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Endophytic fungi CEF-193 of cotton and application thereof

A technology of CEF-193 and endophytic fungi, applied to endophytic fungus CEF-193 and its application fields, can solve the problems of poor cotton verticillium wilt effect, achieve good induction of immunity, and be conducive to mass reproduction and popularization and application Effect

Active Publication Date: 2014-07-02
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem that the existing antagonistic microorganisms are less effective in controlling cotton verticillium wilt, the present invention is proposed and completed

Method used

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  • Endophytic fungi CEF-193 of cotton and application thereof
  • Endophytic fungi CEF-193 of cotton and application thereof
  • Endophytic fungi CEF-193 of cotton and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The isolation of embodiment 1 bacterial strain

[0023] (1) Sample collection

[0024] From June to August, healthy cotton plants were selected, and the middle and upper parts of the main stem and fruiting branches were taken 15 cm.

[0025] (2) Isolation of strains

[0026] First, the collected samples (cotton stalks) are washed with clear water, peeled off, placed in a sterilized petri dish with a diameter of 9 cm, and sterilized with 75% alcohol for 1 min. Then disinfect with 5.0% sodium hypochlorite for 60 seconds, and wash with sterilized water for 3 times, each time not less than 3 minutes. Then use sterilized absorbent paper to dry the water on the surface, cut into 0.4cm long pieces with sterilized scissors, place them in a 9cm petri dish, and incubate at 25°C. Use the last cleaning solution as a control to check whether the disinfection is thorough. Check the growth of endophytic fungi every day, and find that there are colonies growing out, and transfer th...

Embodiment 2

[0035] The inhibitory effect of embodiment 2CEF-193 on Verticillium dahliae of cotton

[0036] 1. Test method

[0037] Buckle cultivation method. Pour a certain amount of culture medium into the lid and bottom of the 90mm petri dish respectively, and the culture medium in the lid is slightly less. After solidification, insert 5 μl of Acremonium aromaticum CEF-193 bacterium liquid in the center of the dish cover (7 days of Chapi liquid culture), and inoculate 5 μl of Verticillium dahliae bacterial liquid in the center of the bottom of the dish (7 days of Chapi liquid culture). Put them into a constant temperature incubator at 25°C, and culture them upside down. Take the petri dish inoculated only with pathogenic bacteria as a control, and repeat each treatment 4 times. The growth status of the pathogenic bacteria and Acremonium aromaticum hyphae was observed every day, and the colony diameter of the pathogenic bacteria was measured by the cross method after 7 days of button c...

Embodiment 3

[0044] Example 3 CEF-193's Induced Immunity to Cotton Verticillium Wilt

[0045] 1. Test method

[0046] CEF-193 was cultured in Chapic liquid medium at 25°C and shaken in the dark for 7 days, filtered through filter paper, and the culture filtrate was obtained for seed soaking. The control strain Vd080 (strong pathogenic strain of Verticillium dahliae) was cultured in Chapic liquid medium at 25°C in the dark for 7 days with shaking, filtered through three layers of gauze, and the spore suspension was obtained for inoculation. Four treatments were set up in the experiment, respectively 25%, 50% and 100% CEF-193 culture filtrate and clear water soaking seeds. All four treatments were inoculated with conidia suspension of Verticillium dahliae Vd080 when a true leaf of cotton seedlings was flattened.

[0047] Mix the sterilized vermiculite and sand at a ratio of 6:4 to make a bottomless paper pot with a diameter of 6 cm, and place it in a plastic tray of 45 cm × 35 cm. The susc...

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Abstract

The invention relates to phytopathology, and particularly relates to endophytic fungi CEF-193 of cotton and application thereof. The bacterial strain CEF-193 (Acremonium alternatum) with a significant induced immune function on cotton verticillium wilt is obtained from the endophytic fungi of the cotton by separation, screening, identifying and biological assay of a greenhouse, the preservation number is CGMCC No.8302, and CEF-193 can be cultivated on an artificial medium, and is easily obtained and cultivated. Thus, industrialized production and popularization and application are facilitated. An indoor bacteriostatic test shows that the control effect on greensickness is good, verticillium dahlia is inoculated 4 days after the CEF-193 is firstly inoculated, the prevention and control effect on the cotton verticillium wilt achieves 74.1%, and the endophytic fungi CEF-193 have significant control action on the greensickness. Compared with the bacillus subtilis mainly applied to production, the prevention and control effect is improved by about 40%, and the endophytic fungi CEF-193 are safe on people and livestock, insects and crops, and friendly to environment.

Description

technical field [0001] The present invention relates to plant pathology, in particular to cotton endophytic fungus CEF-193 and its application. Background technique [0002] Verticillium dahliae caused by Verticillium dahliae is one of the important diseases of cotton worldwide. Due to the lack of truly effective disease-resistant varieties or the resistance of disease-resistant varieties is easily lost, the most economical and effective control measures cannot play its leading role. Since the 1990s, the disease has become a disaster in major cotton areas in my country, with an annual occurrence area of ​​about 3 million hectares and a loss of about 100 million kilograms of lint, which has become one of the main limiting factors for the development of cotton production. Due to the difficulty of crop rotation, the lack of disease-resistant varieties, the poor control effect of chemical agents, and the lack of pesticides with good control effects, the current production of Ve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
Inventor 冯自力朱荷琴李志芳黄殿成王玲飞师勇强赵丽红
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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