Methods and compositions for selecting soybean plants resistant to phytophthora root rot

A soybean plant, resistant technology, applied in the field of plant breeding and disease resistance, can solve the problem of deficiency

Active Publication Date: 2010-03-24
MONSANTO TECH LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, there is a lack of SNP-based marker panels for Rps1 on li

Method used

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  • Methods and compositions for selecting soybean plants resistant to phytophthora root rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0216] Example 1. Variation of SNP markers for introgression of PRR resistance loci when used with haplotypes of known origin of resistance

[0217] Phytophthora soybean is the causative agent of Phytophthora root rot (PRR) and causes significant losses in soybean yields in the United States. Planting resistant varieties is an effective way to control PRR. The breeding of PRR resistant soybean can be greatly facilitated by using marker assisted selection for PRR resistant alleles. Segregating populations were generated to demonstrate SNP markers useful for detection, selection, and introgression of PRR resistance loci into plants.

[0218] Table 1 includes the phenotypic rating scales and definitions used herein for the Examples below. The percentage of surviving plants after P. sojae inoculation was used for classification. This rating provides the basis for all disease ratings and determinations of resistance or susceptibility in the Examples below.

[0219] Table 1: Des...

Embodiment 2

[0247] Example 2: Selection of the Rps1k locus conferring PRR race 4 resistance using SNP markers.

[0248] Using knowledge of PRR resistant germplasm, SNP markers were used to breed PRR resistance (Table 11). F3 populations generated by crosses of two sources of PPR resistance - AG3602 (source of Rps1c) and AG3505 (source of Rps1k) - were screened using two SNP markers. A total of 466 individuals were screened. SNP markers NS0119335 and NS0118166 were used to select for Rps1k (source of PRR race 4 resistance) based on the AG3505 haplotype. Sources of Rps1 resistance include, but are not limited to, AG3602, AG3505, and DKB28-53.

[0249] Table 11. Haplotypes of two sources of PRR resistance.

[0250]

Embodiment 3

[0251] Example 3. Monitoring of introgression of the PRR resistance locus Rps1k using SNP markers.

[0252] F3 individuals produced by crossing two soybean lines AG3602(Rps1c) and AG3505(Rps1k) were genotyped. The Rps1k locus confers resistance to PRR race 4. A total of 466 F3 plants were screened with two SNP markers NS0119335 and NS0118160. Table 12 reports the results of the phenotypic validation of the genotype screen for PRR race 4. Screening with NS0119335 predicted PRR response with 85.7%. Screening with NS0118160 predicted PRR response with 83.8%.

[0253] Table 12. Results of phenotypic screening after haplotype selection based on resistance source.

[0254] mark

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Abstract

The present invention relates to the field of plant breeding and disease resistance. More specifically, the invention includes a method for breeding soybean plants containing quantitative trail loci (QTL) for resistance the Phytophthora root rot (PRR) caused by Phytophthora sojae. The invention further includes the use of molecular markers in the introgression of PRR resistance QTL into soybean plants.

Description

[0001] Cross References to Related Applications [0002] This application claims priority under 35 U.S.C. §119(e) to US Provisional Application 60 / 925,475, filed April 20, 2007, the entire disclosure of which is hereby incorporated by reference. [0003] Introduction of Sequence Listing [0004] The sequence listing was created on April 7, 2008 and contains 52,115 bytes (in Microsoft The file named "Sequence Listing.txt", comprising 116 nucleotide sequences, is hereby incorporated by reference in its entirety. field of invention [0005] This invention is in the field of plant breeding and disease resistance. More specifically, the present invention includes methods of breeding Glycine plants containing quantitative trait loci (QTLs) associated with disease resistance to the pathogen Phytophthora sojae (Kauffman & Gerdemann). The present invention relates to the use of genetic markers to determine QTLs for disease resistance. The invention further relates to the use of...

Claims

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Application Information

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IPC IPC(8): A01H5/10C12Q1/68
CPCC12Q1/6895A01H1/04C12Q2600/172A01H6/542A01H5/10A01H1/045C12Q2600/13C12Q2600/156C12Q2600/16C12N15/8282
Inventor J·贝姆吴坤生J·塔穆朗尼斯V·康西比多J·耶茨
Owner MONSANTO TECH LLC
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