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Detection method of soyabean phytophthora and special primer

A technology of Phytophthora sojae and test substance, applied in DNA/RNA fragmentation, recombinant DNA technology, fermentation and other directions, can solve the problems of difficult application, low titer of polyclonal antibody antiserum, difficult to reach practical application level, etc. , to achieve the effect of accurate sensitivity

Inactive Publication Date: 2007-01-31
NORTHEAST AGRICULTURAL UNIVERSITY
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Problems solved by technology

In recent years, there have been many reports on the detection of Phytophthora (including Phytophthora soybean) using serological techniques, but there are many difficulties in the practical application of serological methods, mainly because the polyclonal antibody antiserum titer is not high and the specificity is not strong , it is difficult to reach the practical application level; monoclonal antibodies are too specific (maybe at the genus level, or at the species level or even at the physiological race level), and may be missed in practical applications, making it difficult to apply

Method used

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  • Detection method of soyabean phytophthora and special primer
  • Detection method of soyabean phytophthora and special primer
  • Detection method of soyabean phytophthora and special primer

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Embodiment Construction

[0029] 1. Experimental material preparation process

[0030] 1. Experimental strains

[0031] 1.1. Isolation of soil strains

[0032] 1.1.1 Collection location: Soybean field test site of Plant Protection Institute of Heilongjiang Academy of Agricultural Sciences, soybean field test site of Xiangfang Farm, Northeast Agricultural University

[0033] 1.1.2 Collection method: regularly take 3 points in the same soybean field test site, dig out a certain number of soybean plants with complete root systems, put them into sterilized bags, bring them back to the laboratory, shake gently to remove loosely attached roots The soil above was collected with a hairbrush and the remaining soil was the rhizosphere soil. The soil collected at 3 points was mixed as the soil sample of the experimental field, and the fungi, bacteria and actinomycetes in the soil were isolated immediately. At the same time, a part of the rhizosphere soil was stored in a -20°C refrigerator for soil molecular det...

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Abstract

The present invention discloses a detection method of soybean phthoramycin and its special primer. Said special primer is a pair of primers formed from nucleotide sequence of sequence 1 and nucleotide sequence of sequence 2 in sequence table. Said soybean phthoramycin detection method includes the following steps: using genome DNA of material to be detected as template, using the above-mentioned a pair of primers to make PCR amplification and utilizing soybean phthoramycin as positive control, if in the amplification product the identical strip band is existed, said detected material contains soybean phthoramycin.

Description

technical field [0001] The invention relates to a detection method and special primers for Phytophthora soybean. Background technique [0002] Phytophthora sojae can cause Phytophthora sojae root rot, which is extremely harmful in soybean production, and is a Class I dangerous quarantine object announced by China. So far, many research methods have been used for the detection and identification of Phytophthora soybean, but there are many shortcomings in the existing detection technology of Phytophthora soybean, mainly because the detection sensitivity and accuracy are low, the detection time is long, and it is not suitable for quarantine. Departments, especially the requirements for rapid detection at ports. [0003] So far, the classification and identification of plant pathogenic fungi are mainly based on the morphological characteristics of their vegetative bodies and fruiting bodies, and Phytophthora soybean is no exception. The traditional detection and identification...

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Application Information

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IPC IPC(8): C12Q1/68C12P19/34C12N15/11
Inventor 文景芝刘春来杨明秀
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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