39results about How to "High survival rate of live bacteria" patented technology

The invention discloses a manufacturing method and a probiotic tablet with high living bacterium survival rate manufactured by applying the method; the manufacturing method is as follows: applying a dry method pelleting technique and a tabletting technique and taking auxiliary materials such as glucose, lactin, microcrystalline cellulose and the like as raw material to prepare particles, and thenblending active ingredient probiotic powder and magnesium stearate to carry out tabletting, and finally obtaining the probiotic tablet. The invention has the advantages that the probiotic tablet is convenient to take and absorb and has obvious treatment effect owing to the high probiotic survival rate; the dry method pelleting technology provided by the invention has the advantages of low cost, fast discharging speed, stable technological conditions, good recurrence and high probiotic survival rate and is suitable for large-scale production.

The invention discloses a production method for microbial germ powder, which has the technical steps that: the heat treatment is carried out on proper production seeds after primary seed culture at the treatment temperature being 60 to 90 DEG C for 5 to 60 minutes, the heat treatment is carried out after secondary seed culture, the heat treatment and the cold treatment are carried out after tertiary seed culture, then, the materials are inoculated into a liquid culture medium and is cultured for 16 to 36 hours in a fermentation tank, a liquid culture medium and / or alkali are / is supplemented into the fermentation tank in real time according to the real-time data dynamically detected in the fermentation culture process, germ sledge is obtained after fermentation liquid is separated, the germ sledge is uniformly mixed with absorbing agents, and germ powder is obtained after the crushing and the sieving. The method has the advantages that the viable count of the fermentation liquid is greatly improved, the fermentation factor and the yield are high, the energy consumption is low, the cost is low, the product quality standard is improved, microbes realize the synchronously growth in the fermentation culture process of the seeds after the heat treatment and cold treatment, the viable count of the obtained germ powder is high, the spore mature speed is high, the spore conversion rate is high, the fermentation level is high, and the viable germ yield and the survival rate are high.

The invention relates to a bacterial agent for preventing and treating white stool syndrome of Litopenaeus vannamei and its preparation method and application. The bacterial agent of the invention is a fermented bacterial powder prepared by spray-drying the fermented liquid of Enterococcus faecalis. The preparation method of the bacterial agent for preventing and treating white stool syndrome of Litopenaeus vannamei of the present invention comprises the following steps of successively drying Enterococcus faecalis through shake flask culture, first-level seed culture, second-level seed culture and fermentation culture to obtain the control Inoculum of Litopenaeus vannamei white stool syndrome. The application of the bacterial agent for preventing and treating white stool syndrome of Litopenaeus vannamei according to the present invention is to mix the bacterial agent into the feed according to the ratio, and then use the feed to feed the common bacteria in ponds that produce (treat) or do not produce (prevent) white stool. Lime prawns. The Enterococcus faecalis bacterial agent of the present invention can significantly inhibit the growth and reproduction of pathogenic bacteria such as Vibrio cholerae, Edwardsiella tarda, Aeromonas hydrophila, etc., has broad-spectrum antibacterial properties, can prevent and treat white stool disease of Litopenaeus vannamei, and has a good control effect Reached more than 78%, the effect is remarkable.

The invention discloses a method for culturing microbes through fermentation. The method comprises the following process steps of: performing one-stage seed culture on proper bacillus produced seeds, and performing heat treatment at the temperature of between 60 and 90DEG C for 5 to 60 minutes; performing two-stage seed culture and heat treatment; performing three-stage seed culture, heat treatment and cold treatment; inoculating three-stage seeds subjected to cold treatment into a liquid medium, and culturing in a fermentation tank for 16 to 36 hours; and dynamically detecting real-time data in the fermentation culture process and supplying a liquid medium and / or alkali to the fermentation tank in real time. By the method, the viable count and the fermentation coefficient of fermentation liquor are greatly improved, the yield is high, the energy consumption is low, the cost is low, the product quality standard is improved, after the seeds are subjected to heat treatment and cold treatment, the microbes synchronously grow in the fermentation culture process, the obtained microbe powder has high viable count, spores are quickly matured, the spore conversion rate is high, the fermentation level is high, the yield of viable bacteria is high, and the survival rate of the viable bacteria is high.