88 results about "Phytosciara" patented technology

The invention discloses a phytophthora capsici LAMP (Loop-mediated isothermal Amplification) primer and a rapid detection method thereof, which are specially used for phytophthora capsici specific detection. The invention mainly designs a phytophthora capsici LAMP primer (F3, B3, FIP and BIP); the primer is subjected to isothermal amplification and a 50micromole/L Calcein-500micromole/L MnCl2 developing agent is added to develop or agarose gel electrophoresis detection is carried out, so as to observe green fluorescent light or a ladder belt with an LAMP characteristic. The phytophthora capsici LAMP primer and the rapid detection method thereof can be used for rapid, sensitive and accurate detection of plants infected by phytophthora capsici and the phytophthora capsici in the soil in production practice and can also be used for early diagnosis of field diseases and the monitoring and identifying of bacteria, so as to provide reliable technological and theoretical foundations for preventing and treating the diseases caused by the phytophthora capsici.

The invention provides a microbial agent. The microbial agent comprises the following components in parts by weight: 20 to 60 parts of hydrogen-oxidizing bacteria, 20 to 60 parts of nitrogen-fixing bacteria, 30 to 50 parts of actinomycetes and 30 to 60 parts of fungi, wherein the actinomycetes can be actinoplanes, streptomycetes or nocardia; and the fungi can be pythium or trichoderma. The microbial agent disclosed by the invention generates a certain synergistic effect through the combination of the hydrogen-oxidizing bacteria, the nitrogen-fixing bacteria, the actinomycetes and the fungi, and can improve the yield of wheat, promote the development of wheat root systems and improve the good earth holding capacity of roots for soil, so that main roots, side roots and fibrous roots are deeper and wider in coverage and are difficult to lodge and stronger in water absorption, eustipes close to the ground has better toughness and strength, the toughness and strength of the stems can be improved, and the stems are difficult to break. The microbial agent provided by the invention can shorten the growth and maturation time, enable crops to be matured in advance, reduce the risks of vigorous growth of seedlings and green remaining and late maturation of plants, and promote early fruiting and maturation of the plants to appear in the market.

The present invention provides a nucleic acid sequence comprising one or more selected from the group consisting of sequences SEQ ID NO: 1-7 and complementary sequences thereof, said nucleic acid sequence being derived from a plant, seed or cell comprising the maize event LP007-7, a representative sample of the seed comprising said event having been preserved with preservation number CCTCC NO: P202020. The transgenic maize event LP007-7 of the invention is resistant to ingestion impairment of lepidoptera pests and tolerant to phytotoxic effects of agricultural herbicides containing glyphosate. The corn plant with double characters has the following advantages: economic loss caused by lepidoptera pests is avoided; an agricultural herbicide containing glyphosate can be applied to corn crops; the corn yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking transgenic insertion fragments in a breeding population and a progeny thereof. Meanwhile, the detection method provided by the invention can be used for rapidly, accurately and stably identifying the existence of the plant material derived from the transgenic maize event LP007-7.

The invention discloses a paenibacillus polymyxa and application thereof in prevention and treatment of various soil-borne diseases. The preservation number of the paenibacillus polymyxa ZF129 protected by the invention is CGMCC (China General Microbiological Culture Collection Center) No.17631. The paenibacillus polymyxa ZF129 can remarkably inhibit the growth of pathogenic fungus hyphae, shows broad-spectrum antagonistic activity, and has the capability of promoting the growth of plants. The paenibacillus polymyxa ZF129 shows an excellent biocontrol character, has a further development potential, and lays a certain foundation for further study of the paenibacillus on the mechanism of disease prevention.

The invention provides a Fusarium oxyxporum f.sp. niveum type strain FON-GFP (Fusarium oxyxporum f.sp. niveum-green fluorescent protein) for expressing GFP as well as a preparation method and application of the strain. A fungal expression vector with GFP is provided and contains a homomycin B resistance gene (i) hph (/i) and a GFP gene (i) gfp (/i), the vector is converted into a Fusarium oxyxporum f.sp. niveum type strain race1 by adopting an agrobacterium-mediated method, a transformant can express the GFP, the converted strain is infected by a grafting rootstock and a root of citrullus lanatus respectively, and the infection condition of Fusarium oxyxporum f.sp. niveum type bacteria in tissues of a plant root and hypocotyls can be accurately analyzed by monitoring fluorescent strains in real time. The Fusarium oxyxporum f.sp. niveum type strain disclosed by the invention has the characteristics of quickly, simply and conveniently indicating the infection way of the Fusarium oxyxporum f.sp. niveum type bacteria in real time, and the method provided by the invention is suitable for various filamentous fungi and can be widely applied to scientific research fields.

The present invention belongs to the technical field of microorganisms and discloses a bacillus megaterium FJW1 biocontrol preparation, a preparation method and an application thereof. The bacterium strain is deposited in the China Center for Type Culture Collection and has a deposit number of CCTCC NO:M2019660. The bacillus megaterium has relatively good microorganism-inhibiting effects on a variety of phytopathogens. In field experiments, tomato plants are relatively good in growth by applying the strain of the bacillus megaterium. Height and width of the plants are significantly higher thanthose of the control group inoculated with only the pathogens. Due to the fact the bacillus megaterium secretes organic acids during a fermentation process, the bacillus megaterium is difficult for producing high-content spores during fermentation. Aiming at the fact that the a high-density fermentation method aiming at the strain is provided, the method breaks through the highest level of bacillus megaterium fermentation levels and bacterium spore number in the current market, the spore number is at least 10<10> CFU/ml when tank releasing is conducted.

The present invention provides a nucleic acid sequence comprising one or more selected from the group consisting of sequences SEQ ID NO: 1-7 and complementary sequences thereof, the nucleic acid sequence is derived from a plant, seed or cell comprising the corn event LP007-3, and a representative sample of the seed comprising said event is preserved with preservation number CCTCC NO: P202016. The transgenic maize event LP007-3 of the invention is resistant to ingestion impairment of lepidoptera pests and tolerant to phytotoxic effects of agricultural herbicides containing glyphosate. The corn plant with double characters has the following advantages: economic loss caused by lepidoptera pests is avoided; an agricultural herbicide containing glyphosate can be applied to corn crops; the corn yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking transgenic insertion fragments in a breeding population and a progeny thereof. Meanwhile, the detection method provided by the invention can be used for rapidly, accurately and stably identifying the existence of the plant material derived from the transgenic maize event LP007-3.

The invention provides a functional endophytic fungus for prompting the photosynthesis of eucalypt and application thereof. The functional endophytic fungus is Penicilliumsp. strain 7, wherein the preservation number of the strain 7 is CGMCCNo.4055. In the invention, the strain is obtained by separating and purifying from Guan'en eucalypt and eucalyptus urophydis, is inoculated on the eucalypt, is comprehensively judged according to each index of the photosynthesis so as to further prove that the functional endophytic fungus has the prompting function on the photosynthesis and has practical synergism on dry matters of the eucalypt, and the functional endophytic fungus capable of prompting the photosynthesis can be applied to the production. The strain has remarkable effect on prompting the photosynthesis of the plants and increasing the dry matter accumulation of the eucalypt; meanwhile, the invention has the remarkable effects on seedling tissue culture, radication and seedling hardening of the eucalypt.

The present invention provides a nucleic acid sequence comprising one or more selected from the group consisting of sequences SEQ ID NO: 1-7 and complementary sequences thereof, said nucleic acid sequence being derived from a plant, seed or cell comprising the corn event LP007-2, a representative sample of the seed comprising said event having been preserved with preservation number CCTCC NO: P202015. The transgenic maize event LP007-2 of the invention is resistant to ingestion impairment of lepidoptera pests and tolerant to phytotoxic effects of agricultural herbicides containing glyphosate. The corn plant with double traits has the following advantages: economic loss caused by lepidoptera pests is avoided; an agricultural herbicide containing glyphosate can be applied to corn crops; the corn yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking transgenic insertion fragments in a breeding population and a progeny thereof. Meanwhile, the detection method provided by the invention can be used for rapidly, accurately and stably identifying the existence of the plant material derived from the transgenic maize event LP007-2.

The invention relates to the technical field of plant genetic engineering, in particular to a cucumber glutaredoxin gene CsGRX4 and application thereof in susceptibility to botrytis cinerea. The invention discovers an infection of cucumber glutaredoxin gene CsGRX4 by corresponding pathogenic fungus botrytis cinerea, and the susceptibility to the pathogenic fungus botrytis cinerea can be obviously improved by overexpression of the cucumber glutaredoxin gene CsGRX4. After a botrytis cinerea spore suspension is quantitatively inoculated, the disease spot area of a transgenic line of CsGRX4 overexpression is obviously larger than that of a wild type control, and the result shows that overexpression of the cucumber glutaredoxin gene CsGRX4 in arabidopsis thaliana improves the susceptibility of a transgenic plant (the arabidopsis thaliana) to the pathogenic fungus botrytis cinerea.

The invention discloses a portable arbuscular mycorrhizal fungus application method, and relates to the field of microbial fertilizers. A propagated plant with arbuscular mycorrhizal fungus spores and a propagated matrix rich in arbuscular mycorrhizal fungus spores are processed into a degradable capsule preparation, and then the degradable capsule preparation is applied into soil. According to the invention, the conventional arbuscular mycorrhizal fungus application modes such as broadcast sowing, furrow application, coating and injection in the technical field are changed, and the fungus is prepared into the capsule preparation to be applied, so that the fungus can survive in a more suitable small environment, the survival rate of the fungus is improved, the infection effect on plants is improved, the operation of the capsule preparation is simpler and more convenient, and the application cost and the transportation cost are reduced. According to the invention, mechanical production of the microbial capsule preparation is realized, large-scale agricultural and forestry mechanical operation is facilitated, and the universality is wide.

The invention relates to an experimental method for measuring indoor control effect on soybean phytophthora root rot caused by chemical agents. Soybean phytophthora root rot is of foreign quarantine pests of China and belongs to typical soil-borne disease. Literature reports that the soybean phytophthora root rot develops by 3 times each year and threatens soybean production of China. At present, an irrigation bacterial liquid bacterial inoculation method, a hypocotyledonary axis wound inoculation method, a hypocotyledonary axis zoospore inoculation method and a bacterial layer inoculation method are generally adopted for measuring potted living bodies with the soybean phytophthora root rot by the chemical agent in a lab, but the four experimental methods have certain defects, for example, the concentration of obtained zoospore suspensions cannot be kept consistent; the inoculation speed is low, the workload is high, and the death of a large quantity of diseased plants is easily caused; zoospores generated by different phytophthora sojae plants are different in number and size; the germination rate of soybean seeds is low and the rot, dead seedlings and the like of the soybean seeds are easily caused. The experimental method adopted by the invention is simple to operate, soybeans can be uniformly infected with phytophthora sojae, the control morbidity is uniform and stable and is kept at 85%-95%, the difference between a chemical agent applying region and a control region can be well distinguished, and the symptoms of disease can be simply and quickly observed.

The invention provides a biocontrol fungus identification method of soft rot diseases of boilen taros, and relates to the technical field of biological control. The biocontrol fungus identification method of soft rot diseases of boilen taros comprises the steps of preparing experiment materials, performing separation and purification on fungi, performing in vitro stem tuber returning and screeningtest, observing the shape of a bacterial strain, performing strain molecular identification, screening the growth characteristics of the bacterial strain, and performing field living body control effect test on the strain plants. 22 fungi obtained by separation and purification from Fuding boilen taro rhizosphere soil and healthy plant corms are identified, and in vitro returning and field livingbody control effect test is used for identifying the biocontrol effect of boilen taro bacterial strains. A molecule sequencing method is adopted for screening independent unduplicated bacterial strains for performing conventional shape characteristic and physiological and biochemical test measurement, and the best screening method is researched, so that a research reference is provided for biological control of the soft rot diseases of the boilen taros in the future, and the method is worth of being widely popularized.

The invention relates to the technical field of pesticides, in particular to a cinnamyl aldehyde-containing pesticide composition as well as a preparation method and application thereof. The pesticidecomposition disclosed by the invention improves the problem of drug resistance of an existing nematicide, has equivalent root-knot nematode activity after treatment for 1 day, cause no recovery phenomenon of root-knot nematode as time goes on, has high safety to plants, can be applied in the whole growth period of the plants, and can effectively prevent and remove soil-borne diseases. The inventor accidentally finds that a component A and a component B of the pesticide composition have a remarkable synergistic effect, the cotoxicity coefficient of the composition is 200 or above, the pesticide effect is improved by 2-3 times, and the pesticide composition has a remarkable synergistic effect. Moreover, the pesticide composition disclosed by the invention also improves the problems of strong smell, no environmental protection, phytotoxicity and the like of a medicament, and is suitable for a wider range of crops.

The invention belongs to the technical field of porous fungus cultivation, and discloses a system and a method for large-scale production of a wild porous fungus through a biological fermentation technology. The system for large-scale production of the wild porous fungus through the biological fermentation technology comprises a temperature monitoring module, a humidity monitoring module, a central control module, a data summarization module, a porous fungus information acquisition module, a comparative analysis module, a cultivation area determination module, a porous fungus selection module, a biological fermentation module and a porous fungus cultivation module. According to the system and method for large-scale production of the wild porous fungus through the biological fermentation technology, by monitoring the temperature and humidity of the field environment, the temperature and humidity information of the area in the growth cycle of the porous fungus is achieved, then the area matched with the temperature and humidity needed by the growth of the porous fungus is selected for producing the porous fungus, cultivation of the porous fungus can be achieved, the survival rate is increased, and the reproduction rate is increased; the porous fungus is cultivated on a large scale, dead leaves in the area can be decomposed, and more nutrients are provided for plants such as trees and the like.

The invention discloses a phytophthora colocasiae LAMP (loop-mediated isothermal amplification) detection primer and it rapid detection method, which can be used for the specific detection of the phytophthora colocasiae. With the phytophthora colocasiae LAMP detection primer, via isothermal amplification, green fluorescence light or trapezoidal bands with LAMP characteristics can be observed by color developing of SYBR-green-I color developing agent or electrophoretic detection of agarose gel. The LAMP primer and the detection method of the invention have high accuracy, specificity, convenience in operation and good practicability, and realize the constant temperature amplification, which provides a new technology platform for the detection of phytophthora colocasiae, can be used for quickly, flexibly and accurately detecting of infected plants of phytophthora colocasiae, and phytophthora colocasiae of the initial infection stage in the production practice and for the early diagnosis, monitoring and identification of the crop diseases, and can provide reliable technical and theoretical basis for the prevention and control of the diseases.

The invention belongs to the technical field of wheat aphid physiology and phytopathology, and particularly relates to a piercing-sucking type insect thin film virus feeding method and application thereof. The method comprises the following steps: (1) uniformly thinning a plastic film, and covering the upper end of a container with openings at the two ends with the plastic film; (2) dropwise adding a virus feeding solution on the thin film; (3) uniformly thinning the plastic film, and covering the virus feeding solution with the plastic film; (4) inclining the opening at the lower end of the container upwards, and putting the insects into the container through the opening at the lower end; and (5) putting the container filled with the insects onto absorbent paper with the lower end opening facing downwards, and putting the container onto a plane for virus feeding. The method is simple and convenient to operate, effectively improves the efficiency of sucking food or virus solution by the insects, and provides convenience for further research on the virus transmission efficiency between the insects and plants.

The invention belongs to the technical field of microbial application, and provides a biocontrol fungicide for preventing and treating the potato bacterial wilt. According to the invention, the content of viable bacteria in each gram of the biocontrol fungicide is no less than 1 * 10<8> cfu, and active ingredients of the biocontrol fungicide are formed by compounding two selected from the group consisting of bacillus subtilis, bacillus licheniformis, actinomycetes and bacillus pumilus. The biocontrol fungicide provided by the invention can improve the activity of part of defensive enzymes of plants, influence the change of microbial community structures in rhizosphere soil and enhance the systematic resistance of the plants to bacterial wilt, and has good biocontrol effect.