34 results about "Diuraphis noxia" patented technology

The invention relates to the field of plant protection, and provides a simple wheat aphid species assay method. The method includes A, obtaining wheat seedlings with complete roots and leaves through water planting; B, fetching a bunch of the wheat seedlings, placing the roots into an centrifugal tube, sealing the mouth of the centrifugal tube after wrapping the stem base portion through moist absorbent cotton, retaining the leaves outside the centrifugal tube, and obtaining the wheat seedlings with the centrifugal tube; C, moisturizing a culture dish provided with filter paper by spraying, and placing the wheat seedlings with the centrifugal tube; D, planting wheat aphids on the leaves of the wheat seedlings with the centrifugal tube, processing according to experiment design, and recording assay results. According to the method, during biological assay, the water-planting wheat seedlings with the roots are placed into the centrifugal tube ingeniously, the wheat seedlings can be retained in vivo during the entire experiment, and the accuracy of biological activity assay is improved greatly; according to the method, only the culture dish, the centrifugal tube and the absorbent cotton are required, cost is low, operation is simple, practicality is high, and biological activity of the object to be assayed on natural conditions can be shown better.

The invention discloses a dsRNA (double-stranded ribonucleic acid) for inhibiting expression of wheat aphid cytochrome C oxidase VIIc subunit gene and application thereof. The invention provides a dsRNA molecule disclosed as Sequence 2 in the sequence table. The invention also provides a DNA (deoxyribonucleic acid) molecule for coding the RNA molecule. The invention also provides application of the RNA molecule or the DNA molecule, which is at least one of the following (1) to (4): (1) preventing and treating aphids; (2) promoting death of aphids; (3) inhibiting growth of aphids; and (4) inhibiting expression of the aphid in-vivo cytochrome C oxidase VIIc subunit gene. The dsRNA has important application value in preventing and treating aphids in agricultural production.

The invention discloses a method for cultivating dryland wheat simply at high yield and high efficiency. The method solves the problems that existing intensive cultivation is high in cost, low and unstable in yield and unsuitable for mechanical cultivation. The method comprises the steps that a, when the wheat is harvested mechanically, high stubble is reserved and returned to the field completely; b, deep ploughing is conducted, and diammonium phosphate fertilizer containing phosphorus pentoxide is applied to the field; c, weeding is conducted after the wheat is harvested and before deep ploughing is conducted; d, coated seeds are selected and used for seeding; e, seeding is conducted when the daily average temperature ranges from 15.5 DEG C to 18.0 DEG C, and the seeding rate ranges from 8.0 kg to 10.0 kg / mu; f, before rotary tillage seeding repression, urea is applied to the field for 16.5-18.5 kg / mu, the rotary tillage depth ranges from 14 cm to 16 cm, the seeding depth ranges from 4 cm to 5 cm, and ridges are made flat through a rake after seeding; g, after seedlings of the wheat emerge, chemical weeding is conducted in time; h, if the soil moisture content is large, ploughing is conducted when the earth surface begins unfreezing, soil is crashed and the field is flattened after ploughing, and soil moisture is preserved ; i, red spiders and wheat aphids are prevented and controlled by means of spraying mist; j, one spraying and three prevention is conducted according to the conditions of diseases and insect pests, and spraying is conducted once again every 10 d; k, in the last dough stage of the wheat, the wheat is harvested mechanically in time. By means of the method, the moisture storage capacity of the soil with the thickness ranging from 0 to 200 cm is increased apparently, and the utilization rate of nitrogenous fertilizer and the utilization rate of phosphate fertilizer in current seasons are increased by 3.58%-5.69% and 1.86%-3.93% respectively.

The invention relates to a chemically-defined diet and artificial mass rearing method for wheat aphids. The chemically-defined diet for the wheat aphids comprises an artificial diet applicable to the growth and development of Sitobion avenae and an artificial diet applicable to the proliferation of Sitobion avenae, is an optimized formula of the two kinds of diets and can enable Sitobion avenae to survive normally, grow, develop and reproduce offspring, thereby having a broad application prospect. The rearing method for Sitobion avenae, provided by the invention, is characterized in that the growth and development diet is adopted at a young aphid stage of Sitobion avenae; and the proliferation and development diet is adopted at an adult aphid stage of Sitobion avenae. According to the chemically-defined diet and the artificial mass rearing method, the adult ratio and birth ratio of the aphids are increased, the mass rearing method is established, and a reliable and important evaluation means for providing test aphids with uniform nutrition background and implementing researches on aphid nutrition physiology, wheat insect-resisting substances and transgenic insect-resisting protein activity is provided.

The invention discloses dsRNA of a gene of a symbiotic bacterium of aphids and an application thereof to inhibition of growth of aphids. DsRNA provided by the invention is double-stranded RNA formed by a nucleotide shown in a sequence 2 in a sequence table and a nucleotide shown in an inverse complementary sequence of the sequence 2. Experiments prove that the gene of the endotrophic symbiotic bacterium hamiltonella defensa 28469 of wheat aphids is obtained; the gene of the endotrophic symbiotic bacterium hamiltonella defensa 28469 of wheat aphids can be inhibited or silenced, impaired growth and development of the wheat aphids can be caused and lethal effects can be generated by adopting the method of feeding dsRNA in vitro.

The invention discloses a wheat aphid quantity forecasting method and system based on human being evolution gene expression programming. The method is characterized in that the rouging operation is adopted to repair an inferior individual or infeasible individual generated in the natural evolution and to convert the inferior individual or infeasible individual into a feasible individual, the optimizing operation is adopted to enable high-quality gene fragments in a high-quality gene pool to be dispersed in a population, so that the quality of the individual in the population can be effectively improved, and the rate of convergence of the algorithm can be accelerated; the population intervention is adopted to solve the premature convergence phenomenon caused by the loss of diversity of the population in the natural evolution process, the novel feasible individual which is randomly generated in the population and a mirror individual which is generated in a mirror mapping manner and is large in difference are used for substituting the poor individual of the same quantity, so that the diversity of the population is increased, and the global optimizing capacity of the algorithm can be effectively improved.

The present invention discloses a wheat seed treatment agent composition and belongs to the technical field of pesticides. The wheat seed treatment agent compound comprises the active ingredients by weight percentage based on the composition: 1%-60% of imidacloprid or thiamethoxam, 1%-25% of avermectin or emamectin benzoate and 0.5%-25% fipronil. The wheat seed treatment agent composition disclosed by the invention is prepared from the three active ingredients, all of which have synergic effects compared with each of the three single dosages, and particularly has a remarkable synergic effect on prevention and treatment of wireworms. According to the wheat seed treatment agent composition, the amount of pesticides used in field can be greatly reduced, the insecticidal spectrum can be expanded, and the validity period is long. Main pests such as wheat aphid, heterodera avenae wollenweber and wireworms and the like in the growth period of the wheat through one-time seed dressing can be prevented and treated. The composition has characteristics of safety, high efficiency, low toxicity and labor saving, and can make a greater contribution to the production increment of wheat.

The invention discloses dsRNA (double-stranded Ribose Nucleic Acid) for inhibiting expression of a salivary glandular secretion polypeptide gene of wheat aphids and application thereof. The invention provides a dsRNA molecule which is as shown in a sequence 2 in a sequence table. The invention also protects a DNA (Deoxyribose Nucleic Acid) molecule for encoding the RNA molecule. The invention also protects application of the RNA molecule or the DNA molecule. The application comprises at least one of the following application: (1) preventing and treating aphids; (2) promoting death of the aphids; (3) inhibiting the aphids from growing; (4) inhibiting expression of the in-vivo salivary glandular secretion polypeptide gene of the aphids. The dsRNA and the application thereof have important application value for preventing and treating the aphids in the agricultural production.

The invention discloses a dsRNA (double-stranded ribonucleic acid) for inhibiting expression of wheat aphid serine proteinase I gene and application thereof. The invention provides a dsRNA molecule disclosed as Sequence 2 in the sequence table. The invention also provides a DNA (deoxyribonucleic acid) molecule for coding the RNA molecule. The invention also provides application of the RNA molecule or the DNA molecule, which is at least one of the following (1) to (4): (1) preventing and treating aphids; (2) promoting death of aphids; (3) inhibiting growth of aphids; and (4) inhibiting expression of the aphid in-vivo serine proteinase I gene. The dsRNA has important application value in preventing and treating aphids in agricultural production.

The invention discloses a synergistic pesticide composition containing triflumezopyrim, and applications thereof, and belongs to the technical field of pesticide. The synergistic pesticide compositioncontaining triflumezopyrim contains active components A and B; the active component A is triflumezopyrim; and the active component is one selected from plant source pesticide matrine, nimbin, and pyrethrin. The effect mechanisms of the active component A and the active component B of the synergistic pesticide composition are completely different; combination of the two active components is capable of realizing synergistic effect on pests, improving prevention effect, reducing pesticide using amount, and reducing drug resistance; and the synergistic pesticide composition can be used for prevention and control of insects with piercing-sucking mouthparts such as wheat aphid, cotton aphid, corn aphid, bean aphid, potato aphid, apple aphid, tomato whitefly, gray planthopper, brown planthopper,false eye green leafhopper.

The invention discloses a trapping composition for hoverflies and application thereof in the technical field of the preparation of insect sex pheromone. The trapping composition contains an insect pheromone and a plant volatile matter, wherein the insect pheromone is cis-11-hexadecene acetate, the plant volatile matter is one or more of ocimene, nerol and alpha-caryophyllene, and the weight ratioof the insect pheromone to the plant volatile matter is (50-100) to 1. The trapping composition provided by the invention is easy to prepare, and raw materials are easily available; the composition has very strong trapping capacity, good stability and long lasting period, and the prevention and treatment effects last for 30 days or above; the specifity is strong, and the resistance of the hoverflies cannot be generated; the trapping composition is harmless to human and livestock and environment-friendly; and the trapping composition is strong in stress resistance and convenient to use. The trapping composition can be used for controlling pest situations of hoverflies and trapping a large amount of the hoverflies to capture cereal aphids and has very important significance to the comprehensive treatment of the cereal aphids.

The invention belongs to the technical field of wheat aphid physiology and phytopathology, and particularly relates to a piercing-sucking type insect thin film virus feeding method and application thereof. The method comprises the following steps: (1) uniformly thinning a plastic film, and covering the upper end of a container with openings at the two ends with the plastic film; (2) dropwise adding a virus feeding solution on the thin film; (3) uniformly thinning the plastic film, and covering the virus feeding solution with the plastic film; (4) inclining the opening at the lower end of the container upwards, and putting the insects into the container through the opening at the lower end; and (5) putting the container filled with the insects onto absorbent paper with the lower end opening facing downwards, and putting the container onto a plane for virus feeding. The method is simple and convenient to operate, effectively improves the efficiency of sucking food or virus solution by the insects, and provides convenience for further research on the virus transmission efficiency between the insects and plants.

The invention relates to the field of plant protection, and provides a simple wheat aphid species assay method. The method includes A, obtaining wheat seedlings with complete roots and leaves through water planting; B, fetching a bunch of the wheat seedlings, placing the roots into an centrifugal tube, sealing the mouth of the centrifugal tube after wrapping the stem base portion through moist absorbent cotton, retaining the leaves outside the centrifugal tube, and obtaining the wheat seedlings with the centrifugal tube; C, moisturizing a culture dish provided with filter paper by spraying, and placing the wheat seedlings with the centrifugal tube; D, planting wheat aphids on the leaves of the wheat seedlings with the centrifugal tube, processing according to experiment design, and recording assay results. According to the method, during biological assay, the water-planting wheat seedlings with the roots are placed into the centrifugal tube ingeniously, the wheat seedlings can be retained in vivo during the entire experiment, and the accuracy of biological activity assay is improved greatly; according to the method, only the culture dish, the centrifugal tube and the absorbent cotton are required, cost is low, operation is simple, practicality is high, and biological activity of the object to be assayed on natural conditions can be shown better.

The invention belongs to the technical field of wheat cultivation, and particularly relates to a wheat cultivation method. According to the wheat cultivation method, in the wheat cultivation process, when aphids appear on wheat plants, a synergistic insecticide is sprayed, and the synergistic insecticide is prepared in the mode that propylene glycol alginate and chromafenozide or flupyradifurone are subjected to binary compounding to serve as an active ingredient, and auxiliary agents acceptable in pesticide are supplemented. According to the wheat cultivation method disclosed by the invention, the action mechanisms and action sites of the effective components propylene glycol alginate and chromafenozide or flupyradifurone of the sprayed synergistic insecticide are different, and multiple sites act together when pests are killed, so that the generation of the drug resistance of wheat aphids can be effectively delayed, and the updating period of the insecticide is prolonged to a certain extent; meanwhile, a synergistic effect is shown during compounding, the prevention and treatment effect on wheat aphids can be improved, the application dosage of the pesticide can be reduced, then environmental pollution and pesticide residues are reduced, and the prevention and treatment cost of diseases and insect pests in wheat cultivation is reduced.

The invention discloses an agricultural pesticide. The active components are afidopyropen and pymetrozine at a mass ratio of 1:80-80:1. The composition possesses excellent synergistic effect, the insecticidal activity is better than that of single components, and excellent prevention and controlling effect on a plurality of pests such as rice planthopper, powdery mildew, corn borer, cotton bollworm, wheat rust, cotton aphid, rice sheath blight, rice blast, wheat bran, red spider, and aphid is achieved.

The invention discloses a flower and plant composition for increasing natural enemies to control pests in rice and wheat rotation plough land and application. The flower and plant composition covers 12 herbs in 8 families, the plant type number is moderate, management and protection are simple, and the plant form structure and ecological functions are matched scientifically and reasonably. The flower and plant composition is used for constructing an artificial plant buffer belt in the rice and wheat rotation plough land, firstly, diffusion of malignant weeds can be inhibited, inhabit and reproduction places in a rice and wheat rotation plough system for small animals and arthropods can be optimized, and the farmland biodiversity (species and abundance) can be increased; secondly, the artificial plant buffer belt can cover multiple natural enemies and continuously serve as a source place of natural enemies for rice and wheat growth seasons, besides, rice borers and wheat aphids can be attracted to the flower and plant belt to be killed by natural enemies, the continuous and stable control effect on rice borer and wheat aphid populations is realized, the dependence of intensive paddyand dry fields on chemical pesticides is significantly reduced, so that green, efficient and sustainable development of agriculture and quality safety of agricultural products are supported, and theesthetic effect of rural farmland landscape is enhanced.

The invention discloses a broad-spectrum botanical insecticide and a preparation method thereof, belonging to the technical field of agricultural pest control and prevention. 1 to 3 parts of ragweed; the broad-spectrum botanical insecticide provided by the present invention uses invasive plants as raw materials, reuses harmful organisms, has the advantage of green safety, and is effective against wheat aphid, Spodoptera litura, The maximum control effects of corn borer and corn aphid in 72 hours were 95.7%, 97.4%, 94.2% and 96.9%, respectively, which had significant insecticidal effects.

The invention discloses a dsRNA (double-stranded ribonucleic acid) for inhibiting expression of wheat aphid cytochrome C oxidase VIIc subunit gene and application thereof. The invention provides a dsRNA molecule disclosed as Sequence 2 in the sequence table. The invention also provides a DNA (deoxyribonucleic acid) molecule for coding the RNA molecule. The invention also provides application of the RNA molecule or the DNA molecule, which is at least one of the following (1) to (4): (1) preventing and treating aphids; (2) promoting death of aphids; (3) inhibiting growth of aphids; and (4) inhibiting expression of the aphid in-vivo cytochrome C oxidase VIIc subunit gene. The dsRNA has important application value in preventing and treating aphids in agricultural production.

The invention discloses a wheat growth period field aphid resistance screening and identifying device. The device comprises a structural frame, an isolation hood and a zipper, wherein the isolation hood is arranged outside the structural frame, supported by the structural frame to form a relatively isolated wheat growth space, ventilated and pervious to light, and the zipper is arranged at the side portion of the isolation hood and used for controlling the isolation hood to be sealed and opened. The radius of the upper end and the radius of the lower end of the isolation hood are the same, thewhole isolation hood is cylindrical, the structural frame comprises a plurality of supporting rods with the same length, the device is placed in a field for conventional management, wheat and aphidsare tested in a relative space, and the number of wheat plants and the number and variety of wheat aphids can be considered to be operated as needed. The wheat aphids are fed specially and cannot be affected by external factors, results such as the aphid quantity ratio obtained through statistics are accurate, real, stable and reliable, and the wheat aphid resistance level can be truly reflected.

The invention discloses a method for synergistic biological control of wheat aphids and aphids based on Aphids tabaciformis, comprising the following steps: S1: before planting and sowing wheat, plowing the wheat field, removing weeds, sowing wheat, And when the wheat is sown, apply agricultural compound fertilizer; water and fertilize the wheat seedlings at the right time to ensure the normal growth of the wheat seedlings; Aphid seedlings are collected, and the substrate of the tobacco seedlings has a good water retention effect, and can survive in the wheat field for more than 10 days, which can ensure that all the aphids on the tobacco seedlings have eclosion into adults; the present invention, through It is expected that the wheat aphids will be released before the peak period of occurrence of wheat aphids to effectively control the growth of the number of wheat aphids. It can make better use of the wheat aphids to reproduce the cocoon wasps, prevent and control the wingless aphids in the tobacco field, and realize the prevention and control of wheat aphids and tobacco aphids. Synergistic biological control.

The invention belongs to the technical field of aphid feeding, and provides an indoor aphid feeding method to solve the problems that in an existing aphid feeding device and method, the survival rate of contemporary and secondary aphids is low, and the device is difficult to operate. Fresh crop leaves are inserted into an aphid feeding device and are used for feeding aphids, the device comprises a cylindrical transparent feeding pipe with the two ends opened, a plant growing block is arranged at one end of the feeding pipe, a sponge seal layer is arranged at the other end, a nutrition layer is arranged in the plant growing block, a plant inserting opening is formed in the center of the top, and crop leaves are inserted into the plant inserting opening. The fresh and tender degree and growth of the plant leaves are ensured, the farrowing rate is increased, the death rate is reduced, the population doubling time is shortened, and the net appreciation rate of population is increased. It is ensured that the growth environment is relatively independent, and aphids are prevented from escaping or dying abnormally. The feeding device is transparent and small, the aphids are easy to find through the feeding device, aphid growth and propagation conditions are convenient to observe and record, the number of times of operation is reduced, the feeding device is durable, breakage-proof, reusable and low in manufacturing cost and facilitates carrying and one-handed operation.

The invention provides a dibenzocaprolactam pyrazolidine compound and a preparation method thereof. The preparation method of the compound includes the following steps of: reacting an iodo-biphenyldicarboxaldehyde compound with a hydrazide compound in an alcohol solvent to obtain an acylhydrazone compound, then substituting iodine in the acylhydrazone compound by a double bond, and then carrying out [3+2] cyclization reaction on the double bond and acylhydrazone to obtain the dibenzocaprolactam pyrazolidine compound. The dibenzocaprolactam pyrazolidine compound disclosed by the invention has certain insecticidal activity, particularly has good insecticidal activity on wheat aphids and whiteflies, also has certain insecticidal activity on cotton bollworms and red spiders, and can be appliedto preparation of new pesticides; the dibenzocaprolactam pyrazolidine compound disclosed by the invention has the advantages of good substrate universality and high reaction yield, is low in toxicityto normal cells of a human body, and has good applicability.