Usage of phytophthora sojae oospore development associated transcription factor zinc finger protein gene

A technology of transcription factor and zinc finger protein, applied in genetic engineering, plant genetic improvement, application, etc., can solve the problems of pathogenicity and variability of pathogenic bacteria, loss of utilization value of disease-resistant varieties, loss of soybean yield, etc., and achieve high application value. Effect

Inactive Publication Date: 2012-11-14
HEFEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although Phytophthora soybean was first discovered in Northeast my country in the late 1980s, the disease has spread in some areas of my country and has become the main disease in major soybean producing areas such as Northeast, Anhui and Fujian. In some years, it caused soybean Yield loss up to 50% or more
[0003] People have widely bred disease-resistant varieties to prevent and control the disease, but due to the variable pathogenicity of the pathogen, the emergence of new pathogenic races will easily lead to the loss of the use value of the newly selected disease-resistant varieties

Method used

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  • Usage of phytophthora sojae oospore development associated transcription factor zinc finger protein gene
  • Usage of phytophthora sojae oospore development associated transcription factor zinc finger protein gene
  • Usage of phytophthora sojae oospore development associated transcription factor zinc finger protein gene

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Embodiment Construction

[0019] Tested strain

[0020] Tested strain It is the standard strain P26 for Phytophthora soybean whole genome sequencing, which is preserved by the American Type Culture Collection. The strains were stored in 10% V8 solid medium at 10°C. Soybean cultivar Williams was shown to be susceptible to infection with Phytophthora sojae strain P26, courtesy of the National Soybean Improvement Center of Nanjing Agricultural University.

[0021] Database information and same-origin search

[0022] Phytophthora soybean database (http: / / genome.jgi-psf.org / Physo3 / Physo3.home.html)

[0023] Phytophthora oak database (http: / / genome.jgi-psf.org / Phyra1_1 / Phyra1_1.home.html)

[0024] Phytophthora infestans Database (http: / / www.broad.mit.edu)

[0025] Phytophthora Functional Genome Database (http: / / www.pfgd.org / ).

[0026] Sequence Alignment Analysis

[0027] The resulting Phytophthora sojae gene was compared with the culstal W software on the website of the European Bioinformatics...

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Abstract

The invention relates to the technical field of molecular biology and genetic engineering, in particular to a usage of a phytophthora sojae oospore development associated transcription factor zinc finger protein gene. The zinc finger protein transcription factor Ps-zAl gene of the phytophthora sojae is a nucleotide sequence represented by SEQ ID NO.1, and protein of the zinc finger protein transcription factor Ps-zAl gene of the phytophthora sojae is an amino acid sequence represented by SEQ ID NO.2. The Ps-zAl can mainly control the transcription of target genes and control the development of phytophthora sojae oospores. According to the usage of the phytophthora sojae oospore development associated transcription factor zinc finger protein gene, the gene provided has high application values in the control of root rot of the phytophthora sojae, and bactericides developed with the genes as an action target has large practical significance in the controlling of the occurrence and prevalence of root rot of the phytophthora sojae.

Description

technical field [0001] The invention belongs to the field of biological genes, in particular to a Phytophthora soybean zinc finger protein transcription factor Ps-zfA1 The use of genes. technical background [0002] Phytophthora sojae Phytophthora sojae It is an extremely important plant pathogen, and the soybean root rot caused by it is one of the devastating diseases in soybean production. The pathogen can infect soybeans at various growth stages of soybeans, and the yield is generally reduced by 10-30% in the year of occurrence, and the yield of severely diseased fields can be reduced by 60%, or even completely lost. Although Phytophthora soybean was first discovered in Northeast my country in the late 1980s, the disease has spread in some areas of my country and has become the main disease in major soybean producing areas such as Northeast, Anhui and Fujian. In some years, it caused soybean Yield loss is as high as 50% or more. [0003] People have widely bred resist...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/31C07K14/37A01N61/00A01P3/00
Inventor 王子迎王朝霞杜家亮
Owner HEFEI NORMAL UNIV
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