77 results about "Phytosciara" patented technology

The present invention discloses a gene, GmCDK8, that negatively regulates soybean resistance to Phytophthora spp., and its applications. The disclosed GmCDK8 knockout soybean plants exhibit enhanced resistance to the fungus. Specifically, the results of the soybean cultivar "Williams 82," which lacks GmCDK8, under Phytophthora stress, including root lesion length, leaf lesion area, Phytophthora accumulation, and infected necrotic area on leaves, indicate that knocking out GmCDK8 significantly improves plant resistance to Phytophthora spp. Genetic engineering of this gene for plant resistance to Phytophthora root rot not only provides an important genetic resource for plant molecular breeding but also offers an effective means for achieving high plant yields.

The invention discloses a Chinese chestnut composite phosphate-solubilizing bacterial agent and application thereof, and belongs to the technical field of microorganisms. The invention provides a compound phosphate-solubilizing bacterial agent. The compound phosphate-solubilizing bacterial agent is prepared from phosphate-solubilizing bacteria GXLK-W6 and phosphate-solubilizing fungi GXLK-WZ1-1. The phosphorus-solubilizing bacteria GXLK-WZ1-1 are classified and named as Aspergillus niger, the preservation number of the bacteria is CGMCC (China General Microbiological Culture Collection Center) No.41390, the phosphorus-solubilizing bacteria GXLK-W6 are classified as Burkholderia pyrrolothrix, and the preservation number of the bacteria is CGMCC No.31124. The phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1 and the phosphorus-solubilizing bacteria GXLK-WZ1-1 and The strains are independently separated from rhizosphere soil of Chinese chestnuts and are screened. And calcium phytate is added into the microbial inoculum to serve as a slow-release phosphorus source. The compound phosphate-solubilizing bacterial agent improves the growth amplification of Chinese chestnut seedlings, promotes the growth of root systems, and promotes the phosphorus absorption of plants. The compound phosphate-solubilizing bacterial agent can improve the dissolution efficiency of inorganic phosphorus in soil and increase the content of soluble available phosphorus, and plays an important role in improving the fertility of the soil.

The invention discloses a sweet potato arbuscular disease phytoplasma TaqMan fluorescent quantitative detection primer, a kit and a detection method, the primer and a probe group are specially used for specific detection of sweet potato arbuscular disease phytoplasma; the invention also designs a fluorescence quantification (QPCR) kit based on the primer probe group. The detection primer group is as follows: a forward primer F as shown in SEQ ID No: 1, a reverse primer R as shown in SEQ ID No: 2, and a probe primer Probe as shown in SEQ ID No: 3; the sweet potato arbuscular disease phytoplasma can be specifically amplified within 1 hour by extracting the DNA of the sweet potato plant infected with the phytoplasma and carrying out QPCR, and the detection sensitivity is 10 copies / uL. According to the TaqMan QPCR detection technology established for the sweet potato arbuscular disease phytoplasma, the problems that an existing sweet potato arbuscular disease phytoplasma detection method is time-consuming and labor-consuming, poor in specificity and sensitivity, easy to cause pollution and the like are solved, and a technical basis is provided for early diagnosis of the sweet potato arbuscular disease phytoplasma.

The invention discloses a gene GmCDK8 for negative regulation and control of phytophthora sojae resistance and application of the gene GmCDK8. GmCDK8-knocked-out soybean plants disclosed by the invention enhance the resistance of soybeans to phytophthora sojae; the results of root scab length, leaf scab area, phytophthora accumulation amount, leaf infected and necrotic areas and the like of the soybean cultivar Williams 82 without the GmCDK8 under the stress of phytophthora show that the resistance of the plant to the phytophthora can be remarkably improved by knocking out the GmCDK8. Genetic engineering improvement of plant phytophthora root rot resistance is carried out through the gene, important gene resources are provided for plant molecular breeding, and an effective means is provided for high yield of plants.

The invention relates to a potassium-dissolving bacterial agent containing yellow microspora fungi, a seed coating solution and application of the potassium-dissolving bacterial agent and the seed coating solution, and belongs to the technical field of microorganisms. In order to solve the technical blank of the yellow microspora fungi in the aspects of potassium dissolving and kidney bean growth promotion in the prior art, the invention provides the potassium-dissolving microbial inoculum containing the yellow microspora fungi, the yellow microspora fungi are respectively yellow microspora landrace and yellow microspora Xing'an, and the potassium-dissolving microbial inoculum has better potassium-dissolving activity and can be used for preparing the potassium-dissolving microbial inoculum containing the yellow microspora fungi. Symbiosis with plant root systems can be achieved, and plant growth and development are promoted; the kidney bean seed coating liquid containing the potassium bacteria agent can promote accumulation of biomass of kidney bean plants, the setting rate of kidney beans is increased, and the yield of the kidney beans is increased; when the spore concentration is 1 * 10 < 6 > / mL to 1 * 10 < 8 > / mL, the seed coating solution has an obvious and stable growth promoting effect on kidney beans. The potassium-dissolving bacterial agent and the seed coating solution provided by the invention can be applied to soil potassium dissolving and promotion of kidney bean growth.

Provided is a stabilized aqueous hydrogen peroxide composition comprising a sugar alcohol that does not contain silver, organophosphonate, peroxyacetic acid or other peracid, or stannate. The composition optionally includes a carboxylic acid. The composition is suitable as a disinfectant and oxidizer, and for the treatment, inhibition, or eradication of fungi or nematodes. Also provided is a method for reversing a bacterial or fungal infection in an infected plant, and a method for enhancing plant growth and / or productivity. Also provided is a method for inhibiting or preventing fungal spore germination in soil in a fungal infested area. Also provide is a method of controlling or preventing nematode infestation of a plant, and a method of reversing damage of a plant caused by nematode infestation. Also provided is a method for treating or reversing Panama disease in an infected banana plant.

The invention discloses a specific detection target PsRrp8 of phytophthora sojae, a DNA (deoxyribonucleic acid) sequence of the specific detection target PsRrp8 is shown as SEQ ID NO.1. The invention further provides a primer combination for detecting the phytophthora sojae, the primer combination comprises a forward primer and a reverse primer, the sequence of the forward primer PsRrp8-qPCR-F1 is shown as SEQ ID NO.2, and the sequence of the reverse primer PsRrp8-qPCR-R1 is shown as SEQ ID NO.3. The invention further provides a kit for detecting the phytophthora sojae. The lowest detectable DNA concentration of the detection primer combination provided by the invention on the phytophthora sojae (P.sojae) is 0.332 pg.L <-1 >, which indicates that the quantitative detection technology has high sensitivity. The method can be successfully applied to phytophthora detection of phytophthora root rot disease field plants, phytophthora sojae can be rapidly detected, and a new technical means is provided for early warning of diseases.

The invention relates to the technical field of microbial engineering, and particularly discloses bacillus velezensis MSGL-ZJ1 as well as application and a microbial preparation thereof. The bacillus velezensis MSGL-ZJ1 is preserved in the China General Microbiological Culture Collection Center on September 30, 2024, and the preservation number of the bacillus velezensis MSGL-ZJ1 is CGMCC (China General Microbiological Culture Collection Center) NO.32125. The bacillus velezensis MSGL-ZJ1 is named as bacillus velezensis MSGL-ZJ1. The bacillus velezensis MSGL-ZJ1 and the fermentation liquor thereof are applied to preparation of products for inhibiting microorganisms or / and promoting plant growth. The microbial composition is prepared from the bacillus velezensis MSGL-ZJ1, and further comprises the bacillus velezensis MSGL-ZJ1. The bacillus velezensis MSGL-ZJ1 and the microbial composition provided by the invention can effectively inhibit the growth of phytopathogens, can be used as a broad-spectrum antibacterial biocontrol inoculant, and can effectively promote the growth and development of plants and enhance the stress resistance of the plants.

The invention provides application of a CaCRY2 gene in regulating and controlling resistance of plants to phytophthora capsici, and belongs to the technical field of plant molecular biology and plant genetic engineering. The gDNA sequence of the CaCRY2 gene is as shown in SEQ ID NO: 1, and the cDNA sequence of the CaCRY2 gene is as shown in SEQ ID NO: 2; the expression of the CaCRY2 gene is inhibited, and the resistance of the plant to phytophthora capsici is enhanced; the overexpression of the CaCRY2 gene weakens the resistance of the plant to phytophthora capsici, and the plant is a solanaceae plant. The invention provides a capsicum phytophthora blight related gene CaCRY2 and application thereof. The resistance of capsicum plants to phytophthora blight can be enhanced by silencing the CaCRY2 gene through a virus-induced gene silencing technology; the resistance of the tobacco plants to epidemic diseases can be weakened by overexpressing the CaCRY2 gene in the tobacco plants through a transgenic technology; the invention provides a favorable basis for improving the disease resistance of plants and cultivating disease-resistant varieties.

The invention relates to a trichoderma asperellum and pseudomonas aeruginosa compound microbial agent as well as a preparation method and application thereof. The compound microbial agent is prepared from a pseudomonas aeruginosa solid fermentation product, a trichoderma asperellum solid fermentation product, an auxiliary agent and a synergist, the number of effective spores in the compound microbial agent is greater than 10 < 10 > CFU / g; the pseudomonas aeruginosa is preserved in the China General Microbiological Culture Collection Center (CGMCC) on September 13, 2022, and the preservation number of the pseudomonas aeruginosa is CGMCC No.25697; the trichoderma asperellum is preserved in the China General Microbiological Culture Collection Center (CGMCC) on August 9, 2023, and the preservation number of the trichoderma asperellum is CGMCC No. 40752. The compound microbial agent can effectively solve the problems of soil-borne diseases such as pepper phytophthora blight and continuous cropping obstacles in the prior art, and has an obvious growth promoting effect on melon and vegetable plants such as pepper.

The invention belongs to the technical field of gene engineering, and relates to application of glutaredoxin StGRX5 in plant disease resistance. The invention provides an application of glutaredoxin StGRX5 in the disease resistance of plants. The glutaredoxin StGRX5 is silenced in potato plants, and the resistance of the potato plants to phytophthora infestans can be enhanced.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention provides a phytophthora nicotianae biocontrol actinomycete FK-1 and application thereof, and belongs to the technical field of biocontrol bacteria. The invention provides a streptomyces strain FK-1, and the preservation number of the streptomyces strain FK-1 is CCTCC (China Center for Type Culture Collection) NO: M 20242872. The streptomyces FK-1 disclosed by the invention shows remarkable broad-spectrum antibacterial activity on various phytopathogens, and has the capabilities of slowing down pathogen infection and reducing plant diseases. The inhibition rate of the streptomyces FK-1 to phytophthora nicotianae reaches 82.08%, the antibacterial activity of strain fermentation liquor can reach 85.88%, and the prevention and treatment effect of indoor potting is 80.65%; meanwhile, the phytophthora nicotianae hyphae can be deformed, wound and irregularly bent, so that the growth of the phytophthora nicotianae hyphae is inhibited, and the method can be applied to prevention and control of phytophthora nicotianae.

The invention discloses a novel application of massinia phosphate solubilizing C22 in prevention and treatment of fungal diseases of plants, and belongs to the technical field of agricultural biology, and particularly relates to a novel application of massinia phosphate solubilizing C22 in prevention and treatment of fungal diseases of plants. The known strain C22 has a remarkable antagonistic effect on pathogenic bacteria fusarium pseudograminearum of wheat stem rot, and the plate inhibition rate reaches 36.79%. Potting and field tests prove that wheat basal stem rot can be effectively prevented and treated by applying the C22 fungicide, the morbidity in the seedling stage is reduced by 21.64%, the morbidity in the adult-plant stage is reduced by 55.06%, the DON toxin content in plants can be remarkably reduced by 68.26%, and the pathogenic bacterium load can be remarkably reduced. According to the invention, the potential of the phosphorus-solubilizing massinia sp. C22 in the aspect of biological control is disclosed for the first time, and a new core microbial resource and a technical scheme are provided for developing a green biological pesticide for controlling wheat stem rot.

The invention discloses application of alfalfa VIK protein in cultivation of phytophthora root rot resistant plants, and belongs to the technical field of biology. The technical problem to be solved by the invention is how to improve the disease resistance of plants. The VIK protein disclosed by the invention is a protein of which the amino acid sequence is SEQ ID No. 1 or SEQ ID No. 4. Experiments prove that after the phytophthora phytopathogen is inoculated, compared with a wild plant, the mutant obtained by editing the VIK coding gene has the advantages that the disease resistance is enhanced, the survival rate is improved, the fresh weight, the overground part height, the root length and the plant height of the plant are increased, and the relative conductivity of leaves is reduced. Therefore, the resistance of the plant to the phytophthora phytopathogen is improved through the mutation of the VIK protein and the coding gene thereof, the coding gene of the VIK can be targeted through a gene editing technology, a VIK gene knockout plant is obtained, and a plant resisting the phytophthora phytopathogen is cultivated.

The invention relates to the technical field of microorganisms, and discloses brevibacillus megatherium, a fungicide containing the brevibacillus megatherium, application of the brevibacillus megatherium and the fungicide and a method for preventing and treating plant diseases. The preservation number of the brevibacillus formmosus is CGMCC (China General Microbiological Culture Collection Center) No.32491, and the brevibacillus formmosus can be used for preparing the bacillus formmosus. The invention also provides a method for preventing and treating plant diseases. The method comprises the step of irrigating plants by using the brevibacillus beautiful and the fungicide. The brevibacillus megaterium has the effects of promoting growth and preventing diseases, has a relatively high bacteriostasis rate on phytophthora nicotianae, can efficiently antagonize black shank caused by phytophthora nicotianae, and has relatively strong capability of secreting auxin and exopolysaccharide.

The invention relates to the technical field of gene engineering, in particular to a cynanchum thesioides CtPDS gene as well as a VIGS virus vector and a silencing method of the cynanchum thesioides CtPDS gene. The invention provides phytoene dehydrogenase of cynanchum thesioides. The phytoene dehydrogenase has an amino acid sequence as shown in SEQ ID NO. 1. The invention discloses an amino acid sequence of cynanchum thesioides phytoene dehydrogenase and a sequence of a coding gene CtPDS of the cynanchum thesioides phytoene dehydrogenase, and provides a target nucleic acid molecule for silencing the CtPDS gene, a VIGS silencing system and a silencing method on the basis of the amino acid sequence of the cynanchum thesioides phytoene dehydrogenase. The leaf CtPDS gene expression level of a CtPDS gene silent plant constructed by the method provided by the invention is remarkably reduced, the silence efficiency reaches 56-65%, the chlorophyll and carotenoid contents are remarkably reduced, the leaf has a whitening phenotype, and a technical support is provided for developing functional research of cynanchum thesioides key genes.

The invention discloses a novel leuconostoc screening method and an antibacterial mechanism of leuconostoc on leaf mold of zanthoxylum bungeanum, and relates to the technical field of pathogenic bacteria experiment and analysis. The novel leuconostoc screening method comprises the following steps that S1, materials are prepared, specifically, rotten Chinese prickly ash tree plants are selected, and tested strains in the Chinese prickly ash tree plants comprise plant pathogens of alternaria alternata, fusarium solani, fusarium layered, fusarium oxysporum, fusarium syphilis and colletotrichum pini, and are stored in a refrigerator of a laboratory at the temperature of 4 DEG C for standby application; in the invention, the leuconostoc also has an efficient inhibition effect on field crop pathogenic fungus alternaria alternata, and the research expands the biocontrol spectrum of lactic acid bacteria. Meanwhile, the invention discloses a molecular mechanism that the Leuconostoc suionicum HJ-L synergistically regulates and controls the synthesis of antibacterial substances through lipid metabolism, an ABC transport system and an autophagy pathway, and a theoretical basis and a practical basis are provided for the application of the strain in green prevention and control of the leaf mold of Chinese prickly ash.

The present application belongs to the field of microbial technology, and particularly relates to a fungus in promoting growth of Nauclea officinalis and increasing yield of active substance in a microbial agent. The fungus has a preservation number of GDMCC No: 66543, and belongs to Cladosporium herbarum Cladosporium tenuissimum. Researches show that the fungus can well colonize in the roots of Nauclea officinalis, promote growth of the plant, and increase the yield of vinosamycin lactam in the leaves of Nauclea officinalis, thereby facilitating further improvement of availability of medicinal substance vinosamycin lactam and promoting cultivation of Nauclea officinalis.

The invention belongs to the technical field of plant disease control, and particularly relates to application of amide compounds or agrochemically acceptable salts thereof in preparation of antifungal drugs. The amide compound or the acceptable salt of the amide compound shows remarkable broad-spectrum antibacterial activity on various plant pathogenic fungi such as rhizoctonia solani, tobacco target leaf spot bacteria and magnaporthe oryzae, and has no visible phytotoxicity symptom on plants in an effective concentration range. The action mechanism research shows that the amide compound or the acceptable salt thereof inhibits the growth of fungi through multiple ways: (1) the biological wall and cell membrane structures of the fungi are destroyed to cause the outflow of contents so as to play a bactericidal role; (2) the physiological function of fungal normal cells is interfered, so as to induce programmed death of fungal cells. The invention provides an important reference way for developing a high-efficiency novel agricultural bactericide.

The utility model particularly discloses an insect receiving experiment device for phytophagous insects, which comprises a shell, a shielding device and a plurality of supporting rods, the supporting rods comprise a first gauze element and a second gauze element, and the first gauze element wraps the outer side of the top of a plant and is used for receiving the phytophagous insects; the second gauze element is connected with the outer side of the bottom end of the first gauze element, is arranged in the middle of the plant and is hollow; the shielding device is formed by detachably connecting two halves and arranged on the upper surface of the plant pot body, the upper surface of the shielding device is fixedly connected with the bottom end of the shell, and a through hole allowing a plant stem to penetrate through is formed in the middle of the shielding device; the supporting rods are fixedly connected to the periphery of the shell, and the bottoms of the supporting rods are fixedly connected with the shielding device. According to the utility model, mechanical damage to plants can be reduced.

The invention belongs to the field of marine natural product chemistry, and particularly relates to marine fungi and application thereof in prevention and treatment of pepper phytophthora blight. The marine fungus crude extract disclosed by the invention shows very strong antifungal activity on phytophthora capsici under the concentration of 300 mu g / mL. 13 monomeric compounds are separated and identified, Territrem B shows a remarkable antifungal effect on phytophthora capsici, the EC50 value is 22.96 mu g / mL, mycelial growth, sporangium formation and zoospore generation of the phytophthora capsici can be effectively inhibited, and the defense capacity of pepper plants is enhanced; the form of the phytophthora capsici mycelium is changed, and the integrity of a cell membrane is destroyed. In addition, Territrem B inhibits the activity of antioxidant enzyme, reduces the content of soluble protein and increases the MDA level, thereby inhibiting the normal growth of phytophthora capsici.

The invention discloses a PpbZIP1 gene and a PpbZIP18 gene of erencigia parviflora and application of the PpbZIP1 gene and the PpbZIP18 gene, and belongs to the technical field of gene engineering. The CDS sequence of the PpbZIP1 gene is as shown in SEQ ID NO. 1; 1, and the CDS sequence of the PpbZIP18 gene is as shown in SEQ ID NO. 2. According to the invention, by cloning and analyzing the PpbZIP1 and PpbZIP18 genes of Eriphaga parviflora and combining a heterologous overexpression technology to verify the functions of the genes on arabidopsis thaliana, the overexpression of the PpbZIP1 or PpbZIP18 genes can significantly improve the tolerance of plants to drought stress. The invention provides a theoretical basis and candidate gene resources for developing drought-tolerant crops.

The invention discloses bacillus siamensis and application thereof, and relates to the technical field of microorganisms. The strain is bacillus siamensis LYQ15 which is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 14, 2024, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO: 30024. The strain provided by the invention can be used for effectively inhibiting the growth and reproduction of phytophthora and pseudomonas solanacearum as well as related diseases caused by the growth and reproduction of the phytophthora and the pseudomonas solanacearum; in addition, the bacillus siamensis LYQ15 can inhibit reproduction of pathogenic bacteria and epidemic of diseases in an environment with the pathogenic bacteria, and healthy growth of tobacco plants is guaranteed.

This invention relates to the field of agricultural microbiology, and in particular to a strain of *Bacillus erythropoietinus* KF-B6 and its applications, especially in the control of continuous cropping obstacles in apple orchards. The *Bacillus erythropoietinus* KF-B6, with preservation number CGMCC No. 32131, possesses a complex set of functions including rapid and high EPS (explosive peroxide) production, high chitinase production, production of water-soluble and volatile antibacterial substances, and decomposition of macromolecular organic matter. The application of this bacterium, its microbial agents, and compound microbial fertilizers can effectively increase the number of rhizosphere soil bacteria, reduce the number of pathogenic fungi, increase the ratio of bacteria to fungi, and promote the recovery of the soil microbial ecosystem in apple orchards with continuous cropping obstacles; increase the proportion of water-stable aggregates, increase soil organic matter content, and increase soil enzyme activity, thus improving the soil; improve the survival rate of saplings planted during the renewal of new varieties in old orchards with continuous cropping obstacles, promote root vitality and plant growth, and comprehensively alleviate and control continuous cropping obstacles in apple orchards.

The invention discloses an application of PbGAB2 in prevention and control of rape clubroot, a G protein coupled receptor PbGAB2 with high expression level in each stage is screened out from a plasmodiophora brassicae ZJ-1 transcriptome, an RNAi vector of the gene is constructed, a corresponding transgenic plant is obtained, and the application of PbGAB2 in prevention and control of rape clubroot is realized. A disease resistance test of plasmodiophora brassicae shows that the PbGAB2-RNAi transgenic line disclosed by the invention shows excellent disease resistance phenotype, and the G protein coupled receptor PbGAB2 of the plasmodiophora brassicae can be used as a gene resource for resisting clubroot.

This invention provides the application of Evodia rutaecarpa root extract in the preparation of antifungal agents, belonging to the field of agricultural fungal control technology. The active ingredient of the Evodia rutaecarpa root extract is naturally derived and easily obtained; its unique chemical structure and novel mechanism of action effectively avoid the problem of pathogen resistance caused by the concentrated mechanisms of action of existing fungicides, filling the market gap for quinolone-based plant-derived pesticides. The Evodia rutaecarpa root extract exhibits a precise antifungal spectrum and significant inhibitory effect, showing obvious inhibitory activity against soil-borne and interplant-borne plant fungi that are serious threats in agricultural production, such as Phytophthora tobaccoii, Fusarium equisetifolium, Colletotrichum gloeosporioides, and Fusarium moniliforme.

The application discloses a primer composition for detecting Pythium sylvesterianum based on LAMP technology and application thereof, and belongs to the technical field of microorganism detection; the primer composition is a LAMP primer composition designed according to a specific sequence shown in SEQ ID NO. 1. The application adopts a method for rapidly detecting Pythium sylvesterianum, the pathogenic bacterium of stem base rot of raw ginger, based on loop-mediated isothermal amplification technology, and establishes a perfect detection and field real-time detection system for soil samples, water source samples and diseased plant samples, thereby opening up a new train of thought and method for the measurement and prediction of field crop diseases, winning valuable time for the prevention and control of related diseases, and reducing the loss caused by diseases to a certain extent.

This invention discloses the application of reducing the expression of negatively regulated genes in improving resistance to Phytophthora sojae, belonging to the field of plant genetic engineering. Research has found that the soybean genes GmTIFY5a or GmTIFY5b have a negative regulatory function on resistance to Phytophthora sojae. Knocking out GmTIFY5a or GmTIFY5b, or a combination of GmTIFY5a and GmTIFY5b, can enhance soybean resistance to Phytophthora sojae. This can be applied to improving soybean resistance to diseases caused by Phytophthora sojae or to breeding soybean varieties resistant to these diseases. In the soybean cultivar "Williams 82," a double mutant lacking both GmTIFY5a and GmTIFY5b, the accumulation of Phytophthora sojae was reduced by 50-60% compared to the wild type, the lesion infection area was reduced by 80-90%, and the survival rate was increased by 20-30%. Knocking out GmTIFY5a and GmTIFY5b significantly improved plant resistance to Phytophthora sojae. By genetically engineering the above genes to combat plant root rot caused by septicemia, we can not only provide important genetic resources for plant molecular breeding, but also provide an effective means to achieve high plant yields.

The application belongs to the technical field of bio-agriculture, and particularly relates to a gene MOS4 and application of an encoded protein thereof in regulation of plant resistance to parasitic Phytophthora. MOS4 Through gene function research, it is found that overexpression of the gene MOS4 significantly improves the plant resistance to parasitic Phytophthora, and that the expression of the gene MOS4 weakens the plant resistance to parasitic Phytophthora, and that the gene MOS4 positively regulates the plant resistance to parasitic Phytophthora. Further immune mechanism research finds that the gene MOS4 regulates the related genes of a salicylic acid (SA) signal path, interacts with a transcription factor ERF019, and jointly activates the plant immune response. In addition, a dual luciferase experiment proves that the gene MOS4 and the gene ERF019 cooperatively regulate the transcription of PR1. The application provides a new molecular target for prevention and treatment of plant diseases caused by parasitic Phytophthora, and has important agricultural application value.