Pumpkin mosaic virus infectivity cloning vector and construction method thereof

A technology of pumpkin mosaic virus and cloning vector, which is applied in the field of genetic engineering to achieve the effects of ensuring fidelity, improving infectivity and low transcription cost

A technology of pumpkin mosaic virus and cloning vector, which is applied in the field of genetic engineering to achieve the effects of ensuring fidelity, improving infectivity and low transcription cost

CN109385448AActive Publication Date: 2019-02-26ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Pumpkin mosaic virus infectivity cloning vector and construction method thereof
  • Pumpkin mosaic virus infectivity cloning vector and construction method thereof
  • Pumpkin mosaic virus infectivity cloning vector and construction method thereof

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Embodiment Construction

[0027] The technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings and embodiments. Apparently, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0028] An infectious cloning vector of squash mosaic virus, including the cloning vector of CGMCC No.16296 and the cloning vector of CGMCC No. 16297, classified and named as pumpkin mosaic virus, and was published on September 10, 2018 in Microbiology of China The General Microbiology Center of the Culture Preservation Management Committee conducts the preservation, and the detailed address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0029] A method for constructing an in...

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Abstract

The invention relates to a pumpkin mosaic virus infectivity cloning vector and a construction method thereof. A pumpkin mosaic virus is adopted as a material, genomes 5.8Kb RNA1 (Ribonucleic Acid 1) and 3.3Kb RNA2 are constructed into a plant expression vector pXT1, and then agrobacterium-mediated virus vectors pSqMV-RNA1 and pSqMV-RNA2 with efficient infection capability to host plants are obtained. By adopting the vector, a foundation is laid for studying genomes, gene structures and functions as well as interactions of the virus with hosts, and a novel method is also provided for revealingseed-borne mechanisms of the virus.

Description

technical field [0001] The invention relates to a pumpkin mosaic virus infectious cloning vector and a construction method thereof, belonging to the field of genetic engineering. Background technique [0002] Squash mosaic virus (Squash mosaic virus, SqMV) belongs to Picornaviridae, associated with Cowpeaviridae, Cowpea mosaic virus subfamily, and a member of Cowpea mosaic virus genus. It is an important seed-borne virus on Cucurbitaceae crops. The main host of the virus is melon, watermelon, cucumber, winter squash, pumpkin, zucchini and other Cucurbitaceous crops, but it also infects Chenopodium such as gray vegetables. According to whether different strains of the virus can infect watermelons, and the severity of symptoms induced on melons and pumpkins are different, they can be divided into I and II groups. Among them, the symptoms induced by the strains of group I on melons were more serious than those on pumpkins, and some strains could infect watermelons, while the s...

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Application Information

Patent Timeline
26 Feb 2019
Publication
CN109385448A
IPC
C12N15/82; C12N15/66; C12N15/40; C12R1/94
CPC
C07K14/005; C12N15/66; C12N15/82; C12N2770/00022
Inventors
刘莉铭; 古勤生