplification and high-throughput sequencing.

The invention relates to the technical field of molecular breeding and phytopathology, in particular to a KASP molecular marker of a wheat stripe rust resistant gene and application of the KASP molecular marker. The invention particularly relates to a functional SNP (Single Nucleotide Polymorphism) marker of a wheat stripe rust resistance major gene Chr6B excavated on the basis of Meta-GWAS (Complete Genome Association Analysis), and application of a KASP (Kinase Amplified Sequence Protein) molecular marker system developed on the basis of an SNP locus in wheat disease-resistant germplasm resource screening, molecular marker-assisted breeding and gene pyramiding breeding. The major effect of the MQTL6B.2 site on the Chr6B chromosome in resistance phenotypic variation is disclosed for the first time, and the contribution rate is greater than or equal to 28.54%. The primer can distinguish a disease-resistant material from a susceptible material, and provides an effective molecular marking tool for subsequent disease-resistant breeding. A molecular marker detection tool can be provided for identification of stripe rust-resistant germplasm resources and breeding of disease-resistant varieties, the selection efficiency is improved, and the breeding process is accelerated.

The present application relates to the technical field of plant pathology, and particularly relates to a method for artificially inducing large sporulation of Ascochyta aspidii, comprising the following steps: S1, strain activation: inoculating Ascochyta aspidii into PDA culture medium, and culturing under light at 20 DEG C for 7 days; S2, sporulation induction: inoculating the activated Ascochyta aspidii into an optimized sporulation culture medium, culturing under near-ultraviolet light at 20 DEG C for 14 days, and obtaining a large amount of spores, wherein the components of the optimized sporulation culture medium include 200 mL / L V8 juice, 0.3 g / L CaCO3, 1 g / L glucose, 1 g / L potassium nitrate, and 15-20 g / L agar. The present application establishes a rapid, efficient and simple in-vitro sporulation method of Ascochyta aspidii, which can effectively solve the technical problem of difficult sporulation under conventional culture conditions in a laboratory, and provides sufficient experimental materials for in-depth analysis of the pathogenic mechanism.

The application belongs to the technical field of molecular plant pathology, and particularly relates to a peanut disease-resistant gene AhNHL24 and application thereof. Through whole genome identification and bioinformatics analysis, 45 NHL family genes (AhNHL) are identified in peanuts, and gene expression analysis shows that most of the AhNHL genes can respond to the induction of biological stress and abiotic stress. Gene disease resistance identification finds that transient overexpression of AhNHL14, AhNHL24 and AhNHL31 can significantly improve the resistance of tobacco and peanuts to Pseudomonas solanacearum, and the disease resistance of AhNHL24 is the strongest. Through transgenic tobacco heterologous overexpression of AhNHL24 and inoculation experiments, it is shown that the peanut and tobacco plants overexpressing AhNHL24 not only have significantly enhanced resistance to Pseudomonas solanacearum, but also have significantly enhanced resistance to Sclerotium rofsii. The application provides an important basis for the cultivation of plant (peanut and tobacco, etc.) broad-spectrum disease-resistant varieties.

The invention discloses a common wild rice OrLRR1 gene and application thereof in improving rice blast resistance of rice, and belongs to the technical field of genetic engineering and phytopathology. The nucleotide sequence of the oryza vulgaris OrLRR1 gene is as shown in SEQ ID NO: 1. It is found that the OrLRR1 gene plays an important role in rice blast resistance of rice. The deletion of the OrLRR1 gene reduces the resistance of the rice to the magnaporthe oryzae, and the over-expression of the OrLRR1 gene can improve the resistance of the rice to the magnaporthe oryzae. The invention provides an application of an OrLRR1 gene or an encoding protein thereof in improving the resistance of rice to rice blast, and a method for cultivating rice blast-resistant rice by regulating and controlling the expression of OrLRR1. The invention provides a new gene resource and a theoretical basis for cultivating a new rice variety with high rice blast resistance, and has important application value.

The utility model relates to the technical field of plant culture, and discloses a novel plant pathology culture dish which comprises a first culture dish, a placing plate is arranged on the bottom wall of the first culture dish, connecting plates are fixedly connected to the left side and the right side of the placing plate, and a fixing plate is arranged on the bottom wall of the placing plate. Sliding grooves are formed in the left side and the right side of the fixing plate correspondingly, a rotating button is rotationally connected to the left side of the connecting plate, moving rods are fixedly connected to the upper end and the lower end of the outer wall of the rotating button correspondingly, a ball bearing is fixedly connected to the other end of each moving rod, and the ball bearings are fixedly connected to the left side of the outer wall of the connecting plate. According to the laboratory culture dish, the effect of fixing the placing plate is achieved, the fixing plate cannot deviate and collapse backwards, the fixing rods have the effect of fixing the telescopic rods, and the problem that the utilization rate of the space in the laboratory is low due to the fact that the culture dish occupies more vertical space in the limited space of the laboratory is solved.

The present invention relates to microbiology and plant pathology technology, and specifically to a method of using a small centrifuge tube to mix a small amount of drug sample liquid with water agar under the working condition of a microplate mixing station with a temperature system of 65°C to prepare an agar microplate containing drugs. The main components of the microplate mixing station include a temperature control body A, a centrifuge tube preheating component B for mixing samples, a drug sample preheating component C, a gun head preheating component D, and a glass slide preheating iron box component E. The main steps of the preparation method of the drug microplate are as follows: 1) preparing drug samples, 2) preparing water agar for mixing samples, 3) preparing gun heads, 4) starting the microplate mixing station to enter the working state, 5) preheating samples, 6) preheating glass slides, 7) transferring sample liquid, 8) transferring water agar, 9) mixing sample liquid and water agar, and 10) dripping and developing into a drug microplate. The advantages of the present invention are as follows: 1) small centrifuge tubes are commonly used consumables in biological laboratories and are convenient for use. 2) the total amount of drug samples required is very small; 3) the obtained drug microplate is convenient for subsequent biological assays and microscopic observations.

The utility model relates to the technical field of plant pathology research, and discloses a pressure-adjustable cassava leaf germ inoculator which comprises a box body and a gun body placed in the box body, the inner wall of the gun body is fixedly connected with a first clamping block and a second clamping block to fix an injector, and the inner wall of the gun body is fixedly connected with a second sliding rail and a second electric push rod. A first sliding rail is fixedly connected to the bottom end of the gun body, a supporting frame is slidably connected into the first sliding rail, the first electric push rod drives the supporting frame to move along the first sliding rail, a pressure sensor and a soft cushion are fixedly connected to the outer wall of the supporting frame, and the soft cushion corresponds to an injection opening of the injector. The second electric push rod is matched with the second sliding rail to achieve automatic quantitative inoculation, the pressure sensor and the soft cushion are matched with the first electric push rod to achieve pressure closed-loop adjustment, cassava leaves are effectively prevented from being damaged, and inoculation efficiency is remarkably improved.

The invention discloses a promoter Mig6p induced by pathogenic bacteria and application of the promoter Mig6p in rice disease resistance improvement, and belongs to the technical field of phytopathology and genetic engineering. According to the invention, a rice promoter Mig6p (the nucleotide sequence is as shown in SEQ ID NO.1) induced by various pathogenic bacteria is screened; the Mig6p background expression activity is very low, and the Mig6p is not induced by various abiotic stresses. According to the Mig6p: Lrd6-6E315Q rice expression vector, Mig6p starting lesion-like gene Lrd6-6 dominant negative regulation mutant Lrd6-6E315Q expression vectors are constructed, Mig6p: Lrd6-6E315Q rice is obtained through rice transformation, and verification shows that the broad-spectrum disease resistance of the Mig6p: Lrd6-6E315Q rice is remarkably improved, and the agronomic traits are not obviously influenced. The invention provides important guidance for improving the disease resistance of crops by using the scab-like gene, and also provides reference for improving other similar characters.

The invention relates to the technical field of biology, in particular to plant agrobacterium tumefaciens, application of the plant agrobacterium tumefaciens, a plant efficient infection preparation and an infection method. The strain of the plant agrobacterium tumefaciens is Agrobacterium tumefaciens MT-1, the strain is preserved in the Guangdong Microbial Culture Collection Center on October 22, 2025, and the preservation number is GDMCC No: 67146. The strain can efficiently infect plants. The agrobacterium tumefaciens MT-1 has a remarkable dip-dyeing effect on plants, particularly Chinese rose plants, can induce formation of cancer tumors in a short time and realizes efficient infection, so that the agrobacterium tumefaciens MT-1 has a good application value in efficient infection of the plants, and has a wide application prospect. A powerful experimental basis is provided for subsequent plant pathology research, related gene research and the like.

The application discloses a banana fusarium wilt secreted protein FoXYG1 and application thereof, and belongs to the technical field of molecular plant pathology. The secreted protein FoXYG1 can induce plant defense response, including causing plant cell hypersensitive necrosis, inducing callose accumulation and up-regulated expression of a plant immune-related pathway marker gene, activating SA, JA, ET and HR pathway-mediated defense pathways to induce plant immune response, and can significantly improve the resistance of plants to fusarium oxysporum and / or fusarium oxysporum. A new way is provided for improving plant disease resistance and reducing disease occurrence, and thus has a wide application prospect in the green development of modern agriculture.

The invention discloses a promoter Mig4p induced by pathogenic bacteria and application of the promoter Mig4p in rice disease resistance improvement, and belongs to the technical field of phytopathology and genetic engineering. According to the invention, a rice promoter Mig4p (the nucleotide sequence is as shown in SEQ ID NO.1) induced by various pathogenic bacteria is screened; the background expression activity of the Mig4p is very low. According to the Mg4p: Lrd6-6E315Q rice, the Mg4p: Lrd6-6E315Q rice is obtained by transforming the rice, the broad-spectrum disease resistance of the Mg4p: Lrd6-6E315Q rice is remarkably improved, and the agronomic characters of the Mg4p: Lrd6-6E315Q rice are not obviously influenced. The invention provides important guidance for improving the disease resistance of crops by using the scab-like gene, and also provides reference for improving other similar characters.

The invention discloses a promoter Mig1p induced by pathogenic bacteria and application of the promoter Mig1p in rice disease resistance improvement, and belongs to the technical field of phytopathology and genetic engineering. According to the invention, a rice promoter Mig1p (the nucleotide sequence is as shown in SEQ ID NO.1) induced by various pathogenic bacteria is screened; the background expression activity of the Mig1p is very low. According to the Mig1p: Lrd6-6E315Q rice expression vector, a Mig1p starting lesion-like gene Lrd6-6 dominant negative regulation mutant Lrd6-6E315Q expression vector is constructed, Mig1p: Lrd6-6E315Q rice is obtained through rice transformation, and verification shows that the broad-spectrum disease resistance of the Mig1p: Lrd6-6E315Q rice is remarkably improved, and the agronomic traits are not obviously influenced. The invention provides important guidance for improving the disease resistance of crops by using the scab-like gene, and also provides reference for improving other similar characters.

The application belongs to the cross field of agricultural information technology and plant pathology, and provides a detection method for resistance of Chinese rose to powdery mildew based on beta-alanine, which comprises the following steps: step 1, measuring the content of beta-alanine in Chinese rose leaves; step 2, establishing an analysis model between beta-alanine and Chinese rose powdery mildew; and step 3, establishing a resistance grading model to output different levels of resistance; the application can accurately quantify the resistance of Chinese rose to powdery mildew by measuring the content of beta-alanine in Chinese rose leaves and establishing an analysis model between beta-alanine and Chinese rose powdery mildew.

The invention discloses red fluorescent ralstonia solanacearum as well as a preparation method and application thereof, and relates to the technical field of pathogenic bacterium detection in molecular biology and plant pathology. Red fluorescence labeled strains can be directly and clearly observed in different tissues after the eucalyptus is infected by the Ralstonia solanacearum, the method can be used for exploring the process of infecting the eucalyptus by the Ralstonia solanacearum, the visualization of the process of infecting the eucalyptus by the Ralstonia solanacearum in the different tissues is realized, the process of infecting the eucalyptus by the Ralstonia solanacearum in the root system of the eucalyptus can be quickly observed, and the method has a good application prospect. The bacterial wilt resistance of different eucalyptus strains is evaluated, a powerful tool is provided for studying the pathogenic mechanism of the ralstonia solanacearum and screening disease-resistant strains, and a visual and reliable technical basis is also provided for formulating a prevention and control strategy of the bacterial wilt of the eucalyptus. The method can be used for observing the process of infecting eucalyptus tissues by ralstonia solanacearum in real time at an early stage, and does not need to observe and evaluate after diseases occur, so that the time is shortened. The fluorescent strain has very important significance on identification of main pathogenic bacteria of eucalyptus bacterial wilt and tracking of infected parts.

The invention belongs to the technical field of molecular plant pathology, and particularly relates to a peanut disease-resistant gene AhNHL24 and application thereof. Through whole genome identification and bioinformatics analysis, 45 NHL family genes (AhNHL) are totally identified in peanuts, and gene expression analysis shows that most AhNHL genes can respond to induction of biological stress and abiotic stress. The gene disease resistance identification finds that the instantaneous overexpression of AhNHL14, AhNHL24 and AhNHL31 can significantly improve the resistance of tobacco and peanut to ralstonia solanacearum, and the disease resistance of AhNHL24 is the strongest. The transgenic tobacco heterologous overexpression AhNHL24 and inoculation experiments show that not only is the resistance of peanut and tobacco plants for overexpression AhNHL24 obviously enhanced to ralstonia solanacearum, but also the resistance to sclerotium rolfsii is also obviously enhanced. The invention provides an important basis for cultivation of broad-spectrum disease-resistant varieties of plants (peanuts, tobaccos and the like).

The present invention relates to the technical field of plant pathology, and specifically to a method and device for identifying the sensitivity of citrus canker varieties, comprising a box body and a top plate installed on the box body: an injection assembly for injecting leaves in the method for identifying the sensitivity of citrus canker varieties, the injection assembly is arranged inside the box body, and the injection assembly is used to inject pathogens into citrus leaves; a conveying assembly, the conveying assembly is arranged above the injection assembly, and the conveying assembly is used to convey citrus leaves into the injection position of the injection assembly; a mounting frame, the mounting frame is arranged at one end of the injection assembly, a conveyor belt is arranged above the mounting frame, and a driving motor is arranged on one side of the conveyor belt. Compared with the prior art, the present application realizes smooth transportation after injection by providing a conveying assembly to cooperate with the injection assembly, and ensures the stability and efficiency of the injection operation through the automatic control and precise positioning of the conveying assembly, avoiding errors that may be caused by manual intervention.

The invention relates to the technical field of phytopathology and agricultural biology, and particularly provides an inducer for inducing cucumber to resist corynespora leaf spot, an induction method and application. The inducer comprises caffeic acid, and the optimal application scheme is that the concentration of caffeic acid is 100 mu mol / L, the spraying interval is 3 days, and the caffeic acid is sprayed for three times in total. The inducer and the induction method provided by the invention are used for inducing cucumber to resist the corynespora leaf spot disease, have the characteristics of environmental protection and high efficiency, are beneficial to reducing the use of chemical pesticides, and ensure the safety, yield and quality of cucumber production.

The utility model relates to the technical field of plant pathology, and discloses a plant pathogen pathogenic gene target screening device which comprises a screening assembly, and a first fixing assembly used for preventing the screening assembly from shaking is arranged on the upper side of the screening assembly. According to the plant pathogen pathogenic gene target screening device, the conical flask is clamped and fixed through the first fixing assembly and the second fixing assembly, so that the relative position between the conical flask and the fixing table is fixed, the phenomenon that a culture medium is leaked due to displacement, toppling or violent shaking of the conical flask when a shaking table oscillates is avoided, and meanwhile, the screening efficiency is improved. Beneficial bacteria and pathogenic bacteria are completely separated in space, thallus mixing is fundamentally avoided, the premise is created for single strain research, active substances secreted by the beneficial bacteria in the conical flask are continuously and directionally conveyed to the pathogenic bacteria environment on the basis of physical isolation, dynamic intervention is achieved, and the screening efficiency is improved. And a stable and controllable special model is provided for experimental operation.

The invention discloses an anti-streptomycin gene as well as an encoding protein and application thereof, and belongs to the technical field of phytopathology. The invention discloses a novel streptomycin resistance gene aphh (3) derived from tomato canker pathogen. The gene is located on a newly found plasmid pCM3 of tomato canker pathogen, and is used for coding a protein APH (3) belonging to an aminoglycoside antibiotic phosphotransferase family. The functions of the gene are verified through tests of gene knockout, function complementation, heterologous expression and the like, meanwhile, mass spectrometric analysis proves that APH (3) can catalyze streptomycin molecules to generate phosphorylation reaction to generate streptomycin monophosphate, so that the inhibitory activity on bacteria is lost, and a strain carrying the gene has remarkable resistance to streptomycin. The streptomycin resistance gene aphh (3) can be widely applied to the fields of pathogenic bacterium resistance mechanism research, resistance strain screening, molecular marker selection and the like, and has important scientific research and application values.

The invention discloses a magnaporthe oryzae inoculation device and method, and relates to the technical field of plant pathology research instruments.The magnaporthe oryzae inoculation device comprises an inoculation box, the top of the inoculation box is fixedly connected with a water tank used for keeping the humidity of the humid environment in the inoculation box, and two inoculation assemblies used for inoculation are arranged outside the inoculation box; the bottom of the inoculation box is fixedly connected with a control box; and an observation window for observing the growth condition of rice after internal inoculation is arranged on the outer wall of the inoculation box. Through the opposite-direction rotation system of the flow guide plate and the culture tank, the inclined plane design of the flow guide plate and the inclined plane design of the circular baffle and the flow guide plate, uniform coverage of rice leaves by liquid medicine, improvement of inoculation efficiency and liquid medicine utilization rate, integration of light supplement, humidity control and waste liquid separation functions, optimization of experimental conditions and reduction of operation complexity are realized; the growth condition of the rice can be recorded without opening the device through the observation window and the transparent circular baffle, and environmental interference is reduced.

The present application relates to the technical field of plant pathology and agricultural science research, more particularly to a rice seedling blight inducing device and method, wherein the device comprises a box body, a box cover, a first measuring cup and a second measuring cup, the box cover is detachably connected to the top of the box body, the box body is a transparent structure, the box cover is provided with air holes, the bottom of the box body is provided with a drainage hole, the side wall of the box body is provided with a scale, the capacity of the first measuring cup is greater than that of the second measuring cup, and the first measuring cup can be inverted to cover the second measuring cup to form a closed germination cavity. The present application has the advantages of simple structure and facilitating operation standardization; through the sandwich type matrix structure of "bottom moisture layer + seed layer + top covering layer", combined with the two-stage culture mode of indoor constant temperature disease induction and outdoor natural adversity induction, the disease incidence is significantly improved, and disease identification is facilitated.

This invention discloses a pathogen-induced promoter Mig6p and its application in improving disease resistance in rice, belonging to the fields of plant pathology and genetic engineering. This invention screened a rice promoter Mig6p (nucleotide sequence shown in SEQ ID NO.1) induced by multiple pathogens; Mig6p exhibits very low basal expression activity and is not induced by various abiotic stresses. Furthermore, a dominant-negative regulatory mutant of the Mig6p promoter-induced lesion gene Lrd6-6, Lrd6-6, was constructed. E315Q The expression vector was obtained by transforming rice to obtain Mig6p:Lrd6-6 E315Q Rice, verified, Mig6p:Lrd6-6 E315Q The broad-spectrum disease resistance of rice was significantly improved, while agronomic traits were not significantly affected. This invention provides important guidance for improving crop disease resistance using lesion-like genes and also provides a reference for improving other similar traits.

The invention relates to microbiology and plant pathology technology, and specifically to a method of using a plastic culture dish to mix a small amount of drug sample liquid with water agar under the working condition of a micro-plate mixing station with a temperature system of 65°C to prepare an agar micro-plate containing drugs. The main components of the micro-plate mixing station include a temperature control body A, a plastic culture dish preheating component B for mixing samples, a drug sample preheating component C, a gun head preheating component D, and a glass slide preheating glass plate component E. The main steps of the preparation method of the drug micro-plate are as follows: 1) drug sample preparation, 2) sample mixing and water agar preparation, 3) gun head configuration, 4) starting the micro-plate mixing station to enter the working state, 5) sample preheating, 6) glass slide preheating, 7) absorbing sample liquid, 8) absorbing water agar, 9) mixing sample liquid with water agar, and 10) dripping to develop into a drug micro-plate. The advantages of the invention are as follows: 1) plastic culture dishes are conventional consumables in the field of biology and are easy to obtain; 2) the total amount of drug samples required is very small; 3) the prepared drug micro-plate is convenient for subsequent biological assays and microscope observations.

The invention relates to the technical field of agricultural microbiology and phytopathology, in particular to application of rhodopseudomonas palustris in prevention and treatment of rice blast, by spraying a bacterial suspension of the strain with the concentration of 1 * 10 < 8 > CFU / mL to leaf surfaces of rice in the three-leaf one-core stage, a rice linolenic acid metabolic pathway can be regulated and controlled, and the rice blast resistance is improved. The accumulation of an endogenous disease-resistant metabolite 12-oxygen-plant dienoic acid is obviously induced. The OPDA inhibits the magnaporthe oryzae through a dual mechanism, the area of a plate inhibition zone reaches 94.5% + / -2% when the concentration is 50 micrograms / mL, the spore germination inhibition rate reaches 97.3% + / -1.2% (repeating for three times), and a rice jasmonic acid defense system can be activated. The disease-resistant molecular mechanism is defined, the control effect is equivalent to that of chemical pesticides, and a new strategy is provided for developing efficient and stable microbial-source rice blast-resistant preparations.

The invention discloses a simple and rapid rubber tree powdery mildew vacuole observation method, and belongs to the technical field of phytopathology and microbiological microscopic observation. The invention discloses a simple and rapid method for observing vacuoles of powdery mildew of a rubber tree. The vacuoles of powdery mildew are observed by using cytochemical staining and a microscopic technology. The form of the vacuoles treated by the method is not influenced, the form and the number of the vacuoles are easy to microscopically observe, and a foundation is laid for developing invasion of the powdery mildew of the rubber trees, responding to a drought stress mechanism and the functions of the vacuoles; technical support is provided for studying the growth and development state and metabolic activity of the bacterium, regulating the transport process of cell environment substances and the like.

The invention relates to the technical field of plant pathology detection, in particular to a potato late blight molecular identification method of a molecular marker primer, and the primer comprises a molecular marker primer pair for identification of Phytophthora infestans (Phytophthora infestans). The primer pair comprises specific sequences of three continuous adenine bases (AAA) in a targeted pathogenic bacterium ribosome internal transcriptional spacer (ITS), and comprises a forward primer, a reverse primer, a reverse primer and a reverse primer, the nucleotide sequence of the forward primer is shown as SEQ ID NO: 1, and the nucleotide sequence of the reverse primer is 5 '-GCTGCGTCTTCTATGATGC-3'; the nucleotide sequence of the reverse primer is as shown in SEQ ID NO: 2: 5 '-TCCTCCGCTTATTGATATGC-3'. The nucleotide sequence of the reverse primer is as shown in SEQ ID NO: 2. According to the potato late blight molecular identification method of the molecular marker primer, 100% specificity of the primer pair is achieved through base mismatch design, detection of samples such as tubers and soil can be completed within 4 hours by combining optimized CTAB / magnetic bead method extraction and 56 DEG C annealing PCR technology, the sensitivity reaches 10 pg / mu L, and the primer pair is high in specificity and high in specificity. The method is suitable for early warning and accurate prevention and control of potato late blight.

This invention discloses a streptomycin resistance gene, its encoded protein, and its applications, belonging to the field of plant pathology technology. This invention discovers a novel streptomycin resistance gene derived from *Tomato Canker*. aph(3) This gene is located on a newly discovered plasmid pCM3 of *Tomato Canker*, encoding a protein APH(3) belonging to the aminoglycoside antibiotic phosphotransferase family. This invention verified the function of this gene through gene knockout, functional complementation, and heterologous expression experiments. Simultaneously, mass spectrometry analysis demonstrated that APH(3) catalyzes the phosphorylation of streptomycin molecules, generating streptomycin monophosphate, thereby eliminating its inhibitory activity against bacteria. Strains carrying this gene exhibit significant resistance to streptomycin. This invention relates to a streptomycin resistance gene. aph(3) It can be widely used in the study of pathogen resistance mechanisms, screening of resistant strains, and selection of molecular markers, and has important scientific research and application value.

The application belongs to the field of plant pathology and fungal disease control, and particularly relates to siRNA for inhibiting Magnaporthe oryzae and a use method and application thereof, a sequence of a sense strand of the siRNA is shown as SEQ ID NO. 8, a sequence of an antisense strand of the siRNA is shown as SEQ ID NO. 9, the siRNA can be used for preparing nucleic acid pesticides for inhibiting Magnaporthe oryzae or preventing and treating rice blast, a concentration of the siRNA in the nucleic acid pesticide is greater than or equal to 20 ng / μL, and preferably the concentration is 20 ng / μL. The nucleic acid pesticide prepared by using the siRNA has simple use method and small dosage, the nucleic acid pesticide can be applied on a Magnaporthe oryzae strain, and has a significant inhibiting effect on pathogenicity of the Magnaporthe oryzae, expression of lncRNA5, turgor pressure accumulation in an appressorium and formation of penetration pegs; the nucleic acid pesticide can also be applied on a plant, and can be used for preventing and treating rice blast, and the siRNA has a wide application prospect in the field of fungal disease control.