48 results about "Plant pathology" patented technology

The invention belongs to the field of molecular plant pathology, and particularly relates to a preparation method of a green fluorescent protein marked coconut stem bleeding disease bacterium protoplast. The preparation method has the beneficial effects that appropriate conditions for protoplast formation are determined, the high-quality protoplast is prepared by using 1mol / L mannitol as an osmotic stabilizer and 7.5mg / mL driselase as lyase, GFP (green fluorescent protein) is transferred to a coconut stem bleeding disease bacterium so as to obtain a stable genetic GFP pathogenic bacterium strain; therefore, a solid foundation is laid for genetic operations of the disease bacterium, such as GFP transformation and genetic expression, the cost is saved, the working efficiency can be greatly improved, and the research time is shortened; and meanwhile, the green fluorescent protein marked coconut stem bleeding disease bacterium facilitate the research on histopathology, disease epidemiology and the like of the disease.

The invention relates to phytopathology, in particular to cotton fungal endophyte CEF-082 and an application of the cotton fungal endophyte CEF-082 in prevention and treatment of cotton verticillium wilt. The preservation serial number of the cotton fungal endophyte CEF-082 is CGMCCNo.11313; the suppression rate of the metabolite of the CEF-082 to colony growth of the cotton verticillium wilt reaches 100%, and the suppression rate to the conidium yield is 70.73%. Greenhouse experimental results show that cotton is pre-inoculated through CEF-082 culture filtrate when true leaves of cotton seedlings appear for the first time, and the control effect on the cotton verticillium wilt is 62.37%; the corn sand grain culture of the CEF-082 is connected into a matrix at the percentage of 2%, and then the cotton seedlings are cultivated, so that the control effect on the cotton verticillium wilt is 66.88%. Compared with bacillus subtilis which is greatly used in production, the control effect is increased by 30.7% on average; the expressions of defense related genes beta-1,3-glucanase, POD, PPO, PAL and the like are improved by inoculating the cotton through the CEF-082, hence, the resistance of plants to the cotton verticillium wilt is enhanced, and the damage of pathogenic bacteria is reduced.

The invention relates to a method producing trichodermin prophylactic by utilizing remained activated sludge and agriculture and forestry scrap biomass, which belongs to the technical field of biopesticide and environmental pollution treatment. The method mainly takes the remained activated sludge and scrap biomas generated during sewage disposal as the producing raw material and producing the trichodermin prophylactic by adopting the method of solid state fermentation. The manufacturing technique comprises: (1), crushing and pretreating scrap biomass; (2), preparing culture medium of sludge and biomass; (3), inoculating trichoderma viride after sterilizing the culture medium for solid state fermentation; (5), appending stabilizer and auxiliary agent into the leavening after being dried. The method can be used for crushing and packing and also can be directly used in agricultural soils, which can prevent and cure soil-borne disease and improve soil and increase soil fertility. The trichodermin prophylactic produced with the technics can effectively prevent and cure various diseases caused by plant pathology with low producing cost; besides, the product can effectively treat the castoff, thus reducing the pollution for environment.

The invention relates to plant pathology technology and photography technology, and specifically discloses an auxiliary device for aseptic photography and an application method thereof. The auxiliary device is mainly composed of a stable frame, a camera frame and a dish frame. The auxiliary device needs to be used in conjunction with the ultra-clean workbench. The main steps of the application method are as follows: 1) Running the ultra-clean workbench: 2) Assembling the auxiliary device and the camera: 3) Mounting and adjustment of the materials to be photographed: 4) Taking pictures : 5) Operate after taking pictures. The advantages of the present invention are: 1) taking pictures under sterile conditions, which can effectively reduce the risk of biological contamination caused by taking pictures, and is convenient for continuous tracking and photographing records of the growth of pathogenic bacteria; 2) it can eliminate the shaking of directly holding the camera, and the image is clear. It is easy to obtain high-definition pictures; 3) It can provide accurate scale and size for post-processing of photos.