47 results about "Cercospora" patented technology

The present invention relates to the field of plant breeding. More specifically, the present invention includes a method of using haploid plants for genetic mapping of traits such as disease resistance. Further, the invention includes a method for breeding corn plants containing quantitative trait loci (QTL) that are associated with resistance to Gray Leaf Spot (GLS), a fungal disease associated with Cercospora spp. The invention further includes a method for breeding corn plants containing QTL that are associated with Goss' Wilt, a bacterial disease associated with Clavibacter michiganense spp.

The present invention is in the field of plant breeding and disease resistance. More specifically, the invention includes a method for breeding corn plants containing quantitative trait loci that are associated with resistance to gray leaf spot, a fungal disease associated with Cercospora spp. The invention further includes germplasm and the use of germplasm containing quantitative trait loci (QTL) conferring disease resistance for introgression into elite germplasm in a breeding program for resistance to gray leaf spot.

The invention belongs to the field of biotechnology, and provides an isolation method for highly parasitic pathogenic fungi, for study in the future. Single spore isolation is an effective method for isolating the highly parasitic pathogenic fungi, and is particularly suitable for isolating the pathogenic fungi that suffer severe pollution caused by isolation with a conventional method and are difficult to isolate successfully, namely, venturia nashicola, marssonina mali and cercospora chionea. A conventional single spore isolation technology is complex in operation and high in technical requirements, thereby restricting applications thereof. In the isolation method provided by the invention, by punching holes with capillary tubes having the diameter of 1 mm, an operation process is greatly simplified; operability of single spore isolation is stronger; and speed is faster. A general technical staff can master the technology through simple training, thereby broadening an application scope of the method. A good effect can be achieved by mainly adopting the method to isolate the highly parasitic pathogenic fungi and ascospore.

The present invention belongs to the field of biological prevention and control and specifically discloses a bacillus velezensis strain HMB28023. The deposit number of the bacillus velezensis strain is CGMCC No.19002. The present invention also discloses a microbial agent containing the strain. The HMB 28023 strain has a prevention effect on bortrytis cinerea of 84.39% or more, is wide in prevention and control spectrum, has the high prevention effect on the bortrytis cinerea, also has very good prevention and control effects on sphaeropsis sapinea, botryospuaeria berengeriana, valsa sordida,dothiorella gregaria and cercospora insulana, and is highly specialized for the bortrytis cinerea and not liable to produce drug resistance. A microbial inoculant is safe for humans and animals and has no environmental pollution problems.

The invention relates to an agriculture agent, in particular to a mixed chemical for controlling cercospora black spot of peanut and a control method of cercospora black spot of peanut. The chemical for controlling the cercospora black spot of peanut of the invention comprises diniconazole, carbendazol and palmityl alcohol which are mixed evenly in proportion by weight of 1:1:1-2. Proven by testing, the combination bactericide of the invention has stable chemical property and obvious synergism, and presents obvious synergism and complementary effects on the controlled object.

Disease in plants caused by infection with cercosporin producing strains of Cercospora may be controlled by application of laccase. When applied to the locus of the plant, or parts or seed thereof, the cercosporin toxin produced by this pathogen is degraded by the enzyme, thereby preventing or minimizing damage to the plant. The laccase may be applied alone, or in combination with one or more laccase-producing microorganisms.

The invention relates to a method for simply and quickly separating cercospora monospores. A specially-made spore taking device is adopted, one end of the spore taking device is made into a flat round spore taking ball, and the other end of the spore taking device is made into a spore taking shovel which is slightly sharp at the front end and is in a flat shovel shape. The spore taking device can glue pathogen spores of a small amount, a culture medium is evenly coated with the pathogen spores, it can be guaranteed that one spore arises in a view of 10*10 times, the spore density is low, a target spore is easy to find, blade residual bodies do not adhere easily, the pathogen spores can be separated easily, and the separation rate and separation speed are improved effectively. The chosen spore is a spore which is cultured for 24 h and is germinated, the result that germination cannot occur after single spores are chosen in other methods is effectively avoided, and the success rate is high. The spore is chosen in a mode of spore taking through adhesion, the operation is quick, the success rate is high, the picking efficiency is improved, the operation technique difficulty of the method is not high, the method can be mastered skillfully after several trainings, and the method is easy to handle, simple and easy to learn.

The invention belongs to the field of agricultural bactericides and herbicides, and particularly discloses triazole Mannich base compounds containing trifluoromethyl and piperazine, as well as preparation methods and application of the triazole Mannich base compounds. The triazole Mannich base compounds have structural formulas shown in formula I and formula II; in the structural formulas, R1 and R2 are defined as claim 1. The triazole Mannich base compounds have certain in-vitro inhibitory activities on such plant pathogens as cucumber fusarium wilt pathogens, peanut cercospora brown spot pathogens, apple ring spot pathogens, tomato early blight pathogens, wheat sharp eyespot pathogens and wheat scab pathogens, and particularly has relatively high in-vitro inhibitory activities on the apple ring spot pathogens and the wheat sharp eyespot pathogens. The triazole Mannich base compounds with the structural formulas shown in formula I and formula II both have certain weeding activities on oilseed rapes, and have relatively high in-vitro inhibitory activities on ketol-acid reductoisome-rase (KARI) of rice. The triazole Mannich base compounds are applicable to comprehensive control of the damage caused by bacteria and weeds, and inhibition on the KARI activity.

This invention encompasses the identification and isolation of genes that confer disease control properties in plants, as well as plants comprising such genes. These genes are derived from the following sources: Nicotiana benthamiana, Oryzae sativa (var. Indica IR7), Papaver rhoeas, Saccharomyces cerevisiae and Trichoderma harzianum (Rifai 1295-22). The control conferred is against the one or more of the following phytopathogens: Aspergillus flavus, Cercospora zeae-maydis, Fusarium monilforme, Fusarium graminearum, Helminthosporium maydis, Phoma lingam, Phomopsis helianthi, Phytopthera infestans, Pyricularia oryzae, Pythium ultimum, Rhizoctonia solani, Sclerotinia sclerotiorum, Ustilago maydis, and Verticillium dahliae. Further, this invention encompasses other homologous and heterologous sequences with a high degree of functional similarity.

The invention belongs to the field of biotechnology, and provides a method for separating pathogenic fungi, in order to facilitate future research. Single spore isolation is the most effective method for separating parasitic pathogenic fungi, particularly suitable for the separation of pathogenic fungi, which are seriously polluted and difficult to separate by a traditional tissue isolation method, such as Venturia nashicola, apple Marssonina coronaria and Cercospora fungus. The traditional single spore isolation technique has complex operation, high technical requirements, and limited application. Employing capillary with diameter of 1 mm for drilling greatly simplifies the operation process, realizes strong operability of single spore isolation and higher speed; and general technical personnel can grasp the technology through simple training, so as to expand the scope of application. The method is mainly used for the separation of parasitic fungi and ascospores and gains good effect.

The invention belongs to the field of biotechnology, in particular to a biopesticide for controlling cercospora varrireolorWinter, and a preparation method for the biopesticide. Through the compounding of bacillus subtilis HS5B5 which has the antagonism to pathogenic fungi of cercospora varrireolorWinter, biological fertilizer and a Bordeaux mixture, growth and reproduction of cladosporium paeoniae can be effectively inhibited, so that the purpose of cercospora varrireolorWinter control can be realized; meanwhile, the Bordeaux mixture also has the inhibiting effect on other fungus diseases of peony, so that the control effect on the fungus diseases of peony is improved after the Bordeaux mixture is compounded with bacillus subtilis and the biological fertilizer. According to the biopesticide, as the biological fertilizer and the Bordeaux mixture are added into the bacillus subtilis HS5B5, the florescence is prolonged by 2-3 days averagely as compared with that of conventional planting. The biopesticide is adopted to control cercospora varrireolorWinter, and is safe to people and animals, free of environment pollution, efficient and broad in spectrum, and the possibility of drug resistance generation is low.

The invention discloses a sterilization composition containing cyprodinil and prochloraz, the effective constituents are the cyprodinil and the prochloraz, and the weight ratio of the cyprodinil and the prochloraz in the composition is (50:1)-(1:50); the total weight of the cyprodinil and the prochloraz accounts for 0.1%-0.8% of the weight of the composition, and the balance is auxiliaries; afterthe cyprodinil and the prochloraz are compounded at certain proportion, the synergism can be provided for indoor activity of the Sclerotium rolfsii Sacc., and the sterilization composition is suitablefor preventing and curing the peanut southern blight caused by the Selerotium rolfsii Sacc. and can also be suitable for preventing and curing other crop diseases such as peanut cercospora brown spotdisease, potato tarspot and rice sheath blight disease.

The invention discloses paenibacillus polymyxa for resisting multiple peony pathogenic bacteria and an application of paenibacillus polymyxa. The paenibacillus polymyxa for resisting multiple peony pathogenic bacteria is paenibacillus polymyxa CSM1101, and the preservation number of paenibacillus polymyxa in the China General Microbiological Culture Collection Center is CGMCC No.8527. The paenibacillus polymyxa CSM1101 can remarkably inhibit Botrytis paeoniae, Cladosporium paeoniae and Cercospora varricolor Winter, and can remarkably promote growth of peony.

The invention relates to two optically pure isomers of NK007 for short and applications in a bactericidal aspect. Compounds NK007-3 and NK007-4 have good bactericidal activity, can be used for treating plant bacteria diseases selected from fusarium wilt of cucumber, cercospora brown spot of peanut, apple ring spot, alternaria solani, puccinia polysora, rice bakanae disease, sclerotinia rot of colza, phytophthora capsici disease, wheat sharp eyespot, bipolaris maydis, watermelon anthracnose, potato late blight, rice sheath blight disease and cucumber gray mold.

The invention discloses a microbial herbicide. The microbial herbicide is prepared from, by weight, 1-3 parts of pseudomonas, 2-3 parts of sclerotinia scleroterum, 3-7 parts of cercospora, 5-10 parts of aminoacetylate, 20-30 parts of alcohol, 2-5 parts of sodium dodecyl benzene sulfonate, metolachlor 1-5 parts of metolachlor, 1-2 parts of polytrisiloxane, 2-3 parts of polyoxyethylenesorbitan monooleate sorbic alcohol, and 3-7 parts of adsorption carrier. The microbial herbicide has the advantages that the weed in rapid growth is controlled according to the principle of ecology; the the microbial herbicide is natural, environmentally friendly, less in consumption without any toxicity or side effects; thus the damage to the environment is reduced without effect on the safety of crop, and metabolites can also be absorbed as crop nutrition to realize the benign circulation.

A biological inoculant having both fertilizing and fungicidal activity is disclosed. More particularly, a bio-inoculant having the said combined effects, comprising Bradyrhizobium japonicum and specific isolates from the Trichoderma genus is disclosed. The bio-inoculant is applied to soybean crops for preventing fungi borne diseases. More particularly, the bio-inoculant of the invention is useful for protecting soybean crops against infection by Fusarium sp., Colletotrichum sp., Cercospora sp., Sclerotinia sp. and Rhizoctonia sp.

The invention provides a planting method of sugarcane. The planting method comprises the steps of ditching, field planting and application of special-purpose base fertilizer which is prepared by conducting culture with bagasse, soybean curd residues, peptone, water, a cercospora achyranthis strain, a cercospora ageratum conyzoides strain, a bacillus subtilis strain and a lactobacillus reuteri strain for 20-30 days, and then mixing a culture product with 5,000 kg of organic fertilizer. By means of the method, the problem that weeds and the sugarcane compete for water and fertilizer can be effectively solved, the use of chemical fertilizer and pesticide can also be reduced, the gas permeability of soil is improved, and the growth of the sugarcane is promoted.

The present invention relates to a cercospora edulis specific molecular detection primer and application thereof, the cercospora edulis specific molecular detection primer includes an upstream primerand a downstream primer as shown in sequence tables SEQ ID No.1-2. The cercospora edulis specific molecular detection primer pair can amplify specifically in cercospora edulis to obtain a target product of 769 bp. The application comprises application of the cercospora edulis specific molecular detection primer in detection of soybean Cercospora sojina Hara and preparation of a detection kit for detecting the soybean Cercospora sojina Hara. The primer has high specificity and can be used for distinguishing soybean sclerotinia blight, Soybean Phytophthora Root, Soybean purple blotch, soybean gibberellic disease, soybean wilt and other soybean common disease pathogenic bacteria, and has high sensitivity, the detection sensitivity of cercospora edulis genomic DNA is up to 1ng / mu L; the usemethod is simple and quick, and cloning, sequencing, enzyme digestion and other operation are not needed for PCR products.

The invention discloses a compound composition comprising isolongifolene ketoxime lactam and trichloroisocyanuric acid and a bactericide. The compound composition comprises isolongifolene ketoxime lactam and trichloroisocyanuric acid at a weight ratio of (20-1):(1-20). The weight sum of active ingredients A and B accounts for 0.1-90% of the total weight of the composition. The compound compositionshows an obvious synergetic effect; the dosage of the active ingredients is reduced; and at the same time, the bactericide resistance of diseases is reduced. The composition can be used for preventing and controlling various crop diseases such as citrus canker, tomato bacterial wilt, melon fusarium wilt and peanut cercospora leaf spot.