The invention relates to a medical biological tissue structure and a preparation method and a special device of the medical biological tissue structure, and belongs to the technical field of biology, medical treatment and medical instruments. The medical biological tissue structure comprises a hollow pipe, a functional layer and a synthetic macromolecule protective film, wherein the hollow pipe is made of biological materials containing cells or being free of the cells, and the functional layer is attached to the hollow pipe and contains or does not contain the cells. The medical biological tissue structure, the preparation method and the special device are based on the attachment cross-linking and solidity principle, under control of a computer, the hollow pipe is firstly extruded out through a special spraying nozzle, then the biological materials are sprayed and attached to the hollow pipe to form the functional layer and accumulated layer by layer to form a composite molding body, and finally a synthetic macromolecule solution is sprayed to the outer surface of the composite molding body to form the protective film. According to the set molding steps, a three-dimensional structure body which contains synthetic macromolecule materials, the cells and the natural biological materials is finally manufactured, has the space complex shape and is of the gap structure is finally manufactured. The medical biological tissue structure can be molded at the normal temperature, the process is simple, the cell survival rate is high, distribution is even and controllable, and the good mechanical property and the good biological property are achieved.