A strain of Bacillus amyloliquefaciens b4216 and its application

A technology of amylolytic spores, B4216CGMCCNO.18425, applied in the fields of environmental biotechnology and plant disease biological control, which can solve the problems of shortening the fermentation cycle, less research, and accelerating the decomposition of compost substrates, and achieves broad antibacterial spectrum, simple cultivation, and good Antagonistic effect

Active Publication Date: 2021-08-10
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, there are many studies on adding microorganisms or bacterial agents one or more times in the primary fermentation stage, but there are few reports on adding microorganisms or bacterial agents to improve the quality of secondary fermentation compost products in the secondary fermentation stage
At the same time, the purpose of adding microorganisms or bacterial agents to compost is to shorten the fermentation cycle, increase the temperature of compost, accelerate the decomposition of compost matrix, eliminate odor, and degrade antibiotics. For external antifungal active microorganisms to improve the antifungal efficacy of compost products relatively few studies

Method used

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  • A strain of Bacillus amyloliquefaciens b4216 and its application
  • A strain of Bacillus amyloliquefaciens b4216 and its application
  • A strain of Bacillus amyloliquefaciens b4216 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation and identification of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) strain B4216

[0030] Secondary fermentation samples of cow dung compost were collected, and the bacteria were isolated by beef extract peptone medium, and the strains that had inhibitory effect on Fusarium oxysporum f.sp.cubense were initially isolated by confrontation culture.

[0031] The preparation method of the above-mentioned beef extract peptone medium is as follows (take the preparation of 1L medium as an example): 3g beef extract, 10g peptone, 5g NaCl, 20g agar, 1000ml water, pH: 7.4-7.6, sterilized at 121°C for 30 minutes.

[0032] The isolated strain was named strain B4216, and its morphology was as follows: figure 1As shown, it is a rod-shaped Gram-negative bacteria that can form spores. The optimum growth temperature of the strain is about 37°C, and the optimum growth pH range is 5.0-8.8. Extract the genomic DNA of the strain, and use gyrA primers to carry o...

Embodiment 2

[0038] Antagonism of Example 2 bacterial strain B4216 to phytopathogenic fungi

[0039] Botrytis cinereal, Fusarium oxysporum f.sp.cubense, Fusarium oxysporum f.sp.cucumerinum, Phytophthora capsici, Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) strain B4216 was inoculated at a distance of 1.5 cm from the center of the wheat root rot fungus (Bipolarissorokiniana) bacteria cake disc, inoculated in the center of the PDA medium plate, and the control group was not inoculated with B4216, and placed in a dark place at 28 ° C. Photographs were taken after 120 h of cultivation. By observing the inhibition zone to determine whether the strain B4216 has an antagonistic effect on the phytopathogenic fungi, it was found that the bacterial strain B4216 has a good antagonism effect on the above-mentioned phytopathogenic fungi. ( figure 2 )

[0040] The experimental results show that: Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) B4216 has a good antagonistic effect o...

Embodiment 3

[0042] Application of embodiment 3 bacterial strain B4216 in secondary composting

[0043] 1) The bacterial strain B4216 was inoculated in LB liquid medium, and after 48 hours of liquid shake flask culture, the bacteria or spore count was greater than 1 × 10 8 cfu / ml culture solution.

[0044] The culture conditions are: initial natural pH, culture temperature 30° C., shaker 200 rpm for 48 hours. At this time, the bacterial solution contains more than 1 bacterium or spores × 10 8 cfu / ml;

[0045] The preparation method of described LB liquid culture medium is (take configuration 1L culture medium as example):

[0046] Add 10 g of tryptone, 5 g of yeast extract, and 10 g of sodium chloride into 950 ml of deionized water, dilute to 1000 ml with deionized water, and sterilize at 121°C for 20 minutes at natural pH.

[0047] 2) Prepare a mixed compost of chicken manure, cow dung and mushroom dregs (mass ratio 6:5:5) after primary fermentation.

[0048] Prepare the culture br...

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Abstract

The invention discloses a strain of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) B4216, the preservation number of which is CGMCC NO.18425; the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) B4216 provided by the invention has no ecotoxicity, high safety and wide antibacterial spectrum And the cultivation is simple, can proliferate in the compost secondary fermentation heap body and improve the antifungal efficacy of compost; the method for preparing antifungal compost provided by the invention is simple to operate and low in cost, and can effectively improve the antifungal efficacy of compost products. It has a very broad application prospect in the biological control of plant diseases.

Description

technical field [0001] The invention belongs to the fields of environmental biotechnology and biological control of plant diseases, and in particular relates to a microorganism for inhibiting plant pathogenic fungi and its application. Background technique [0002] The use of chemical fertilizers and pesticides has played an important role in the intensive cultivation of agricultural production, and has promoted the development of agriculture to a certain extent. However, in recent years, due to the excessive use of chemical fertilizers and pesticides, problems such as soil compaction, salinization, and soil fertility decline have become increasingly exposed. Excessive chemical fertilizers and pesticides have disturbed the soil microbial flora, reduced beneficial organisms, and soil-borne diseases of crops year by year aggravated. Soil-borne diseases refer to diseases caused by pathogens living in the soil that attack crops from the root or stem base of the crop when the co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F17/20C05G3/60A01N63/22A61P3/00C12R1/07
CPCC05G3/00C12N1/20C12N1/205C12R2001/07Y02W30/40
Inventor 孙宪昀赵彬涵李少杰张振颖
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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