Compound biological pesticide preparation for preventing and treating fruit tree ring rot

A biopesticide and ring disease technology, applied in the direction of biocides, chemicals for biological control, biocides, etc., can solve the problems of low activity, high production cost, short duration of drug effect, etc., and achieve process cost low cost, low price, and easy industrial production

Inactive Publication Date: 2019-04-23
SUZHOU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, botanical pesticides often have disadvantages such as low activity, short duration of efficacy, and high production costs.

Method used

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  • Compound biological pesticide preparation for preventing and treating fruit tree ring rot
  • Compound biological pesticide preparation for preventing and treating fruit tree ring rot
  • Compound biological pesticide preparation for preventing and treating fruit tree ring rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Plant materials such as Chaku, tea leaves, yellow vine roots or stems, Schima superba leaves, Magnolia officinalis leaves, Lily grass, Ginkgo biloba leaves and other plant materials are baked in an oven at 70°C to constant weight, then weigh 50-100g of Chaku and 20-30g of tea , Schima superba leaves 20-30g, Magnolia officinalis fresh leaves 7-10g, Rhymus 20-30g, Ginkgo biloba leaves 7-10g are mixed and crushed, passed through a 40-mesh sieve, degreased, then added 1500-2000mL 70% ethanol to extract 3 times , each extraction for 8-12 hours, respectively, through ethanol reflux extraction, filtration, vacuum concentration, freeze-drying and other operations to obtain rough extraction dry powder, which is the main active ingredient of the compound tea dead biological pesticide.

[0031] The roots or stems of the yellow vine are crushed into coarse powder, extracted three times with 0.2% sulfuric acid solution, 8-12 hours each time, filtered, and the filtrate is collected, a...

Embodiment 2

[0035] Get the main active ingredient of the compound tea dead biological pesticide obtained in Example 1, and dilute it into 5mg / mL, 6.6 mg / mL, 10mg / mL, 13.3mg / mL, 15mg / mL, 20mg / mL according to the extraction mass gradient, and use mycelia Growth rate method was used to determine the inhibitory rate of the extract on the growth of fruit tree ringworm mycelium. Add 1 mL of each of the preparations with 6 concentration gradients to the sterilized petri dishes, and add the same amount of sterile water to the control group, and pour 9 mL of PSA medium at a suitable temperature (just keep your hands in contact with it for a long time) in each petri dish , and immediately shake gently until solidified. Use a puncher to punch out bacterial discs (9 mm in diameter) from the edge of the colonies of 5 vigorously growing fruit tree ringworm bacteria, pick one piece each and move it into the corresponding plate, turn the side with mycelium upside down and place it on the culture medium ...

Embodiment 3

[0039] Take the main active ingredient of the compound tea dead biopesticide obtained in Example 1, dilute it into 5 concentration gradients of 20, 15, 10, 5 and 0 mg / mL according to the extraction mass gradient, and mix well. Use a hole puncher to punch holes in the cultured fungus petri dish, add a fungus disc to each test tube, and culture at 28°C for three days. Take mycelia smears, stain with safranin counterstain immediately after drying, and observe under a microscope. Each group was treated with 3 repetitions. Microscopic observation of the mycelial growth morphology of the treatment ( figure 2 ).

[0040] figure 2 It shows that in the control group (CK), the mycelium grows intact without obvious abnormalities; in the treatment group, with the increase of the concentration of the oil tea meal extract solution, the mycelium gradually swells and breaks; especially in the 20mg / mL treatment group, the hyphae broke into small pieces of different sizes. Thus, it can ...

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Abstract

The invention aims at providing a method which can take tea leaves as main raw materials, mix different plant materials according to a certain proportion, degrease, extract with an ethanol solution, and then prepare composite extract by virtue of filtration, decompression concentration, crystallization and the like. A product of the invention has a remarkable effect for inhibiting the fruit tree ring rot, and also has a strong inhibiting effect for cucumber phytophthora and peach monilinia fructicola.

Description

technical field [0001] The invention belongs to the field of biological pesticides, and relates to a production method and application of a biological pesticide preparation for preventing and treating ring spot of fruit trees such as apples and pears with compound tea dead. Background technique [0002] Apples and pears are one of the most popular fruits in the world. China is the largest producer of apples and pears in the world. At present, my country's apple planting area and total output rank first in the world. The cultivated area, output, export volume and number of varieties of pears also rank among the top in the world. In my country, pears rank third after apples and citrus. [0003] Ring spot disease is also called tumor skin disease and rough skin disease. It is a serious disease in apple and pear producing areas in my country. It is also very common in pear producing areas all over the world. The damage is especially serious in high temperature, high humidity and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/08A01N65/06A01P3/00
CPCA01N65/00A01N65/06A01N65/08
Inventor 陈宏伟陈佳佳钱玮王志伟周颖刘佳李良智
Owner SUZHOU UNIV OF SCI & TECH
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