48 results about "Plant pathology" patented technology

The invention belongs to the technical field of biology and in particular provides a pectate lyase (PL) gene Pcpel1 which is cloned from phytophthora capsici and protein preparation technology thereof. Gene and protein levels prove that the gene is effectively involved in the process that the phytophthora capsici infects hot pepper hosts and results in occurrence of the course of diseases on hot pepper leaves. Plant pathology and cytochemistry technology further prove that after the protein coded by the gene is inoculated onto the hot pepper leaves, obvious withering and shrinking occur on theinoculated parts of the leaves and the cell walls on the affected parts of the leaves are obviously degraded, namely the gene code is an important protein related to the course of diseases or is possibly an important target pathogenic gene of a phytophthora capsici PL gene cluster. The invention provides important technical reserve for further developing phytophthora capsici molecule detection technology.

The invention relates to the plant pathology field, and specially relates to a quantitative dip in bacterial suspension method for identifying resistance to cotton verticillium wilt using vermiculite sandy soil bottomless bowl of paper. The method in the invention includes the following steps: 1) making a nutrient bowl by mixing vermiculite and sandy soil and putting them into a paper bowl; 2) sowing and culturing cotton sprouts; 3) inoculating pathogen, which requires inoculating bacterium spore suspension of cotton verticillium wilt when one true leaf of a cotton sprout grows to be flat, wherein the inoculation concentration is in the range of from 1*10<6> to 5*10<6> spores per ml, and every cotton sprout is inoculated with bacteria liquid about from 2 to 3 ml; 4) evaluating the resistance to cotton verticillium wilt. The invention is characterized by being fast, accurate, economical, environmental beneficial and so on.

The invention belongs to the technical field of biology and in particular provides a polygalacturonase (PG) gene Pcipg5 which is cloned from phytophthora capsici and protein preparation technology thereof. Gene and protein levels prove that the gene is effectively involved in the process that the phytophthora capsici infects hot pepper hosts and results in occurrence of the course of diseases on hot pepper leaves. Plant pathology and cytochemistry technology further prove that after the protein coded by the gene is inoculated onto the hot pepper leaves, obvious withering and shrinking occur on the inoculated parts of the leaves and the cell walls on the affected parts of the leaves are obviously degraded, namely the gene code is an important protein related to the course of diseases or is possibly an important target pathogenic gene of a phytophthora capsici PG gene cluster. The invention provides important technical reserve for further developing phytophthora capsici molecule detection technology.

The invention discloses a rubber tree powdery mildew in vitro culture method and a culture medium thereof, belonging to the technical field of plant pathology. The rubber tree powdery mildew in vitro culture method comprises the following steps of: firstly, preparing a nutrient solution and a culture disc; secondly, preparing an in vitro leaf; and thirdly, inserting the in vitro leaf after treating into a PCR plate hole filled with the nutrient solution, lightly brushing powdery mildew on an infected rubber tree leaf on the in vitro leaf which is treated with the nutrient solution by using a writing brush or a brush which is sterilized in 75 percent alcohol, arranging in an artificial climate box with the temperature of 23 DEG C and the moisture of 90 percent, carrying out light culture for 16h per day and carrying out dark culture for 8h per day. The rubber tree powdery mildew in vitro culture method has the advantages of convenience, durability and high efficiency and can be used for culturing rubber tree powdery mildew in a large scale, thereby realizing in vitro culture of the rubber tree powdery mildew on the leaf and solving the problems of culture and preservation of the rubber tree powdery mildew.

An apparatus and methods for non-destructively analyzing the strength of plant roots and stalks, and uses of this information in plant breeding, crop production, and detection of plant pathology. The apparatus and methods involve the use of a variable rate tensioner that may be automatically or remotely adjusted based on crop conditions.

The invention discloses a plant active ingredient of Cynanchum Komarovii extract with 7-defluvium unguium oxyl tylophorine as effective component and accessory ingredient of plant source antiviral agent preferably water, emulsifiable concentrate and wettable powder; preparation method and application against plant virus.Through indoor farm testing, the antiviral preparation can be used for preventing and treating pathology caused by single virus infecting different host or two or more viruses mixed infecting the same host or homogeneous host, especially plant pathology caused by 5 virus distributing world widely with severe hazard. The preventing and treating effect can reach up to 40-50%. The antiviral preparation has ideal effect in preventing and treating multi-viruses mixed infected tobacco. The inventive antiviral agent has no toxicity to human and livestock and high safety and performance.

The invention relates to a method producing trichodermin prophylactic by utilizing remained activated sludge and agriculture and forestry scrap biomass, which belongs to the technical field of biopesticide and environmental pollution treatment. The method mainly takes the remained activated sludge and scrap biomas generated during sewage disposal as the producing raw material and producing the trichodermin prophylactic by adopting the method of solid state fermentation. The manufacturing technique comprises: (1), crushing and pretreating scrap biomass; (2), preparing culture medium of sludge and biomass; (3), inoculating trichoderma viride after sterilizing the culture medium for solid state fermentation; (5), appending stabilizer and auxiliary agent into the leavening after being dried. The method can be used for crushing and packing and also can be directly used in agricultural soils, which can prevent and cure soil-borne disease and improve soil and increase soil fertility. The trichodermin prophylactic produced with the technics can effectively prevent and cure various diseases caused by plant pathology with low producing cost; besides, the product can effectively treat the castoff, thus reducing the pollution for environment.

The invention relates to the field of plant pathology, in particular to cotton endophytic bacteria YUPP-10 and application thereof in cotton verticillium wilt controlling. The strain preservation number of the cotton endophytic bacteria YUPP-10 is CCTCC No:M2017141. The experimental result shows that the cotton endophytic bacteria YUPP-10 has inhibitory effect on the growth of verticillium wilt pathogen, corn vermiculite culture of the cotton endophytic bacteria YUPP-10 has a high controlling effect on the verticillium wilt, and the cotton endophytic bacteria YUPP-10 is suitable as a biological resource for verticillium wilt controlling. The cotton endophytic bacteria YUPP-10 and application in cotton verticillium wilt controlling provide new resources for enriching biological controllingof agricultural diseases and has practical application guidance for pollution-free green agricultural systems.

The invention provides cytospora chrysosperma protoplast preparation and genetic transformation methods, and belongs to the field of molecular plant pathology. The cytospora chrysosperma protoplast preparation method comprises the following steps: inoculating cytospora chrysosperma into a YEPD liquid culture medium for mycelium culture, and performing enzymolysis treatment on the cytospora chrysosperma mycelia, thereby obtaining protoplast. In addition, a PEG mediated method is adopted for genetic transformation on the prepared protoplast, and thus a mutant can be obtained. The method provided by the invention is high in protoplast preparation efficiency, low in cost and simple to operate, the number of protoplast after enzymolysis can be as high as 10<8>-10<9>/mL, the transformation efficiency is high, the protoplast is good in activity, the transformation efficiency can be improved, and the research time can be greatly shortened. By adopting the method, a good base is made for molecular biology study on cytospora chrysosperma in the future, and furthermore potential utilization values can be made for prevention and treatment and histopathologic study on cytospora chrysosperma.

The invention provides a method for rapid separation of phytophthora capsici and a special culture medium thereof, and belongs to the technical field of phytopathology research. The separation method comprises the steps of sick sample treatment, disinfection, culture medium preparation, and the like. The sick sample is selected from a freshly-picked sick stem, and a sick sample tissue of 9-11 cm is taken with a juncture of the sick part and the healthy part of the sick sample as a center; the tissue is cleaned with tap water, and is dried in the air to remove surface moisture; the dried tissue is uniformly sprayed with 75% alcohol, then soaked with a 1% sodium hypochlorite solution, and cleaned with sterile water; after drying in the air, the disinfected large block of the sick sample is cut into small blocks on an aseptic operating floor; and separation culture is performed with special culture medium. With the method of the invention, the separation rate of phytophthora capsici reaches 81.99% on average, which is increased by 32.6% when compared with the separation rate of a routine (small block disinfection treatment, small block separation) treatment method on average; the separation time is only 31.6 minutes on average; the separation time is shortened by 50.1% on average; and the operations are simple and practical.

The invention provides peanut ralstonia solanacearum effect protein RipAU, and belongs to the field of research on plant pathology and crop disease control. The peanut ralstonia solanacearum RS-P.362200 effect protein RipAU has an amino acid sequence as shown in SEQ ID No.2, and the nucleotide sequence of the coding gene is as shown in SEQ ID No.1. The invention also discloses a preparation method of the Ralstonia solanacearum RS-P.362200 effect protein The RipAU gene is transiently expressed in nicotiana benthamiana, and an allergic necrosis reaction can be generated. After the RipAU is knocked out, the pathogenicity of the ralstonia solanacearum to the peanuts can be remarkably reduced, and it is indicated that the RipAU serves as a virulence factor. According to a yeast two-hybrid method, a target protein AhSBT1.7 of RipAU is screened out. The invention has important application value in the aspects of disclosing the pathogenesis of ralstonia solanacearum and preventing and treating peanut bacterial wilt.

The invention relates to a disease resistance function of a gene of a model plant arabidopsis thaliana, and belongs to the field of plant pathology research. The invention discloses an arabidopsis thaliana gene AT5G02580. The nucleotide sequence of the arabidopsis thaliana gene AT5G02580 is as shown in SEQ ID NO: 1. The invention further discloses application/functions of the arabidopsis thaliana gene AT5G02580. The disease resistance of plants can be remarkably improved by knocking out the AT5G02580 gene in arabidopsis thaliana. According to the invention, an sgRNA sequence of AT5G02580 gene targeted knockout is firstly designed, then a carrier is constructed, and arabidopsis thaliana is subjected to genetic transformation by the carrier, so that a corresponding transgenic plant is obtained, and two strains ko-1 and ko-2 of different mutation types of the AT5G02580 gene are identified from the transgenic plant.

The invention relates to the application of natural pyrethrum emulsifiable concentrate preparation as bactericide for killing wheat scab pathogen and wheat Rhizoctonia cerealis. The invention is pure natural plant source bactericide, its solvent, synergist, and stabilizer are all plant extracts. It has no residues as pyrethrin will decompose into water and carbon dioxide in nature. The preparation has low toxicity and no residues, generates no pesticide toxicity to the seedling, flower, and fruit of plants, even overcommiting. The inventive pyrethrum emulsifiable concentrate preparation can be used for preventing and treating plant pathology, and exploits new field for the application of pyrethrum. The inventive pyrethrin emulsifiable concentrate preparation can be used as bactericide in the production of green food and organic food due to its no residue.

The invention relates to phytopathology, in particular to a setosphaeria turcica attenuated strain STAM-226 and application thereof, and particularly relates to the setosphaeria turcica attenuated strain and application thereof in prevention and treatment of northern leaf blight of corn. Through ATMT conversion, screening, identification, pot culture experiments and field experiments, a setosphaeria turcica attenuated strain with a significant control effect on the northern leaf blight of corn is obtained, and the preservation number is CGMCC NO.20269. Test results show that the setosphaeria turcica attenuated strain is firstly inoculated, the setosphaeria turcica virulent strain STAM-YC is inoculated after 1-3 days, and a remarkable mild strain cross protection (MSCP) effect is shown in each growth period. The STAM-YC is inoculated after the setosphaeria turcica attenuated strain is inoculated for 2 d, so that the prevention and control effect on the northern leaf blight of corn is most remarkable, reaching 89.6%. The setosphaeria turcica attenuated strain for preventing and treating the northern leaf blight of corn is safe to people, livestock, insects and crops, and is environmentally friendly.

The invention relates to the fields of microorganisms and phytopathology, and particularly discloses a method for counting plant pathogenic oomycetes zoospores. According to the invention, the cameraphase parameter is set, so that the two morphological characteristics of the zoospores, namely blue spores and orange spores, can be clearly observed, thereby realizing the counting of the zoospores.According to the method, the zoospores and the resting spores can be counted simultaneously, so that the related experiments of the oomycetes zoospores are facilitated, and the accuracy and the precision of experimental results are improved. The method disclosed by the invention is simple and reliable, and all microscopes with imaging equipment can be suitable for counting oomycetes zoospores, sothat an evaluation index is provided for evaluating the severity of plant diseases and the pesticide effect of the bactericide.

A system and method for identifying a probability of disease affecting a crop based on data received over a network is described herein, and may be implemented using computers for providing improvements in plant pathology, plant pest control, agriculture, or agricultural management. In an embodiment, a server computer receives environmental risk data, crop data, and crop management data relating to one or more crops on a field. Agricultural intelligence computer system 130 computes one or more crop risk factors based, at least in part, the crop data, one or more environmental risk factors based, at least in part, the environmental data, and one or more crop management risk factors based, at least in part, on the crop management data. Using a digital model of disease probability, agricultural intelligence computer system 130 computes a probability of onset of a particular disease for the one or more crops on the field based, at least in part, on the one or more crop risk factors, the one or more environmental risk factors, and the one or more crop management factors.

The invention belongs to the technical field of wheat aphid physiology and phytopathology, and particularly relates to a piercing-sucking type insect thin film virus feeding method and application thereof. The method comprises the following steps: (1) uniformly thinning a plastic film, and covering the upper end of a container with openings at the two ends with the plastic film; (2) dropwise adding a virus feeding solution on the thin film; (3) uniformly thinning the plastic film, and covering the virus feeding solution with the plastic film; (4) inclining the opening at the lower end of the container upwards, and putting the insects into the container through the opening at the lower end; and (5) putting the container filled with the insects onto absorbent paper with the lower end opening facing downwards, and putting the container onto a plane for virus feeding. The method is simple and convenient to operate, effectively improves the efficiency of sucking food or virus solution by the insects, and provides convenience for further research on the virus transmission efficiency between the insects and plants.