Celest coated bacterium soil nutrition pot method for authenticating cotton fusarium wilt resistance

A cotton fusarium wilt and Shileshi technology, applied in the field of phytopathology, can solve the problems of unreliable test results, poor repeatability, and poor operability, and achieve the effects of rapid and uniform water absorption, neat seedling emergence, and improved seedling emergence rate

Active Publication Date: 2013-10-09
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, according to the national standard (GB / T22101.4-2009), the paper pot soil inoculation potting method is generally used to identify the resistance of cotton varieties (lines) to Fusarium wilt. The operability is not strong and the repeatability is poor due to reasons such as Lingling seed dressing, which leads to unreliable test results.

Method used

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  • Celest coated bacterium soil nutrition pot method for authenticating cotton fusarium wilt resistance
  • Celest coated bacterium soil nutrition pot method for authenticating cotton fusarium wilt resistance
  • Celest coated bacterium soil nutrition pot method for authenticating cotton fusarium wilt resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Culture of Fusarium wilt of cotton

[0030] Cotton fusarium wilt physiological race No. 7 was selected and isolated from the Baibi experimental field of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences. On the potato dextrose agar (PDA) medium (200g of potatoes were washed, peeled and chopped, and boiled with 800mL of water for 20-30min. After gauze filtration, add 18g of glucose, 24g of agar powder, add water to make the volume to 1000mL. ) for single spore purification. Pick 2mm from the edge of the colony on the PDA plate 2 The fresh bacterial blocks were inoculated into Chapic liquid medium (NaNO 3 ,2.00g;K 2 HPO 4 ,1.00g;KCI,0.50g;MgSO 4 ·7H 2 O, 0.50g; FeSO 4, 0.01g; 30.00g sucrose; add distilled water to 1000ml), place at 25°C, 150rpm, and culture in dark for 4-5 days. Take 15ml of the above-mentioned liquid-cultured bacterial suspension and transfer it to the corn sand (mass ratio = 1:1) medium in a jar, and then put it in ...

Embodiment 2

[0054] The determination of embodiment 2 optimum inoculum concentration

[0055] The determination of the optimal inoculation concentration and appropriate inoculation in the identification of seedling stage are one of the key technologies. Since the identification of resistance to cotton wilt is often conducted in multiple batches of experiments, in order to make the results of each batch of experiments comparable, the relative Disease index was used to evaluate the disease resistance of cotton varieties (lines). When the actual disease index of the susceptible control was between 40.0 and 66.7, it was more reliable to correct the relative disease index to 50. If the inoculation concentration of fusarium wilt bacteria soil is too low, the occurrence of the disease will be reduced and the disease will be uneven, while if the concentration is too high, the emergence rate will be affected, resulting in slow emergence, and it will also cause the disease to be too severe. The actua...

Embodiment 3

[0058] The determination of the coating agent of embodiment 3 Shileshi

[0059] Cultivating healthy and neat cotton seedlings is the most basic requirement for identification of disease resistance at the seedling stage, and it is also the premise to ensure accurate identification. Under the optimal concentration of Fusarium wilt soil inoculation concentration (0.6%), in order to increase the emergence rate of cotton seedlings, the anti-seedling disease agent (1% Shilashi) was selected for coating, Jimian 11, Zhongmiansuo 45 and Zhongzhi cotton The emergence rates of No. 2 were 100%, 100% and 88.9%, respectively, slightly higher than those soaked in warm soup. The blight condition index was 52.2, 18.9 and 9.1. However, when the 5% carbendazim described in the national standard was used for seed dressing, the emergence of each variety was better, but the disease incidence was significantly reduced. The disease index of the susceptible variety Jimian 11 was only 23.1, far below ...

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Abstract

The invention relates to the field of plant pathology, in particular to a celest coated bacterium soil nutrition pot method for authenticating cotton fusarium wilt resistance. The celest coated bacterium soil nutrition pot method for authenticating cotton fusarium wilt resistance comprises the following steps that (1) pathogenic bacteria are cultivated, cotton fusarium wilt germs are inoculated into a czapek fluid medium and cultivated for 4 days to 5 days, and the germs are then transferred to a corn sandy soil medium; (2) a wilt bacterium soil nutrition pot is prepared, greensand soil is blended with a corn sandy soil culture of cotton fusarium wilt bacteria and with 06% by mass, and the greensand soil and the culture are placed in a non-bottom paper pot; (3) cotton seedings are sown and managed, sowing is conducted with 1% celest coating, and cotton seedings are cultivated in a solar greenhouse with temperature and humidity controllable; (4) cotton fusarium wilt resistance is evaluated, disease happening conditions are investigated in a classified mode, and disease indexes are calculated. The celest coated bacterium soil nutrition pot method for authenticating cotton fusarium wilt resistance has the advantages of being rapid, accurate, economical and the like.

Description

technical field [0001] The invention relates to the field of phytopathology, in particular, the invention relates to a Shilashie-coated bacterial soil nutrient pot method for identifying cotton wilt resistance. Background technique [0002] Cotton Fusarium Wilt is a worldwide dangerous disease. It is listed as the two major diseases of cotton together with Verticillium wilt, which seriously affects cotton production and is also a quarantine object for cotton entry. Breeding disease-resistant varieties is one of the most economical and effective measures to control cotton wilt. Fusarium wilt resistance identification of cotton varieties and resource materials is the basic work of disease resistance breeding. Because Fusarium wilt can cause disease at the cotyledon stage, researchers often carry out the identification of Fusarium wilt resistance at the cotton seedling stage. In the early days, the seedbed disease nursery method was mainly used, but because the establishment ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00
Inventor 李志芳朱荷琴冯自力赵丽红师勇强
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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