Method for efficiently producing carotenoid

Abstract

The invention discloses a method for efficiently producing carotenoid. The method comprises the steps that juice with the high carotenoid content is obtained by taking canned yellow peach leftovers as main raw materials through microwave color fixing, frozen-breaking and biological enzymolysis, and after the ingredients are regulated, a fermentation medium is obtained; fractional inoculation and temperature-variable fermentation are conducted on oceanic red yeast inoculated with the high-yield carotenoid, so that the oceanic red yeast is maximumly proliferated; after fermentation liquid is subjected to ultrahigh pressure homogenizing, vacuum centrifuging and demulsifcation separating, emulsion is obtained, and after the emulsion is extracted, separated and purified, the finished carotenoid is obtained, wherein the cell biomass of the fermentation liquid is 38-52 g / L, and the yield of the carotenoid is 47-61 mg / L. The preparation method is simple, the cycle is short, the efficiency is high, energy is saved, environmental friendliness is achieved, a low-temperature bio-processing technique is adopted in the whole process, the production cost is reduced, hidden environmental pollution danger is eliminated, a firm foundation is laid for sustainable development of the field of yellow peach deep processing technologies, and the positive economic and social development significance is achieved.

Description

technical field [0001] The invention relates to the preparation of carotenoids, in particular to a method for efficiently producing carotenoids. Background technique [0002] Carotenoids are a general term for a group of fat-soluble pigments with antioxidant properties. As a class of important biologically active substances in humans and animals, carotenoids have important functions such as enhancing the body's immunity, anti-radiation, anti-tumor, and treating photosensitivity diseases. , It has a wide range of applications in food, cosmetics, medicine and feed industries. At present, carotenoids have been recognized as Class A nutritional pigments by international organizations such as FAO, European Community and WHO, and in recent years, the industrialization development of carotenoids is gradually taking shape. [0003] The industrial production method of carotenoids can be chemical synthesis or plant extraction. The chemical synthesis of carotenoids is not only techni...

AI Technical Summary

Problems solved by technology

[0014] The technical problem solved by the present invention is to overcome the defects of the existing fermentation preparation of carotenoids, and use the leftovers of canned yellow peaches as the main raw material to obtain fruit juice with high carotenoid content through microwave solidification, freezing and crushing, and biological enzymatic hydrolysis , after adjusting the components to obtain the fermentation medium, inoculate the marine red yeast with high carotenoid production into it for staged inoculation and variable temperature fermentation, so as to maximize the proliferation of marine red yeast, and the fermentation liquid is homogenized by ultra-high pressure, vacuum centrifuged, and demulsified After separation, the emulsion is obtained, and the emulsion is extracted, separated and purified to obtain the finished carotenoids