Cotton endophytic bacteria YUPP-10 and application thereof in cotton verticillium wilt controlling
A kind of YUPP-10, cotton verticillium wilt technology, applied to cotton endogenous bacteria YUPP-10 and its application field in cotton verticillium wilt prevention and treatment, to achieve the effects of convenient use, short production cycle and low production cost
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Embodiment 1
[0021] The isolation of embodiment 1 bacterial strain
[0022] (1) Isolation of strains
[0023] Collect phenotypically disease-free cotton plants in cotton fields, clean the surface of the cotton plants, peel off the skin, and cut them into 2-3cm long sections. The following steps are completed in an ultra-clean workbench. Disinfect the peeled cotton plants with 70% ethanol for 1 min, wash 3 times with sterile water, sterilize with 3% sodium hypochlorite for 4 min, wash 3 times with sterile water, and sterilize the cotton stalks Cut into pieces and grind, make a suspension with sterile water, spread it on an LB plate, incubate at 37°C for 24 hours, pick a single colony, pick a single colony with differences according to its shape, size, color, etc. Cultivate at 37°C for 24 hours on the collection medium plate, and select the bacteria that can grow on the enrichment medium for further pure culture. The purified strains were preserved.
[0024] (2) Characteristics of YUPP-10...
Embodiment 2
[0032] Example 2 The inhibitory effect of YUPP-10 on Verticillium dahliae
[0033] (1) Test method
[0034] 1. Inhibitory effect of whole metabolites of YUPP-10 on Verticillium dahliae
[0035] Introduce YUPP-10 into LB liquid medium, shake and culture in a constant temperature shaker at 37°C and 180rpm / min for 24h. Inoculate Verticillium dahliae in the center of different PDA plate culture medium, punch holes in the PDA plate symmetrically in a triangular shape, each point is 20 mm away from the center, add 100 μL of YUPP-10 culture medium to two points, and add 100 μL of LB liquid to the remaining point The culture medium was used as a control, cultured at a constant temperature of 25°C to observe the antibacterial situation, and the colony radius of Verticillium dahliae was measured after 7 days. The following formula was used to calculate the relative inhibition rate of YUPP-10 on the growth of pathogenic fungi, and each treatment was repeated three times.
[0036] Colo...
Embodiment 3
[0048] Example 3 The control effect of YUPP-10 on cotton verticillium wilt
[0049] (1) Test method
[0050] 1. Preparation of YUPP-10 bacterial fertilizer
[0051] The preserved YUPP-10 was inoculated on LB solid medium, and activated at 37°C for 18 hours; the activated strain was inoculated in LB liquid medium, shaken and cultured at 37°C, 180rpm / min for 18h to make strains liquid; inoculate the YUPP-10 strain liquid in sterilized vermiculite corn culture medium Kernel flasks, culture at 37°C for 72 hours, take out the culture, and air-dry it in a cool place to make YUPP-10 bacterial fertilizer for use.
[0052] 2. Cultivation of cotton seedlings
[0053] The cotton seeds were sterilized with 10% hydrogen peroxide for 10 minutes, washed three times with sterile water, and soaked in sterile water at room temperature for 24 hours. The disease-resistant variety Lumianyan No. 21 was used as the test variety. The cotton seedling cultivation method refers to the vermiculite san...
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