Bacillus velezensis 19573-3 and application thereof
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A bacillus and colony technology, applied in the field of bioengineering, can solve problems such as drug resistance of plant pathogens, and achieve the effect of superior research potential and application value
Active Publication Date: 2021-08-06
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology
In the past few decades, the prevention and control of plant diseases has relied too much on chemical means. However, the long-term and irrational application of chemical fungicides has caused many negative effects on the soil, water and other environments, and also directly led to the resistance of plant pathogens to a certain extent. production
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Embodiment 1
[0031] Example 1: Screening of Bacillus Velez 19573-3:
[0032] The rhizosphere soil of peanuts in Mazhuang Village, Mazhuang Town, Daiyue District, Tai'an, Shandong Province was collected, and the soil samples were mixed using the 5-point sampling method, packed into sterile kraft paper bags, sealed and transported to the laboratory. Weigh 10g of soil in a triangular flask filled with 90ml of sterile water, vibrate on a shaker at 150 r / min for 30min, and prepare 10 -1 ~10 -6 series of soil suspensions, take 10 of them -1 , 10 -2 The suspension was maintained in a water bath at 80°C for 10 minutes to kill non-bacillus bacteria, while 10 -3 、10 -4 、10 -5 、10 -6The suspension was not heat-treated. Draw 0.1ml of a series of soil suspensions, respectively add beef extract peptone and wort beef extract peptone agar medium plates, spread evenly, repeat each dilution 3 times, and incubate at 28°C for 3 to 4 days. According to the morphological characteristics of the bacteri...
Embodiment 2
[0033] Embodiment 2: the application of Bacillus Velez 19573-3:
[0034] 1. Materials and methods:
[0035] 1. Test strains:
[0036] Bacillus Veles 19573-3, 19-5036, Bacillus amyloliquefaciens 19544-1, 19544-R, 19698-2, Bacillus licheniformis isolated and preserved in the Laboratory of Bacterial Fertilizer Testing Center, Institute of Agricultural Resources and Agricultural Regional Planning, Chinese Academy of Agricultural Sciences Bacillus 19423-1, 19593-2, 19490-1, Bacillus subtilis 19479, 19482, 19485-1, 19487, 19543-1, 19590, 19605, 19644, 19699, 2, 12, Bacillus spinosa 1, 3 , 4, 5, 6, 7, 7R, 8, 11, 13 and other 29 plant rhizosphere growth-promoting bacterial strains, which were activated and cultured at 28°C with LB medium before use.
[0037] Pathogens: Fusarium oxysporum BNCC 120618 and Botrytis cinerea BNCC 338228 were purchased from Beina Chuanglian Biotechnology Co., Ltd. Wheat root rot, Phytophthora soybean, Pythium ultima and Botrytis cinerea were preserved in ...
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Abstract
The invention discloses bacillus velezensis 19573-3 and application thereof, and belongs to the technical field of biological engineering. The bacillus velezensis 19573-3 has the preservation number of CGMCC No. 21866. The yield of Fengycin in fermentation liquor of the strain is 90.31 [mu]g / ml when the retention time is 4.676 minutes and 45.07 [mu]g / ml when the retention time is 4.893 minutes respectively; the strain has the capacity of producing cellulase, protease and siderophore; and SA, 2iP, IAA and ACC deaminase are generated in the fermentation liquor of the strain. The invention also relates to the application of the strain in inhibiting the activity of fusarium oxysporum BNCC120618, botrytis cinerea BNCC338228, wheat root rot, phytophthora sojae, pythium ultimum and botrytis cinerea. The bacillus velezensis 19573-3 has the advantages that the bacillus velezensis 19573-3 has the potential of synthesizing and secreting various antibiotics and antibacterial peptides, so that the bacillus velezensis 19573-3 has wide and diversified functions and effects in plant disease prevention and growth promotion.
Description
technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a Bacillus Velez 19573-3 and an application thereof. Background technique [0002] The plant diseases caused by the infection of plant pathogens will cause huge economic losses to the grain production of the whole country and even the whole world every year, and it is one of the main factors restricting the high and stable grain yield and food safety. In the past few decades, the prevention and control of plant diseases has relied too much on chemical means. However, the long-term irrational application of chemical fungicides has caused many negative effects on the soil, water and other environments, and also directly led to the resistance of plant pathogens to a certain extent. generation. Therefore, it is imminent to explore new methods of environment-friendly plant disease control. Biological control is environmentally friendly, safe and efficient, and mee...
Claims
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