Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cucumber glutaredoxin gene CsGRX4 and application thereof in susceptibility to botrytis cinerea

A technology for disease susceptibility and Botrytis cinerea, which is applied in the field of plant genetic engineering to achieve the effect of improving disease susceptibility

Active Publication Date: 2021-07-16
NORTHWEST A & F UNIV
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] It has been known that glutaredoxin genes in other plants are involved in the regulation of Botrytis cinerea fungal diseases, but the involvement of glutaredoxin genes in cucumber in response to Botrytis cinerea fungal diseases and its function have not been reported.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cucumber glutaredoxin gene CsGRX4 and application thereof in susceptibility to botrytis cinerea
  • Cucumber glutaredoxin gene CsGRX4 and application thereof in susceptibility to botrytis cinerea
  • Cucumber glutaredoxin gene CsGRX4 and application thereof in susceptibility to botrytis cinerea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0019] Cucumber glutaredoxin gene CsGRX4 increases the susceptibility of the pathogenic fungus Botrytis cinerea in transgenic plants (Arabidopsis thaliana).

[0020] According to homologous recombination technology, the present invention refers to the genome sequence of North China type cucumber 9930, uses the first cDNA chain synthesized by reverse transcription of total RNA of cucumber leaves as a template, and amplifies glutaredoxin for the first time through polymerase chain reaction technology Gene CsGRX4. The sequence of the coding region of the gene is 339bp and encodes 133 amino acids. Ana...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of plant genetic engineering, in particular to a cucumber glutaredoxin gene CsGRX4 and application thereof in susceptibility to botrytis cinerea. The invention discovers an infection of cucumber glutaredoxin gene CsGRX4 by corresponding pathogenic fungus botrytis cinerea, and the susceptibility to the pathogenic fungus botrytis cinerea can be obviously improved by overexpression of the cucumber glutaredoxin gene CsGRX4. After a botrytis cinerea spore suspension is quantitatively inoculated, the disease spot area of a transgenic line of CsGRX4 overexpression is obviously larger than that of a wild type control, and the result shows that overexpression of the cucumber glutaredoxin gene CsGRX4 in arabidopsis thaliana improves the susceptibility of a transgenic plant (the arabidopsis thaliana) to the pathogenic fungus botrytis cinerea.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to the application of a cucumber glutaredoxin gene CsGRX4 and its susceptibility to Botrytis cinerea. Background technique [0002] In vegetable production, plant fungal diseases have always been an important factor limiting the improvement of vegetable yield and quality. The response of vegetable crops to fungal infection can be divided into disease resistance or susceptibility, and some key genes or transcription factors play a very important role in pathogen infection and defense response to pathogens. When plants are susceptible to diseases, pathogenic bacteria induce the up-regulation of some key genes or inhibit plant defense responses through infection, including signaling pathways closely related to disease defense responses and the regulation of expression of disease-resistant genes, thereby causing the occurrence of diseases. Therefore, it is very import...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/53C12N15/82A01H5/00A01H6/20
CPCC12N9/0004C12N15/8282C12Y120/04001
Inventor 陈书霞杨玉婷
Owner NORTHWEST A & F UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com