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39 results about "Glutaredoxin" patented technology
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Glutaredoxins are small redox enzymes of approximately one hundred amino-acid residues that use glutathione as a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. In contrast to thioredoxins, which are reduced by thioredoxin reductase, no oxidoreductase exists that specifically reduces glutaredoxins. Instead, glutaredoxins are reduced by the oxidation of glutathione. Oxidized glutathione is then regenerated by glutathione reductase. Together these components compose the glutathione system.
Bacterial thioredoxin reductase inhibitors and methods for use thereof
ActiveUS8592468B2Prevent bacterial growthImprove understandingAntibacterial agentsBiocideBacteroidesEscherichia coli
The mechanism of action of Ebselen differentiates between bacterial and mammalian thioredoxin reductase (TrxR). It displays fast oxidation of mammalian Trx and via the NADPH-TrxR catalyzed turnover of ebselen selenol with hydrogen peroxide, and therefore are mammalian antioxidants. Ebselen, and its diselenide, are strong competitive inhibitors of E. coli TrxR with Ki of 0.14 μM and 0.46 μM, respectively. E. coli mutants lacking glutathione reductase or glutathione were much more sensitive to inhibition by ebselen. Since either glutaredoxin or thioredoxin systems are electron donors to ribonucleotide reductase, ebselen targets primarily glutathione and glutaredoxin-negative bacteria, a class which includes major pathogens. Ebselen, and similar compounds are therefore useful as antibacterial agents, even for multiresistant strains. Two major pathogenic bacteria, which previously had not been known to be sensitive to ebselen, Mycobacterium tuberculosis (tuberculosis) and Helicobacter pylori (stomach ulcer and cancer), were shown to be excellent targets. Helicobacter pylori was also sensitive to ebsulfur.
Owner:THIOREDOXIN SYST AB
Method of skin care and/or treatment using glutaredoxin
InactiveUS20080050332A1Less expensiveCosmetic preparationsHydrocarbon active ingredientsVitamin CLycopene
Methods for the prevention and treatment of skin damage arising from the exposure to UV radiation, air pollution and other stressors capable of leading to the formation of free radicals, using the small-dithiol protein glutaredoxin in a dermatologically acceptable carrier that can be applied topically are disclosed. Some embodiments may include other proteins such as superoxide dismutase and / or catalase, as well as other antioxidant molecules such as reduced glutathione, vitamin E, vitamin C, lycopene, astaxanthin and / or tocotrienols.
Owner:SIVAK HANNAH NAOMI
Bacterial thioredoxin reductase inhibitors and methods for use thereof
ActiveUS20110288130A1Prevent bacterial growthImprove understandingAntibacterial agentsBiocideBacteroidesEscherichia coli
Owner:THIOREDOXIN SYST AB
Bacterial thioredoxin reductase inhibitors and methods for use thereof
InactiveUS20090005422A1Reduced thioredoxinPrevent bacterial growthAntibacterial agentsBiocideBacteroidesEscherichia coli
The mechanism of action of Ebselen differentiates between bacterial and mammalian thioredoxin reductase (TrxR). It displays fast oxidation of mammalian Trx and via the NADPH-TrxR catalyzed turnover of ebselen selenol with hydrogen peroxide, and therefore are mammalian antioxidants. Ebselen, and its diselenide, are strong competitive inhibitors of E. coli TrxR with Ki of 0.14 μM and 0.46 μM, respectively. E. coli mutants lacking glutathione reductase or glutathione were much more sensitive to inhibition by ebselen. Since either glutaredoxin or thioredoxin systems are electron donors to ribonucleotide reductase, ebselen targets primarily glutathione and glutaredoxin-negative bacteria, a class which includes major pathogens. Ebselen, and similar compounds are therefore useful as antibacterial agents, even for multiresistant strains. Two major pathogenic bacteria, which previously had not been known to be sensitive to ebselen, Mycobacterium tuberculosis (tuberculosis) and Helicobacter pylori (stomach ulcer and cancer), were shown to be excellent targets. Helicobacter pylori was also sensitive to ebsulfur.
Owner:THIOREDOXIN SYST AB
Topical use of plant extract for skin detoxification
InactiveUS20090306219A1Maintain cellular redox homeostasisReduce hydrogen peroxideBiocideSulfur/selenium/tellurium active ingredientsPeroxideCollard Green
Specific plant extracts are applied topically to upregulate genes which code for proteins to prevent the build-up of and mitigate the activity of various harmful materials within the skin. These upregulated proteins include glutathione transferases, peroxiredoxins, oxidoreductases, glutaredoxins and glutathione syntheses. These proteins produce additional glutathione, glyoxalase I, maintain cellular redox homeostasis, reduce hydrogen peroxide and alkylhydroperoxides, and conjugate glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Keratinocytes have been induced to significantly upregulate glutathione and glyoxalase I protein expression. Additionally, keratinocytes pre-treated with diallyl trisulfide have increased viability when exposed to ozone, UV radiation or cigarette smoke extract. The active material can be extracted from plants such as garlic, onions, shallots, leeks, chives, scallions, brussel sprouts, broccoli, cabbage, cauliflower, bok choy, kale, mustard, turnip or radish, may include any compound of the allyl sulfide family, and may be of synthetic origin.
Owner:BEILIS DEV
Tomato glutaredoxin gene SlRX1 as well as cloning method and application thereof
InactiveCN101845443AThe method of cloning is simpleThe method of cloning is efficientPlant peptidesFermentationPetalArabidopsis
The invention discloses a tomato glutaredoxin gene SlRX1 as well as a cloning method and application thereof. The tomato glutaredoxin gene SlRX1 is a first glutaredoxin gene separated from tomatoes, consists of 1,003 basic groups and is used for coding CGFS glutaredoxin forced and induced in environments of drought, high salt, and the like. The gene can be expressed in roots, stems, leaves and petals of the tomatoes and participates in an anti-nonbiostress reaction of plants. If the gene is silent in tomatoes, the tomatoes are more sensitive to the environments draught, high salt, oxidativestress, and the like, and if the gene is excessively expressed in arabidopsis thaliana, the resistance to the environments of draught, high salt and oxidativestress of transgenic arabidopsis thaliana. The gene can improve the resistance of plants to the environments of draught, high salt and oxidativestress and has strong theoretical research and application values.
Owner:ZHEJIANG UNIV
Increasing cancer patient survival time by administration of dithio-containing compounds
ActiveUS9023805B2High activityHigh expressionOrganic active ingredientsHeavy metal active ingredientsDiseasePatient survival
Owner:LANTERN PHARMA INC
Plants with enhanced tolerance to multiple abiotic stresses
ActiveUS20170275644A1Improve tolerancePlant peptidesVector-based foreign material introductionBiotechnologyHeterologous
The present disclosure describes genetically-modified plants having enhanced tolerance to multiple abiotic stressors, such as extreme temperatures (heat or cold) and / or drought. Abiotic stress tolerance is enhanced by ectopic expression of a heterologous glutaredoxin. Abiotic stress tolerance (particularly drought) is also enhanced by inhibited function, activity, or expression of an endogenous glutaredoxin. Methods of producing such genetically-modified plants are also disclosed.
Owner:BAYLOR COLLEGE OF MEDICINE +1
Method for evaluating sensitization of natural bee pollen and fermented bee pollen
The invention relates to the technical field of food, and particularly relates to a method for evaluating sensitization of natural bee pollen and fermented bee pollen based on oleosin and glutaredoxin characteristic peptide indexes. According to the invention, the oleosin B2 and the glutaredoxin are used as evaluation indexes for evaluating the sensitization of the untreated natural bee pollen or the fermented bee pollen for the first time, and a specific evaluation process is provided according to the evaluation indexes for evaluating the sensitization of the untreated natural bee pollen or the fermented bee pollen. Meanwhile, on the basis of a specific protein evaluation method, the invention further provides a supplementary means for detecting the contents of oligopeptide and amino acid in the methanol extract of the bee pollen, and further verifies the evaluation result of the sensitization of the bee pollen. The evaluation method provided by the invention has the advantages of high accuracy, convenience and rapidness, and provides an evaluation basis for standardized production and quality control of bee pollen.
Owner:BEE RES INST CHINESE ACAD OF AGRI SCI
Compositions and methods of use of compounds to increase cancer patient survival time
ActiveUS20090232827A1Mitigated and preventedHigh activityBiocideHeavy metal active ingredientsDiseasePatient survival
The present invention discloses and claims compositions, methods of treatment, and kits which cause an increase in time of survival in cancer patients, wherein the cancer either: (i) overexpresses thioredoxin or glutaredoxin and / or (ii) exhibits evidence of thioredoxin- or glutaredoxin-mediated resistance to one or more chemotherapeutic interventions. The present invention also discloses and claims methods and kits for the administration of said compositions to properly treat cancer patients. Additionally, the present invention discloses and claims methods and kits for quantitatively determining the level of expression of thioredoxin or glutaredoxin in the cancer cells of a cancer patient, methods of using those determined levels in the initial diagnosis and / or planning of subsequent treatment methodologies for said cancer patient, as well as ascertaining the potential growth “aggressiveness” of the particular cancer and treatment responsiveness of the particular type of cancer. Further, the present invention discloses and claims novel pharmaceutical compositions, methods, and kits used for the treatment of patients with medical conditions and disease where there is the overexpression of thioredoxin and / or glutaredoxin, and wherein this overexpression is associated with deleterious physiological effects in the patients.
Owner:LANTERN PHARMA INC
Compositions and methods of use of compounds to increase cancer patient survival time
The present invention discloses and claims compositions, methods of treatment, and kits which cause an increase in time of survival in cancer patients, wherein the cancer either: (i) overexpresses thioredoxin or glutaredoxin and / or (ii) exhibits evidence of thioredoxin- or glutaredoxin-mediated resistance to one or more chemotherapeutic interventions. The present invention also discloses and claims methods and kits for the administration of said compositions to properly treat cancer patients. Additionally, the present invention discloses and claims methods and kits for quantitatively determining the level of expression of thioredoxin or glutaredoxin in the cancer cells of a cancer patient, methods of using those determined levels in the initial diagnosis and / or planning of subsequent treatment methodologies for said cancer patient, as well as ascertaining the potential growth 'aggressiveness' of the particular cancer and treatment responsiveness of the particular type of cancer. Further, the present invention discloses and claims novel pharmaceutical compositions, methods, and kits used for the treatment of patients with medical conditions and disease where there is the overexpression of thioredoxin and / or glutaredoxin, and wherein this overexpression is associated with deleterious physiological effects in the patients.
Owner:BIONUMERIK PHARMA INC
Method for synthesizing S-sulfo-L-cysteine through multi-enzyme cascade catalysis
The invention relates to the technical field of biology, in particular to a method for synthesizing S-sulfo-L-cysteine through multi-enzyme cascade catalysis. The invention provides a new route for biosynthesis of S-sulfo-L-cysteine, and a test result shows that serine, AMP, sodium hexametaphosphate and sodium thiosulfate are used as substrates, and the S-sulfo-L-cysteine is synthesized by a one-step method. A crude enzyme liquid which contains serine kinase, polyphosphoric acid-AMP phosphotransferase and phosphoserine dependent type S-sulfo-L-cysteine synthetase in which the background of tryptophanase and glutaredoxin cells is knocked out is used for synthesizing the S-sulfo-L-cysteine, the steps are simple, the product yield is high, the cost is low, and the method has a relatively strong industrial application prospect.
Owner:SHENZHEN READLINE BIOTECH CO LTD
Tomato glutaredoxin gene SlRX1 as well as cloning method and application thereof
InactiveCN101845443BThe method of cloning is simpleThe method of cloning is efficientPlant peptidesFermentationPetalMicrobiology
The invention discloses a tomato glutaredoxin gene SlRX1 as well as a cloning method and application thereof. The tomato glutaredoxin gene SlRX1 is a first glutaredoxin gene separated from tomatoes, consists of 1,003 basic groups and is used for coding CGFS glutaredoxin forced and induced in environments of drought, high salt, and the like. The gene can be expressed in roots, stems, leaves and petals of the tomatoes and participates in an anti-nonbiostress reaction of plants. If the gene is silent in tomatoes, the tomatoes are more sensitive to the environments draught, high salt, oxidativestress, and the like, and if the gene is excessively expressed in arabidopsis thaliana, the resistance to the environments of draught, high salt and oxidativestress of transgenic arabidopsis thaliana. The gene can improve the resistance of plants to the environments of draught, high salt and oxidativestress and has strong theoretical research and application values.
Owner:ZHEJIANG UNIV
Cucumber glutaredoxin gene CsGRX4 and application thereof in susceptibility to botrytis cinerea
The invention relates to the technical field of plant genetic engineering, in particular to a cucumber glutaredoxin gene CsGRX4 and application thereof in susceptibility to botrytis cinerea. The invention discovers an infection of cucumber glutaredoxin gene CsGRX4 by corresponding pathogenic fungus botrytis cinerea, and the susceptibility to the pathogenic fungus botrytis cinerea can be obviously improved by overexpression of the cucumber glutaredoxin gene CsGRX4. After a botrytis cinerea spore suspension is quantitatively inoculated, the disease spot area of a transgenic line of CsGRX4 overexpression is obviously larger than that of a wild type control, and the result shows that overexpression of the cucumber glutaredoxin gene CsGRX4 in arabidopsis thaliana improves the susceptibility of a transgenic plant (the arabidopsis thaliana) to the pathogenic fungus botrytis cinerea.
Owner:NORTHWEST A & F UNIV
Application of glutaredoxin StGRX5 in plant disease resistance
The invention belongs to the technical field of gene engineering, and relates to application of glutaredoxin StGRX5 in plant disease resistance. The invention provides an application of glutaredoxin StGRX5 in the disease resistance of plants. The glutaredoxin StGRX5 is silenced in potato plants, and the resistance of the potato plants to phytophthora infestans can be enhanced.
Owner:NORTHWEST A & F UNIV
Recombinant buckwheat glutaredoxin as well as preparation method and application thereof
ActiveCN106119217AActive purpose regulationPurpose regulates stabilityTransferasesMicroorganism based processesPolygonum fagopyrumEscherichia coli
The invention provides recombinant buckwheat glutaredoxin as well as a preparation method and application thereof. The preparation method comprises the steps of constructing the recombinant buckwheat glutaredoxin (rbGrx) onto a pGEX-6p-1 carrier, transforming into escherichia coli BL21 (DE3), and performing induced expression on fusion protein connected with GST; carrying out enzyme digestion, and then using an anion exchange column and a gel column for further purifying to obtain the rbGrx with the purity of 98% or more. Then, culture is carried out under the crystallization conditions of pH being 4.5, 22% of PEG 3350 (W / V) and 0.1M of CH3COONa, so that a compound crystal of the rbGrx and glutathione (GSH) is obtained; the structure of the compound crystal is measured by an X-ray single-crystal diffraction method, so that important amino acid residues related to catalytic activity can be clearly distinguished, and the stability and enzyme activity are further regulated and controlled purposefully. The rbGrx also has high thioltransferase activity, and the enzyme specific activity is 300U / mg. The rbGrx can be applied to a catalytic thiol transfer reaction, and the crystal and structure of the rbGrx also have a practical value in the aspects of health care, food development, and the like.
Owner:SHANXI UNIV
Grx-roGFP Gpx3-roGFP gene overexpression 16HBE monoclonal cell line model
PendingCN114525307AReveal pathogenic mechanismGenetically modified cellsMicroorganism based processesBacterial virusBronchial epithelium
The invention discloses a construction method of a Grx-roGFP / Gpx3-roGFP gene overexpression 16HBE monoclonal cell line model. The construction method comprises the following steps: step 1, firstly, cloning target genes Grx-roGFP and Gpx-roGFP to a lentiviral vector pLV-puro; and 2, further packaging the lentivirus by using a lentiviral vector pLV-W, infecting 16HBE cells with the lentivirus, screening Grx-roGFP and Gpx-roGFP gene overexpression stable cell strains through puromycin, thereby constructing a cell line capable of dynamically observing the redox dynamic state in bronchial epithelial cell mitochondria in real time, and specifically detecting glutaredoxin (Glutaredoxin, Gpx-roGFP and Gpx-roGFP) of GSH. Grx is fused with glutathione peroxidase Gpx3 and roGFP for specifically detecting H2O2, and glutathione and H2O2 in cells can be dynamically detected in real time by observing the Grx-roGFP and the GPX3-roGFP, so that the change of the oxidation-reduction state in the cells can be speculated, the pathogenesis of active oxygen is disclosed, and a new foundation is laid for research on bacterial and virus infection and tobacco exposure. And lung lesions caused by dust and smoke, weather and air quality in some workplaces are provided with an in-situ model.
Owner:HEBEI MEDICAL UNIVERSITY
Application of glutathione reductase gene in improving aspergillus niger protein secretion
The invention discloses an application of a glutathione reductase gene in improving aspergillus niger protein secretion, and relates to the technical field of gene engineering. According to the invention, by overexpressing a glutathione reductase gene (such as a glutathione reductase gene) or a related gene for providing energy for a glutathione system, ROS in aspergillus niger cells is reduced, and the increase of the total secreted protein amount can be observed through SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis). The obtained recombinant strain can produce amylase with high yield, such as glucamylase, the maximum enzyme activity of the glucamylase can be 3.43 times of wt through shake flask fermentation, the enzyme activity for expressing the glucamylase is enhanced, the expression quantity is remarkably improved, and meanwhile, the expression quantity of other proteins such as acidic amylase and neutral amylase is improved. The recombinant strain can be directly applied to the field of industrial fermentation, and a research thought is provided for wide application of aspergillus niger in the field of industrial enzyme preparations.
Owner:SOUTH CHINA UNIV OF TECH
Application of rubber tree glutaredoxin HbGRX to field of selection of rubber tree tapping panel dryness resistant varieties
InactiveCN109468292AFor effective breedingMicrobiological testing/measurementOxidoreductasesOxygenPlant genetic engineering
The invention belongs to the technical field of plant gene engineering, and particularly relates to rubber tree glutaredoxin HbGRX, and further discloses application of the rubber tree glutaredoxin HbGRX to the field of selection of rubber tree tapping panel dryness resistant varieties. According to the method, the fact that the expression of the rubber tree glutaredoxin HbGRX in a rubber tree with tapping panel dryness shows up an obvious reducing trend is verified through gene cloning performed on the rubber tree glutaredoxin HbGRX and analysis and research on biological information and expression performance, visibly GRX as antioxidant enzyme can effectively eliminate a large amount of accumulated laticiferous cell active oxygen, has close correlation with rubber tree tapping panel dryness, can be effectively applied to the field of prevention and control of rubber tree tapping panel dryness, lays a powerful theoretical foundation for illuminating a rubber tree tapping panel drynessoccurrence mechanism, and further provides a scientific basis for the research and development of a rubber tree tapping panel dryness prevention and control technology and breeding of tapping panel dryness resistant rubber tree new variety through molecular selection.
Owner:RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
A method for evaluating the allergenicity of natural bee pollen and fermented bee pollen
The invention relates to the field of food technology, in particular to a method for evaluating the allergenicity of natural bee pollen and fermented bee pollen based on oleosin and glutaredoxin characteristic peptide indexes. This invention proposes for the first time that oleosin (Oleosin B2) and glutaredoxin (Glutaredoxin) are used as the evaluation index of untreated natural bee pollen or fermented bee pollen allergenicity evaluation, and provides a specific evaluation process accordingly . At the same time, on the basis of the specific protein evaluation method, the present invention further proposes to further verify the evaluation results of the allergenicity of bee pollen by detecting the content of oligopeptides and amino acids in the methanol extract of bee pollen as a supplementary means. The evaluation method of the invention has the advantages of high accuracy, convenience and quickness, and provides evaluation basis for standardized production and quality control of bee pollen.
Owner:BEE RES INST CHINESE ACAD OF AGRI SCI
Construction method of Grx-roGFP/Gpx3-roGFP gene overexpression MDCK monoclonal cell line
PendingCN114277059AReveal pathogenic mechanismMicroorganism based processesFermentationCanine kidneyMetabolite
The invention discloses a construction method of a Grx-roGFP / Gpx3-roGFP gene overexpression MDCK (Madin Darby Canine Kidney) monoclonal cell line. The construction method comprises the following steps: step 1, firstly, cloning target genes Grx-roGFP and Gpx-roGFP to a lentiviral vector pLV-puro; 2, a lentivirus vector pLV-W is further used for packaging into lentivirus to infect MDCK cells, a W gene overexpression stable cell strain is screened through puromycin, and therefore the cell line capable of dynamically observing the redox dynamic state in kidney cell mitochondria in real time is constructed, and an in-situ model is provided for research on reactive oxygen lesion caused by toxicants, drugs and metabolites in the kidney; according to the present invention, GSH and H2O2 capable of accurately reflecting the redox dynamic state in mitochondria are selected, and glutaredoxin (Grx) capable of specifically detecting GSH is fused with glutathione peroxidase Gpx3 and roGFP capable of specifically detecting H2O2; glutathione and H2O2 in cells can be dynamically detected in real time by observing Grx-roGFP and GPX3-roGFP, so that the change of an oxidation-reduction state in a cell body is speculated, and the pathogenesis of active oxygen is disclosed.
Owner:HEBEI MEDICAL UNIVERSITY
Tomato glutaredoxin SIGRXC9 gene and its application
The present invention discloses a tomato glutaredoxin SIGRXC9 gene and its application. The present invention first identifies the tomato glutaredoxin SIGRXC9 gene, knock out of SIGRXC9 in tomato MT (micro-Tom) by Agrobacterium-mediated method for function identification and obtains a result that SIGRXC9 gene deletion results in tomato hypersensitivity to chilling stress, and reduced plant height, leaf length and width, which further demonstrates that SIGRXC9 plays an important role in controlling development and response to low-temperature stress.
Owner:SANYA INST OF HENAN UNIV +1
Neospora attenuated strain with double deletion of grx S16 and grx C5 genes and its construction method and application
ActiveCN111187722BAvoid reinfectionReduce pathogenicityProtozoa antigen ingredientsProtozoaNucleotideTGE VACCINE
The invention discloses a Neospora attenuated strain with double deletion of grx S16 and grx C5 genes, and a construction method and application thereof. This strain is missing the glutaredoxin S16 gene (grx S16) and the glutaredoxin C5 gene (grx C5) of Neospora. The nucleotide sequences of the grx S16 gene and the grx C5 gene are shown in SEQ ID NO.1, respectively. and SEQ ID NO.2. The reproductive capacity and virulence of the Neospora grx S16 and grx C5 double gene deletion strains constructed by the present invention are significantly reduced, and can effectively resist the re-infection of Neospora after inoculating mice, and has the ability to become a Neospora attenuated vaccine. potential.
Owner:CHINA AGRI UNIV
Increasing plant growth and yield by using a glutaredoxin
Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding glutaredoxin proteins, polypeptides encompassing glutaredoxin proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.
Owner:BENSON HILL BIOSYST
Neospora caninum attenuated strain with double deletion of grx S16 and grx C5 genes, and construction method and application thereof
ActiveCN111187722AAvoid reinfectionReduce pathogenicityProtozoa antigen ingredientsProtozoaNeospora speciesNucleotide
The invention discloses a neospora caninum attenuated strain with the double deletion of grx S16 and grx C5 genes, and a construction method and application thereof. The strain lacks the glutaredoxinS16 gene (grx S16) and glutaredoxin C5 gene (grx C5) of neospora caninum, and nucleotide sequences of the grx S16 gene and grx C5 gene are as shown in SEQ ID NO.1 and SEQ ID NO.2, respectively. The strain with the double deletion of grx S16 and grx C5 genes of neospora caninum constructed by the invention is significantly reduced in reproductive capacity and virulence, can effectively resist the reinfection of neospora caninum after inoculation of mice, and has the potential to become a neospora caninum attenuated vaccine.
Owner:CHINA AGRI UNIV
Increasing plant growth and yield by using a glutaredoxin
Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding glutaredoxin proteins, polypeptides encompassing glutaredoxin proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.
Owner:BENSON HILL BIOSYST
Increased stress tolerance, yield, and quality via glutaredoxin overexpression
InactiveUS8519226B2BryophytesOther foreign material introduction processesTemperature stressAgronomy
Oxygenic photosynthesis is the major site of production of reactive oxygen species (ROS). Under high temperature stress, increased ROS damage the photosynthetic machinery, membranes and proteins of plants. The present invention is directed to methods for increasing the stress tolerance of plants by expressing PvGrx5 in the plants.
Owner:UNIV OF FLORIDA RES FOUNDATION INC
Compositions and methods of use of compounds to increase cancer patient survival time
The present invention discloses and claims compositions, methods of treatment, and kits which cause an increase in time of survival in cancer patients, wherein the cancer either: (i) overexpresses thioredoxin or glutaredoxin and / or (ii) exhibits evidence of thioredoxin- or glutaredoxin-mediated resistance to one or more chemotherapeutic interventions. The present invention also discloses and claims methods and kits for the administration of said compositions to properly treat cancer patients. Additionally, the present invention discloses and claims methods and kits for quantitatively determining the level of expression of thioredoxin or glutaredoxin in the cancer cells of a cancer patient, methods of using those determined levels in the initial diagnosis and / or planning of subsequent treatment methodologies for said cancer patient, as well as ascertaining the potential growth 'aggressiveness' of the particular cancer and treatment responsiveness of the particular type of cancer. Further, the present invention discloses and claims novel pharmaceutical compositions, methods, and kits used for the treatment of patients with medical conditions and disease where there is the overexpression of thioredoxin and / or glutaredoxin, and wherein this overexpression is associated with deleterious physiological effects in the patients.
Owner:BIONUMERIK PHARMA INC
Increasing Cancer Patient Survival Time by Administration of Dithio-Containing Compounds
ActiveUS20180369173A9High activityHigh expressionHeavy metal active ingredientsPeptide/protein ingredientsDiseasePatient survival
Owner:LANTERN PHARMA INC
Application of Oryza sativa Grxs (glutaredoxins) gene OsGrxC2 to breeding
ActiveCN107338257AIncreased weight and yieldIncrease productionOxidoreductasesFermentationNucleotideEmbryo
The invention belongs to the technical field of plant genetic engineering and particularly discloses an application of Oryza sativa Grxs (glutaredoxins) gene OsGrxC2 to breeding. The nucleotide sequence of the Oryza sativa Grxs gene OsGrxC2 is shown as SEQ ID NO:1, the overall length of the sequence is 384 bp, and the gene encodes 127 amino acids; the gene OsGrxC2 and a protein encoded by the gene can be used for controlling the abortion degree of plant embryos and have a dosage effect, seeds of homozygous plants genetically modified with the gene OsGrxC2 can have character segregation, embryo-free or embryo-degenerated materials are produced, and the seeds cannot be reserved for planting; meanwhile, 1000-rice-seed weight and rice yield of the homozygous plants genetically modified with the gene OsGrxC2 are increased, and yield and quality of rice are improved; therefore, the gene OsGrxC2 can be used for assisting in breeding novel embryo-free or embryo-degenerated Oryza sativa strains or used for cultivating novel Oryza sativa varieties with high 1000-seed weight and high rice yield and has broad application prospect.
Owner:AGRO BIOLOGICAL GENE RES CENT GUANGDONG ACADEMY OF AGRI SCI