114 results about "Phytophthora sojae" patented technology

The invention belongs to the field of gene engineering, and relates to detection target sequence A3apro of phytophthora sojae, and a specific LAMP (loop-mediated isothermal amplification) primer composition and application thereof. The nucleotide sequence of the detection target sequence A3apro of phytophthora sojae is shown as SEQ ID NO.1. The specific LAMP primer composition designed for the A3apro comprises a forward interprimer FIP: SEQ ID NO..2, reverse interprimer BIP: SEQ ID NO.3, forward outerprimer F3:SEQ ID NO.4, and reverse outerprimer B3:SEQ ID NO.5. The specific LAMP primer composition can be applied to detection or identification of the phytophthora sojae. The LAMP primer composition for detecting the phytophthora sojae has the advantages of excellent specificity sensitivity, quick amplification, high efficiency and convenience in identification.

The invention provides a phytophthora sojae molecule detecting primer and a detection reagent kit thereof. The primer comprises a forward primer PSD11 and a backward primer PSD12; and the detection reagent kit comprises PCR buffer solution, MgCl2, dNTPs, Taq polymerase, positive control DNA, DMSO, PSD11/PSD12 and ultrapure water. The detection reagent kit based on the primer specifically amplifies specific amplifying products with the fragment length of 382bp in phytophthora sojae pure DNA, bacteria-bearing pathological tissues and soil. The specific molecule detecting primer and the detection reagent kit thereof can be applied to the rapid, sensitive and specific detection for plant tissues infected with phytophthora sojae and the phytophthora sojae in soil and can be also applied to the early diagnosis of field diseases and the high-sensitive and rapid molecule detection and identification for the phytophthora sojae carried by soybean exporting or importing from customs.

The present invention discloses a detection method of soybean phthoramycin and its special primer. Said special primer is a pair of primers formed from nucleotide sequence of sequence 1 and nucleotide sequence of sequence 2 in sequence table. Said soybean phthoramycin detection method includes the following steps: using genome DNA of material to be detected as template, using the above-mentioned a pair of primers to make PCR amplification and utilizing soybean phthoramycin as positive control, if in the amplification product the identical strip band is existed, said detected material contains soybean phthoramycin.

The invention discloses an actinomycete strain JSX-1 for preventing and curing phytophthora sojae, and belongs to the technical field of plant protection. The strain belongs to streptomycete (Streptomyces sp.) and is named as actinomycete JSX-1. The single colony of the actinomycete strain is circular, white and dry and non-transparent in surface; the hypha is thinner, and grows slowly; the bacterial colony is compact in texture and has a fishy smell. The gram stain of the actinomycete strain is purple, and positive. The JSX-1 fungicide has the controlling effect of 55 to 60 percent on phytophthora sojae. The invention provides the actinomycete strain JSX-1 for preventing and curing phytophthora sojae and the fungicide thereof which are not liable to enable phytophthora sojae to produce resistance, and has no pollution to environment, and the fungicide can be used through root-irrigation after soybean seedling emergence.

This invention relates to the purified and isolated family of Rps1-k disease resistance genes, proteins encoded thereby and use of the same to confer, enhance or otherwise modify resistance of soybean to plant pathogens, particularly Phytophthora sojae.

The invention provides soybean plants having a novel determinant, Rps8, for resistance to Phytophthora sojae. The invention also provides methods for identifying germplasms that are either heterozygous or homozygous for Rps8 using marker assisted selection. Genetic markers with known chromosomal location that are associated with the Rps8 gene are used to confirm Rps8-derived Phytophthora sojae resistance in germplasms. Marker assisted selection also used when introgressing Rps8-derived soybean Phytophthora sojae resistance into non-resistant soybean germplasm or less resistant soybean germplasms.

The present invention belongs to the domain of crop disease prevention and treatment and plant quarantine. Based on soybean phytophthora sojae ITS gene sequence, one pair of specific oligomeric nucleotide primers are designed, and the reagent kit includes Tris. Cl 0.05 mmol, KCl 0.125 mmol, MgCl2 0.005 mmol, dNTPs 0.01 mmol, upstream and downstream primer 0.25 mmol, BSA 0.1 mg, and Taq DNA polymerase 100 U, which are mixed with pure water to prepare 1 ml of the test solution. The soybean phytophthora sojae testing reagent kit based on the primer has powerful specificity, high sensitivity and stability and may be used in fast, sensitive and specific test of soybean phytophthora sojae.

The present invention relates to the field of plant breeding and disease resistance. More specifically, the invention includes a method for breeding soybean plants containing quantitative trail loci (QTL) for resistance the Phytophthora root rot (PRR) caused by Phytophthora sojae. The invention further includes the use of molecular markers in the introgression of PRR resistance QTL into soybean plants.

The invention discloses a biocontrol actinomycete and application. The classification name of the biocontrol actinomycete is streptomyces rochei, and the preservation number is CGMCC NO.17115. The invention further discloses application of the biocontrol actinomycete in prevention and treatment of oomycete diseases. The streptomyces rochei CGMCC 17115 can obviously inhibit the growth of yythium myriotylum and phytophthora sojae, and also has a good inhibition effect on other various pathogens. Fermentation liquor and active extracts of the streptomyces rochei CGMCC 17115 are good in stabilityand resistant to ultraviolet radiation, acid, alkali and heat, and the streptomyces rochei CGMCC 17115 can be prepared into biological preparations and used for biological control over plant diseases.

The invention designs a molecular detection primer special for phytophthora sojae, which adopts R-SNARE protein coding gene Ykt6 needed by cell survival as a target. The primer has high sensitivity and is matched with a universal primer Ykt6F/Ykt6R of Ykt6 phytophthora; a nested PCR method is adopted, so that the sensitivity can be improved by 1000 times and trace phytophthora sojae can be detected; according to the method, specificity and sensitivity are high and hypha, oospore and zoospore of the phytophthora sojae can be detected. Simultaneously, the special for the phytophthora sojae is utilized for developing the detection method for real-time quantitative PCR and a linear regression equation, the amount of infected phytophthora sojae in soybean can be accurately and quantitatively detected and the content of the oospore of the residual phytophthora sojae in the soil can be detected so as to provide forceful data for prediction and forecast of diseases and provide technical support for entry and exit quarantine.

The invention discloses a gene GmWRKY148 capable of improving resistance to phytophthora sojae and application of the gene, wherein the gene can enhance resistance of soybean to the phytophthora sojae. According to the disclosed gene GmWRKY148, the nucleotide sequence of the gene is shown as SEQ ID NO.1. Phenotype and phytophthora accumulation indexes of soybeans Williams with GmWRKY148 overexpression under the phytophthora stress condition indicate that the GmWRKY148 plays an important regulatory role in improving plant resistance to phytophthora. The gene is used for genetic engineering improvement of plant resistance to phytophthora root rot, it is found that the gene has the certain effect on breeding plant varieties with high resistance to phytophthora root rot, and by improving plantresistance to the phytophthora, the plant yield and quality service are improved.

The invention provides a gene GmAP1 capable of improving the disease resistance of plants and application of the gene GmAP1, belongs to the field of plant molecular biology and plant genetic engineering, and relates to a plant source disease-resistant gene GmAP1 and a recombinant expression vector and application thereof. The gene is derived from a soybean, has the nucleotide sequence shown in SEQID NO.1, or has 70% or above of homology with SEQ ID NO.1. The invention further provides a gene silencing and recombinant expression vector. The gene is over-expressed to significantly promote the resistance of the soybean to phytophthora sojae and promote the resistance of tobacco to phytophthora capsici, and is an ideal gene capable of enhancing the disease resistance of the plants. Through agenetic transformation method, the gene is expressed in the soybean or tobacco, so that the gene obtains the resistance ability to various pathogenic bacteria, thereby being capable of improving the disease resistance of crops in the field. The gene GmAP1 can be used in the improvement of the disease resistance of crop breeding.

The invention discloses a method for Phytophthora sojae to inoculate sojae. The method for Phytophthora sojae to inoculate sojae provided by the invention is a method in which the spore suspension of the Phytophthora sojae is used to inoculate a sojae seedling of which the radicel exposes out of the seed capsule. A swarm spore root inoculating method of the invention can do inoculation strictly and quantificationally, and avoids overlarge inoculum concentration to result in that the original disease resistant variety is in susceptibility, thereby ensuring to be more effective and accurate in the application of the filtration of the anti-source; at the same time, the method of the invention has the advantages of small occupied space, less labors, short experiment period and simple latter material treatment in the actual operation, therefore, the method of the invention is suitable for popularization and application.

The invention belongs to the field of biotechnology and discloses application of phytophthora sojae gene PsIR1 capable of inducing plant disease resistance. The invention relates to application of the phytophthora sojae gene PsIR1 (GenBank: HQ231784.1) in constructing a transgenic plant with broad-spectrum resistance against plant pathogen. In the invention, the phytophthora sojae gene PsIR1 is inserted into a PVX expression vector, and the functions of the PsIR1 on tobacco are analyzed through the agrobacterium-mediated instant expression. The result shows that: PsIR1 can obviously improve the resistance of tobacco against phytophthora and can induce the salicylic acid channel to defense the high expression of the response genes PR1 and PR2. Therefore, a conclusion can be made that PsIR1 causes accumulation of salicylic acid, the increase of salicylic acid can improve the broad-spectrum resistance of the plant, and thus the PsIR1 can enhance the disease resistance of tobacco.

The present invention discloses a gene GmAP5 for improving disease resistance of plants and a use of the gene GmAP5, belongs to the field of plant molecular biology and plant genetic engineering, andrelates to the plant-derived disease-resistant gene GmAP5 and a recombinant expression vector and the use of the gene GmAP5. The gene is derived from soybeans and has a nucleotide sequence shown as SEQ ID NO.1. Overexpression of the gene remarkably promotes disease resistance of soybeans to phytophthora sojae and the gene is an ideal gene for enhancing the disease resistance of the plants. The gene is expressed in soybeans or tobaccos through a genetic transformation method, so that the soybeans or tobaccos obtain resistance to various pathogenic microorganisms, and thus field disease resistance of crops can be improved. The gene GmAP5 can be applied to improvement of disease resistance of breeding of the crops.

The invention discloses a seed coating agent for preventing and treating soybean phytophthora root rot, the seed coating agent is composed of a bactericide and a seed initiation and resistance inducer, the bactericide is composed of phenazino-1-carboxylic acid and benthiavalicarb-isopropyl, and the seed initiation and resistance inducer is composed of compound sodium nitrophenolate and brassinolide; the composition comprises the following components in percentage by mass: 2 to 60 percent of the bactericide, 0.005 to 0.05 percent of compound sodium nitrophenolate and 0.0005 to 0.005 percent ofbrassinolide. The formed mixture has good effects of promoting seed germination and strengthening seedlings for soybean seeds; the phenazino-1-carboxylic acid and the benthiavalicarb-isopropyl are lowin toxicity and safe to human, livestock, beneficial organisms and the environment; the drug resistance of germs to a single medicament can be delayed.

The invention relates to plant immunity activator protein PsPII1 and applications thereof, and belongs to the technical field of biological control of crops. The novel plant immunity activator protein, named as PsPII1, is discovered in the culture solutions of phytophthora sojae; and the plant immunity activator protein PsPII1 has strong activity, can induce the resistance and defense response ofplants, can be applied to the disease control of crops, and can be used for the disease control of soybeans, tomatoes, strawberries and tobacco, especially for the prevention of soybean root rot and tobacco mosaic diseases.

The invention provides an AFLP finger-print chromatogram construction of metalaxyl resistance by phytophthora sojae, which belongs to the phythophthora sojae variation detection field. The invention is characterized in that a stable mutant for resistance of metalaxyl by phytophthora sojae is employed, AFLP finger-print chromatogram construction is carried out to obtain specific bands, by constructing the AFLP finger-print chromatogram of metalaxyl resistance by phytophthora sojae, and the invention provides an effective durable resistance species.

The invention provides a method for identifying the resistance of soybeans to soybean phytophthora blight and the application of the method in breeding. The method comprises the steps of (1) inoculant preparation: inoculating a phytophthora sojae strain to a CA culture medium, a V8 culture medium or a lima bean sulture medium, enabling the phytophthora sojae strain to rejuvenate through dark culture, and processing and smashing a culture medium which is rich in thalli to obtain an inoculant; (2) soybean plant material inoculation: containing the inoculant obtained in step (1) in a detection container, selecting a green shanked leaf, which just stretches, of a to-be-detected soybean plant, and inserting a leafstalk in the inoculant in the detection container; (3) dark culture: judging a result after carrying out the dark culture by putting the detection container in which the soybean material is inoculated in step (2) in a humid confined space. The method for identifying the resistance of the soybeans on the soybean phytophthora blight, disclosed by the invention, has the advantages that the operation is simple and convenient, the cost is low, and the time is saved, repeated experiment can be carried out; meanwhile, a to-be-identified experiment material cannot be lost, and the defect in a traditional method that experiment materials are collectively lost can be avoided.

The invention aims to provide a method for preparing a compound extract by the methods of using sasangua cakes as main raw materials, mixing the sasangua cakes with different plant materials accordingto a certain ratio, extracting with ethanol solution after degreasing, and then filtering, concentrating under reduced pressure, and crystallizing and the like. The biological pesticide preparation of compound sasangua cakes for rice blast resistance has a remarkable effect of inhibiting the magnaporthe oryzae, and has a strong inhibition effect on cucumber phytophthora sojae and monilinia fruticola.

The invention discloses a phytophthora root rot resistance gene and application, and belongs to the technical field of soybean genetic breeding. The phytophthora root rot resistance gene is the GmDRR gene of soybeans. The nucleotide sequence of the gene is shown in SEQ ID NO.1. The gene can be used for genetic breeding of plants, especially soybeans. Meanwhile, the invention further provides an application method of the gene, and particularly provides a method for identifying or screening varieties resistant to phytophthora root rot through the gene. By detecting the expression amount of the gene, the resistance to phytophthora root rot can be predicated, and the selection efficiency of phytophthora root rot resistance is greatly improved.

The invention discloses a plant stress resistance-related GmSIZ1a/b protein and its coding gene and application. The protein is a protein selected from the following a) or b): a) a protein composed of an amino acid sequence as shown in the sequence 2/4 in a sequence table; and b) a protein which is obtained after one or more amino acid residues substitution and/or deletion and/or addition of an amino acid sequence as shown in the sequence 2/4 in the sequence table. Experimental results prove that a transgenic line for inhibiting expression of GmSIZ1a/b in soybean obviously raises resistance to phytophthora sojae.

The invention relates to the technical field of molecular biology and gene engineering, in particular to application of a phytophthora sojae oospore development-related transcription factor Myb gene. The phytophthora sojae oospore development-related transcription factor Myb gene has a nucleotide sequence as shown by SEQIDNO.1. A phytophthora sojae Myb transcription factor PsMyb1 gene is the nucleotide sequence as shown by SEQIDNO.1. Protein of the phytophthora sojae Myb transcription factor PsMyb1 gene is a nucleotide sequence as shown by SEQIDNO.2. The PsMyb1 gene provided by the invention mainly aims at regulating and controlling target gene transcription and controlling the development of phytophthora sojae oospores. The gene provided by the invention has a very great value of application to control of phytophthora root rot of soybean. Bactericides which are developed by using the gene as a target have a very important and practical significance to preventing the phytophthora root rot of soybean from occurring and being spread.