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Method for vaccination soybean with phytophthora sojae

A technology for Phytophthora sojae and soybeans, which is applied in horticultural methods, botanical equipment and methods, plant genetic improvement and other directions, can solve the problems of high risk, large space occupation, long time required, etc. The effect of small footprint and short experimental period

Inactive Publication Date: 2008-09-24
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the hypocotyl wound inoculation method is relatively mature as an international general inoculation method, it also has its disadvantages: the method is difficult to control the size of the bacterial block, the amount of the bacteria and the age of the bacteria, which is generally lower than the inoculation amount of the actual field disease. The amount of inoculation is too high, and generally medium or low resistance varieties may be identified as susceptible; in addition, the disease resistance identified by this method is mainly resistance to expansion, and the identification of varieties resistant to invasion is not accurate , thus reducing the number of resistant sources screened, especially for the screening of horizontally resistant varieties is very unfavorable, and this method is easy to cause plant death due to too large incision during operation, which will lead to wrong screening; For inoculation, at least 10 plants are required for each treatment, and 30 plants are repeated 3 times. It requires potting conditions, takes up a lot of space, takes a long time, and has a large workload; Phytophthora soybean root rot is a quarantine disease, and the amount of experimental materials Large, labor-intensive and time-consuming to handle, high risk

Method used

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  • Method for vaccination soybean with phytophthora sojae
  • Method for vaccination soybean with phytophthora sojae
  • Method for vaccination soybean with phytophthora sojae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1, the determination of experimental procedure of zoospore root inoculation method and inoculation amount

[0015] In this example, the No. 1 physiological race of Phytophthora sojae (Xu Xiuhong, Lu Huiying, Qu Juanjuan, Yang Qingkai. Identification and toxicity analysis of physiological race of Phytophthora sojae root rot, Journal of Plant Protection, 2003, 30(2): 125 -128) is the experimental strain, and Suinong 10 (disease resistant variety) and sloan (susceptible variety) are used as experimental soybean varieties.

[0016] The specific operations are as follows:

[0017] (1) Preparation of soybean seedlings

[0018] Moisturize the sloan soybean seeds for 2-3 days until the radicle length is about 2cm, select the seedlings with the same radicle length, and divide them into ampoules (volume 10ml) containing the same amount of sterilized tap water. The shaft 1cm and the roots are all immersed in sterile water, and the cotyledons are left outside the bottl...

Embodiment 2

[0033] Example 2. Application of the zoospore root inoculation method in the screening of antibiotics

[0034] The bacterial strains used in this example are as shown in Table 2 (Li Ying, Yang Mingxiu, Wen Jingzhi, Phytophthora soya bean 1st generation single zoospore strain population variation, Chinese Journal of Oil Crops, 2007(4): 460- 465). The soybean varieties used in this example are 9 internationally used Phytophthora sojae identification hosts and 11 commonly used varieties in production. The variety catalog is shown in Table 3.

[0035] Table 2. Phytophthora sojae physiological race and its corresponding toxicity formula (Li Ying, 2007)

[0036] serial number

Toxicity formula

physiological race

serial number

Toxicity formula

physiological race

1

2

3

1a, 7

1a

3a, 1b, 7

3

5

6

7

3a, 7

7

1b, 7

15

1

2

4

1a, 1c, 7

...

Embodiment 3

[0057] Example 3. Screening of Antibiotics with Zoospore Root Inoculation Method

[0058] The 32 soybean varieties used in this example are commonly used varieties in production, and the other physiological races except No. 1 physiological race in Table 2 are used for testing respectively.

[0059] Infect the soybean seedlings of 32 varieties shown in Table 4 with the other physiological races except No. 1 physiological race in Table 2, wherein, the experimental method of each strain infecting the soybean seedlings of each variety, except the inoculation concentration Except for 250 spores / 1 soybean seedling, the other methods are the same as those described in Example 1.

[0060] The experiment was repeated three times, and the results are shown in Table 4.

[0061] Table 4. Identification results of 32 soybean variety resources against Phytophthora root rot

[0062]

[0063]

[0064] Note: S is susceptible; R is resistant

[0065] The experimental results showed tha...

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Abstract

The invention discloses a method for Phytophthora sojae to inoculate sojae. The method for Phytophthora sojae to inoculate sojae provided by the invention is a method in which the spore suspension of the Phytophthora sojae is used to inoculate a sojae seedling of which the radicel exposes out of the seed capsule. A swarm spore root inoculating method of the invention can do inoculation strictly and quantificationally, and avoids overlarge inoculum concentration to result in that the original disease resistant variety is in susceptibility, thereby ensuring to be more effective and accurate in the application of the filtration of the anti-source; at the same time, the method of the invention has the advantages of small occupied space, less labors, short experiment period and simple latter material treatment in the actual operation, therefore, the method of the invention is suitable for popularization and application.

Description

technical field [0001] The invention relates to a method for inoculating soybean with Phytophthora sojae, in particular to a zoospore root inoculation method. Background technique [0002] Phytophthora root rot is caused by the infection of Phytophthora sojae Kaufmann & Gerdemann. It is one of the most serious and devastating soybean diseases, and the environmental conditions are conducive to the occurrence of the disease. can lead to the failure of soybean production. Soybean disease-resistant breeding is the most direct and effective way to control Phytophthora soja root rot. Since the mid-1990s, large-scale screening of germplasm resources against Phytophthora sojae root rot has been carried out in China. The basic method of anti-source screening is to use the hypocotyl wound inoculation method proposed by Kaufmann and Gerdemann. The hypocotyl wound inoculation method, as an international general inoculation method, is relatively mature, but it also has its shortcoming...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06A01H1/06A01H1/04
Inventor 文景芝李永刚杨明秀
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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