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Lactic acid bacteria formula and fermentation technique

A lactic acid bacteria and formula technology, applied in the direction of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of short shelf life, effect of storage time, inconvenient drinking of solid lactic acid bacteria, etc.

Inactive Publication Date: 2018-08-31
江苏恒康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the application effect of lactic acid bacteria is affected by many factors, such as formula type, processing technology, bacterial composition of the preparation, dosage, moisture, temperature, storage time, etc. will all have an impact on its effect
[0003] At present, lactic acid bacteria are divided into solid lactic acid bacteria and liquid level lactic acid bacteria. Among them, solid lactic acid bacteria are inconvenient to drink and need to be activated to play a role; liquid level lactic acid bacteria need to be transported and stored at low temperature, and have a short shelf life. They also need to be activated to play a role. Defects

Method used

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  • Lactic acid bacteria formula and fermentation technique

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Fermentation substrate treatment: crush 70kg of barley, add 250L of hot water at 40°C, stir well, add 10ml of glucoamylase, keep warm for 30min, then quickly heat up to 80°C within 20min, keep warm for 90min, and then pass 100 Take 200L supernatant after filtering.

[0025] (2) Preparation of fermentation broth: add 40kg isomalt, 2.5kg xanthan gum, 1kg pectin, 5kg sodium citrate, 100g sucralose, 100g potassium sorbate into 700L water, keep the temperature at 122°C, After autoclaving for 25 minutes, the initial fermentation liquid was obtained; then 100 g of vitamin C was added to 10 L of purified water, filtered through a pore size of 0.22 μm, and then added to the above initial fermentation liquid when the temperature was lowered to 40 ° C to obtain a fermentation liquid.

[0026] (3) Inoculation: After adding the fermented substrate treated in step (1) to the fermented liquid obtained in step (2), when the temperature is lowered to 36°C, inoculate 1000 g of Lactob...

Embodiment 2

[0029] (1) Fermentation substrate treatment: crush 35kg of barley and 20kg of rice, add 250L of hot water at 40°C, stir evenly, add 10ml of glucoamylase, keep warm for 90min, then quickly raise the temperature to 79°C within 25min, and keep warm for 80min , and then take 200L of the supernatant after filtering through 100 meshes to obtain a processed fermentation substrate.

[0030] (2) Preparation of fermentation broth: add 10kg isomalt, 2.5kg sodium carboxymethylcellulose, 1kg gellan gum, 3kg sodium citrate, 2kg sodium malate, 200g sucralose, and 100g potassium sorbate 700L of water, 121°C, 20min autoclaving to obtain the initial fermentation liquid; then add 100g of vitamin C to 9L and filter through a pore size of 0.22um, and then add it to the above-mentioned initial fermentation liquid when the temperature is lowered to 38°C to obtain the fermentation liquid.

[0031] (3) Inoculation: After adding the fermented substrate treated in step (1) to the fermented liquid obtain...

Embodiment 3

[0034] (1) Fermentation substrate treatment: crush 5kg of barley flour, 25kg of corn, and 25kg of Chinese yam, add 250L of hot water at 40°C, stir evenly, add 20ml of glucoamylase, keep warm for 70min, and then rapidly heat up to 75°C in 10min , keep warm for 90min, then take 200L of supernatant after filtering through 100 mesh, and obtain the processed fermentation substrate.

[0035] (2) Preparation of fermentation broth: add 15kg isomalt, 200g aspartame, 2.5kg sodium carboxymethylcellulose, 1kg gellan gum, 2kg sodium malate, 1kg sodium lactate, 100g potassium sorbate to 700L In water, keep the temperature at 123°C, autoclave for 30 minutes to obtain the initial fermentation liquid; then add 100g of vitamin C to 10 purified water and filter through 0.22um pore size, and when the antioxidant aqueous solution cools down to 37°C, add the above initial fermentation liquid , to obtain a fermentation broth.

[0036] (3) Inoculation: After adding the fermented substrate treated in...

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Abstract

The invention discloses a lactic acid bacteria formula. The formula comprises the following components in parts by weight of 10-20 parts of fermentation substrates, 0.2-0.5 part of a stabilizing agent, 0.01-0.02 part of a sweetening agent, 0.001-1 part of an acidity adjusting agent, 0.001-0.5 part of edible essence, 0.001-0.5 part of an antioxidant, 0.001-0.5 part of a preservative, 0.0001-0.001 part of lactic acid bacteria and 75-86 parts of water. Through adoption of the technique disclosed by the invention, complex carbohydrate parts can be converted into simple carbohydrate which can be directly utilized by the lactic acid bacteria, and the lactic acid bacteria can quickly grow at an early stage; and through the mutual competition mechanism of strains, remaining complex carbohydrate can slowly release, and the condition that bacterial strains can keep the living state at room temperature and for a long time can be guaranteed.

Description

technical field [0001] The invention relates to the field of food, in particular to a lactic acid bacteria formula and fermentation technology. Background technique [0002] As one of the substitutes of antibiotics, lactic acid bacteria are widely used in the field of food additives. In the prior art, the application effect of lactic acid bacteria is affected by many factors, such as formula type, processing technology, bacterial composition of the preparation, dosage, moisture, temperature, storage time, etc. will all have an impact on its effect. [0003] At present, lactic acid bacteria are divided into solid lactic acid bacteria and liquid level lactic acid bacteria. Among them, solid lactic acid bacteria are inconvenient to drink and need to be activated to play a role; liquid level lactic acid bacteria need to be transported and stored at low temperature, and have a short shelf life. They also need to be activated to play a role. Defects. Therefore, there is an urgen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L29/00C12N1/20C12R1/25C12R1/245C12R1/23C12R1/225C12R1/46C12R1/01
CPCA23L29/00C12N1/20
Inventor 顾玉琪
Owner 江苏恒康生物科技有限公司
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