Method for efficiently producing carotenoid

A carotene and high-efficiency technology, applied in the field of carotenoid preparation, to achieve the effect of increasing the extraction rate, improving the survival rate of live bacteria, and improving the ability to resist cold and heat stress

Inactive Publication Date: 2016-12-14
BEIJING KEHUITONG WISDOM SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The technical problem solved by the present invention is to overcome the defects of the existing fermentation preparation of carotenoids, and use the leftovers of canned yellow peaches as the main raw material to obtain fruit juice with high carotenoid content through microwave solidification, freezing and crushing, and biological enzymatic hydrolysis , after adjusting the components to obtain the fermentation medium, inoculate the marine red yeast with high carotenoid production into it for staged inoculation and variable temperature fermentation, so as to maximize the proliferation of marine red yeast, and the fermentation liquid is homogenized by ultra-high pressure, vacuum centrifuged, and demulsified After separation, the emulsion is obtained, and the emulsion is extracted, separated and purified to obtain the finished carotenoids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A method for producing carotenoids by fermenting yellow peach skin, comprising the steps of:

[0044] 1) Juice preparation: first, put the yellow peach peel into a microwave dryer at a power of 4kW, a material layer thickness of 3cm, and dry at 110°C for 1.5min, then soak it in a sericin peptide solution with a mass percentage of 12% for 12min, and dry it at - Immediately pulverize after freezing at 20°C for 30 minutes, the thickness of the frozen material layer is 4cm, and the particle size of the pulverized material is 0.4mm, then add water twice the amount of the pulverized material, and adjust the pH value to 4.5 with lactic acid. Under the conditions of pulse time 400μs and pulse frequency 250Hz, conduct high-voltage pulse electric field treatment for 25min; then conduct microwave irradiation extraction at room temperature under the condition of power 200W for 18min, and at the same time conduct ultrasonic-assisted extraction under the condition of power 250W and fr...

Embodiment 2

[0058] A method for producing carotenoids by fermenting defective yellow peach pulp, comprising the steps of:

[0059] 1) Juice preparation: first slice the defective yellow peach pulp, put it into a microwave dryer with a power of 3kW, a material layer thickness of 2cm, and dry at 105°C for 1min, and then soak it in a sericin peptide solution with a mass percentage of 10% for 10min , After freezing at -18°C for 20 minutes, crush immediately. The thickness of the frozen material layer is 3 cm, and the particle size of the crushed product is 0.3 mm. Then add water that is 1 times the amount of the crushed product, and adjust the pH value to 3.5 with lactic acid. / cm, pulse time 300μs, pulse frequency 200Hz under the conditions of high-voltage pulse electric field treatment for 20min; then microwave irradiation extraction at room temperature under the condition of power 150W for 15min, and ultrasonic-assisted extraction under the condition of power 200W and frequency 30KHz; Mixe...

Embodiment 3

[0073] A method for efficiently producing carotenoids, comprising the steps of:

[0074] 1) Juice preparation: First, slice the defective yellow peach pulp into slices, then evenly mix it with the yellow peach skin at a mass ratio of 3:1, put it into a microwave dryer at a power of 5kW, a material layer thickness of 4cm, and dry at 115°C for 2 minutes, then Soak in sericin peptide solution with a mass percentage of 14% for 15 minutes, freeze at -22°C for 40 minutes, and then crush immediately. The thickness of the frozen material layer is 5 cm, and the particle size of the crushed product is 0.5 mm. Lactic acid adjusts the pH value to 5.5, and conducts high-voltage pulse electric field treatment at room temperature under the conditions of electric field strength 35kV / cm, pulse time 500μs, and pulse frequency 300Hz for 30min; Under the conditions of power 300W and frequency 40KHz, ultrasonic-assisted extraction is carried out; mixed enzymes with 2.5% of the quality of the extra...

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Abstract

The invention discloses a method for efficiently producing carotenoid. The method comprises the steps that juice with the high carotenoid content is obtained by taking canned yellow peach leftovers as main raw materials through microwave color fixing, frozen-breaking and biological enzymolysis, and after the ingredients are regulated, a fermentation medium is obtained; fractional inoculation and temperature-variable fermentation are conducted on oceanic red yeast inoculated with the high-yield carotenoid, so that the oceanic red yeast is maximumly proliferated; after fermentation liquid is subjected to ultrahigh pressure homogenizing, vacuum centrifuging and demulsifcation separating, emulsion is obtained, and after the emulsion is extracted, separated and purified, the finished carotenoid is obtained, wherein the cell biomass of the fermentation liquid is 38-52 g/L, and the yield of the carotenoid is 47-61 mg/L. The preparation method is simple, the cycle is short, the efficiency is high, energy is saved, environmental friendliness is achieved, a low-temperature bio-processing technique is adopted in the whole process, the production cost is reduced, hidden environmental pollution danger is eliminated, a firm foundation is laid for sustainable development of the field of yellow peach deep processing technologies, and the positive economic and social development significance is achieved.

Description

technical field [0001] The invention relates to the preparation of carotenoids, in particular to a method for efficiently producing carotenoids. Background technique [0002] Carotenoids are a general term for a group of fat-soluble pigments with antioxidant properties. As a class of important biologically active substances in humans and animals, carotenoids have important functions such as enhancing the body's immunity, anti-radiation, anti-tumor, and treating photosensitivity diseases. , It has a wide range of applications in food, cosmetics, medicine and feed industries. At present, carotenoids have been recognized as Class A nutritional pigments by international organizations such as FAO, European Community and WHO, and in recent years, the industrialization development of carotenoids is gradually taking shape. [0003] The industrial production method of carotenoids can be chemical synthesis or plant extraction. The chemical synthesis of carotenoids is not only techni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12R1/645
CPCC12P23/00
Inventor 李建树
Owner BEIJING KEHUITONG WISDOM SCI & TECH CO LTD
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