24 results about "Phytoene dehydrogenase" patented technology

The present invention relates to the field of genetic engineering technology, in particular to taro CePDS Gene and VIGS virus vector and silencing method thereof. The present invention provides a taro phytoene dehydrogenase, the amino acid sequence of which is shown in SEQ ID NO.1. The present invention discovered the amino acid sequence of taro phytoene dehydrogenase and its encoding gene CePDS sequence, on this basis, provides a kind of silencing CePDS The target sequence of the gene, the VIGS silencing system and the silencing method can effectively reduce the CePDS The expression level of the gene can enable the VIGS silencing system to spread systemically in taro plants, thereby effectively interfering with the CePDS The VIGS silencing system and method provided by the present invention will greatly improve the progress of taro gene function research and significantly save time and cost.

The invention relates to an application of a genetic transformation method of populus sibirica and a CRISPR / Cas9 gene editing method in plant breeding, and belongs to the technical field of plant molecular breeding. The method comprises the following steps: (1) soaking an explant in an infection bacterium solution; (2) spreading on a CM1 culture medium; (3) transferring the explant to a CM2 culture medium for 4-5 weeks; (4) transferring the calluses to a CM3 culture medium until adventitious buds are obtained; (5) transferring the callus with the adventitious buds to a CM4 culture medium until cluster buds are obtained; and (6) cutting off clustered seedlings, and inserting the clustered seedlings on a CM5 culture medium. According to the application of the genetic transformation and CRISPR / Cas9 gene editing method of the poplar in plant breeding, a target gene is transferred into a poplar body to obtain a positive plant, meanwhile, a poplar phytoene dehydrogenase (PDS) gene is taken as an example, a poplar mutant of which the PDS gene is knocked out is successfully obtained by applying a CRISPR / Cas9 gene editing technology, and the method is suitable for plant breeding. And a technical support is provided for application of a CRISPR / Cas9 gene editing technology in molecular breeding of the populus sibirica.

The invention discloses a saccharomyces cerevisiae strain for de novo synthesis of fucoxanthine precursor neoxanthine and application of the saccharomyces cerevisiae strain, and belongs to the technical field of biology. According to the recombinant saccharomyces cerevisiae disclosed by the invention, the 3-hydroxy-3-methylglutaryl coenzyme A reductase, the isopentenyl pyrophosphate isomerase, the farnesyl pyrophosphate synthase and the endoplasmic reticulum size regulation factor are expressed in an enhanced manner; according to the method, geranyl geranyl diphosphate synthase, phytoene dehydrogenase, 15-cis-phytoene synthase, bifunctional lycopene cyclase / phytoene synthase, beta-carotene hydroxylase, zeaxanthin epoxidase truncated mutant and violaxanthin deep oxidase-like protein are subjected to heterologous expression, so that the zeaxanthin deep oxidase-like protein is obtained; the ROX1 and GAL80 genes in the saccharomyces cerevisiae are knocked out, and the expression of squalene synthase ERG9 is down-regulated. When the strain is subjected to shake flask fermentation, the contents of neoxanthine and violaxanthin of the strain respectively reach 1.78 mg / L and 26.77 mg / L, so that the strain has a wide application prospect.

The invention belongs to the field of microorganisms, and relates to an expression cassette for producing microbial metabolites. Specifically, the invention relates to an expression cassette for expressing beta-carotene, which comprises the following elements: (1) a promoter; (2) a polynucleotide encoding a phytoene synthase; (3) a polynucleotide encoding a phytoene dehydrogenase; (4) a polynucleotide encoding a lycopene cyclase; (5) a polynucleotide encoding a geranyl and geranyl pyrophosphate synthase; and (6) a terminator. The invention also relates to a combination of expression cassettes comprising said expression cassette, a vector, a host cell, a recombinant microorganism, and the use of said expression cassette for producing a microbial metabolite.

The invention relates to the technical field of gene engineering, in particular to a cynanchum thesioides CtPDS gene as well as a VIGS virus vector and a silencing method of the cynanchum thesioides CtPDS gene. The invention provides phytoene dehydrogenase of cynanchum thesioides. The phytoene dehydrogenase has an amino acid sequence as shown in SEQ ID NO. 1. The invention discloses an amino acid sequence of cynanchum thesioides phytoene dehydrogenase and a sequence of a coding gene CtPDS of the cynanchum thesioides phytoene dehydrogenase, and provides a target nucleic acid molecule for silencing the CtPDS gene, a VIGS silencing system and a silencing method on the basis of the amino acid sequence of the cynanchum thesioides phytoene dehydrogenase. The leaf CtPDS gene expression level of a CtPDS gene silent plant constructed by the method provided by the invention is remarkably reduced, the silence efficiency reaches 56-65%, the chlorophyll and carotenoid contents are remarkably reduced, the leaf has a whitening phenotype, and a technical support is provided for developing functional research of cynanchum thesioides key genes.

The invention discloses yarrowia lipolytica with high yield of beta-carotene as well as a construction method and application of the yarrowia lipolytica. According to the yarrowia lipolytica engineering bacterium, geranyl diphosphate synthase gene xdGPS, phytoene dehydrogenase gene CarB, phytoene cyclization / phytoene synthesis bifunctional enzyme mutant gene GarRPY27R, acetyl-coenzyme A synthetase mutant gene ACSL641P and pyruvate ferredoxin oxidoreductase gene nifJ are integrated and expressed on a chromosome, and the yarrowia lipolytica engineering bacterium is obtained. Meanwhile, a 3-hydroxy-3-methylglutaryl CoA reductase gene HMGR (3-hydroxy-3-methylglutaryl CoA reductase) derived from the yarrowia lipolytica strain is subjected to overexpression; the yield of the beta-carotene is increased to 13.186 g / L, and the production efficiency is 0.14 g / L / h.

The invention provides a wild barley phytoene dehydrogenase gene HbPDS, a specific fragment and application of the wild barley phytoene dehydrogenase gene HbPDS. The HbPDS gene specific fragment and the recombinant virus vector pTRV2-HbPDS are constructed, a wild barley HbPDS gene VIGS silencing system is established for the first time, the phytoene dehydrogenase gene HbPDS of the wild barley can be silenced, synthesis of carotenoid of the wild barley is blocked, and the phenomena of chlorophyll fading, leaf color whitening and the like of the wild barley are caused.

The present invention relates to the use of phytoene dehydrogenase (PDS), said method for controlling weeds comprising applying a herbicide containing an effective dose of phytoene dehydrogenase inhibitor (PDSi) to a field in the presence of at least one transgenic plant, the present invention relates to a transgenic plant comprising a polynucleotide sequence encoding phytoene dehydrogenase in its genome, said transgenic plant having reduced plant damage and / or increased plant yield compared to other plants that do not have a polynucleotide sequence encoding phytoene dehydrogenase. The phytoene dehydrogenase PDS1, the phytoene dehydrogenase PDS3, the phytoene dehydrogenase PDS4, the phytoene dehydrogenase PDS5, the phytoene dehydrogenase PDS6, the phytoene dehydrogenase PDS7 and the phytoene dehydrogenase PDS8 have high tolerance to PDS inhibitor herbicides, plants containing the polynucleotide sequence for coding the phytoene dehydrogenase have high tolerance to the PDS inhibitor herbicides, and the phytoene dehydrogenase has high tolerance to diflufenican and diflufenican with the 4-time field concentration. Therefore, the application prospect on plants is wide.

The invention relates to the field of astaxanthin synthesis, and discloses recombinant saccharomyces cerevisiae as well as a construction method and application thereof. The recombinant saccharomyces cerevisiae provided by the invention comprises the following exogenous genes: coding genes of beta-carotene hydroxylase CrtZ, beta-carotene ketolase CrtW, nicotinamide adenine dinucleotide kinase, ferredoxin-NADPH (nicotinamide adenine dinucleotide phosphate) oxidoreductase and ferredoxin; on the basis of the gene, other related genes are further introduced to realize that CrtZ and CrtW are positioned in lipid droplets by lipid droplet targeting peptide Olesion, so that the yield of astaxanthin is increased, finally geranyl-geranyl diphosphate synthase, phytoene dehydrogenase and phytoene synthase are targeted to endoplasmic reticulum through endoplasmic reticulum targeting peptide, and the yield of astaxanthin is increased. Therefore, the yield of the fermentation tank is up to 440mg / L. The recombinant saccharomyces cerevisiae provided by the invention is clear in genetic background, can stably and efficiently produce astaxanthin, and has a wide application prospect.

The present invention discloses a method for constructing and applying an engineered Trichoderma reesei strain capable of producing lycopene, belonging to the field of bioengineering. This construction method uses the filamentous fungus Trichoderma reesei, which does not produce lycopene, or a derivative thereof, as a starting strain, and introduces exogenous genes encoding a single-function mutant of phytoene synthase and a phytoene dehydrogenase to obtain an engineered strain. Through genetic modification, the present invention obtains an engineered Trichoderma reesei strain capable of producing lycopene. The engineered strain uses glucose as a carbon source for direct fermentation to produce lycopene; it also uses lactose and cellulose as carbon sources for simultaneous fermentation to produce lycopene and cellulase. This engineered strain achieves dual production of enzymes and lycopene in a single fermentation process, increasing product profits and reducing production costs. This method also provides a new method for microbial production of lycopene, which can be applied to the industrial production of lycopene and cellulase.

This invention discloses an engineered strain of *Yersinia lipolytica* for synthesizing astaxanthin, its preparation method and application, a method for producing astaxanthin, and a method for increasing astaxanthin yield. The engineered strain of *Yersinia lipolytica* for synthesizing astaxanthin is obtained by expressing phytoene dehydrogenase CarB, phytoene synthase / lycopene cyclase CarRP, β-carotene ketolase CrtW, β-carotene hydroxylase CrtZ, and ferricoxane reductase RFNR in a host strain. RFNR Derived from Chlamydomonas rhinelandii Chlamydomonas reinhardtii Haematococcus pluvialis Haematococcus pluvialis This strain, through heterologous expression of RFNR, efficiently provides reducing power to CrtZ, significantly enhancing the catalytic activity of CrtZ and ultimately achieving higher astaxanthin yield.

The invention discloses a non-genotype-limited watermelon genetic transformation method and application thereof, and relates to the technical field of plant genetic engineering. The method comprises the following steps: taking a cotyledon near-hypocotyl end of a watermelon cotyledon with the seedling age of 2-3 days as an explant, carrying out agrobacterium infection, co-culturing, recovering culture, bud induction and rooting culture, and thus obtaining a watermelon genetic transformation seedling. Based on the method, a CRISPR / Cas9 gene editing vector of a targeted watermelon phytoene dehydrogenase (PDS) gene is transformed, so that a watermelon transgenic positive material with a whitening phenotype is successfully obtained from a plurality of watermelon varieties. The invention overcomes the problems that the existing watermelon genetic transformation system is greatly limited by genotypes, is difficult to transform in common cultivated varieties and the like, establishes a non-genotype-limited watermelon genetic transformation system, and has important significance for creating excellent watermelon germplasm and promoting watermelon molecular breeding progress.

The invention discloses an Eucommia virus induced gene silencing system as well as a construction method and application thereof. The system is obtained by infecting Eucommia ulmoides with a target gene fragment recombinant virus plasmid carrying a target gene through agrobacterium tumefaciens; a target gene is an Eucommia ulmoides phytoene dehydrogenase gene, namely an Eucommia ulmoides EuPDS gene, and the nucleotide sequence of the EuPDS gene is as shown in SEQ ID NO. 1; the target gene segment is a conservative CDS sequence on the EuPDS gene of Eucommia ulmoides, and the nucleotide sequence of the target gene segment is as shown in SEQ ID NO.2. The Eucommia virus-induced gene silencing system can be used for studying Eucommia EuPDS gene functions, Eucommia phytoene dehydrogenase metabolic pathways or Eucommia stress resistance, and has profound significance for studying the Eucommia gene functions.

A herbicidal composition and a method for controlling weeds, including one or more uracil compounds of formula (I) and a compound of formula (II), and a herbicide compound of group B and a safener of group C. A weight ratio of the uracil compounds to the herbicide compound group B and the safener group C is 1:0.02 to 1:50, and the herbicide compound of group B is one or more of compounds B-1 to B-12: B-1, acetolactate synthase inhibitors; B-2, acetyl CoA carboxylase inhibitors; B-3, protoporphyrinogen IX oxidase inhibitors; B-4, 4-hydrophenylpyrubic acid dioxygenase inhibitors; B-5, phytoene desaturase inhibitors; B-6, photosystem II inhibitors; B-7, very-long-chain fatty acid synthesis inhibitors; B-8, microtubule formation inhibitors; B-9, auxin-type herbicides; B-10, enolpyruvylshikimate-3-phosphate synthase inhibitors; B-11, glutamine synthase inhibitors; and B-12, other herbicides; or agriculturally acceptable salts or derivatives thereof

The invention discloses establishment and optimization of a betula platyphylla high-efficiency gene editing system, which comprises methods of editing carrier screening, culture condition optimization and application thereof, and belongs to the technical field of plant gene engineering. Specifically, a betula platyphylla phytoene dehydrogenase gene (BpPDS) is taken as a target, three sgRNA targets are designed, the editing efficiency of AtU6, pEgP237 and pHSE-401 gene editing vectors in betula platyphylla is compared through an agrobacterium tumefaciens-mediated betula platyphylla genetic transformation method, and finally an applicable and efficient gene editing vector is screened out. The influence of high-temperature, low-temperature, drought and dark treatment on the betula platyphylla gene editing efficiency is further studied, and it is found that the betula platyphylla editing efficiency can be greatly improved by treating at 37 DEG C for 4 h and can be improved to 87.24% at most. Besides, the BpHAT4 gene of the betula platyphylla is edited by applying the BpHAT4 gene, a mutant is successfully obtained, and the drought resistance of the mutant is evaluated. Therefore, the established and optimized efficient gene editing system can efficiently edit the betula platyphylla target gene, and a convenient tool is provided for betula platyphylla gene function research and germplasm innovation.

The invention discloses a pinus massoniana gene silencing method, which is based on a plant virus vector and can effectively realize pinus massoniana gene silencing through an optimized transduction method. Experimental results based on a phytoene dehydrogenase gene (PmPDS) show that the silencing efficiency of the method can reach 79.1%; experimental results based on a plant geranyl geranyl pyrophosphate synthase 6 (PmGGPPS6) and an NAC transcription factor family 5 gene (PmNAC5) show that the method has wide applicability and can be used for silencing different types of genes. According to the method, the application range in the field of pinus massoniana gene function research is widened, possibility is provided for deep research of gene functions in multiple aspects such as pinus massoniana growth and development and metabolism regulation, and a new thought and method are provided for genetic improvement and variety cultivation of pinus massoniana; the cultivation of pinus massoniana varieties with specific phenotypes or excellent characters is facilitated, and the economic value and ecological benefits of the pinus massoniana varieties are improved.

The invention belongs to the field of plant genetic engineering, and relates to a reporter gene of subprostrate sophora, in particular to a phytoene dehydrogenase gene StPDS of subprostrate sophora and application of the phytoene dehydrogenase gene StPDS. The nucleotide sequence of the gene StPDS is as shown in SEQ ID No. 1. According to the invention, cloning and subcellular localization analysis are carried out on the StPDS gene in the chloroplast of subprostrate sophora for the first time; a subprostrate sophora StPDS gene silencing system is constructed, and the system has the characteristics of rapidness, high throughput, good effect and easiness in operation; according to the StPDS gene and the silence system, the expression level of the subprostrate sophora StPDS gene is effectively reduced, yellowing phenotype is caused, and the subprostrate sophora StPDS gene can be used as a reporter gene of a VIGS silence system in subprostrate sophora application. A solid theoretical and practical foundation is laid for application of the VIGS technology in the subprostrate sophora, and a basis is provided for verification and identification of other functional genes of the subprostrate sophora.

This invention provides a wild barley phytoene dehydrogenase gene. HbPDS Specific fragments and applications. A [structure / system] was constructed. HbPDS Gene-specific fragments, recombinant viral vector pTRV2-HbPDS, and the first establishment of wild barley HbPDS The VIGS gene silencing system can silence the wild barley phytoene dehydrogenase gene. HbPDS This inhibits the synthesis of carotenoids in wild barley, leading to phenomena such as chlorophyll fading and whitening of leaves.

The present invention relates to the field of genetic engineering technology, in particular to ground cucumber CtPDS Gene and VIGS virus vector and silencing method thereof. The present invention provides a phytoene dehydrogenase of cucurbita oleracea, the amino acid sequence of which is shown in SEQ ID NO.1. The present invention discovered the amino acid sequence of cucurbita oleracea phytoene dehydrogenase and its encoding gene CtPDS sequence, on this basis, provides a kind of silencing CtPDS Gene target nucleic acid molecule, VIGS silencing system and silencing method. CtPDS Leaves of gene-silenced plants CtPDS The gene expression level was significantly reduced, the silencing efficiency reached 56%~65%, the chlorophyll and carotenoid contents decreased significantly, and the leaves showed a whitening phenotype, providing technical support for the functional research of key genes in the ground melon.

The invention discloses a genetically engineered bacterium for producing phytoene, lycopene and beta-carotene and application of the genetically engineered bacterium, and belongs to the technical field of genetic engineering and metabolic engineering. According to the invention, endogenous phytoene synthase CrtB and phytoene dehydrogenase gene CrtI in rhodotorula host bacteria are knocked out, expression of 2-acylglycerol O-acyltransferase gene DGAT and fatty acid synthase gene FAS is knocked down, and finally, CrtB, CrtI and LCYB are selectively or jointly introduced into cytoplasm-endoplasmic reticulum-peroxisome to obtain the engineering bacteria. According to the engineering bacterium provided by the invention, glucose is used as a carbon source, the yield of phytoene, the yield of lycopene and the yield of beta-carotene are respectively and maximally 82.9 mg / L, 77.1 mg / L and 74.6 mg / L when the engineering bacterium is fermented in a 250 mL shake flask, and the cell dry basis content is more than or equal to 10%; the HPLC purity is greater than or equal to 95%.

The invention relates to the technical field of genetic engineering, and discloses a bacillus subtilis strain for producing natural products and a construction method thereof. Functional genes of a geranyl pyrophosphate synthase gene, a phytoene dehydrogenase gene and a phytoene synthetase gene for expressing and synthesizing lycopene are integrated in a chromosome of a starting bacillus subtilis strain, and one or more of 12 genes in total including 9 genes in an MEP (Methyl Ester Protein) pathway can be further integrated. The method comprises the following steps: constructing a recombinant strain of lycopene, endowing each gene with a to-be-edited RBS composed of eight independent continuous G basic groups to obtain a chassis bacterium, expressing dCas9-AID fusion protein and gRNA of a targeted RBS core region in the chassis bacterium to obtain a mutant library of which the expression levels of 12 genes are regulated at the same time, and performing high-throughput screening to obtain the recombinant strain of high-yield lycopene. According to the invention, a biological engineering strategy is adopted, and the constructed recombinant bacillus subtilis is used for synthesizing lycopene, so that a new idea is provided for high yield of lycopene by a biological method.

This invention relates to a genetically engineered strain for synthesizing retinal, its construction method, and its applications. The construction method involves introducing DNA fragments of the β-carotene 15,15'-oxygenase gene, isopentenyl pyrophosphate isomerase gene, phytoene dehydrogenase gene, geranyl-geranyl pyrophosphate synthase gene, and phytoene synthase gene into *Rhizopus cylindrica* to obtain a genetically engineered strain. This strain enhances the expression of β-carotene 15,15'-oxygenase, isopentenyl pyrophosphate isomerase, phytoene dehydrogenase, geranyl-geranyl pyrophosphate synthase, and phytoene synthase in *Rhizopus cylindrica*. By selecting *Rhizopus cylindrica* as a host, introducing key synthetic genes, and achieving stable genome integration, this method yields an engineered strain capable of efficiently synthesizing retinal using glucose as a carbon source, simplifying the production process, reducing costs, and improving industrial applicability.

The application discloses a kind of lycopene extracellular transport Saccharomyces cerevisiae strain construction and its application, belong to fermentation engineering technical field.The application constructs a kind of recombinant Saccharomyces cerevisiae strain of lycopene extracellular secretion ability, the recombinant Saccharomyces cerevisiae expressed truncated 3-hydroxy-3-methylglutaryl coenzyme A reductase, isopentenyl pyrophosphate isomerase, farnesyl pyrophosphate synthase, endoplasmic reticulum size regulator INO2, NADH kinase POS5 and ABC transporter Snq2;Heterologous expression of geranylgeranyl diphosphate synthase, phytoene dehydrogenase, bifunctional lycopene cyclase / octahydrolycopene synthase mutant CrtYBM1 (W61R) ;Knock out ROX1, EXG1 and GAL80 gene in Saccharomyces cerevisiae;And back-up orotic acid 5'-phosphorodecarboxylase expression;When its shake flask fermentation, the lycopene content of this strain extracellular reaches 12.58mg / L, and is increased to 16.5 times of starting strain, thus has wide application prospect.

The invention belongs to the technical field of plants, and particularly relates to a coix phytoene dehydrogenase ClPDS gene and a construction method of a VIGS silencing system. The invention provides a coix phytoene dehydrogenase ClPDS gene. The nucleotide sequence of the coix phytoene dehydrogenase ClPDS gene is as shown in SEQ ID No. 1; silencing a specific fragment of the coix phytoene dehydrogenase ClPDS gene, wherein the specific fragment is a fragment I, a fragment II or a fragment III; the nucleotide sequences of the fragment I, the fragment II and the fragment III are respectively shown as SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 4. After the specific fragment is silenced, carotenoid conversion is inhibited, chlorophyll loses protection of carotenoid, chlorophyll can be destroyed by strong light for green removal, and finally, an obvious photobleaching phenomenon appears at a silenced part in a phenotype.