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Simple wheat aphid species assay method

A bioassay and wheat aphid technology, applied in botany equipment and methods, soilless cultivation, cultivation, etc., can solve problems such as death, nutritional insecurity, and wheat aphid stylet damage, and achieve improved accuracy and strong practicability , the effect of simple operation

Inactive Publication Date: 2014-07-16
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, indoor bioassays are mainly carried out with isolated crop tissues, but it is difficult to control the humidity of isolated plant parts for short-term cultivation, freshness preservation and experiments, causing crop leaves to dry and lose water; wheat aphids have piercing-sucking mouthparts, mainly through the The needle penetrates into the plant tissue to obtain nutrients, and frequent replacement of isolated tissues can easily cause artificial damage to the wheat aphid stylet, which directly leads to the death of the wheat aphid because it cannot eat
In addition, the nutrition of the isolated part of the plant for a short period of time cannot be guaranteed, thus affecting the results of the determination

Method used

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  • Simple wheat aphid species assay method
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  • Simple wheat aphid species assay method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Determination of Life Parameters in the Pre-reproductive Stage of Aphid agriculi

[0033] Using a 160mm glass Petri dish, spread the wheat seeds evenly on the bottom of the Petri dish. Soak the seeds in clean water for 24 hours to promote the seeds to take root and germinate. After 24 hours, pour out the water in the culture dish, cover the seeds with two layers of white gauze soaked in clear water, and rinse the germinated seeds once a day through the gauze; wait until the plant grows to a plant height (from the base of the stem to the tip) of 30mm Remove the gauze from time to time, and continue to rinse the plants once a day to moisturize the roots of the plants and prevent mildew of the seeds.

[0034] When the plant grows to about 60mm in height, pull up the whole wheat seedling (be careful not to hurt the root), take 4 to 6 wheat seedlings and put their rhizomes into a 2ml centrifuge tube; It is advisable to wrap the base of the stem with moistened abs...

Embodiment 2

[0041] Implementation 2 Bioassay of Alkaloid Insecticides on Phytophthora aphid

[0042] Use a glass petri dish with a diameter of 160 mm to spread the wheat seeds evenly on the bottom of the petri dish. Soak the seeds in clean water for 24 hours to promote the seeds to take root and germinate. After 24 hours, pour out the water in the culture dish, cover the seeds with two layers of soaked white gauze, and rinse the germinated seeds once a day through the gauze; remove the gauze when the plant grows to about 25mm, and continue to rinse the plant once a day for Moisturizes the roots of plants and prevents seed mildew.

[0043] When the plant grows to about 80mm, pull up the whole wheat seedling (be careful not to hurt the root), take 3 to 4 wheat seedlings, and put their rhizomes into a 1.5ml centrifuge tube; It is advisable to wrap the base of the stem with moistened absorbent cotton and plug the opening of the centrifuge tube to keep the leaves outside the centrifuge tube ...

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Abstract

The invention relates to the field of plant protection, and provides a simple wheat aphid species assay method. The method includes A, obtaining wheat seedlings with complete roots and leaves through water planting; B, fetching a bunch of the wheat seedlings, placing the roots into an centrifugal tube, sealing the mouth of the centrifugal tube after wrapping the stem base portion through moist absorbent cotton, retaining the leaves outside the centrifugal tube, and obtaining the wheat seedlings with the centrifugal tube; C, moisturizing a culture dish provided with filter paper by spraying, and placing the wheat seedlings with the centrifugal tube; D, planting wheat aphids on the leaves of the wheat seedlings with the centrifugal tube, processing according to experiment design, and recording assay results. According to the method, during biological assay, the water-planting wheat seedlings with the roots are placed into the centrifugal tube ingeniously, the wheat seedlings can be retained in vivo during the entire experiment, and the accuracy of biological activity assay is improved greatly; according to the method, only the culture dish, the centrifugal tube and the absorbent cotton are required, cost is low, operation is simple, practicality is high, and biological activity of the object to be assayed on natural conditions can be shown better.

Description

technical field [0001] The invention belongs to the field of plant protection, and in particular relates to a simple bioassay method for wheat aphids. Background technique [0002] Wheat aphids suck the sap of wheat plants, leaves and young ears with their piercing and sucking mouthparts, and secrete honeydew to cause mold growth and affect the normal development of crops, and also spread wheat virus diseases, resulting in reduced yield and quality of crops (Li Guangbo et al., 1990) . Due to the lack of aphid-resistant varieties, the current prevention and control technology for wheat aphids mainly uses chemical control. For a long time, farmers in various places have mainly relied on chemical pesticides to prevent and control wheat aphids. Aphids have also brought about environmental pollution, increased planting costs, killing natural enemies, varying degrees of drug resistance, and re-emergence of wheat aphids (Cao Yazhong et al., 2006; Zhang Yunhui et al., 2013). There...

Claims

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Application Information

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IPC IPC(8): A01K67/033A01G31/00
Inventor 张云慧张方梅程登发殷新田李祥瑞范佳
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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