The invention relates to the technical field of pet excrement treatment, particularly to an odor removing agent special for pet excrement treatment, a preparation method and application thereof, and aims to solve the problems that cat litter has poor deodorization effect during use and cannot effectively remove harmful microorganisms in excrement. According to the invention, a complex microbial inoculant, montmorillonite powder, activated carbon powder, citric acid, fermented lentinus edodes powder, bran powder and corn flour are reasonably collocated to prepare the odor removing agent, and the odor removing agent is added as an addition material of cat litter into cat litter for use. Deodorization performance tests find that the odor removing agent can greatly lower the concentration of ammonia gas, and the deodorization and odor removing effects are significant, and bacteriostatic and bactericidal tests find that the odor removing agent can effectively inhibit the growth and propagation of Escherichia coli, salmonella and other harmful microorganisms in pet excrement, and has good bacteriostatic and bactericidal effects, therefore the odor removing agent can effectively improve the use effect of cat litter, and has significant deodorizing, sterilizing and bacteriostatic effects.