The invention belongs to the field of aquaculture, and particularly relates to tablets for improving the culture environment of a culture pond as well as a preparation method and application of the tablets. The tablets comprise the following raw materials: a bacillus subtilis solid culture and a bacillus amyloliquefaciens solid culture, and further comprise the bacillus subtilis solid culture, thebacillus amyloliquefaciens solid culture, anhydrous sodium sulphate, sodium humate, dolomite powder, zeolite powder, maltodextrin and magnesium stearate. Research shows, in a process of preparing thetablets from the raw materials, a granulation process is not required, direct tabletting is performed, the oxidation-reduction potential and the dissolved oxygen at the bottom of the tank can be quickly improved; the sludge at the bottom of the pond is decomposed, residual feed, excrement, animal and plant corpses and organic chippings at the bottom of the pond are degraded, harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide and algal toxin at the bottom of the pond can be effectively adsorbed, the acidity of water is reduced, the pH value of the water is stabilized, microelements are supplemented, the transparency of the water is improved, and the purposes of improving substrate and purifying water are achieved.