Paenibacillus polymyxa strain and application thereof

A technology of Paenibacillus polymyxa and bacterial strains, which is applied in the field of microorganisms, can solve the problems such as the lack of biocontrol strains of Paenibacillus polymyxa, and achieve the effects of wide development and application prospects, strong stability, and strong antibacterial effect

Active Publication Date: 2019-05-17
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a lack of Paenibacillus polymyxa biocontrol strains suitable for the control of soybean blight

Method used

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  • Paenibacillus polymyxa strain and application thereof
  • Paenibacillus polymyxa strain and application thereof
  • Paenibacillus polymyxa strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Isolation and identification of Bacillus polymyxa strain YT9

[0032] Soil sample collection: Soybean rhizosphere soil at a depth of 5-10cm was collected from different soybean plots in Yantai City, Shandong Province, brought back to the laboratory, and separated by flat plate dilution coating method.

[0033] Strain isolation medium: The medium is beef extract peptone agar (NA) medium: beef extract 3g, peptone 10g, NaCl 5g, sucrose 15g, agar 20g, add distilled water to dilute to 1000mL, pH7.2-7.4, 121℃ Sterilize for 20 minutes.

[0034] Separation by plate dilution coating method: Mix the collected soil sample thoroughly, weigh 10g, put it into a triangular flask with a proper amount of glass beads and 90mL sterile water, place the triangular flask on a shaker, shake for 20min, and statically Leave it for 5 minutes, and dilute in sequence with a 10-fold gradient dilution method. Take 10 -4 , 10 -5 , 10 -6 200μL each of the soil diluent, evenly spread on the NA pla...

Embodiment 2

[0036] Example 2 Inhibition of Bacillus polymyxa YT9 on different pathogens

[0037] In the center of the V8 solid medium plate, a 6mm diameter of Phytophthora sojae, Phytophthora capsici, Phytophthora tabaci, Rhizoctonia solani, Rhizoctonia solani, Phytophthora sojae, Phytophthora capsici, Rhizoctonia solani, Phytophthora sojae, Phytophthora capsici, Rhizoctonia sp 2.5cm on one side of the bacterial cake was inoculated with the Bacillus polymyxa strain YT9 of the present invention, and a blank control was added to the other side. Each test strain was repeated 5 times and cultured in the dark in a 25°C incubator. According to the growth rate of the pathogenic bacteria 4-8 days later, observe and measure the width of the bacteriostatic zone and determine the bacteriostatic rate. The specific results are shown in Table 1.

[0038] Bacteriostatic rate = (control side colony radius-opposite side colony radius) / control test colony radius * 100%.

[0039] Table 1. Inhibition rate of strai...

Embodiment 3

[0043] Example 3 The effect of aseptic fermentation filtrate of Bacillus polymyxa strain YT9 on the zoospore activity of Phytophthora sojae

[0044] After the strain YT9 was activated, it was inoculated into a 250 mL Erlenmeyer flask containing 50 mL of fermentation medium. The fermentation stock solution was cultured on a shaker at 30°C at 180 rpm for 2 days. After centrifugation at 12000 rpm for 5 minutes, it was filtered with a 0.22 μm bacterial filter. , Obtain aseptic fermentation filtrate. The freshly activated Phytophthora sojae strain was cultured in 10% V8 liquid for two days and then the water was changed to induce a large number of zoospores (10 4 / ML). One group took 450 μl zoospores + 450 μl sterile filtrate + 100 μl V8 (treatment group); the other group took 450 μl zoospores + 450 μl fermentation medium + 100 μl V8 (control group), placed at 25°C for 3 hours and then performed microscopic observation. And each 200μl of the mixed solution was spread on the V8 plate...

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Abstract

The invention discloses a paenibacillus polymyxa strain and application thereof. The strain belongs to paenibacillus polymyxa strains YT9, and the preservation number of the strain is CGMCC NO.15958.The separated strain not only has a strong inhibition effect on phytophthora sojae but also has a great antagonism effect on plant pathogenic oomycetes and phytopathogenic fungi, wherein the plant pathogenic oomycetes include phytophthora capsici, phytophthora nicotianae and the like, and phytopathogenic fungi include wheat sheath blight bacteria, wheat root rot bacteria, cercospora personata, cercospora arachidicola and the like; the bacteriostatic effect on phytophthora sojae is the strongest, wherein the bacteriostatic rate reaches 86.27%+ / -1.62%. In addition, the paenibacillus polymyxa strain YT9 is separated from healthy soybean rhizosphere soil and is free of ecological toxicity, high in safety, wide in bacteriostatic spectrum, stable and lasting in bacteriostatic activity and easy to culture. The strain has a broad application prospect in biological control over plant diseases.

Description

Technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Bacillus polymyxa and its application. Background technique [0002] Soybean blight caused by Phytophthora sojae is one of the devastating diseases in soybean production, causing nearly 2 billion US dollars in economic losses in global soybean production every year. Soybean blight can occur in all stages of soybean growth. It is manifested as sudden seedling death and adult root rot. The disease is particularly serious in wet and rainy seasons. It is easy to cause widespread epidemics and severely affect production, restrict the development of soybean industry, and cause major economic loss. [0003] There are more than 100 species of Phytophthora that have been reported, most of which are plant pathogens that harm agriculture and natural ecosystems (Kroon et al., 2012, The genus Phytophthora anno 2012.), causing varying degrees of harm to a variety of crops and cas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/12
Inventor 盛玉婷张娟姚立强王敏武芳姜春秀郭玉欣
Owner LUDONG UNIVERSITY
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