49 results about "Phytosciara" patented technology

The present invention discloses a gene, GmCDK8, that negatively regulates soybean resistance to Phytophthora spp., and its applications. The disclosed GmCDK8 knockout soybean plants exhibit enhanced resistance to the fungus. Specifically, the results of the soybean cultivar "Williams 82," which lacks GmCDK8, under Phytophthora stress, including root lesion length, leaf lesion area, Phytophthora accumulation, and infected necrotic area on leaves, indicate that knocking out GmCDK8 significantly improves plant resistance to Phytophthora spp. Genetic engineering of this gene for plant resistance to Phytophthora root rot not only provides an important genetic resource for plant molecular breeding but also offers an effective means for achieving high plant yields.

The invention discloses a Chinese chestnut composite phosphate-solubilizing bacterial agent and application thereof, and belongs to the technical field of microorganisms. The invention provides a compound phosphate-solubilizing bacterial agent. The compound phosphate-solubilizing bacterial agent is prepared from phosphate-solubilizing bacteria GXLK-W6 and phosphate-solubilizing fungi GXLK-WZ1-1. The phosphorus-solubilizing bacteria GXLK-WZ1-1 are classified and named as Aspergillus niger, the preservation number of the bacteria is CGMCC (China General Microbiological Culture Collection Center) No.41390, the phosphorus-solubilizing bacteria GXLK-W6 are classified as Burkholderia pyrrolothrix, and the preservation number of the bacteria is CGMCC No.31124. The phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1, the phosphorus-solubilizing bacteria GXLK-WZ1-1 and the phosphorus-solubilizing bacteria GXLK-WZ1-1 and The strains are independently separated from rhizosphere soil of Chinese chestnuts and are screened. And calcium phytate is added into the microbial inoculum to serve as a slow-release phosphorus source. The compound phosphate-solubilizing bacterial agent improves the growth amplification of Chinese chestnut seedlings, promotes the growth of root systems, and promotes the phosphorus absorption of plants. The compound phosphate-solubilizing bacterial agent can improve the dissolution efficiency of inorganic phosphorus in soil and increase the content of soluble available phosphorus, and plays an important role in improving the fertility of the soil.

The invention discloses a gene GmCDK8 for negative regulation and control of phytophthora sojae resistance and application of the gene GmCDK8. GmCDK8-knocked-out soybean plants disclosed by the invention enhance the resistance of soybeans to phytophthora sojae; the results of root scab length, leaf scab area, phytophthora accumulation amount, leaf infected and necrotic areas and the like of the soybean cultivar Williams 82 without the GmCDK8 under the stress of phytophthora show that the resistance of the plant to the phytophthora can be remarkably improved by knocking out the GmCDK8. Genetic engineering improvement of plant phytophthora root rot resistance is carried out through the gene, important gene resources are provided for plant molecular breeding, and an effective means is provided for high yield of plants.

Provided is a stabilized aqueous hydrogen peroxide composition comprising a sugar alcohol that does not contain silver, organophosphonate, peroxyacetic acid or other peracid, or stannate. The composition optionally includes a carboxylic acid. The composition is suitable as a disinfectant and oxidizer, and for the treatment, inhibition, or eradication of fungi or nematodes. Also provided is a method for reversing a bacterial or fungal infection in an infected plant, and a method for enhancing plant growth and / or productivity. Also provided is a method for inhibiting or preventing fungal spore germination in soil in a fungal infested area. Also provide is a method of controlling or preventing nematode infestation of a plant, and a method of reversing damage of a plant caused by nematode infestation. Also provided is a method for treating or reversing Panama disease in an infected banana plant.

The invention discloses a specific detection target PsRrp8 of phytophthora sojae, a DNA (deoxyribonucleic acid) sequence of the specific detection target PsRrp8 is shown as SEQ ID NO.1. The invention further provides a primer combination for detecting the phytophthora sojae, the primer combination comprises a forward primer and a reverse primer, the sequence of the forward primer PsRrp8-qPCR-F1 is shown as SEQ ID NO.2, and the sequence of the reverse primer PsRrp8-qPCR-R1 is shown as SEQ ID NO.3. The invention further provides a kit for detecting the phytophthora sojae. The lowest detectable DNA concentration of the detection primer combination provided by the invention on the phytophthora sojae (P.sojae) is 0.332 pg.L <-1 >, which indicates that the quantitative detection technology has high sensitivity. The method can be successfully applied to phytophthora detection of phytophthora root rot disease field plants, phytophthora sojae can be rapidly detected, and a new technical means is provided for early warning of diseases.

The invention belongs to the technical field of gene engineering, and relates to application of glutaredoxin StGRX5 in plant disease resistance. The invention provides an application of glutaredoxin StGRX5 in the disease resistance of plants. The glutaredoxin StGRX5 is silenced in potato plants, and the resistance of the potato plants to phytophthora infestans can be enhanced.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention discloses a novel application of massinia phosphate solubilizing C22 in prevention and treatment of fungal diseases of plants, and belongs to the technical field of agricultural biology, and particularly relates to a novel application of massinia phosphate solubilizing C22 in prevention and treatment of fungal diseases of plants. The known strain C22 has a remarkable antagonistic effect on pathogenic bacteria fusarium pseudograminearum of wheat stem rot, and the plate inhibition rate reaches 36.79%. Potting and field tests prove that wheat basal stem rot can be effectively prevented and treated by applying the C22 fungicide, the morbidity in the seedling stage is reduced by 21.64%, the morbidity in the adult-plant stage is reduced by 55.06%, the DON toxin content in plants can be remarkably reduced by 68.26%, and the pathogenic bacterium load can be remarkably reduced. According to the invention, the potential of the phosphorus-solubilizing massinia sp. C22 in the aspect of biological control is disclosed for the first time, and a new core microbial resource and a technical scheme are provided for developing a green biological pesticide for controlling wheat stem rot.

The invention discloses a novel leuconostoc screening method and an antibacterial mechanism of leuconostoc on leaf mold of zanthoxylum bungeanum, and relates to the technical field of pathogenic bacteria experiment and analysis. The novel leuconostoc screening method comprises the following steps that S1, materials are prepared, specifically, rotten Chinese prickly ash tree plants are selected, and tested strains in the Chinese prickly ash tree plants comprise plant pathogens of alternaria alternata, fusarium solani, fusarium layered, fusarium oxysporum, fusarium syphilis and colletotrichum pini, and are stored in a refrigerator of a laboratory at the temperature of 4 DEG C for standby application; in the invention, the leuconostoc also has an efficient inhibition effect on field crop pathogenic fungus alternaria alternata, and the research expands the biocontrol spectrum of lactic acid bacteria. Meanwhile, the invention discloses a molecular mechanism that the Leuconostoc suionicum HJ-L synergistically regulates and controls the synthesis of antibacterial substances through lipid metabolism, an ABC transport system and an autophagy pathway, and a theoretical basis and a practical basis are provided for the application of the strain in green prevention and control of the leaf mold of Chinese prickly ash.

The present application belongs to the field of microbial technology, and particularly relates to a fungus in promoting growth of Nauclea officinalis and increasing yield of active substance in a microbial agent. The fungus has a preservation number of GDMCC No: 66543, and belongs to Cladosporium herbarum Cladosporium tenuissimum. Researches show that the fungus can well colonize in the roots of Nauclea officinalis, promote growth of the plant, and increase the yield of vinosamycin lactam in the leaves of Nauclea officinalis, thereby facilitating further improvement of availability of medicinal substance vinosamycin lactam and promoting cultivation of Nauclea officinalis.

The invention belongs to the technical field of plant disease control, and particularly relates to application of amide compounds or agrochemically acceptable salts thereof in preparation of antifungal drugs. The amide compound or the acceptable salt of the amide compound shows remarkable broad-spectrum antibacterial activity on various plant pathogenic fungi such as rhizoctonia solani, tobacco target leaf spot bacteria and magnaporthe oryzae, and has no visible phytotoxicity symptom on plants in an effective concentration range. The action mechanism research shows that the amide compound or the acceptable salt thereof inhibits the growth of fungi through multiple ways: (1) the biological wall and cell membrane structures of the fungi are destroyed to cause the outflow of contents so as to play a bactericidal role; (2) the physiological function of fungal normal cells is interfered, so as to induce programmed death of fungal cells. The invention provides an important reference way for developing a high-efficiency novel agricultural bactericide.

The utility model particularly discloses an insect receiving experiment device for phytophagous insects, which comprises a shell, a shielding device and a plurality of supporting rods, the supporting rods comprise a first gauze element and a second gauze element, and the first gauze element wraps the outer side of the top of a plant and is used for receiving the phytophagous insects; the second gauze element is connected with the outer side of the bottom end of the first gauze element, is arranged in the middle of the plant and is hollow; the shielding device is formed by detachably connecting two halves and arranged on the upper surface of the plant pot body, the upper surface of the shielding device is fixedly connected with the bottom end of the shell, and a through hole allowing a plant stem to penetrate through is formed in the middle of the shielding device; the supporting rods are fixedly connected to the periphery of the shell, and the bottoms of the supporting rods are fixedly connected with the shielding device. According to the utility model, mechanical damage to plants can be reduced.

The invention belongs to the field of marine natural product chemistry, and particularly relates to marine fungi and application thereof in prevention and treatment of pepper phytophthora blight. The marine fungus crude extract disclosed by the invention shows very strong antifungal activity on phytophthora capsici under the concentration of 300 mu g / mL. 13 monomeric compounds are separated and identified, Territrem B shows a remarkable antifungal effect on phytophthora capsici, the EC50 value is 22.96 mu g / mL, mycelial growth, sporangium formation and zoospore generation of the phytophthora capsici can be effectively inhibited, and the defense capacity of pepper plants is enhanced; the form of the phytophthora capsici mycelium is changed, and the integrity of a cell membrane is destroyed. In addition, Territrem B inhibits the activity of antioxidant enzyme, reduces the content of soluble protein and increases the MDA level, thereby inhibiting the normal growth of phytophthora capsici.

The invention discloses bacillus siamensis and application thereof, and relates to the technical field of microorganisms. The strain is bacillus siamensis LYQ15 which is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 14, 2024, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO: 30024. The strain provided by the invention can be used for effectively inhibiting the growth and reproduction of phytophthora and pseudomonas solanacearum as well as related diseases caused by the growth and reproduction of the phytophthora and the pseudomonas solanacearum; in addition, the bacillus siamensis LYQ15 can inhibit reproduction of pathogenic bacteria and epidemic of diseases in an environment with the pathogenic bacteria, and healthy growth of tobacco plants is guaranteed.

This invention relates to the field of agricultural microbiology, and in particular to a strain of *Bacillus erythropoietinus* KF-B6 and its applications, especially in the control of continuous cropping obstacles in apple orchards. The *Bacillus erythropoietinus* KF-B6, with preservation number CGMCC No. 32131, possesses a complex set of functions including rapid and high EPS (explosive peroxide) production, high chitinase production, production of water-soluble and volatile antibacterial substances, and decomposition of macromolecular organic matter. The application of this bacterium, its microbial agents, and compound microbial fertilizers can effectively increase the number of rhizosphere soil bacteria, reduce the number of pathogenic fungi, increase the ratio of bacteria to fungi, and promote the recovery of the soil microbial ecosystem in apple orchards with continuous cropping obstacles; increase the proportion of water-stable aggregates, increase soil organic matter content, and increase soil enzyme activity, thus improving the soil; improve the survival rate of saplings planted during the renewal of new varieties in old orchards with continuous cropping obstacles, promote root vitality and plant growth, and comprehensively alleviate and control continuous cropping obstacles in apple orchards.

The invention discloses an application of PbGAB2 in prevention and control of rape clubroot, a G protein coupled receptor PbGAB2 with high expression level in each stage is screened out from a plasmodiophora brassicae ZJ-1 transcriptome, an RNAi vector of the gene is constructed, a corresponding transgenic plant is obtained, and the application of PbGAB2 in prevention and control of rape clubroot is realized. A disease resistance test of plasmodiophora brassicae shows that the PbGAB2-RNAi transgenic line disclosed by the invention shows excellent disease resistance phenotype, and the G protein coupled receptor PbGAB2 of the plasmodiophora brassicae can be used as a gene resource for resisting clubroot.

This invention provides the application of Evodia rutaecarpa root extract in the preparation of antifungal agents, belonging to the field of agricultural fungal control technology. The active ingredient of the Evodia rutaecarpa root extract is naturally derived and easily obtained; its unique chemical structure and novel mechanism of action effectively avoid the problem of pathogen resistance caused by the concentrated mechanisms of action of existing fungicides, filling the market gap for quinolone-based plant-derived pesticides. The Evodia rutaecarpa root extract exhibits a precise antifungal spectrum and significant inhibitory effect, showing obvious inhibitory activity against soil-borne and interplant-borne plant fungi that are serious threats in agricultural production, such as Phytophthora tobaccoii, Fusarium equisetifolium, Colletotrichum gloeosporioides, and Fusarium moniliforme.

The application discloses a primer composition for detecting Pythium sylvesterianum based on LAMP technology and application thereof, and belongs to the technical field of microorganism detection; the primer composition is a LAMP primer composition designed according to a specific sequence shown in SEQ ID NO. 1. The application adopts a method for rapidly detecting Pythium sylvesterianum, the pathogenic bacterium of stem base rot of raw ginger, based on loop-mediated isothermal amplification technology, and establishes a perfect detection and field real-time detection system for soil samples, water source samples and diseased plant samples, thereby opening up a new train of thought and method for the measurement and prediction of field crop diseases, winning valuable time for the prevention and control of related diseases, and reducing the loss caused by diseases to a certain extent.

This invention discloses the application of reducing the expression of negatively regulated genes in improving resistance to Phytophthora sojae, belonging to the field of plant genetic engineering. Research has found that the soybean genes GmTIFY5a or GmTIFY5b have a negative regulatory function on resistance to Phytophthora sojae. Knocking out GmTIFY5a or GmTIFY5b, or a combination of GmTIFY5a and GmTIFY5b, can enhance soybean resistance to Phytophthora sojae. This can be applied to improving soybean resistance to diseases caused by Phytophthora sojae or to breeding soybean varieties resistant to these diseases. In the soybean cultivar "Williams 82," a double mutant lacking both GmTIFY5a and GmTIFY5b, the accumulation of Phytophthora sojae was reduced by 50-60% compared to the wild type, the lesion infection area was reduced by 80-90%, and the survival rate was increased by 20-30%. Knocking out GmTIFY5a and GmTIFY5b significantly improved plant resistance to Phytophthora sojae. By genetically engineering the above genes to combat plant root rot caused by septicemia, we can not only provide important genetic resources for plant molecular breeding, but also provide an effective means to achieve high plant yields.

The application belongs to the technical field of bio-agriculture, and particularly relates to a gene MOS4 and application of an encoded protein thereof in regulation of plant resistance to parasitic Phytophthora. MOS4 Through gene function research, it is found that overexpression of the gene MOS4 significantly improves the plant resistance to parasitic Phytophthora, and that the expression of the gene MOS4 weakens the plant resistance to parasitic Phytophthora, and that the gene MOS4 positively regulates the plant resistance to parasitic Phytophthora. Further immune mechanism research finds that the gene MOS4 regulates the related genes of a salicylic acid (SA) signal path, interacts with a transcription factor ERF019, and jointly activates the plant immune response. In addition, a dual luciferase experiment proves that the gene MOS4 and the gene ERF019 cooperatively regulate the transcription of PR1. The application provides a new molecular target for prevention and treatment of plant diseases caused by parasitic Phytophthora, and has important agricultural application value.

The invention discloses a pesticide composition containing chloromethylsilazane and application of the pesticide composition in plant disease prevention and growth promotion, and belongs to the technical field of biocide pesticides. According to the invention, the red date kernel extract and the digitaria sanguinalis extract are taken as synergistic extracts and are combined with the chloromethylsilatrane and the 14-hydroxy brassin to prepare the pesticide composition containing the chloromethylsilatrane. When the phytophthora capsici is applied to a test, in a pepper disease resistance test, the disease index is reduced to some extent, and the relative control effect is up to 79.45% at most, which indicates that the phytophthora capsici is inhibited; in the experiment of promoting the growth of the pepper, the plant height of the pepper plant in the compound group is 19.67 + / -0.23 cm, the stem diameter is 4.07 + / -0.05 mm, the dry weight of the overground part is 1.51 + / -0.11 g, the dry weight of the underground part is 0.39 + / -0.04 g, and the growth index is remarkably improved. The pesticide composition containing the chloromethylsilazane prepared by the invention can better inhibit phytophthora capsici, is suitable for developing pesticides for preventing and treating phytophthora capsici, and also has a better promoting effect on the growth of capsicum.

This invention belongs to the field of bio-agricultural technology, specifically relating to genes. DISC1 The application of its encoded proteins in regulating plant resistance to Phytophthora parasiticans. This invention, through the study of... DISC1 Studies on the function of genes and their encoded proteins have revealed that... DISC1 Negative regulation of plant resistance to Phytophthora parasiticum. Overexpressed genes. DISC1 Significantly reduces plant resistance to Phytophthora parasiticum, gene DISC1 The mutant plants exhibited significantly enhanced resistance to Phytophthora parasiticum. Further research into the immune mechanism revealed that the gene... DISC1 This invention regulates plant immune responses by modulating genes related to the jasmonic acid (JA) signaling pathway. It provides a novel gene resource for the control of plant resistance to Phytophthora parasiticum.

The invention provides application of an SlSRC2 gene in regulation and control of tomato broad-spectrum disease resistance, AtSRC2 in arabidopsis thaliana is retrieved through NCBI (National Center of Biotechnology Information), BLAST analysis is performed in a tomato genome by using an amino acid sequence of the AtSRC2, and a protein, namely the SlSRC2, which has the highest similarity with the AtSRC2 in tomatoes is found. Through overexpression of the CDS region of the SlSRC2 in the tomato, the purpose of overexpression of the SlSRC2 is achieved, the alkaloid content in the tomato can be reduced, and the resistance to botrytis cinerea, phytophthora infestans and tomato yellow leaf curl virus is weakened; on the other hand, gene editing is conducted on the specific sequence of the SlSRC2 gene in the tomato by means of the CRISPR-Cas9 technology, the alkaloid content of the obtained mutant plant can be increased, and the resistance of the mutant plant to botrytis cinerea, phytophthora infestans and tomato yellow leaf curl virus is enhanced.

The invention discloses a trichoderma fungus Y4 and application thereof in promoting rice growth, and belongs to the technical field of microorganisms. The classification name of the trichoderma fungus Y4 is Trichoderma sp.Y4, the trichoderma fungus Y4 is preserved in the China Center for Type Culture Collection, and the preservation number of the trichoderma fungus Y4 is CCTCC NO: M 2026215. And biological characteristics and molecular identification are combined to identify that the trichoderma fungus Y4 belongs to a new trichoderma species. When the strain or a fermentation product thereof is applied to a rice culture medium, the growth of rice can be effectively promoted, and the plant biomass is increased. The trichoderma fungus Y4 is developed and prepared into the biological bacterial fertilizer, and a new way is provided for application of microorganisms to development of crop production.

The invention discloses a GmABI5 gene for negative regulation and control of phytophthora sojae resistance and an application of the GmABI5 gene. By constructing a gene silencing expression vector of the GmABI5 gene, results of root scab length, phytophthora accumulation amount and the like of a soybean cultivar 'Williams 82' with low expression of the GmABI5 under the stress of phytophthora by utilizing an agrobacterium tumefaciens-mediated soybean instantaneous transformation system show that the resistance of a plant to the phytophthora can be remarkably improved by silencing the GmABI5. The gene is expected to be used in the following aspects: the disease resistance of phytophthora-sensitive soybeans to phytophthora root rot can be improved by editing the sequence by using a gene editing technology; and resistance gene resource mining and related mechanism research of soybean disease-resistant molecular breeding are facilitated.

The present invention discloses the pepper phytophthora effector NLP779 and its application. The present invention reveals for the first time that NLP779 is a virulence factor of pepper phytophthora, and after spraying it on pepper plants, it can induce an immune response in pepper and inhibit the infection of pepper phytophthora. The stimulation of plant immunity was verified by ROS level detection, callose deposition, MAPKs phosphorylation, etc. Many hormones in plants, such as ethylene, jasmonic acid and salicylic acid, also regulate plant immunity. After exogenous application of NLP779, TMT proteomics analysis was performed, and compared with the control group, a large number of up-regulated proteins were enriched in the ethylene pathway, jasmonic acid pathway, and phenylpropanoid metabolic pathway, indicating that it regulates plant immunity through multiple pathways. The experimental results show that NLP779 has a dual identity as both a virulence factor and an elicitor. The present invention provides an effective means for the prevention and control of pepper phytophthora.

The invention belongs to the technical field of phytopathogen detection, and particularly relates to a specific detection primer and a specific detection kit for Cercospora cf.sigesbeckiae, and application of the specific detection primer and the specific detection kit for the Cercospora cf.sigesbeckiae. The detection primer or the kit provided by the invention can realize specific detection of the siegesbeckia orientalis, and has good intraspecific strain universality. Meanwhile, according to a sensitivity experiment, the lowest detection concentration of the detection system and the detection method established by the invention for the caudobiosporium siegesbeckiae is 0.021 pg.L <-1 >. The detection system can be used for detecting soybean grains with purple spot symptoms in the field, and can be used for quickly and accurately identifying whether soybean plants carry pathogenic bacteria, namely, siegesbeckia orientalis or not. Therefore, the detection primer and the kit provided by the invention have good application prospects.

This invention discloses an endophytic dispersible pantothecin strain X24004 from maize and its applications. The dispersible pantothecin strain of this invention (… Pant disperse X24004, with its preservation number CGMCC No. 34510, exhibits strong antagonistic effects against various pathogenic fungi, including *Fusarium graminearum* (causal agent of corn stem rot), *Fusarium verticillatum* (causal agent of corn ear rot), and *Pythium styracifolium* (causal agent of corn stem rot). It demonstrates high efficiency and broad-spectrum control of plant fungal diseases while also promoting corn plant growth. It is a broad-spectrum biological agent, bio-fertilizer, and seed treatment agent with promising development and application prospects.

The invention belongs to the technical field of biological agriculture, and particularly relates to application of a gene MOS4 and an encoding protein thereof to regulation and control of phytophthora parasitica resistance of plants. The gene function research on the gene MOS4 shows that the resistance of plants to phytophthora parasitica is remarkably improved by overexpressing the gene MOS4, the weakening of the phytophthora parasitica resistance of expression plants of the gene MOS4 is reduced, and the gene MOS4 is found to positively regulate the phytophthora parasitica resistance of the plants. The further study on the immune mechanism finds that the gene MOS4 regulates a salicylic acid (SA) signal channel related gene and interacts with a transcription factor ERF019 to jointly activate the plant immune response. In addition, a dual luciferase experiment proves that MOS4 and ERF019 cooperatively regulate and control transcription of PR1. The invention provides a new molecular target for preventing and treating plant parasitic phytophthora diseases, and has important agricultural application value.