A Simple Bioassay Method for Aphids
A bioassay and wheat aphid technology, applied in botany equipment and methods, soilless cultivation, cultivation, etc., can solve problems such as death, nutritional insecurity, and wheat aphid stylet damage, and achieve improved accuracy and strong practicability , the effect of simple operation
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Embodiment 1
[0032] Example 1 Determination of life parameters in the early reproductive period of Pipe aphid
[0033] Using a 160mm glass petri dish, spread the wheat seeds evenly on the bottom of the petri dish. Soak the seeds in clean water for 24 hours to promote rooting and germination of the seeds. After 24 hours, pour out the water in the petri dish, cover the seeds with two layers of white gauze soaked with water, wash the germinated seeds once a day with the gauze; wait until the plant grows to the height of the plant (from the base of the stem to the height of the tip) 30mm Remove the gauze around the time and continue to rinse the plants once a day to moisturize the roots of the plants and prevent mildew of the seeds.
[0034] When the plant grows to a plant height of about 60mm, pull up the whole wheat seedlings (be careful not to damage the roots), take 4-6 wheat seedlings and put their rhizomes into a 2ml centrifuge tube; soak the absorbent cotton with clean water, so as not to d...
Embodiment 2
[0041] Implementation 2 Bioassay of Alkaloid Insecticides on Pipe Aphid
[0042] Use a glass petri dish with a diameter of 160 mm to spread the wheat seeds evenly on the bottom of the petri dish. Soak the seeds in clean water for 24 hours to promote rooting and germination of the seeds. After 24 hours, drain the water in the petri dish, cover the seeds with two layers of soaked white gauze, wash the germinated seeds once a day with the gauze; when the plants grow to about 25mm, remove the gauze and continue to rinse the plants once a day. The roots of the plants are moisturizing and prevent seed mildew.
[0043] When the plant grows to about 80mm, pull up the whole wheat seedling (be careful not to damage the roots), take 3 to 4 wheat seedlings, and put their rhizomes into a 1.5ml centrifuge tube; soak the absorbent cotton with clean water, so as not to drip It is advisable to wrap the stem base with moist absorbent cotton and plug the opening of the centrifuge tube, keep the lea...
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