Verticillium dahliae 171 (Vd171) for preventing and treating cotton verticillium wilt and application of Vd171

A technology for Verticillium dahliae and cotton Verticillium wilt, applied in the field of phytopathology, can solve the problems of single formulation, poor control effect and the like, and achieve the effects of improved control effect and good control effect

Active Publication Date: 2012-08-15
中农科创(河南)生物科技有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Therefore, the inventor of the present invention proposes and completes the present invention in order to solve the problems that existing plant vaccines for preventing and treating cotton verticillium wilt have a single dosage form and poor control effect

Method used

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  • Verticillium dahliae 171 (Vd171) for preventing and treating cotton verticillium wilt and application of Vd171
  • Verticillium dahliae 171 (Vd171) for preventing and treating cotton verticillium wilt and application of Vd171
  • Verticillium dahliae 171 (Vd171) for preventing and treating cotton verticillium wilt and application of Vd171

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Experimental program
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Embodiment 1

[0023] The isolation of embodiment 1 bacterial strain

[0024] (1) Sample collection

[0025] From 2007 to 2009, 387 samples of cotton Verticillium wilt diseased strains were collected in 84 counties (cities) in 12 provinces including Xinjiang, Henan, Hebei, and Shandong, and 308 wild strains of cotton Verticillium wilt were isolated.

[0026] (2) Isolation of strains

[0027] First, the collected samples (cotton stalks) are washed with clear water, peeled off, placed in a sterilized petri dish with a diameter of 9 cm, and sterilized with 75% alcohol for 1 min. Then disinfect with 5.0% sodium hypochlorite for 60 seconds, and wash with sterilized water for 3 times, each time not less than 3 minutes. Then use sterilized absorbent paper to dry the water on the surface, cut into 0.4cm long pieces with sterilized scissors, place them in a 9cm petri dish, and incubate at 25°C. For the suspected Verticillium dahliae colonies, transfer them to a petri dish with a diameter of 6 cm i...

Embodiment 2VD171

[0044] Controlling effect of embodiment 2 VD171 on cotton verticillium wilt

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Abstract

The invention relates to plant pathology and particularly relates to verticillium dahliae 171 (Vd171) and an application of the Vd171 in prevention and treatment on cotton verticillium wilt. On the basis of an attenuated strain of the cotton verticillium wilt, the Vd171 is obtained through separation, screening, identification and biological assay in a greenhouse. The Vd171 has a remarkable control function on the cotton verticillium wilt, and the preservation number of the Vd171 is CGMCC (China General Microbiological Culture Collection Center) N0.5903. The Vd171 can be cultured on a synthetic medium and is easy to be obtained and cultured, thus being beneficial to industrialization production as well as promotion and application. The Vd171 has a good control effect on the verticillium, and after the Vd171 is inoculated for four days, and Vd991 is inoculated, so that the cotton verticillium wilt control efficiency can reach 85.0%. The Vd171 has the remarkable control function on the cotton verticillium wilt, and compared with bacillus subtilis usually applied in a market, the control efficiency of the Vd171 is improved by about 50%, and the Vd171 does no harm to people, livestock, insects and crops and is environment-friendly.

Description

technical field [0001] The invention relates to plant pathology, in particular to Verticillium dahlia attenuated strain Vd171 capable of preventing and treating Verticillium dahliae and its application. Background technique [0002] Cotton verticillium wilt is a soil-borne vascular disease caused by Verticillium dahliae, which is widely distributed in cotton-producing regions of the world. Since the 1990s, the disease has caused disasters in major cotton-producing areas in my country, with an annual occurrence area of ​​about 3 million hectares and an annual economic loss of about 1.2 billion yuan. It has become one of the important limiting factors for the development of cotton production. Due to the difficulty of crop rotation, the lack of disease-resistant varieties, and the lack of pesticides with good control effects, the prevention and control of Verticillium wilt in current production is almost blank. [0003] Due to the co-evolution of the interaction with the host,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
Inventor 朱荷琴冯自力李志芳师勇强赵丽红
Owner 中农科创(河南)生物科技有限公司
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