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Biological pesticide preparation of compound sasangua cake for rice blast resistance

A technology for biological pesticides and rice blast, applied in the directions of biocides, chemicals for biological control, biocides, etc., can solve the problems of low activity, short duration of drug efficacy, high production costs, and achieve low process costs. , the effect of easy industrial production and low price

Inactive Publication Date: 2018-12-21
SUZHOU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, botanical pesticides often have disadvantages such as low activity, short duration of efficacy, and high production costs.

Method used

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  • Biological pesticide preparation of compound sasangua cake for rice blast resistance
  • Biological pesticide preparation of compound sasangua cake for rice blast resistance
  • Biological pesticide preparation of compound sasangua cake for rice blast resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Plant materials such as tea leaves, neem seeds, vitex, licorice, psoralen and other plant materials are baked in an oven at 70°C to constant weight, then weigh 30-100g of tea leaves, 20-30g of tea leaves, and 20-30g of neem seeds , 7-10g of Vitex fresh leaves, 5-10g of licorice, and 7-10g of psoralen fruit were mixed and crushed, passed through a 40-mesh sieve, degreased, and then added 1500-2000mL 70% ethanol for 3 times, each time 8 -12 hours, respectively, through ethanol reflux extraction, filtration, concentration under reduced pressure, freeze-drying and other operations to obtain crude dry powder, which is made into powder after crushing, stirring and mixing. The ratio of medicine to water is 1:200-500.

Embodiment 2

[0026] Get the main active ingredient of the compound tea dead biological pesticide obtained in Example 1, and dilute it into 5mg / mL, 6.6mg / mL, 10mg / mL, 13.3mg / mL, 15mg / mL, 20mg / mL according to the extraction mass gradient, and use mycelia Growth rate method was used to determine the inhibitory rate of the extract on the growth of rice blast mycelia. Add 1 mL of each of the preparations with 6 concentration gradients to the sterilized petri dish, add the same amount of sterile water to the control group, and pour 9 mL of PSA medium at a suitable temperature (the hand is not hot after long-term contact) into each petri dish , and immediately shake gently until solidified. Use a puncher to punch out bacterial disks (diameter 9mm) from the edge of the vigorously growing Pyricularia oryzae colony after 5 days of cultivation, pick one piece each and move it into the corresponding plate, turn the side with mycelium upside down and place it on the surface of the medium , press sligh...

Embodiment 3

[0030] Get the compound tea withered biological pesticide main active ingredient of embodiment 1 gained, be diluted to 10.00, 5.00, 3.33, 2.50, 2.00mg / ml totally 5 concentration gradients by extraction mass gradient, adopt hanging drop method to measure compound tea withered biological pesticide to Inhibitory effect of spores of Phytophthora oryzae. Cultivate Magnaporthe grisea for a certain period of time, and after producing spores, prepare spore suspensions of compound tea dead biological pesticides with different concentrations, and use sterile water as the control (CK), repeat 3 times. Microscopically observe the germinated spore morphology within 12 to 36 hours ( figure 2 ).

[0031] Depend on figure 2 It can be seen that different concentrations of the compound tea dead biological pesticide have inhibitory effect on the conidia germination of the rice blast fungus. It can be seen from the control group that the conidia of the rice blast fungus have normal morphology...

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Abstract

The invention aims to provide a method for preparing a compound extract by the methods of using sasangua cakes as main raw materials, mixing the sasangua cakes with different plant materials accordingto a certain ratio, extracting with ethanol solution after degreasing, and then filtering, concentrating under reduced pressure, and crystallizing and the like. The biological pesticide preparation of compound sasangua cakes for rice blast resistance has a remarkable effect of inhibiting the magnaporthe oryzae, and has a strong inhibition effect on cucumber phytophthora sojae and monilinia fruticola.

Description

technical field [0001] The invention belongs to the field of biopesticides, and relates to a biopesticide preparation and application of a compound tea-cured rice blast. Background technique [0002] Rice is one of the most important food sources in the world. The most common disease of rice is rice blast, which is a fungal disease belonging to rice diseases. The main harm of rice blast is to cause rice yield reduction, usually resulting in a loss of 10% to 20% of rice yield, some losses up to half, or even no harvest in some areas. Since the 1990s, rice blast has frequently broken out in my country, with an average annual outbreak area of ​​about 3.8 million hectares and a loss of millions of tons of rice. [0003] For a long time, the means to prevent and control rice blast mainly include the use of chemical pesticides, the cultivation of physiological races resistant to rice blast, and the strengthening of field management. Among them, chemical pesticides have become t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/26A01N25/14A01P3/00
CPCA01N25/14A01N65/00A01N65/08A01N65/20A01N65/26
Inventor 陈宏伟陈佳佳刘佳王志伟周颖吕勇林李良智
Owner SUZHOU UNIV OF SCI & TECH
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