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A strain of Bacillus mohewei b282 and its application

A technology of Bacillus vinifera and B282, which is applied in the direction of application, bacteria, and fungicides, can solve the problems of less research, shorten the fermentation cycle, and accelerate the decomposition of compost substrates, and achieve simple cultivation, wide antibacterial spectrum, and good antagonistic effect. Effect

Active Publication Date: 2021-04-27
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, there are many studies on adding microorganisms or bacterial agents one or more times in the primary fermentation stage, but there are few reports on adding microorganisms or bacterial agents to improve the quality of secondary fermentation compost products in the secondary fermentation stage
At the same time, the purpose of adding microorganisms or bacterial agents to compost is to shorten the fermentation cycle, increase the temperature of compost, accelerate the decomposition of compost matrix, eliminate odor, and degrade antibiotics. For external antifungal active microorganisms to improve the antifungal efficacy of compost products relatively few studies

Method used

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  • A strain of Bacillus mohewei b282 and its application
  • A strain of Bacillus mohewei b282 and its application
  • A strain of Bacillus mohewei b282 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Bacillus mohaiwei ( Bacillus mojavensis ) isolation and identification of strain B282

[0028] The second fermentation samples of cow dung compost were collected, and the bacteria were isolated by using beef extract peptone medium, and the bacteria against banana Fusarium wilt ( Fusariumoxysporumf.sp. cubeenseFusariumoxysporumf.sp. cubes ) strains with inhibitory effect.

[0029] The preparation method of the above-mentioned beef extract peptone medium is as follows (taking the preparation of 1 L medium as an example): 3 g beef extract, 10 g peptone, 5 g NaCl, 20 g agar, 1000 ml water, pH: 7.4-7.6, extinguished at 121 °C Bacteria for 30 minutes.

[0030] The isolated strain was named strain B282, and its morphology was as follows: figure 1 As shown, it is a rod-shaped Gram-negative bacteria that can form spores. The optimum growth temperature of the strain is about 37°C, and the optimum growth pH range is 5.0-8.8. Genomic DNA of the strain was extracted, and 1...

Embodiment 2

[0041] Antifungal effect of strain B282

[0042] Botrytis cinerea ( Botrytis cinereal ), Fusarium wilt of banana ( Fusariumoxysporumf.sp. cubeenseFusariumoxysporumf.sp.cubense ), Fusarium wilt of cucumber ( Fusarium oxysporum f.sp. Cucumerinum ), Phytophthora capsici ( Phytophthoracapsici ), wheat root rot ( Bipolarissorokiniana ) bacteria cake disk, inoculated in the center of the PDA medium plate, and inoculated Bacillus mohaiwei at a distance of 1.5 cm from the center ( Bacillus mojavensis ) strain B282, the control group was not inoculated with B282, cultured in the dark at 28°C for 4 days and photographed. By observing the inhibition zone to determine whether the strain B282 has an antagonistic effect on the phytopathogenic fungi, it is found that the bacterial strain B282 has a good antagonism effect on the above-mentioned phytopathogenic fungi. ( figure 2 )

[0043] The experimental results showed that: Bacillus mohaiwei ( Bacillus mojavensis ) B282 ha...

Embodiment 3

[0046] Methods and verification of improving antifungal efficacy of compost 1) Inoculate antifungal active microorganisms (such as strain B282) in LB liquid medium, and culture them in liquid shake flasks for 48 hours to obtain bacteria or spores greater than 1×10 8 cfu / ml culture solution.

[0047] The culture conditions are: initial natural pH, culture temperature 30° C., shaker 200 rpm for 48 h. At this time, the amount of bacteria or spores in the bacterial solution is greater than 1×10 8 cfu / ml;

[0048] The preparation method of the LB liquid medium is as follows (take the configuration of 1 L medium as an example): add 10 g of tryptone, 5 g of yeast extract, and 10 g of sodium chloride in 950 ml of deionized water, and set Make up to 1000 ml, natural pH, sterilize at 121°C for 20 min.

[0049] 2) Prepare a mixed compost of chicken manure, cow dung and mushroom dregs (mass ratio 6:5:5) after primary fermentation.

[0050] Prepare the culture broth of the antifungal a...

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Abstract

The invention discloses a strain of Bacillus mojavensis (Bacillus mojavensis) B282, the preservation number of which is CGMCC NO.17656; Application in antifungal compost; the invention also discloses a method for preparing antifungal compost, which is to prepare high-efficiency antifungal compost by adding antifungal active microorganisms to the compost secondary fermentation compost and relying on their proliferation. The Bacillus mojavensis B282 provided by the present invention has no ecotoxicity, high safety, broad antibacterial spectrum and simple cultivation, can proliferate in the compost secondary fermentation pile and improve the antifungal efficacy of the compost; The method for preparing antifungal compost provided by the invention is simple in operation and low in cost, can effectively improve the antifungal efficacy of compost products, and has very wide application prospects in biological control of plant diseases.

Description

technical field [0001] The invention belongs to the technical field of environmental biotechnology and plant disease biological control, and specifically relates to the technical field of microorganisms for inhibiting fungi. Background technique [0002] The use of chemical fertilizers and pesticides has played an important role in the intensive cultivation of agricultural production, and has promoted the development of agriculture to a certain extent. However, in recent years, due to the excessive use of chemical fertilizers and pesticides, problems such as soil compaction, salinization, and soil fertility decline have become increasingly exposed. Excessive chemical fertilizers and pesticides have disturbed the soil microbial flora, reduced beneficial organisms, and soil-borne diseases of crops year by year aggravated. Soil-borne diseases refer to diseases caused by pathogens living in the soil that attack crops from the root or stem base of the crop when the conditions ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/60A01P3/00C12R1/07
CPCC05G3/00C12N1/205C12R2001/07
Inventor 孙宪昀赵彬涵李少杰张振颖
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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