39 results about "Phytophthora sojae" patented technology

The invention discloses a specific detection target PsRrp8 of phytophthora sojae, a DNA (deoxyribonucleic acid) sequence of the specific detection target PsRrp8 is shown as SEQ ID NO.1. The invention further provides a primer combination for detecting the phytophthora sojae, the primer combination comprises a forward primer and a reverse primer, the sequence of the forward primer PsRrp8-qPCR-F1 is shown as SEQ ID NO.2, and the sequence of the reverse primer PsRrp8-qPCR-R1 is shown as SEQ ID NO.3. The invention further provides a kit for detecting the phytophthora sojae. The lowest detectable DNA concentration of the detection primer combination provided by the invention on the phytophthora sojae (P.sojae) is 0.332 pg.L <-1 >, which indicates that the quantitative detection technology has high sensitivity. The method can be successfully applied to phytophthora detection of phytophthora root rot disease field plants, phytophthora sojae can be rapidly detected, and a new technical means is provided for early warning of diseases.

The application belongs to the field of plant genetic engineering, and particularly relates to a soybean eugenol synthesis gene GmEGS and application thereof. The application provides application of a soybean eugenol synthesis protein GmEGS or a nucleic acid molecule coding the soybean eugenol synthesis protein GmEGS in increasing the content of eugenol in soybean or improving the ability of soybean to resist Phytophthora sojae, the protein GmEGS includes a protein GmEGS1a, a protein GmEGS1c and a protein GmEGS2a, the amino acid sequences of the protein GmEGS1a, the protein GmEGS1c and the protein GmEGS2a are shown in SEQ ID NO. 1-3 in sequence, and the ORF of the nucleic acid includes nucleic acid molecules shown in SEQ ID NO. 4-6. The content of eugenol in soybean can be obviously increased by transforming a soybean plant with the biomaterial of the application, and meanwhile, the disease resistance of soybean to Phytophthora sojae can also be improved. The application provides a good theoretical basis for the molecular mechanism of disease resistance of soybean, and has important significance for molecular design breeding.

The application belongs to the field of enzyme genetic engineering, and particularly relates to application of a sesquiterpene synthase and a mutant thereof in synthesis of enantiomeric-drimenol compounds, wherein the sesquiterpene synthase is a sesquiterpene synthase from Chenopodium quinoa Willd., and includes CqDMDS1 or CqDMDS2; the mutant of the sesquiterpene synthase is a mutant CqDMDS1 in which an amino acid at position 443 of CqDMDS1 is mutated F443A / G The application realizes heterologous expression of the sesquiterpene synthase and the mutant thereof from Chenopodium quinoa Willd. in an Escherichia coli and a N. benthamiana expression system, catalyzes synthesis of (-)‑8,11-driman-diol or (+)‑drimenol. It is found through activity test that the sesquiterpene synthase from Chenopodium quinoa Willd. has a significant inhibitory effect on plant pathogenic fungi Phytophthora nicotianae, Phytophthora sojae and Fusarium graminearum. The sesquiterpene synthase and the mutant thereof and the biological preparation of the catalytic product have important significance for development of biological pesticides.

The invention relates to the technical field of seed treatment, in particular to a soybean seed coating agent as well as a preparation method and application thereof. Each 1kg of soybean seeds in the coating agent comprise the following components in parts by weight: 0.4-0.6 g of naringenin and 7-12g of auxiliary materials; the auxiliary material comprises one or more of a film-forming agent, an adhesive and a filling agent; the film-forming agent is polyvinyl alcohol, the adhesive is Arabic gum, and the filler is talcum powder. The invention further provides a preparation method of the soybean seed coating agent and application of the soybean seed coating agent in prevention and treatment of soybean root rot. The seed coating agent has the advantages that naringenin is used as a core active component, a uniform protective film is formed on the surface of a coated seed, the seed coating agent has double effects of bacteriostasis and crop protection, mycelial growth of main pathogenic bacteria such as soybean root rot such as phytophthora sojae and fusarium oxysporum can be remarkably inhibited, and the resistance of soybeans to pathogenic bacteria of different physiological races is improved. The coating agent is safe and environment-friendly, simple in preparation process and convenient to use, and meets the large-scale planting requirements of soybeans.

The present application relates to the biological field, and particularly to a soybean Phytophthora psFBP21 protein, a psFBP21 gene and biological materials and application thereof.The amino acid sequence of the soybean Phytophthora psFBP21 protein provided in the present application is shown as SEQ ID NO.2; and the nucleotide sequence of the coding gene is shown as SEQ ID NO.1.Through experiments, it is proved that the soybean Phytophthora psFBP21 protein provided in the present application plays an important role in the growth and development of the soybean Phytophthora itself, and specifically, after the protein is deleted, the mycelium growth of the soybean Phytophthora is slowed down, the number of oospores is reduced, the pathogenicity is reduced, and the like.The above conclusion provides a technical basis for exploring the development and pathogenic molecular mechanism of the soybean Phytophthora, and provides a molecular target for the future research and development of a new fungicide.

This invention discloses an oligosaccharide syltransferase STT3 subunit from *Phytophthora sojae*, its encoding gene, and its applications. The protein sequence of the oligosaccharide syltransferase STT3 subunit provided by this invention is shown in Sequence 2; its encoding gene is shown in Sequence 1. Experiments have demonstrated that the protein provided by this invention plays an important role in the growth and development of *Phytophthora sojae*, specifically manifested in the slowed mycelial growth, reduced number of sporangia and zoospores, and decreased pathogenicity of *Phytophthora sojae* after its deletion. These conclusions provide a technical basis for exploring the molecular mechanisms of *Phytophthora sojae* development and pathogenicity, and provide molecular targets for the future development of novel fungicides.

The invention belongs to the field of plant genetic engineering, and particularly relates to a soybean eugenol synthesis gene GmEGS and application thereof. The invention provides application of a soybean eugenol synthetic protein GmEGS or a nucleic acid molecule encoding the soybean eugenol synthetic protein GmEGS in increasing the content of eugenol in soybeans or improving the resistance of the soybeans to phytophthora sojae. The protein GmEGS comprises a protein GmEGS1a, a protein GmEGS1c and a protein GmEGS2a, and the amino acid sequences of the protein GmEGS1a, the protein GmEGS1c and the protein GmEGS2a are sequentially shown as SEQ ID NO.1-3; the ORF of the nucleic acid comprises nucleic acid molecules as shown in SEQ ID NO. 4-6. When the biological material disclosed by the invention is used for transforming soybean plants, the content of eugenol in soybeans can be obviously increased, and meanwhile, the disease resistance of the soybeans to phytophthora sojae can also be improved. The invention provides a good theoretical basis for a soybean disease-resistant molecular mechanism, and has important significance for molecular design breeding.

This invention discloses the application of reducing the expression of negatively regulated genes in improving resistance to Phytophthora sojae, belonging to the field of plant genetic engineering. Research has found that the soybean genes GmTIFY5a or GmTIFY5b have a negative regulatory function on resistance to Phytophthora sojae. Knocking out GmTIFY5a or GmTIFY5b, or a combination of GmTIFY5a and GmTIFY5b, can enhance soybean resistance to Phytophthora sojae. This can be applied to improving soybean resistance to diseases caused by Phytophthora sojae or to breeding soybean varieties resistant to these diseases. In the soybean cultivar "Williams 82," a double mutant lacking both GmTIFY5a and GmTIFY5b, the accumulation of Phytophthora sojae was reduced by 50-60% compared to the wild type, the lesion infection area was reduced by 80-90%, and the survival rate was increased by 20-30%. Knocking out GmTIFY5a and GmTIFY5b significantly improved plant resistance to Phytophthora sojae. By genetically engineering the above genes to combat plant root rot caused by septicemia, we can not only provide important genetic resources for plant molecular breeding, but also provide an effective means to achieve high plant yields.

The invention discloses a glucosidase protein (GLS1) from phytophthora plant pathogenic oomycetes as well as a coding gene and application thereof. The PsGLS1 protein provided by the invention is a protein of which the sequence is as shown in SEQ ID No. 1 in Phytophthora sojae, and the PsGLS1 protein is a protein of which the sequence is as shown in SEQ ID No. 2 in Phytophthora sojae; the coding gene of the gene is shown as SEQ ID No.2. Experiments prove that the protein provided by the invention plays an important role in the growth and development process of phytophthora sojae, and after the PsGLS1 gene is knocked out, the mycelial growth rate, the sporangium number, the zoospore yield and the resting spore germination rate of the phytophthora sojae are obviously reduced, and the pathogenicity of pathogenic bacteria is obviously influenced. Glucosidase inhibitor mispermine has an inhibition effect on the growth of phytophthora sojae hyphae. In conclusion, the PsGLS1 gene has very high application value in control of epidemic disease occurrence and epidemic caused by phytophthora, and a molecular target is provided for research and development of novel bactericides in the future.

The invention discloses a preparation method of a fusion antibacterial peptide targeting phytophthora sojae, and belongs to the technical field of biology. The invention aims to design a preparation method of a target phytophthora sojae fusion antibacterial peptide by taking phytophthora sojae chitin synthetase as a good bactericide target. The target phytophthora sojae fusion antibacterial peptide is a target phytophthora sojae fusion antibacterial peptide. A pichia pastoris recombinant expression vector of a plurality of antibacterial peptides is constructed, a pichia pastoris expression system is utilized to express the plurality of antibacterial peptides, the pichia pastoris recombinant expression vector is constructed, and the pichia pastoris expression system is utilized to perform high-density fermentation to successfully express the antibacterial peptides. The antibacterial peptide is helpful for enhancing the antibacterial ability of the antibacterial peptide, and has important significance for preventing and treating phytophthora sojae.

The invention discloses a plant immune induced resistance protein Ps131903 and application thereof. The amino acid sequence of the immune induced resistance protein Ps131903 is shown as SEQ ID NO.2. The protein Ps131903 can reduce the harm of phytophthora sojae and pythium ultimum to soybeans, reduce the harm of phytophthora capsici to chilies and tomatoes and reduce the harm of pyricularia grisea to rice. The compound has the advantages of low use concentration, quick effect and broad spectrum, and provides a new way for improving the plant resistance, thereby having wide application prospects in agricultural production.

The application discloses a Gliocladium catenatum PE-1, which is classified as Gliocladium catenatum Clonostachys rosea f. catenulata Clonostachys rosea f , is preserved in the China General Microbiological Culture Collection Center on February 2, 2024, and has a preservation number of CGMCC NO. 41164. The application also discloses a bacterial agent prepared from the Gliocladium catenatum PE-1. The application further discloses applications of the Gliocladium catenatum PE-1 and the bacterial agent in preventing and treating plant diseases. The Gliocladium catenatum PE-1 screened in the application can widely induce the burst of active oxygen of a plant immune marker in monocotyledonous plants and dicotyledonous plants, and can effectively prevent and treat various plant diseases, such as soybean phytophthora root rot, rice rice blast, wheat powdery mildew and wheat scab, and provides a new strain resource and technical support for biological control of plant diseases.

This invention discloses a glucosidase protein (GLS1) from *Phytophthora*, a plant pathogenic oomycete, its encoding gene, and its applications. The PsGLS1 protein provided by this invention is effective against *Phytophthora soybean* (…). Phytophthora sojae The protein in this invention is shown in SEQ ID No. 1; its encoding gene is shown in SEQ ID No. 2. Experiments have demonstrated that the protein provided by this invention plays an important role in the growth and development of *Phytophthora soybeani*. PsGLS1 Gene knockout significantly reduced the mycelial growth rate, sporangium number, zoospore yield, and resting spore germination rate of *Phytophthora soybeani*, and also significantly affected the pathogenicity of the fungus. The glucosidase inhibitor sericite also inhibited the mycelial growth of *Phytophthora soybeani*. In summary, this invention… PsGLS1 Genes have high application value in controlling the occurrence and spread of diseases caused by Phytophthora, and provide molecular targets for the development of new fungicides in the future.

The application discloses a GmSRC2 gene for improving resistance of soybean phytophthora and application, and the soybean plant with the GmSRC2 gene disclosed by the application enhances the resistance of the soybean to the soybean phytophthora, that is, the phenotype, disease spot area, phytophthora accumulation amount and hydrogen peroxide and other physiological indexes of the soybean cultivar "Jack" with overexpressed GmSRC2 under the stress of the phytophthora are detected, and the results show that the GmSRC2 plays an important positive regulation role in improving the resistance of the plant to the phytophthora. Through the gene engineering improvement of the plant resistance to the phytophthora root rot of the transformant, it is found that the gene has a certain effect on cultivating the plant variety with high resistance to the phytophthora root rot, and can improve the resistance of the plant to the phytophthora, thereby serving the improvement of the yield and quality of the plant.

The invention provides a soybean GmACS18 gene and application of a biological material of the soybean GmACS18 gene to improvement of resistance of crops to soybean phytophthora root rot, and belongs to the technical field of genetic engineering. The biological material for overexpressing the gene comprises an expression cassette for overexpressing the GmACS18 gene, a recombinant vector for overexpressing the GmACS18 gene, a recombinant cell for overexpressing the GmACS18 gene, and a plant tissue for overexpressing the GmACS18 gene. It is found that the soybean GmACS18 gene can improve the defense ability of soybeans to phytophthora root rot by improving the ACC synthetase activity and the content of an ethylene synthesis precursor ACC in the ethylene synthesis process. The discovery provides important gene basis and theoretical support for researching related mechanisms of soybean phytophthora root rot resistance, and has important application value in soybean disease-resistant gene engineering breeding.

The invention discloses a strain of pyrethrum delavayi PE-1, the classification name of the pyrethrum delavayi PE-1 is pyrethrum delavayi, the pyrethrum delavayi PE-1 is pyrethrum delavayi, the pyrethrum delavayi is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is February 2, 2024, and the preservation number is CGMCC NO.41164. The pyrethrum delavayi has the advantages that the pyrethrum delavayi can be used for preparing the pyrethrum delavayi; the invention also discloses a microbial inoculum prepared from the strain. The invention further discloses application of the bacillus subtilis and a microbial agent prepared from the bacillus subtilis to prevention and treatment of plant diseases. The fermentation liquor of the screened euphorbia emerald PE-1 can widely induce the burst of reactive oxygen of plant immune markers in monocotyledonous plants and dicotyledonous plants, and can effectively prevent and treat various plant diseases such as phytophthora sojae root rot, rice blast, wheat powdery mildew, wheat scab and the like; new strain resources and technical support are provided for biological control of plant diseases.

Provided are compositions comprising polynucleotides encoding Rps genes, and soybean plants or soybean seeds comprising the compositions and exhibiting resistance to Phytophthora. Additionally, various methods for employing the polynucleotides to increase resistance to Phytophthora are also provided herein.

The invention discloses a chimeric immune receptor and application of the chimeric immune receptor to improvement of phytophthora resistance of plants, and belongs to the technical field of plant disease control. The chimeric immune receptor disclosed by the invention can specifically recognize the phytophthora sojae effector Avh85 or a homolog thereof, and can be activated only when a specific pathogenic bacterium effector exists, so that accurate recognition of target pathogenic bacteria is realized, and unnecessary energy consumption of a plant immune system is avoided. Once the chimeric immune receptor disclosed by the invention is activated, multiple and potent immunoreactions including significant up-regulation of defensive genes, active oxygen outbreak and MAPK phosphorylation can be initiated, a firm defensive barrier is formed together, and expansion of pathogenic bacteria can be rapidly and efficiently suppressed.

The invention belongs to the technical field of plant molecular biology, and discloses a soybean disease resistance-related protease GmRD21A gene, a coding protein thereof, and an application of the gene in plant disease resistance. The nucleotide sequence of the protease GmRD21A gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the polypeptide coded by the protease GmRD21A gene is as shown in SEQ ID NO. 2. Protein spectrum analysis of phytophthora sojae pectate lyase PsPL1 proves that the protease GmRD21A degrades pectate lyase secreted by phytophthora and can resist attack of pathogenic bacteria pectate lyase on plant cell walls, and meanwhile, overexpression of the GmRD21A gene in soybeans can remarkably improve the resistance of plants to phytophthora. The invention can be applied to crop breeding anti-epidemic disease improvement, and is hopeful to improve the disease resistance of plants to epidemic diseases, thereby achieving the purposes of increasing yield and reducing pesticide consumption.

The invention discloses a phytophthora sojae inducible promoter GmJAZ6 and application thereof, and belongs to the technical field of gene engineering. In order to meet the requirements of crop molecular breeding on resistance and growth balance, the invention provides a phytophthora sojae inducible promoter GmJAZ6, and the nucleotide sequence of the inducible promoter GmJAZ6 is as shown in SEQ ID NO. 1; the inducible promoter GmJAZ6 can obviously promote the expression of a reporter gene GUS under the condition that the inducible promoter GmJAZ6 is infected, so that the expression of a disease-resistant gene is driven, the resistance of the inducible promoter GmJAZ6 to the soybean phytophthora root rot is enhanced, and a new way is provided for the disease-resistant breeding of plants.

The invention discloses a preparation method of a pesticide polyurethane nano-microcapsule suspending agent. The pesticide polyurethane nano-microcapsule suspending agent is mainly prepared from the following raw materials according to a mass ratio. The ratio of the pesticide active component to the diphenylmethane diisocyanate to the surface active component to the polyethylene glycol chain segment is (3-8): (1-2): (2-6): 1. Wherein the pesticide active ingredient is pyraclostrobin or fluazinam, and the polyethylene glycol chain segment is PEG200 or PEG400. The preparation method comprises the steps of preparation of an oil phase and a water phase, emulsification and reaction. The dosage form can effectively prevent and treat soybean root rot caused by Phytophthora sojae and / or Fusarium oxysporum, in addition, the composition formed by compounding the two nano-microcapsule suspending agents of pyraclostrobin and fluazinam according to the mass ratio of 1: 1-4: 1 shows a synergistic effect, is suitable for seed treatment, shows an excellent prevention and treatment effect, and can be used for preventing and treating the soybean root rot caused by the pyraclostrobin and the fluazinam. A new way is provided for preventing and controlling plant diseases.

This invention discloses a broad-spectrum Pythium oligandrum strain, Pyo-P2, with broad-spectrum growth-promoting and disease-resistant functions, and its applications. The preservation number of this Pythium oligandrum strain, Pyo-P2, is CGMCC No. 42579. This strain can significantly promote the plant growth and increase biomass of various crops such as potatoes, rice, and soybeans. Simultaneously, it exhibits significant inhibitory activity against multiple pathogens, including Phytophthora soybeanae, Phytophthora pathogenica, Rhizoctonia solani, and Fusarium graminearum. This strain and its microbial preparations possess both growth-promoting and disease-preventing functions, are environmentally friendly, and have broad application prospects in agriculture.

The application relates to the field of agricultural biotechnology, and particularly discloses a KASP molecular marker for identifying the resistance of soybeans to phytophthora root rot and application. The KASP molecular marker primer sequence is shown in the upstream primer SEQ ID No. 1 (connected with a FAM fluorescent tag), the upstream primer SEQ ID No. 2 (connected with a VIC fluorescent tag) and the universal downstream primer SEQ ID No. 3. The KASP molecular marker can realize efficient, accurate and low-cost identification and genotyping of the resistance of soybeans to phytophthora root rot, and the effective identification and detection reaches 98.55%, and the accuracy reaches 84.80%.

The invention belongs to the technical field of plant molecular breeding, and discloses an InDel molecular marker related to soybean phytophthora root rot resistance and application. The InDel molecular marker is designed on the basis of 7bp deletion variation of a soybean GmRALF18 gene (Glyma. 19G209900), and a detection primer is developed on the basis of the 7bp deletion variation of the soybean GmRALF18 gene (Glyma. 19G209900). A soybean wild type, a GmRALF18 gene heterozygous mutant and a homozygous mutant can be quickly distinguished by combining conventional PCR (Polymerase Chain Reaction) amplification with polyacrylamide gel electrophoresis: the wild type only has a band of about 122bp (susceptible soybean phytophthora root rot), the homozygous mutant only has a band of about 115bp (highly resistant soybean phytophthora root rot), and the heterozygous mutant simultaneously has two bands. The InDel molecular marker disclosed by the invention is high in specificity, low in detection cost and simple and convenient to operate, does not need a fluorescent modification or enzyme digestion step, can realize high-throughput screening of high-phytophthora root rot resistant materials in soybean breeding offspring, and has important industrial application value.

The invention discloses a small-molecule inhibitor of a targeted phytophthora sterol sensing receptor SSRK1 and application of the small-molecule inhibitor, and belongs to the technical field of pesticide biology. The inhibitor can specifically bind to a sterol binding site of SSRK1 and competitively block a natural sterol signal, so that comprehensive inhibition on growth, development and pathogenicity of phytophthora is realized. The compound shows remarkable bacteriostatic activity on various phytophthora (such as phytophthora sojae, phytophthora infestans and phytophthora capsici), and can effectively prevent and treat crop diseases such as soybean root rot, potato late blight and phytophthora capsici. The small-molecule inhibitor provided by the invention has the characteristics of novel target spot, unique action mechanism, broad spectrum, high efficiency, environmental friendliness and the like, is not easy to generate cross resistance with the traditional bactericide, and provides a new candidate molecule and preparation development approach for green prevention and control of phytophthora.

The present invention relates to a method for controlling or preventing an infestation of useful plants by a phytopathogenic microorganism selected from the group consisting of Fusarium pseudograminearum, Fusarium culmorum, Fusarium oxysporum, Fusarium graminearum, Bakania oryzae, Sporidesmium humicola, Botrytis cinerea, Phytophthora sojae, Phytophthora capsici, Phytophthora infestans, Pythium aphanidermatum, Pythium arrhenomanes, Pythium deliense, Puccinia striiformis, Puccinia hordei, Puccinia malvaccarum, Pseudoperonospora cubensis and Sphaerotheca fuliginea, which comprises the application of a compound of formula (I) to useful plants, to the locus thereof, or to their propagation material.

The present invention relates to the use of bacteria for inhibition of plant disease. In particular, the present invention relates to the use of a Bacillus amyloliquefaciens strain and variants thereof, capable of inhibiting growth of phytopathogens, such as fungi and oomycetes including Fusarium culmorum, Fusarium oxysporum, Pythium irregulare, Pythium selbyi and Phytophthora sojae.

The invention relates to the technical field of biomedical engineering, and discloses a method for promoting root rot resistance by analyzing soybean core endophyte flora based on fluorescence labeling, and the method comprises the following steps: S1, respectively separating microbial communities from root systems of healthy soybean plants and susceptible soybean plants to obtain a healthy group microbial suspension and a susceptible group microbial suspension; according to the method for promoting root rot resistance by analyzing soybean core endophyte flora based on fluorescence labeling, phytophthora sojae and associated microorganisms are specifically recognized through an immunofluorescence labeling technology, fluorescence-negative flora in healthy soybean root systems is precisely enriched in combination with flow cytometry sorting, screening leakage of non-culturable microorganisms is avoided from the source, and the yield of root rot resistance is improved. Meanwhile, the invalid workload in the screening process is greatly reduced, the mining period of the core beneficial endophytic flora is shortened, and the pertinence and efficiency of the screening process are improved.

This invention discloses an arginine methyltransferase protein from *Phytophthora* plant pathogenic oomycetes, its encoding gene, and its applications. The arginine methyltransferase protein provided by this invention is shown in sequences 6-8; its encoding gene is shown in sequences 1-3. Experiments have demonstrated that the protein of this invention plays an important role in the growth, development, and pathogenicity of *Phytophthora capsici*, a typical plant pathogenic oomycete. The absence or reduced expression level of this protein leads to slower mycelial growth rate, reduced sporangium production, decreased zoospore numbers, and reduced pathogenicity of *Phytophthora capsici*. Corresponding arginine methyltransferase inhibitors exhibit varying degrees of activity against typical plant pathogenic oomycetes such as *Phytophthora capsici*, *Phytophthora soybeani*, and *Phytophthora pathogenica*. The gene provided by this invention has significant application potential in the prevention and control of crop blight caused by *Phytophthora* plant pathogenic oomycetes. Novel fungicides developed based on this protein as a target have important practical significance for controlling the occurrence and spread of crop blight caused by *Phytophthora*.