Grx-roGFP Gpx3-roGFP gene overexpression 16HBE monoclonal cell line model
A gene overexpression, 16HBE technology, applied in animal cells, genetic engineering, respiratory/lung cells, etc., can solve the problems of real-time dynamic observation, poor specificity, irreversible cytotoxicity and non-living detection
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[0024] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.
[0025] In the embodiment of the present invention, a method for constructing a 16HBE monoclonal cell line model with Grx-roGFP / Gpx3-roGFP gene overexpression, the construction method steps are as follows:
[0026] Step 1: First clone the target genes Grx-roGFP and Gpx-roGFP into the lentiviral vector pLV-puro;
[0027] The specific steps of the first step are as follows:
[0028] S1: First, select the double-enzyme digested gene fragments Grx-roGFP and Gpx-roGF...
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