Method for preserving primary colors of red and yellow plant disease specimens
A plant disease and preservation method technology, applied in the field of phytopathology, can solve the problems of easy-splitting fruit, little research on preservation technology, poor color retention effect, etc., and achieves the effects of safe and environmentally friendly raw materials and simple and easy production methods.
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Embodiment 1
[0019] The red and yellow two-color plant disease specimen primary color preservation method of the present invention is used for the preservation of yellow loquat anthracnose, and the specific steps are:
[0020] 1) Loquat anthracnose specimens were collected, arranged and sterilized. Collect clusters of 7-8 mature loquat anthracnose specimens with single and typical symptoms, remove redundant old leaves and other diseased fruits, and leave diseased fruits with typical symptoms and healthy and normal fruits. Complete specimens with fruit branches, fruit stalks, fruit pedicles, diseased fruits, and healthy fruits. After pruning, clean the complete specimen with clean water, soak it in 70% alcohol for 3-4 minutes, and wash it with distilled water 3-4 times.
[0021] 2) The color is fixed. Put the sterilized and cleaned loquat anthracnose specimens into the fixative solution. The specimen occupies no more than 80% of the space of the fixative solution, and the specimen should ...
Embodiment 2
[0028] The red and yellow two-color plant disease specimen primary color preservation method of the present invention is used for the preservation of red tomato late blight, and the specific steps are:
[0029] 1) Collection and sterilization of tomato late blight specimens. Collect clusters of 7-8 mature tomato late blight with single and typical symptoms, remove excess old leaves and other diseased fruits, and leave diseased fruits with typical symptoms and healthy and normal fruits. Complete specimens with leaves, fruit branches, fruit stalks, fruit pedicles, diseased fruits, and healthy fruits. After pruning, clean the complete specimen with clean water, soak it in 70% alcohol for 3-4 minutes, and wash it with distilled water 3-4 times.
[0030] 2) The color is fixed. Put the sterilized and cleaned tomato late blight specimens into the fixative. The specimens occupy no more than 80% of the space of the fixative, and the specimens should be completely immersed in the fixa...
Embodiment 3
[0037] The red and yellow two-color plant disease specimen primary color preservation method of the present invention is used for the preservation of red and yellow sweet pepper anthracnose, and the specific steps are:
[0038] 1) Collection, sorting and sterilization of sweet pepper anthracnose specimens. Collect 7-8 ripe red and yellow bell pepper anthracnose diseased fruits with single and typical symptoms, select the diseased fruits with typical symptoms and healthy and normal fruits, with leaves, fruit stalks, fruit pedicles, diseased fruits, and healthy fruits complete specimen. After pruning, clean the complete specimen with clean water, soak it in 70% alcohol for 3-4 minutes, and wash it with distilled water 3-4 times.
[0039]2) The color is fixed. Put the sterilized and cleaned sweet pepper anthracnose specimens into the fixative solution. The specimen occupies no more than 80% of the space of the fixative solution, and the specimen should be completely immersed in...
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