Compound biopesticide preparation for controlling tea tree anthracnose
A technology for biological pesticides and anthracnose, which is applied in the directions of biocides, chemicals for biological control, biocides, etc., can solve the problem of lack of relatively safe and effective research reports on chemical control, and achieve the convenience of comprehensive utilization of resources, Easy to degrade and the effect of producing a wide range of raw materials
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Embodiment 1
[0036]Camellia oleifera meal, tea leaves, big thistle, clematis, wild chrysanthemum and other plant materials are baked in an oven at 70°C to constant weight, then weigh 5-10kg of tea dead, 3kg of tea, 3kg of big thistle, 1kg of clematis, and wild chrysanthemum 1kg is mixed and pulverized, passed through a 40-mesh sieve, degreased, and then added with 70% ethanol for 3 times of leaching, each leaching for 8-12 hours, respectively undergoing ethanol reflux extraction, filtration, concentration under reduced pressure, freeze-drying and other operations to obtain crude The dry powder is the main active ingredient A of the compound tea dead biopesticide.
[0037] The fresh leaves of Vitex vitex were crushed and refluxed with 70% ethanol at 60°C for 3 times, each time for 8-12 hours, filtered, the extracts were combined, concentrated under reduced pressure, and freeze-dried to obtain the crude dry powder B.
[0038] The culture medium of glucose 50g / L, cottonseed powder 25g / L, corn...
Embodiment 2
[0042] Take the preparation D obtained in Example 1, and prepare tea polyphenol mother solutions of 100 mg / mL, 50 mg / mL, 25 mg / mL, and 0 mg / mL with sterile water, respectively. Add 1mL of the corresponding concentration of mother liquor to a sterile petri dish, then take 9mL of PSA medium cooled to a suitable temperature, mix the two, shake the petri dish immediately until the culture medium and mother liquor are evenly mixed, and wait for it to solidify. PSA medium plates with concentrations of 10 mg / mL, 5 mg / mL, 2.5 mg / mL and 0 mg / mL were obtained. Use a sterile puncher to punch out bacterial discs (φ9mm) at the places where the colony grows vigorously, and then transplant each bacterial disc to the center of each plate. The same amount of sterile water was added to the same amount of medium as a control, and each group was treated 3 times, sealed with plastic wrap and placed in a water-proof constant temperature incubator at 28°C for upside-down culture. Observe the growth...
Embodiment 3
[0046] Get the compound preparation that embodiment 1 gains, be mixed with 20mg / mL liquid medicine in test tube. Use a hole puncher to punch holes in the cultured fungus petri dish, add a fungus disc to each test tube, and culture at 28°C for three days. Take mycelia smears, stain with safranin counterstain immediately after drying, and observe under a microscope. Each group was treated with 3 repetitions. Microscopic observation of the mycelial growth morphology of the treatment ( figure 2 ).
[0047] figure 2 It was shown that in the control group (CK), the hyphae grew intact without obvious abnormalities; in the 20mg / mL treatment group, the hyphae broke into small segments of different sizes. It can thus be shown that the compound preparation can not only inhibit the elongation and germination of mycelium growth, but also lead to bacterial cell rupture and protoplasm leakage.
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