Insect thin film virus feeding method and application thereof

A film-based technology for feeding poison and insects, applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of complex process, inconvenient, and inability to guarantee the rate of aphid transmission, and achieve the effect of simple and convenient operation and improved efficiency
CN113575518APending Publication Date: 2021-11-02INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
Publication Date
2021-11-02

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Abstract

The invention belongs to the technical field of wheat aphid physiology and phytopathology, and particularly relates to a piercing-sucking type insect thin film virus feeding method and application thereof. The method comprises the following steps: (1) uniformly thinning a plastic film, and covering the upper end of a container with openings at the two ends with the plastic film; (2) dropwise adding a virus feeding solution on the thin film; (3) uniformly thinning the plastic film, and covering the virus feeding solution with the plastic film; (4) inclining the opening at the lower end of the container upwards, and putting the insects into the container through the opening at the lower end; and (5) putting the container filled with the insects onto absorbent paper with the lower end opening facing downwards, and putting the container onto a plane for virus feeding. The method is simple and convenient to operate, effectively improves the efficiency of sucking food or virus solution by the insects, and provides convenience for further research on the virus transmission efficiency between the insects and plants.
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Description

technical field

[0001] The invention belongs to the technical field of wheat aphid physiology and plant pathology, and in particular relates to a method and application of piercing-sucking insect film feeding poison. Background technique

[0002] Barley Yellow Dwarf Viruses (BYDVs) can infect more than 150 kinds of cereal crops and grass weeds such as wheat, barley, rice, and oats. In my country, it can infect barley, wheat, rye, triticale, oats, wild oats, sweet corn, brome, golden foxtail, sagegrass, big ling grass, small teff, tufted wheat, silver scale grass and Golden foxtail, etc. Among them, spontaneous wheat seedlings, sansevieria, wild oats, golden foxtail, and small teff are the summer hosts of BYDVs. The yellow dwarf disease of wheat crops caused by it is one of the most serious viral diseases of cereal crops. The common symptoms after the plant is infected are dwarfing, yellowing, reddening and curling of leaves. The main symptom on wheat is leaf chlorosis, whi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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