31 results about "Phytosciara" patented technology

The invention belongs to the technical field of gene engineering, and relates to application of glutaredoxin StGRX5 in plant disease resistance. The invention provides an application of glutaredoxin StGRX5 in the disease resistance of plants. The glutaredoxin StGRX5 is silenced in potato plants, and the resistance of the potato plants to phytophthora infestans can be enhanced.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention discloses a novel application of massinia phosphate solubilizing C22 in prevention and treatment of fungal diseases of plants, and belongs to the technical field of agricultural biology, and particularly relates to a novel application of massinia phosphate solubilizing C22 in prevention and treatment of fungal diseases of plants. The known strain C22 has a remarkable antagonistic effect on pathogenic bacteria fusarium pseudograminearum of wheat stem rot, and the plate inhibition rate reaches 36.79%. Potting and field tests prove that wheat basal stem rot can be effectively prevented and treated by applying the C22 fungicide, the morbidity in the seedling stage is reduced by 21.64%, the morbidity in the adult-plant stage is reduced by 55.06%, the DON toxin content in plants can be remarkably reduced by 68.26%, and the pathogenic bacterium load can be remarkably reduced. According to the invention, the potential of the phosphorus-solubilizing massinia sp. C22 in the aspect of biological control is disclosed for the first time, and a new core microbial resource and a technical scheme are provided for developing a green biological pesticide for controlling wheat stem rot.

The invention discloses a novel leuconostoc screening method and an antibacterial mechanism of leuconostoc on leaf mold of zanthoxylum bungeanum, and relates to the technical field of pathogenic bacteria experiment and analysis. The novel leuconostoc screening method comprises the following steps that S1, materials are prepared, specifically, rotten Chinese prickly ash tree plants are selected, and tested strains in the Chinese prickly ash tree plants comprise plant pathogens of alternaria alternata, fusarium solani, fusarium layered, fusarium oxysporum, fusarium syphilis and colletotrichum pini, and are stored in a refrigerator of a laboratory at the temperature of 4 DEG C for standby application; in the invention, the leuconostoc also has an efficient inhibition effect on field crop pathogenic fungus alternaria alternata, and the research expands the biocontrol spectrum of lactic acid bacteria. Meanwhile, the invention discloses a molecular mechanism that the Leuconostoc suionicum HJ-L synergistically regulates and controls the synthesis of antibacterial substances through lipid metabolism, an ABC transport system and an autophagy pathway, and a theoretical basis and a practical basis are provided for the application of the strain in green prevention and control of the leaf mold of Chinese prickly ash.

The present application belongs to the field of microbial technology, and particularly relates to a fungus in promoting growth of Nauclea officinalis and increasing yield of active substance in a microbial agent. The fungus has a preservation number of GDMCC No: 66543, and belongs to Cladosporium herbarum Cladosporium tenuissimum. Researches show that the fungus can well colonize in the roots of Nauclea officinalis, promote growth of the plant, and increase the yield of vinosamycin lactam in the leaves of Nauclea officinalis, thereby facilitating further improvement of availability of medicinal substance vinosamycin lactam and promoting cultivation of Nauclea officinalis.

The invention belongs to the field of marine natural product chemistry, and particularly relates to marine fungi and application thereof in prevention and treatment of pepper phytophthora blight. The marine fungus crude extract disclosed by the invention shows very strong antifungal activity on phytophthora capsici under the concentration of 300 mu g / mL. 13 monomeric compounds are separated and identified, Territrem B shows a remarkable antifungal effect on phytophthora capsici, the EC50 value is 22.96 mu g / mL, mycelial growth, sporangium formation and zoospore generation of the phytophthora capsici can be effectively inhibited, and the defense capacity of pepper plants is enhanced; the form of the phytophthora capsici mycelium is changed, and the integrity of a cell membrane is destroyed. In addition, Territrem B inhibits the activity of antioxidant enzyme, reduces the content of soluble protein and increases the MDA level, thereby inhibiting the normal growth of phytophthora capsici.

The invention discloses bacillus siamensis and application thereof, and relates to the technical field of microorganisms. The strain is bacillus siamensis LYQ15 which is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 14, 2024, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO: 30024. The strain provided by the invention can be used for effectively inhibiting the growth and reproduction of phytophthora and pseudomonas solanacearum as well as related diseases caused by the growth and reproduction of the phytophthora and the pseudomonas solanacearum; in addition, the bacillus siamensis LYQ15 can inhibit reproduction of pathogenic bacteria and epidemic of diseases in an environment with the pathogenic bacteria, and healthy growth of tobacco plants is guaranteed.

This invention relates to the field of agricultural microbiology, and in particular to a strain of *Bacillus erythropoietinus* KF-B6 and its applications, especially in the control of continuous cropping obstacles in apple orchards. The *Bacillus erythropoietinus* KF-B6, with preservation number CGMCC No. 32131, possesses a complex set of functions including rapid and high EPS (explosive peroxide) production, high chitinase production, production of water-soluble and volatile antibacterial substances, and decomposition of macromolecular organic matter. The application of this bacterium, its microbial agents, and compound microbial fertilizers can effectively increase the number of rhizosphere soil bacteria, reduce the number of pathogenic fungi, increase the ratio of bacteria to fungi, and promote the recovery of the soil microbial ecosystem in apple orchards with continuous cropping obstacles; increase the proportion of water-stable aggregates, increase soil organic matter content, and increase soil enzyme activity, thus improving the soil; improve the survival rate of saplings planted during the renewal of new varieties in old orchards with continuous cropping obstacles, promote root vitality and plant growth, and comprehensively alleviate and control continuous cropping obstacles in apple orchards.

This invention provides the application of Evodia rutaecarpa root extract in the preparation of antifungal agents, belonging to the field of agricultural fungal control technology. The active ingredient of the Evodia rutaecarpa root extract is naturally derived and easily obtained; its unique chemical structure and novel mechanism of action effectively avoid the problem of pathogen resistance caused by the concentrated mechanisms of action of existing fungicides, filling the market gap for quinolone-based plant-derived pesticides. The Evodia rutaecarpa root extract exhibits a precise antifungal spectrum and significant inhibitory effect, showing obvious inhibitory activity against soil-borne and interplant-borne plant fungi that are serious threats in agricultural production, such as Phytophthora tobaccoii, Fusarium equisetifolium, Colletotrichum gloeosporioides, and Fusarium moniliforme.

The application discloses a primer composition for detecting Pythium sylvesterianum based on LAMP technology and application thereof, and belongs to the technical field of microorganism detection; the primer composition is a LAMP primer composition designed according to a specific sequence shown in SEQ ID NO. 1. The application adopts a method for rapidly detecting Pythium sylvesterianum, the pathogenic bacterium of stem base rot of raw ginger, based on loop-mediated isothermal amplification technology, and establishes a perfect detection and field real-time detection system for soil samples, water source samples and diseased plant samples, thereby opening up a new train of thought and method for the measurement and prediction of field crop diseases, winning valuable time for the prevention and control of related diseases, and reducing the loss caused by diseases to a certain extent.

This invention discloses the application of reducing the expression of negatively regulated genes in improving resistance to Phytophthora sojae, belonging to the field of plant genetic engineering. Research has found that the soybean genes GmTIFY5a or GmTIFY5b have a negative regulatory function on resistance to Phytophthora sojae. Knocking out GmTIFY5a or GmTIFY5b, or a combination of GmTIFY5a and GmTIFY5b, can enhance soybean resistance to Phytophthora sojae. This can be applied to improving soybean resistance to diseases caused by Phytophthora sojae or to breeding soybean varieties resistant to these diseases. In the soybean cultivar "Williams 82," a double mutant lacking both GmTIFY5a and GmTIFY5b, the accumulation of Phytophthora sojae was reduced by 50-60% compared to the wild type, the lesion infection area was reduced by 80-90%, and the survival rate was increased by 20-30%. Knocking out GmTIFY5a and GmTIFY5b significantly improved plant resistance to Phytophthora sojae. By genetically engineering the above genes to combat plant root rot caused by septicemia, we can not only provide important genetic resources for plant molecular breeding, but also provide an effective means to achieve high plant yields.

The application belongs to the technical field of bio-agriculture, and particularly relates to a gene MOS4 and application of an encoded protein thereof in regulation of plant resistance to parasitic Phytophthora. MOS4 Through gene function research, it is found that overexpression of the gene MOS4 significantly improves the plant resistance to parasitic Phytophthora, and that the expression of the gene MOS4 weakens the plant resistance to parasitic Phytophthora, and that the gene MOS4 positively regulates the plant resistance to parasitic Phytophthora. Further immune mechanism research finds that the gene MOS4 regulates the related genes of a salicylic acid (SA) signal path, interacts with a transcription factor ERF019, and jointly activates the plant immune response. In addition, a dual luciferase experiment proves that the gene MOS4 and the gene ERF019 cooperatively regulate the transcription of PR1. The application provides a new molecular target for prevention and treatment of plant diseases caused by parasitic Phytophthora, and has important agricultural application value.

The invention discloses a pesticide composition containing chloromethylsilazane and application of the pesticide composition in plant disease prevention and growth promotion, and belongs to the technical field of biocide pesticides. According to the invention, the red date kernel extract and the digitaria sanguinalis extract are taken as synergistic extracts and are combined with the chloromethylsilatrane and the 14-hydroxy brassin to prepare the pesticide composition containing the chloromethylsilatrane. When the phytophthora capsici is applied to a test, in a pepper disease resistance test, the disease index is reduced to some extent, and the relative control effect is up to 79.45% at most, which indicates that the phytophthora capsici is inhibited; in the experiment of promoting the growth of the pepper, the plant height of the pepper plant in the compound group is 19.67 + / -0.23 cm, the stem diameter is 4.07 + / -0.05 mm, the dry weight of the overground part is 1.51 + / -0.11 g, the dry weight of the underground part is 0.39 + / -0.04 g, and the growth index is remarkably improved. The pesticide composition containing the chloromethylsilazane prepared by the invention can better inhibit phytophthora capsici, is suitable for developing pesticides for preventing and treating phytophthora capsici, and also has a better promoting effect on the growth of capsicum.

This invention belongs to the field of bio-agricultural technology, specifically relating to genes. DISC1 The application of its encoded proteins in regulating plant resistance to Phytophthora parasiticans. This invention, through the study of... DISC1 Studies on the function of genes and their encoded proteins have revealed that... DISC1 Negative regulation of plant resistance to Phytophthora parasiticum. Overexpressed genes. DISC1 Significantly reduces plant resistance to Phytophthora parasiticum, gene DISC1 The mutant plants exhibited significantly enhanced resistance to Phytophthora parasiticum. Further research into the immune mechanism revealed that the gene... DISC1 This invention regulates plant immune responses by modulating genes related to the jasmonic acid (JA) signaling pathway. It provides a novel gene resource for the control of plant resistance to Phytophthora parasiticum.

The invention provides application of an SlSRC2 gene in regulation and control of tomato broad-spectrum disease resistance, AtSRC2 in arabidopsis thaliana is retrieved through NCBI (National Center of Biotechnology Information), BLAST analysis is performed in a tomato genome by using an amino acid sequence of the AtSRC2, and a protein, namely the SlSRC2, which has the highest similarity with the AtSRC2 in tomatoes is found. Through overexpression of the CDS region of the SlSRC2 in the tomato, the purpose of overexpression of the SlSRC2 is achieved, the alkaloid content in the tomato can be reduced, and the resistance to botrytis cinerea, phytophthora infestans and tomato yellow leaf curl virus is weakened; on the other hand, gene editing is conducted on the specific sequence of the SlSRC2 gene in the tomato by means of the CRISPR-Cas9 technology, the alkaloid content of the obtained mutant plant can be increased, and the resistance of the mutant plant to botrytis cinerea, phytophthora infestans and tomato yellow leaf curl virus is enhanced.

The invention discloses a trichoderma fungus Y4 and application thereof in promoting rice growth, and belongs to the technical field of microorganisms. The classification name of the trichoderma fungus Y4 is Trichoderma sp.Y4, the trichoderma fungus Y4 is preserved in the China Center for Type Culture Collection, and the preservation number of the trichoderma fungus Y4 is CCTCC NO: M 2026215. And biological characteristics and molecular identification are combined to identify that the trichoderma fungus Y4 belongs to a new trichoderma species. When the strain or a fermentation product thereof is applied to a rice culture medium, the growth of rice can be effectively promoted, and the plant biomass is increased. The trichoderma fungus Y4 is developed and prepared into the biological bacterial fertilizer, and a new way is provided for application of microorganisms to development of crop production.

The invention belongs to the technical field of phytopathogen detection, and particularly relates to a specific detection primer and a specific detection kit for Cercospora cf.sigesbeckiae, and application of the specific detection primer and the specific detection kit for the Cercospora cf.sigesbeckiae. The detection primer or the kit provided by the invention can realize specific detection of the siegesbeckia orientalis, and has good intraspecific strain universality. Meanwhile, according to a sensitivity experiment, the lowest detection concentration of the detection system and the detection method established by the invention for the caudobiosporium siegesbeckiae is 0.021 pg.L <-1 >. The detection system can be used for detecting soybean grains with purple spot symptoms in the field, and can be used for quickly and accurately identifying whether soybean plants carry pathogenic bacteria, namely, siegesbeckia orientalis or not. Therefore, the detection primer and the kit provided by the invention have good application prospects.

This invention discloses an endophytic dispersible pantothecin strain X24004 from maize and its applications. The dispersible pantothecin strain of this invention (… Pant disperse X24004, with its preservation number CGMCC No. 34510, exhibits strong antagonistic effects against various pathogenic fungi, including *Fusarium graminearum* (causal agent of corn stem rot), *Fusarium verticillatum* (causal agent of corn ear rot), and *Pythium styracifolium* (causal agent of corn stem rot). It demonstrates high efficiency and broad-spectrum control of plant fungal diseases while also promoting corn plant growth. It is a broad-spectrum biological agent, bio-fertilizer, and seed treatment agent with promising development and application prospects.

The invention belongs to the technical field of biological agriculture, and particularly relates to application of a gene MOS4 and an encoding protein thereof to regulation and control of phytophthora parasitica resistance of plants. The gene function research on the gene MOS4 shows that the resistance of plants to phytophthora parasitica is remarkably improved by overexpressing the gene MOS4, the weakening of the phytophthora parasitica resistance of expression plants of the gene MOS4 is reduced, and the gene MOS4 is found to positively regulate the phytophthora parasitica resistance of the plants. The further study on the immune mechanism finds that the gene MOS4 regulates a salicylic acid (SA) signal channel related gene and interacts with a transcription factor ERF019 to jointly activate the plant immune response. In addition, a dual luciferase experiment proves that MOS4 and ERF019 cooperatively regulate and control transcription of PR1. The invention provides a new molecular target for preventing and treating plant parasitic phytophthora diseases, and has important agricultural application value.

The invention belongs to the technical field of biological agriculture, and particularly relates to application of a gene DISC1 and an encoding protein thereof to regulation and control of phytophthora parasitica resistance of plants. The functional research on the DISC1 and the encoded protein thereof finds that the gene DISC1 negatively regulates the resistance of plants to parasitic phytophthora. The over-expressed gene DISC1 significantly reduces the resistance of the plant to phytophthora parasitica, and the phytophthora parasitica resistance of the gene DISC1 mutant plant is significantly enhanced. The further study on the immune mechanism finds that the gene DISC1 regulates and controls the plant immune response by regulating and controlling the jasmonic acid (JA) signal channel related genes. The invention provides a new gene resource for preventing and treating parasitic phytophthora diseases of plants.

This invention discloses a broad-spectrum Pythium oligandrum strain, Pyo-P2, with broad-spectrum growth-promoting and disease-resistant functions, and its applications. The preservation number of this Pythium oligandrum strain, Pyo-P2, is CGMCC No. 42579. This strain can significantly promote the plant growth and increase biomass of various crops such as potatoes, rice, and soybeans. Simultaneously, it exhibits significant inhibitory activity against multiple pathogens, including Phytophthora soybeanae, Phytophthora pathogenica, Rhizoctonia solani, and Fusarium graminearum. This strain and its microbial preparations possess both growth-promoting and disease-preventing functions, are environmentally friendly, and have broad application prospects in agriculture.

The application provides application of a CaBEH2 gene in enhancing resistance of a pepper to a blight, and belongs to the technical field of plant genetic engineering. The CaBEH2 gene is overexpressed, the cDNA sequence of the CaBEH2 gene is shown as SEQ ID NO:1, the CaBEH2 gene is used as a target gene, overexpression of the CaBEH2 gene is induced by a genetic engineering method, resistance of the pepper to a pepper phytophthora is enhanced, and resistance of the pepper to a blight is enhanced; the blight is a disease caused by the pepper phytophthora. The application provides a pepper blight-related gene CaBEH2 and application thereof, and overexpression of the CaBEH2 gene can enhance the resistance of the pepper plant to the blight, and provides a favorable basis for improving plant disease resistance and cultivating a disease-resistant variety.

The application belongs to the field of molecular biology, and particularly relates to application of OsPLDRP1 protein or its coding gene in improving broad-spectrum disease resistance and regulating agronomic traits of rice. The application constructs OsPLDRP1 knockout mutants and overexpression transgenic lines in a susceptible rice background, and through inoculation of various pathogenic bacteria (including rice blast fungus, sheath blight fungus and bacterial leaf blight fungus), resistance phenotype analysis is carried out, and it is confirmed that OsPLDRP1 is involved in regulating the immune response pathway of rice to fungal diseases (rice blast and sheath blight) and bacterial diseases (bacterial leaf blight). Meanwhile, it is found that the mutant material of OsPLDRP1 is shorter than the wild type in panicle length, and after being driven by a strong promoter ubi, the overexpression material of OsPLDRP1 is looser than the wild type in plant type, the plant is taller, and the early heading phenotype is obtained, and OsPLDRP1 affects the normal growth and development of rice while triggering the immune response of the plant. The application provides important gene resources and molecular theoretical basis for developing new varieties of broad-spectrum disease-resistant rice.

The invention provides application of a CaBEH2 gene in enhancing resistance of pepper to epidemic diseases, and belongs to the technical field of plant genetic engineering. The CaBEH2 gene is over-expressed, and the cDNA (complementary deoxyribonucleic acid) sequence of the CaBEH2 gene is shown as SEQ ID NO: 1; the CaBEH2 gene is used as a target gene, and through a gene engineering method, the excessive expression of the CaBEH2 gene is induced, so that the resistance of the pepper to phytophthora capsici is enhanced, and the resistance of the pepper to epidemic diseases is enhanced; the epidemic diseases are diseases caused by phytophthora capsici. According to the capsicum phytophthora blight related gene CaBEH2 and the application thereof, the resistance of capsicum plants to phytophthora blight can be enhanced through over-expression of the CaBEH2 gene, and a favorable basis is provided for improving the plant disease resistance and cultivating disease-resistant varieties.

The invention discloses a complex microbial inoculant for improving rice productivity and a preparation method and application thereof, and relates to the technical field of agriculture and biology, and the complex microbial inoculant comprises aeromonas intermedius and bacillus pseudomycoides. The intermediate aeromonas is aeromonas Am and is preserved in the China General Microbiological Culture Collection Center on December 17, 2025, the preservation date is December 17, 2025, and the preservation number is CGMCC No.37007. The invention also discloses a preparation method of the intermediate aeromonas. The bacillus pseudofungal is bacillus P and is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, the preservation date is December 17, 2025, and the preservation number is CGMCC No.37008. The bacillus pseudofungal has the advantages that the bacillus pseudofungal is a bacillus strain; the complex microbial inoculant disclosed by the invention has a growth promoting effect on rice plants under saline and alkaline conditions.

The invention discloses application of reduction of expression of a negative regulation gene in improvement of phytophthora resistance of soybean, and belongs to the field of plant genetic engineering. The research finds that the gene GmTIFY5a or GmTIFY5b has the function of negatively regulating and controlling the resistance of the soybean phytophthora root rot. The knockout of GmTIFY5a or GmTIFY5b or the combination of GmTIFY5a and GmTIFY5b can enhance the resistance of soybeans to phytophthora sojae, and can be applied to the improvement of the resistance of soybeans to diseases caused by phytophthora sojae or the cultivation of soybean varieties resistant to the diseases caused by phytophthora sojae. According to the present invention, the accumulation amount of the double mutant soybean phytophthora of the soybean cultivar Williams 82 without GmTIFY5a and GmTIFY5b is reduced by 50-60% compared to the wild type, the disease spot infection area is reduced by 80-90% compared to the wild type infection area, the survival rate is increased by 20-30% compared to the wild type, and the resistance of the plant to the phytophthora can be significantly improved by knocking out the GmTIFY5a and GmTIFY5b. Genetic engineering improvement of plant phytophthora root rot resistance is carried out through the gene, important gene resources are provided for plant molecular breeding, and an effective means is provided for high yield of plants.

The invention relates to the technical field of microorganisms, in particular to cinnamomum migao root endophytic fungi and application thereof. According to the invention, two strains of Trichoderma fungi, namely Trichoderma spp. PA103 and Trichoderma spp. PA423, are separated and identified from cinnamomum migao roots, and the Trichoderma spp. PA103 and the Trichoderma spp. PA423 are used as the Trichoderma spp. PA423. A large number of experiments prove that the cinnamomum migao root endophytic fungus provided by the invention has the effect of remarkably promoting growth and development of cinnamomum migao, and provides technical support for preparing a microbial agent for promoting growth of cinnamomum migao plants.

The application discloses a specific detection target PsRrp8 of soybean phytophthora, a DNA sequence of the specific detection target PsRrp8 is shown as SEQ ID NO. 1, and further provides a primer combination for detecting the soybean phytophthora, the primer combination comprises a forward primer and a reverse primer, a sequence of the forward primer PsRrp8-qPCR-F1 is shown as SEQ ID NO. 2, and a sequence of the reverse primer PsRrp8-qPCR-R1 is shown as SEQ ID NO. 3. P. sojae The minimum DNA concentration of the soybean phytophthora (Phytophthora sojae) detectable by using the detection primer combination provided by the application is 0.332 pg*muL ‑1 , and the quantitative detection technology has high sensitivity. The application can be successfully applied to the detection of the soybean phytophthora in a plant in a phytophthora root rot disease field, can rapidly detect the soybean phytophthora, and provides a new technical means for early warning of diseases.