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A strain of Paenibacillus polymyxa and its application

A technology of Paenibacillus polymyxa and bacilli, applied in the field of microorganisms, can solve the problems of lack of biocontrol strains of Paenibacillus polymyxa, etc., and achieve the effects of wide development and application prospects, high safety, and strong antibacterial effect

Active Publication Date: 2019-07-23
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a lack of Paenibacillus polymyxa biocontrol strains suitable for the control of soybean blight

Method used

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  • A strain of Paenibacillus polymyxa and its application
  • A strain of Paenibacillus polymyxa and its application
  • A strain of Paenibacillus polymyxa and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Isolation and identification of Paenibacillus polymyxa strain YT9

[0032] Soil sample collection: Soybean rhizosphere soil at a depth of 5-10cm was collected from different soybean plots in Yantai City, Shandong Province, brought back to the laboratory, and separated by flat plate dilution coating method.

[0033] Strain isolation medium: the medium is beef extract peptone agar (NA) medium: beef extract 3g, peptone 10g, NaCl 5g, sucrose 15g, agar 20g, add distilled water to 1000mL, pH7.2-7.4, 121℃ Sterilize for 20 minutes.

[0034] Separation by plate dilution coating method: fully mix the collected soil samples, weigh 10g, put into a conical flask with an appropriate amount of glass beads and 90mL sterile water, place the conical flask on a shaker, vibrate for 20min, and Place for 5min, and dilute sequentially by 10-fold gradient dilution method, take 10-4 、10 -5 、10 -6 Each 200µL soil dilution was evenly spread on the NA plate. Each dilution was repeat...

Embodiment 2

[0036] Example 2 Inhibition of Paenibacillus polymyxa YT9 to different pathogenic bacteria

[0037] In the center of the V8 solid medium plate, connect different pathogenic bacteria cakes such as Phytophthora soybean, Phytophthora capsici, Phytophthora tobacco, wheat sheath blight, wheat root rot, peanut black spot, and peanut brown spot with a diameter of 6mm. Paenibacillus polymyxa strain YT9 of the present invention was connected at 2.5 cm on one side of the bacteria cake, and a blank control was added on the other side. Each test strain was repeated 5 times, and placed in a 25° C. incubator for dark cultivation. According to the growth rate of pathogenic bacteria after 4-8 days, observe and measure the width of the bacteriostatic zone, and determine the bacteriostatic rate. The specific results are shown in Table 1.

[0038] Bacterial inhibition rate = (control side colony radius - confrontation side colony radius) / control test colony radius * 100%.

[0039] Table 1. In...

Embodiment 3

[0043] Example 3 Effect of Paenibacillus polymyxa strain YT9 aseptic fermentation filtrate on zoospore activity of Phytophthora soybean

[0044] After activating the strain YT9, inoculate it into a 250mL Erlenmeyer flask containing 50mL of fermentation medium, shake and cultivate the fermentation stock solution for 2 days on a shaking table with a rotation speed of 180rpm at 30°C, centrifuge at 12000rpm for 5min, and filter it with a 0.22μm bacterial filter , to obtain a sterile fermentation filtrate. Freshly activated Phytophthora sojae strains were cultured in 10% V8 liquid for two days, and then the water was changed to induce a large number of zoospores (10 4 individual / mL). One group took 450 μl zoospores + 450 μl sterile filtrate + 100 μl V8 (treatment group); the other group took 450 μl zoospores + 450 μl fermentation medium + 100 μl V8 (control group), and placed them at 25°C for 3 hours for microscopic observation. 200 μl of the mixed solution was taken and spread ...

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Abstract

The invention discloses a strain of Paenibacillus polymyxa and application thereof. The strain is Paenibacillus polymyxa strain YT9, and the preservation number is CGMCC NO.15958. The bacterial strain isolated by the present invention not only has a strong inhibitory effect on Phytophthora soybean, but also has a strong inhibitory effect on plant pathogenic oomycetes such as Phytophthora capsici and Phytophthora tobacco, and wheat sheath blight, wheat root rot, peanut black spot, peanut Phytopathogenic fungi such as brown spot fungus have strong antagonistic effects, and the antibacterial effect on Phytophthora soybean is the strongest, as high as 86.27%±1.62%. In addition, the Paenibacillus polymyxa YT9 strain of the present invention is isolated from healthy soybean rhizosphere soil, has no ecotoxicity, high safety, broad antibacterial spectrum, stable and long-lasting antibacterial activity, and simple cultivation, and has great potential in the biological control of plant diseases. Very broad application prospects.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Paenibacillus polymyxa and its application. Background technique [0002] Soybean blight caused by Phytophthora sojae is one of the most devastating diseases in soybean production, causing economic losses of nearly 2 billion US dollars in global soybean production every year. Soybean blight can occur in all stages of soybean growth, manifested as sudden death of seedlings and root rot of adult plants. The disease is particularly serious in wet and rainy seasons, and it is easy to cause large-scale epidemics, which seriously affects production, restricts the development of the soybean industry, and causes major economic losses. loss. [0003] There are more than 100 species of Phytophthora reported so far, most of which are plant pathogenic bacteria that endanger agriculture and natural ecosystems (Kroon et al., 2012, The genus Phytophthora anno 2012.), causi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/12
Inventor 盛玉婷张娟姚立强王敏武芳姜春秀郭玉欣
Owner LUDONG UNIVERSITY
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