34 results about "Diuraphis noxia" patented technology

The invention discloses dsRNA (double-stranded Ribose Nucleic Acid) for inhibiting expression of a salivary glandular secretion polypeptide gene of wheat aphids and application thereof. The invention provides a dsRNA molecule which is as shown in a sequence 2 in a sequence table. The invention also protects a DNA (Deoxyribose Nucleic Acid) molecule for encoding the RNA molecule. The invention also protects application of the RNA molecule or the DNA molecule. The application comprises at least one of the following application: (1) preventing and treating aphids; (2) promoting death of the aphids; (3) inhibiting the aphids from growing; (4) inhibiting expression of the in-vivo salivary glandular secretion polypeptide gene of the aphids. The dsRNA and the application thereof have important application value for preventing and treating the aphids in the agricultural production.

The invention discloses a method and system for predicting the number of wheat aphids based on human evolutionary gene expression programming. The "removing inferior" operation is used to repair inferior or infeasible individuals generated in natural evolution and transform them into viable individuals. The "enhancing optimization" operation is to make the high-quality gene fragments in the high-quality gene bank diffuse in the population, thereby effectively improving the quality of individuals in the population and speeding up the convergence speed of the algorithm; using population intervention, due to population diversity in the natural evolution process, due to population diversity The phenomenon of premature convergence caused by deletion is to replace the same number of poor individuals with new randomly generated feasible individuals and mirror individuals with a large degree of difference generated by mirror mapping in the population to increase the diversity of the population, thereby effectively improving. The global optimization ability of the algorithm.

The invention discloses a trifluoropyrim-containing synergistic insecticide composition and an application thereof, belonging to the technical field of pesticides. The insecticide composition of the present invention comprises two active components A and B, wherein the active component A is trifluoropyrim, and the active component B is one of the botanical pesticides matrine, azadirachtin and pyrethrin. In the insecticide composition of the present invention, the action mechanism of the active ingredient A and the active ingredient B are completely different, and the combination can synergize the pests, improve the control effect, reduce the amount of pesticide use, delay the generation of drug resistance, and reduce the risk of drug resistance. It can be used for the control of piercing-sucking mouthparts such as wheat aphid, cotton aphid, corn aphid, bean aphid, potato aphid, apple cotton aphid, tomato whitefly, brown planthopper, brown planthopper, and green leafhopper.

The invention discloses a slow-release nano-pesticide and a preparation method thereof, relating to the field of biological pesticides, which is a slow-release preparation of nano-scale (azadirachtin / chitosan) synthesized from nano-chitosan and azadirachtin, It has a three-dimensional network conformation and slow-release characteristics; the preparation is completely soluble in water and has good stability, and its indoor toxic activity against crop aphid pests such as tobacco aphid, cabbage aphid, wheat aphid and orange aphid has increased by 54.47% to 98.41%, The indoor virulence activity of Lepidoptera pests such as tobacco budworm, cabbage caterpillar, diamondback moth and Spodoptera litura has increased by 31.75% to 65.35%, and the insecticide provided by the invention is used for field control. Crop aphid pests such as wheat aphid and orange aphid, and Lepidoptera pests such as tobacco budworm, cabbage caterpillar, diamondback moth and Spodoptera litura have good insecticidal effects.

The invention relates to the field of plant protection, and provides a simple wheat aphid species assay method. The method includes A, obtaining wheat seedlings with complete roots and leaves through water planting; B, fetching a bunch of the wheat seedlings, placing the roots into an centrifugal tube, sealing the mouth of the centrifugal tube after wrapping the stem base portion through moist absorbent cotton, retaining the leaves outside the centrifugal tube, and obtaining the wheat seedlings with the centrifugal tube; C, moisturizing a culture dish provided with filter paper by spraying, and placing the wheat seedlings with the centrifugal tube; D, planting wheat aphids on the leaves of the wheat seedlings with the centrifugal tube, processing according to experiment design, and recording assay results. According to the method, during biological assay, the water-planting wheat seedlings with the roots are placed into the centrifugal tube ingeniously, the wheat seedlings can be retained in vivo during the entire experiment, and the accuracy of biological activity assay is improved greatly; according to the method, only the culture dish, the centrifugal tube and the absorbent cotton are required, cost is low, operation is simple, practicality is high, and biological activity of the object to be assayed on natural conditions can be shown better.

The invention discloses dsRNA (double-strand ribose nucleic acid) capable of inhibiting wheat aphid salivary protein MYS2 gene expression and application thereof. The invention provides the double-stranded RNA molecule shown in the sequence 2 in a sequence table. The DNA (deoxyribonucleic acid) molecule encoding the RNA molecule and the application of the RNA molecule or DNA molecule are protected in the invention, and the application of RNA molecule or DNA molecule includes at least one of the following: (1) preventing and treating aphid; (2) promoting death of aphid; (3) inhibiting growth of aphid; and (4) inhibiting expression of in vivo salivary protein MYS2 gene of aphid. The dsRNA has important application value in prevention and control of aphid in agriculture production.

An intercropping planting method with both aphid control and soil heavy-metal control functions, including the following steps: in a first year, winter wheat and wintering herbage are intercropped, wherein the wintering herbage is a heavy-metal enriched plant or a heavy metal super-enriched plant; in early spring of a second year, larvae of ladybugs loving to eat aphids and aphids loving to eat the herbage are cultivated indoors; when the temperature rises to a degree capable of ensuring the survival of the ladybugs and the aphids, the ladybugs and the aphids are released to the herbage; then,wheat aphids loving eat wheat in a field are increased through natural breeding and the ladybugs eat the wheat aphids, when the number of the wheat aphids reaches a control index, the herbage is cutoff, thus the ladybugs are driven to completely transported to the winter wheat for eating the wheat aphids, and the heavy metal enriched plants are interplanted in the field where the herbage is cutoff; after the winter wheat is mature and aged, the ladybugs migrate to the heavy metal enriched plant; and after the winter wheat is harvested, summer corn or summer soybean is planted. The method can realize aphid biological control, heavy metal control, production of winter wheat, summer corn and the like, and intercropping production of economic crops.

The invention discloses an application of dsRNA of an ecdysone receptor (EcR) related gene to control of damage caused by aphids. DsRNA provided by the invention is double-stranded RNA formed by a nucleotide shown in a sequence 2 in a sequence table and a nucleotide shown in an inverse complementary sequence of the sequence 2. Experiments prove that dsRNA of cDNA of the EcR related gene of sitobion avenae is obtained; expression of the EcR gene in sitobion avenae can be inhibited, impaired growth and development of wheat aphids can be caused and lethal effects can be generated by adopting the method of feeding dsRNA in vitro.

The invention discloses a dsRNA (double-stranded ribonucleic acid) for inhibiting expression of wheat aphid cytochrome C oxidase VIIc subunit gene and application thereof. The invention provides a dsRNA molecule disclosed as Sequence 2 in the sequence table. The invention also provides a DNA (deoxyribonucleic acid) molecule for coding the RNA molecule. The invention also provides application of the RNA molecule or the DNA molecule, which is at least one of the following (1) to (4): (1) preventing and treating aphids; (2) promoting death of aphids; (3) inhibiting growth of aphids; and (4) inhibiting expression of the aphid in-vivo cytochrome C oxidase VIIc subunit gene. The dsRNA has important application value in preventing and treating aphids in agricultural production.

The invention discloses dsRNA of a gene of a symbiotic bacterium of aphids and an application thereof to inhibition of growth of aphids. DsRNA provided by the invention is double-stranded RNA formed by a nucleotide shown in a sequence 2 in a sequence table and a nucleotide shown in an inverse complementary sequence of the sequence 2. Experiments prove that the gene of the endotrophic symbiotic bacterium hamiltonella defensa 28469 of wheat aphids is obtained; the gene of the endotrophic symbiotic bacterium hamiltonella defensa 28469 of wheat aphids can be inhibited or silenced, impaired growth and development of the wheat aphids can be caused and lethal effects can be generated by adopting the method of feeding dsRNA in vitro.

The invention belongs to the technical field of aphid feeding, and provides an indoor aphid feeding method to solve the problems that in an existing aphid feeding device and method, the survival rate of contemporary and secondary aphids is low, and the device is difficult to operate. Fresh crop leaves are inserted into an aphid feeding device and are used for feeding aphids, the device comprises a cylindrical transparent feeding pipe with the two ends opened, a plant growing block is arranged at one end of the feeding pipe, a sponge seal layer is arranged at the other end, a nutrition layer is arranged in the plant growing block, a plant inserting opening is formed in the center of the top, and crop leaves are inserted into the plant inserting opening. The fresh and tender degree and growth of the plant leaves are ensured, the farrowing rate is increased, the death rate is reduced, the population doubling time is shortened, and the net appreciation rate of population is increased. It is ensured that the growth environment is relatively independent, and aphids are prevented from escaping or dying abnormally. The feeding device is transparent and small, the aphids are easy to find through the feeding device, aphid growth and propagation conditions are convenient to observe and record, the number of times of operation is reduced, the feeding device is durable, breakage-proof, reusable and low in manufacturing cost and facilitates carrying and one-handed operation.