Compound schima superba preparation for resisting rice blast and preparation thereof
A technology for resisting rice blast and wood lotus, which is applied in the fields of botanical equipment and methods, biocides, fungicides, etc. The effect of rich material sources and high anti-disease activity
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Embodiment 1
[0027] 1. Prepare the leaf powder mixture: wash the leaves of Schima superba and the fruits of Sapindia chinensis respectively, put them in air-dry at room temperature, then weigh 15 kg of leaves of Schima superba and 8 kg of fruits of Sapindia sativa, and crush them into powders, mixing them to obtain a powdery mixture;
[0028] 2. 80% ethanol reflux extraction: put the above powder mixture into a container, then pour 115L of 80% ethanol aqueous solution into the container, reflux extraction at 75°C for 5 hours, filter off the residue, and obtain the extract;
[0029] 3. Organic solvent extraction: extract the extract with petroleum ether equal to the volume of the extract to obtain the first raffinate, and then continue to extract the first raffinate with ethyl acetate equal to the volume of the first raffinate. The raffinate is extracted to obtain the second raffinate;
[0030] 4. Preparation preparation: add 2% alkyl polyoxyethylene ether sulfonate in the amount of the se...
Embodiment 2
[0036] 1. Preparation of leaf powder mixture: wash the Schima superba leaves and the tea leaves separately, put them in air-dry at room temperature, then weigh 15kg of the Schima superba leaves and 20kg of the tea leaves, mix them together, and crush them with a pulverizer into powder to obtain the leaf powder mixture;
[0037] 2. 80% ethanol reflux extraction: put the above-mentioned leaf powder mixture into a container, then pour 175L of 80% ethanol aqueous solution into the container, reflux extraction at 75°C for 4 hours, filter off the residue, and obtain the extract;
[0038] 3. Organic solvent extraction: extract the extract with petroleum ether equal to the volume of the extract to obtain the first raffinate, and then continue to extract the first raffinate with ethyl acetate equal to the volume of the first raffinate. The raffinate is extracted to obtain the second raffinate;
[0039] 4. Preparation preparation: add 2% sodium dodecylsulfonate in the second raffinate ...
Embodiment 3
[0041] 1. Preparation of leaf powder mixture: wash the leaves of Schima superba and tea leaves separately, put them in air-dry at room temperature, then weigh 15kg of leaves of Schima superba and 100kg of tea leaves, mix them together, and crush them with a pulverizer into powder to obtain the leaf powder mixture;
[0042]2. 80% ethanol reflux extraction: put the leaf powder mixture into a container, then pour 80% ethanol aqueous solution into the container at a ratio of 1:5, reflux extraction at 75°C for 8 hours, and filter off the residue , to obtain the extract;
[0043] 3. Organic solvent extraction: extract the extract with petroleum ether equal to the volume of the extract to obtain the first raffinate, and then continue to extract the first raffinate with ethyl acetate equal to the volume of the first raffinate. The raffinate is extracted to obtain the second raffinate;
[0044] 4. Preparation preparation: add 5% alkyl polyoxyethylene ether sulfonate in the amount of ...
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