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Application of phytophthora sojae oospore development-related transcription factor Myb gene

A transcription factor, Phytophthora sojae technology, applied in genetic engineering, plant genetic improvement, microorganism-based methods, etc., can solve problems such as differences in the function of Myb proteins

Inactive Publication Date: 2012-11-14
HEFEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the Myb protein has a certain degree of conservation in sequence and a certain similarity in structure, there are also major differences in the function of Myb protein among different species, different individuals, and even different tissues and organs of the same individual.

Method used

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  • Application of phytophthora sojae oospore development-related transcription factor Myb gene
  • Application of phytophthora sojae oospore development-related transcription factor Myb gene
  • Application of phytophthora sojae oospore development-related transcription factor Myb gene

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Experimental program
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Embodiment Construction

[0020] Tested strain

[0021] The tested strain was Phytophthora sojae whole genome sequencing standard strain P26, which was purchased from American Type Culture Collection. The strains were stored in 10% V8 solid medium at 10°C. Soybean cultivar Williams was shown to be susceptible to infection with Phytophthora sojae strain P26, courtesy of the National Soybean Improvement Center of Nanjing Agricultural University.

[0022] Database information and same-origin search

[0023] Phytophthora soybean database (http: / / genome.jgi-psf.org / Physo3 / Physo3.home.html)

[0024] Phytophthora oak database (http: / / genome.jgi-psf.org / Phyra1_1 / Phyra1_1.home.html)

[0025] Phytophthora infestans Database (http: / / www.broad.mit.edu)

[0026] Phytophthora Functional Genome Database (http: / / www.pfgd.org / ).

[0027] Sequence Alignment Analysis

[0028] The resulting Phytophthora sojae gene was compared with the culstal W software on the website of the European Bioinformatics Institute ...

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Abstract

The invention relates to the technical field of molecular biology and gene engineering, in particular to application of a phytophthora sojae oospore development-related transcription factor Myb gene. The phytophthora sojae oospore development-related transcription factor Myb gene has a nucleotide sequence as shown by SEQIDNO.1. A phytophthora sojae Myb transcription factor PsMyb1 gene is the nucleotide sequence as shown by SEQIDNO.1. Protein of the phytophthora sojae Myb transcription factor PsMyb1 gene is a nucleotide sequence as shown by SEQIDNO.2. The PsMyb1 gene provided by the invention mainly aims at regulating and controlling target gene transcription and controlling the development of phytophthora sojae oospores. The gene provided by the invention has a very great value of application to control of phytophthora root rot of soybean. Bactericides which are developed by using the gene as a target have a very important and practical significance to preventing the phytophthora root rot of soybean from occurring and being spread.

Description

technical field [0001] The invention belongs to the field of biological genes, in particular to a transcription factor of Phytophthora soybean Myb PsMyb1 Gene. technical background [0002] Phytophthora sojae ( Phytophthora sojae ) is an extremely important plant pathogen, and the soybean root rot caused by it is one of the devastating diseases in soybean production. The pathogen can infect soybeans at various growth stages of soybeans, and the yield is generally reduced by 10-30% in the year of occurrence, and the yield of severely diseased fields can be reduced by 60%, or even completely lost. Although Phytophthora soybean was first discovered in Northeast my country in the late 1980s, the disease has spread in some parts of my country and has become the main disease in major soybean producing areas such as Northeast, Anhui and Fujian. In some years, it caused Soybean yield losses of up to 50% or more. [0003] People have widely bred resistant varieties to prevent and...

Claims

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Application Information

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IPC IPC(8): C12N15/31C07K14/37C12Q1/68C12R1/645
Inventor 王子迎王朝霞杜家亮
Owner HEFEI NORMAL UNIV
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